##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364092_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2238858 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.538495072041194 31.0 31.0 34.0 27.0 34.0 2 30.61316617668472 31.0 31.0 34.0 27.0 34.0 3 30.669654797222513 31.0 31.0 34.0 27.0 34.0 4 34.14169009378889 37.0 35.0 37.0 30.0 37.0 5 34.181728363299506 37.0 35.0 37.0 30.0 37.0 6 34.201998518887756 37.0 35.0 37.0 30.0 37.0 7 34.1065645967721 37.0 35.0 37.0 30.0 37.0 8 34.08313256133261 37.0 35.0 37.0 30.0 37.0 9 35.55633541743156 39.0 35.0 39.0 30.0 39.0 10-11 35.47134342597878 39.0 35.0 39.0 30.0 39.0 12-13 35.374235212773655 39.0 35.0 39.0 29.0 39.0 14-15 36.56861020216557 40.0 36.5 41.0 29.0 41.0 16-17 36.566338731621215 40.0 36.5 41.0 29.0 41.0 18-19 36.55826229265099 40.0 36.5 41.0 29.0 41.0 20-21 36.57376774230434 40.0 37.0 41.0 29.0 41.0 22-23 36.50812624114616 40.0 37.0 41.0 29.0 41.0 24-25 36.50684657088569 40.0 36.5 41.0 29.0 41.0 26-27 36.45881404716154 40.0 36.0 41.0 29.0 41.0 28-29 36.40378867261792 40.0 36.0 41.0 29.0 41.0 30-31 36.353390210544845 40.0 36.0 41.0 29.0 41.0 32-33 36.25256380708379 40.0 36.0 41.0 29.0 41.0 34-35 36.22953219900503 40.0 36.0 41.0 29.0 41.0 36-37 36.16695163337737 40.0 36.0 41.0 29.0 41.0 38-39 36.0889712523081 40.0 36.0 41.0 28.0 41.0 40-41 36.015383289159026 40.0 35.5 41.0 28.0 41.0 42-43 35.90073488358797 40.0 35.0 41.0 28.0 41.0 44-45 35.800554568445165 40.0 35.0 41.0 27.0 41.0 46-47 35.6998827080592 40.0 35.0 41.0 27.0 41.0 48-49 35.596247506541275 39.5 35.0 41.0 27.0 41.0 50-51 35.47449145948515 39.0 35.0 41.0 27.0 41.0 52-53 35.33929798138158 39.0 35.0 41.0 26.0 41.0 54-55 35.170312498604204 39.0 35.0 41.0 26.0 41.0 56-57 34.984140798567836 39.0 34.0 41.0 26.0 41.0 58-59 34.78296770049731 38.5 34.0 40.5 26.0 41.0 60-61 34.543884873448874 38.0 34.0 40.0 25.0 41.0 62-63 34.28821099864305 38.0 34.0 40.0 25.0 41.0 64-65 34.01762237712262 37.0 33.5 40.0 24.0 41.0 66-67 33.7115674598389 37.0 33.0 40.0 23.0 41.0 68-69 33.3987523996609 36.0 33.0 39.0 23.0 41.0 70-71 33.068235904197586 36.0 33.0 39.0 22.5 41.0 72-73 32.72081525492014 35.0 32.5 38.5 22.0 40.0 74-75 32.44793863657276 35.0 32.0 37.5 22.0 40.0 76-77 32.224422897745185 35.0 32.0 37.0 22.0 39.0 78-79 31.92764101162289 35.0 32.0 37.0 21.5 39.0 80-81 31.724164060427235 35.0 32.0 36.0 21.5 39.0 82-83 31.630936620366278 35.0 32.0 36.0 22.0 37.0 84-85 31.41734513756567 35.0 32.0 35.5 21.5 37.0 86-87 31.227247552100224 35.0 32.0 35.0 20.0 36.5 88-89 31.04978363969488 35.0 32.0 35.0 20.0 36.0 90-91 30.910804749564285 35.0 32.0 35.0 20.0 36.0 92-93 30.794562004379017 35.0 32.0 35.0 19.5 36.0 94-95 30.695968435693555 35.0 32.0 35.0 19.0 35.5 96-97 30.61137508497636 35.0 32.0 35.0 18.0 35.0 98-99 30.537297809865564 35.0 32.0 35.0 18.0 35.0 100-101 30.467561587202045 35.0 32.0 35.0 17.0 35.0 102-103 29.35807340170748 33.5 29.5 34.5 10.0 35.0 104-105 30.20024673293259 34.5 31.5 35.0 11.0 35.0 106-107 30.308628774133958 35.0 32.0 35.0 10.0 35.0 108-109 30.295018710431833 35.0 32.0 35.0 7.5 35.0 110-111 30.229142268067022 35.0 32.0 35.0 4.5 35.0 112-113 30.1554511273158 35.0 32.0 35.0 2.0 35.0 114-115 30.064015225619492 35.0 32.0 35.0 2.0 35.0 116-117 29.971812415079473 35.0 32.0 35.0 2.0 35.0 118-119 29.849363827451317 35.0 32.0 35.0 2.0 35.0 120-121 29.710510671065336 35.0 32.0 35.0 2.0 35.0 122-123 29.545273751171358 35.0 31.0 35.0 2.0 35.0 124-125 28.694213299816244 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5528334481365533 1101 2 0.5169251671139641 1101 3 0.49340353301507633 1101 4 0.5791857302436227 1101 5 0.5901998257415002 1101 6 0.5262300242148967 1101 7 0.5341156479002365 1101 8 0.4708862847542221 1101 9 0.4500269248118727 1101 10-11 0.5822467003703622 1101 12-13 0.6076747893319023 1101 14-15 0.6636352683781368 1101 16-17 0.627993986003986 1101 18-19 0.6178868967610569 1101 20-21 0.6320130059996103 1101 22-23 0.7748828110582835 1101 24-25 0.7704818445342667 1101 26-27 0.6302442504504313 1101 28-29 0.7267642565201342 1101 30-31 0.7249771981989426 1101 32-33 0.6588239219260146 1101 34-35 0.7641444076393569 1101 36-37 0.6871977526264175 1101 38-39 0.6163637515350686 1101 40-41 0.7456857239848134 1101 42-43 0.7236219978926925 1101 44-45 0.7671214518955694 1101 46-47 0.7192565936653921 1101 48-49 0.6032082568229455 1101 50-51 0.7248791961681462 1101 52-53 0.6680027030120073 1101 54-55 0.7109437972073707 1101 56-57 0.7108952713569394 1101 58-59 0.6527938348656477 1101 60-61 0.7428391512349108 1101 62-63 0.7334368291325575 1101 64-65 0.7023721939290581 1101 66-67 0.7185046010078722 1101 68-69 0.6471069358836061 1101 70-71 0.6742184710680306 1101 72-73 0.7698597955470774 1101 74-75 0.8244519607015732 1101 76-77 0.6675850731199233 1101 78-79 0.6964164135722299 1101 80-81 0.6847566988159812 1101 82-83 0.642824179709887 1101 84-85 0.7294433125363895 1101 86-87 0.6819028948903743 1101 88-89 0.6826179332406959 1101 90-91 0.6737366594196743 1101 92-93 0.7474009504243675 1101 94-95 0.7586064117350553 1101 96-97 0.7018051210536669 1101 98-99 0.6255381399297875 1101 100-101 0.6932798131569378 1101 102-103 0.7590098379565298 1101 104-105 0.6953963336318871 1101 106-107 0.7382962044641417 1101 108-109 0.6804175923624527 1101 110-111 0.686898667525945 1101 112-113 0.7036761343843096 1101 114-115 0.7343008309901435 1101 116-117 0.7227516461546486 1101 118-119 0.771732944625267 1101 120-121 0.846417806470658 1101 122-123 0.8223876649732098 1101 124-125 0.8351847064790121 1102 1 0.35914147044457323 1102 2 0.4659466281354234 1102 3 0.37332318893473015 1102 4 0.4382277172856064 1102 5 0.47787343341510535 1102 6 0.3873552373401097 1102 7 0.5130563468409406 1102 8 0.4710667729347122 1102 9 0.49980399858894486 1102 10-11 0.5265577366813972 1102 12-13 0.4742256918828005 1102 14-15 0.48633695907982855 1102 16-17 0.6112277692377717 1102 18-19 0.48352308239724806 1102 20-21 0.6522648182514246 1102 22-23 0.6897480939235692 1102 24-25 0.6915932856457019 1102 26-27 0.6818577980639731 1102 28-29 0.7057193874752627 1102 30-31 0.701234730456477 1102 32-33 0.6403765514786457 1102 34-35 0.6426653901603387 1102 36-37 0.6561134815421426 1102 38-39 0.6324811436524627 1102 40-41 0.7149914252905134 1102 42-43 0.6640843063550079 1102 44-45 0.7417866665607775 1102 46-47 0.8031785375873284 1102 48-49 0.7591655327802229 1102 50-51 0.7876287589177124 1102 52-53 0.8137319047412106 1102 54-55 0.7171629394265153 1102 56-57 0.7635996240612997 1102 58-59 0.7763314824032932 1102 60-61 0.8201015564973133 1102 62-63 0.7322009558966869 1102 64-65 0.6738470094038718 1102 66-67 0.782382544885813 1102 68-69 0.7616701144467797 1102 70-71 0.706086690936246 1102 72-73 0.7731401748274607 1102 74-75 0.7559608762104872 1102 76-77 0.7017658473006918 1102 78-79 0.7702021993580175 1102 80-81 0.6485399985992757 1102 82-83 0.6202597251454272 1102 84-85 0.6499868890799689 1102 86-87 0.6529257419132257 1102 88-89 0.6954063700291293 1102 90-91 0.6785829122659237 1102 92-93 0.6210798161032329 1102 94-95 0.6383963555249998 1102 96-97 0.6728973561459028 1102 98-99 0.6958024701941206 1102 100-101 0.6918443117214359 1102 102-103 0.7600640350107248 1102 104-105 0.7166663949019494 1102 106-107 0.6956551898231282 1102 108-109 0.7127526086974676 1102 110-111 0.6616153622426353 1102 112-113 0.6927396594478346 1102 114-115 0.8110811277704357 1102 116-117 0.7722248996279042 1102 118-119 0.7210799179722436 1102 120-121 0.6842018762547291 1102 122-123 0.68725059383614 1102 124-125 0.6983654696597767 1103 1 0.4655242344455033 1103 2 0.47050491692914775 1103 3 0.42533598093198677 1103 4 0.33219834880748067 1103 5 0.40354130624760387 1103 6 0.3509255767264037 1103 7 0.38224467469672874 1103 8 0.41172199762459627 1103 9 0.5274114303409831 1103 10-11 0.5395594076083725 1103 12-13 0.48733620544387435 1103 14-15 0.716280767244136 1103 16-17 0.6965523827667468 1103 18-19 0.6555907162097228 1103 20-21 0.6036668255769442 1103 22-23 0.6155831922426884 1103 24-25 0.6039512845170023 1103 26-27 0.7472178662289437 1103 28-29 0.6910803851625218 1103 30-31 0.7844564547203277 1103 32-33 0.5100786838470484 1103 34-35 0.6809293523597617 1103 36-37 0.7335896072047063 1103 38-39 0.761421864474876 1103 40-41 0.8453968519301824 1103 42-43 0.823069046697384 1103 44-45 0.6937011505099164 1103 46-47 0.8558441640742913 1103 48-49 0.794307893545529 1103 50-51 0.7488709990664404 1103 52-53 0.6772377972485941 1103 54-55 0.7919195927036284 1103 56-57 0.8244468172359518 1103 58-59 0.8415900998495403 1103 60-61 0.889268328288523 1103 62-63 0.8317122042229954 1103 64-65 0.7462715797764545 1103 66-67 0.8021705252773117 1103 68-69 0.8176695832911847 1103 70-71 0.8008578182033403 1103 72-73 0.769358391251231 1103 74-75 0.6928097513778582 1103 76-77 0.7187800889100942 1103 78-79 0.6862378753088407 1103 80-81 0.6932952133299963 1103 82-83 0.6329310172920763 1103 84-85 0.5839140543689467 1103 86-87 0.5987671698365169 1103 88-89 0.6243859537359349 1103 90-91 0.619093618370897 1103 92-93 0.5940799378263897 1103 94-95 0.6606895755451383 1103 96-97 0.6586050213850925 1103 98-99 0.7115538893754767 1103 100-101 0.7096712213917904 1103 102-103 0.660072046607624 1103 104-105 0.7033239876605286 1103 106-107 0.7329282812593476 1103 108-109 0.7036244342128555 1103 110-111 0.6677256434231325 1103 112-113 0.6670094200958445 1103 114-115 0.686919966310203 1103 116-117 0.6641377901102601 1103 118-119 0.6847550277997279 1103 120-121 0.6857356765623557 1103 122-123 0.6983120861620478 1103 124-125 0.7533688447109554 1104 1 0.3533869438193342 1104 2 0.4036943843101213 1104 3 0.36517584255046387 1104 4 0.4406959919342768 1104 5 0.44415970766289803 1104 6 0.29027837936367007 1104 7 0.4469268619742337 1104 8 0.3895582987359205 1104 9 0.4755868096621967 1104 10-11 0.48447386327100617 1104 12-13 0.4710060753931984 1104 14-15 0.551381568331287 1104 16-17 0.5241539846895407 1104 18-19 0.5854377390864087 1104 20-21 0.5222586268525689 1104 22-23 0.4890859260431242 1104 24-25 0.5866350441811505 1104 26-27 0.5763121754720544 1104 28-29 0.6629520103567188 1104 30-31 0.6420646897001347 1104 32-33 -0.06976691971352977 1104 34-35 0.5472043536578113 1104 36-37 0.5255653868449244 1104 38-39 0.6858825378307714 1104 40-41 0.7398913607039788 1104 42-43 0.733939498889697 1104 44-45 0.7076168262454203 1104 46-47 0.7914830296921878 1104 48-49 0.6878223555681302 1104 50-51 0.8097607827033713 1104 52-53 0.6865956716951729 1104 54-55 0.8113431581298798 1104 56-57 0.7521811701293117 1104 58-59 0.7617076210193545 1104 60-61 0.8082260526109835 1104 62-63 0.8393829340263466 1104 64-65 0.8691142417594975 1104 66-67 0.7712665341065374 1104 68-69 0.8447287100237162 1104 70-71 0.8422782679612624 1104 72-73 0.7895936168443356 1104 74-75 0.8315345605382376 1104 76-77 0.7330065769058223 1104 78-79 0.6858891996475407 1104 80-81 0.6928928192491455 1104 82-83 0.6897407612025397 1104 84-85 0.7470238370321312 1104 86-87 0.7505744802486731 1104 88-89 0.690863575882009 1104 90-91 0.6115899276998817 1104 92-93 0.5822508813356322 1104 94-95 0.5559630912324423 1104 96-97 0.6038312774394186 1104 98-99 0.5999696379632091 1104 100-101 0.5535901525713065 1104 102-103 0.5515017758182843 1104 104-105 0.5805344602011644 1104 106-107 0.515842446231666 1104 108-109 0.4846359520528516 1104 110-111 0.4823202463209455 1104 112-113 0.5975176376665843 1104 114-115 0.5716815598320864 1104 116-117 0.49509975764888026 1104 118-119 0.5519160686147693 1104 120-121 0.475688301946807 1104 122-123 0.5222746244813337 1104 124-125 0.7202892575486857 1105 1 0.26379304103029 1105 2 0.29072336215214634 1105 3 0.304250290465216 1105 4 0.3309310144718509 1105 5 0.3718519209504052 1105 6 0.3201038886909586 1105 7 0.22989210279861538 1105 8 0.316274767045698 1105 9 0.2755976195608483 1105 10-11 0.3531677545901317 1105 12-13 0.41605647074829477 1105 14-15 0.4456166101340244 1105 16-17 0.4852194411232418 1105 18-19 0.47824318962995704 1105 20-21 0.4508130455056687 1105 22-23 0.3488475588586937 1105 24-25 0.45406996582632786 1105 26-27 0.46237058139017506 1105 28-29 0.5087241448282853 1105 30-31 0.570478410208608 1105 32-33 0.15389068620073232 1105 34-35 0.5237125351991736 1105 36-37 0.582791135167291 1105 38-39 0.5876476729821505 1105 40-41 0.5887451636031358 1105 42-43 0.5351112134163216 1105 44-45 0.5592447238467813 1105 46-47 0.5495349325465213 1105 48-49 0.5387401177478637 1105 50-51 0.5322478960019907 1105 52-53 0.5782424027800346 1105 54-55 0.6084504131951434 1105 56-57 0.6781716270936968 1105 58-59 0.668433045847479 1105 60-61 0.6084221559457674 1105 62-63 0.6763067383329258 1105 64-65 0.6415931227673113 1105 66-67 0.5680430258948519 1105 68-69 0.5898020897920446 1105 70-71 0.6589492990204491 1105 72-73 0.6396419492212502 1105 74-75 0.6167750977295512 1105 76-77 0.6770570566352205 1105 78-79 0.6186986780378234 1105 80-81 0.6273210175298018 1105 82-83 0.5607780458986973 1105 84-85 0.48720578225377054 1105 86-87 0.48918591309003645 1105 88-89 0.4859642658068424 1105 90-91 0.4251985554974098 1105 92-93 0.5183392731277436 1105 94-95 0.4949296185417964 1105 96-97 0.5105319518807647 1105 98-99 0.46277369534985624 1105 100-101 0.5185030677779991 1105 102-103 0.4861023794958257 1105 104-105 0.6185328594085817 1105 106-107 0.664221237924032 1105 108-109 0.613542878647042 1105 110-111 0.6241085228609862 1105 112-113 0.647474621048417 1105 114-115 0.6876727535106824 1105 116-117 0.7148121965065073 1105 118-119 0.7015825493487959 1105 120-121 0.7362904311400804 1105 122-123 0.6848783776567444 1105 124-125 0.6399511075076489 1106 1 0.33535176729653315 1106 2 0.409012431388188 1106 3 0.3982706360933719 1106 4 0.4813930268536879 1106 5 0.3358700566973596 1106 6 0.3284321965844086 1106 7 0.32326600254396 1106 8 0.25699799902519516 1106 9 0.40327868389167065 1106 10-11 0.3868999392417152 1106 12-13 0.30541066335167244 1106 14-15 0.4269684480413787 1106 16-17 0.3329488267060867 1106 18-19 0.39203903632000703 1106 20-21 0.4243037146203861 1106 22-23 0.41659381211723456 1106 24-25 0.5062493092399833 1106 26-27 0.47258327945996825 1106 28-29 0.5256095800336595 1106 30-31 0.4646464121901346 1106 32-33 0.4964347250590322 1106 34-35 0.6077978729027436 1106 36-37 0.5435151186091005 1106 38-39 0.5822186145336232 1106 40-41 0.5199488316293426 1106 42-43 0.560861207895897 1106 44-45 0.6141358362729008 1106 46-47 0.5210785610961253 1106 48-49 0.5097729145416707 1106 50-51 0.4870581363620943 1106 52-53 0.5993273397607624 1106 54-55 0.5096299950568692 1106 56-57 0.5305483962610751 1106 58-59 0.6162789936768291 1106 60-61 0.6413641497310536 1106 62-63 0.5682270720301261 1106 64-65 0.5327430364176138 1106 66-67 0.5444645692067311 1106 68-69 0.4662580480237537 1106 70-71 0.5251802773097012 1106 72-73 0.4214414720854762 1106 74-75 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74-75 -2.8303812464930296 2216 76-77 -2.881251655388265 2216 78-79 -2.9137649029745916 2216 80-81 -2.7750451952774995 2216 82-83 -2.5583532709542176 2216 84-85 -2.6374145645884255 2216 86-87 -2.829629498572203 2216 88-89 -2.617662174020932 2216 90-91 -2.6855711896212355 2216 92-93 -2.8958236311985743 2216 94-95 -2.8994962381540432 2216 96-97 -2.8176901735956292 2216 98-99 -2.8243383335606502 2216 100-101 -2.877433091231527 2216 102-103 -2.928712645453839 2216 104-105 -2.8030991494631863 2216 106-107 -2.79226128399889 2216 108-109 -2.7841802161778624 2216 110-111 -2.7048550917842817 2216 112-113 -2.681563133811835 2216 114-115 -2.8415055979990385 2216 116-117 -2.9952069311468428 2216 118-119 -3.1476006769670803 2216 120-121 -3.1267454418465945 2216 122-123 -3.14789980326087 2216 124-125 -3.43495048049396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61126.0 3 9645.0 4 5597.0 5 4564.0 6 4548.0 7 5434.0 8 6187.0 9 6949.0 10 7548.0 11 8264.0 12 8563.0 13 8736.0 14 8486.0 15 7691.0 16 6946.0 17 6360.0 18 6216.0 19 6052.0 20 6297.0 21 7077.0 22 8366.0 23 10989.0 24 14649.0 25 18676.0 26 23160.0 27 27199.0 28 32469.0 29 39689.0 30 47341.0 31 56514.0 32 71239.0 33 93323.0 34 127150.0 35 185109.0 36 339944.0 37 646515.0 38 302886.0 39 1354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39535191579779 18.37025369708474 12.671661180778527 24.562733206338947 2 15.708045507676225 19.2429895429265 40.210891420084494 24.838073529312783 3 19.304801893082477 23.38279014297726 29.052211422925716 28.260196541014544 4 12.738189938178632 15.318589347557769 36.84385741288858 35.09936330137502 5 13.83451298605147 37.533866218676835 34.73671174384889 13.894909051422808 6 33.818508180506775 35.64941878241302 16.79656194678147 13.735511090298733 7 29.579431951642622 31.624941187456677 20.499718058952617 18.295908801948087 8 26.23471132860188 36.17490136811749 19.26755360155099 18.322833701729643 9 25.95337722581158 16.01785094487966 19.791846739427662 38.2369250898811 10-11 25.321077432458377 26.22486591599835 27.762938811438094 20.69111784010518 12-13 25.32386046434335 23.46850369122106 28.052943208148758 23.154692636286832 14-15 22.480176165956664 25.46361884713499 25.10771260413715 26.948492382771192 16-17 22.870604811678565 28.655803922818812 25.805400060975526 22.668191204527098 18-19 22.70749868593203 27.872588508455955 27.215453484239156 22.204459321372862 20-21 23.323827321853223 27.156820898166522 27.146896505423722 22.37245527455653 22-23 23.083642471548803 27.05113580224735 26.943663489953384 22.921558236250462 24-25 22.89096000454471 27.198912503359697 26.975157464941862 22.934970027153735 26-27 22.842091080571276 27.352555465732507 26.931218000538937 22.87413545315728 28-29 22.7338978438858 27.405725570280488 27.078006808970745 22.78236977686297 30-31 22.91345829363913 27.244551388340987 26.9891314036581 22.852858914361786 32-33 22.73052430496149 27.26435645031571 27.076695716135184 22.92842352858761 34-35 23.04791105776409 27.36459657326223 26.800209346640454 22.787283022333227 36-37 23.097195673609676 27.16767208550092 26.857541404241204 22.877590836648203 38-39 22.810482941059814 27.396646270058984 26.947795272976244 22.845075515904956 40-41 23.026573031250315 27.210308420345402 26.943153611211123 22.819964937193156 42-43 22.948634561931065 27.130822201945147 26.87779004523303 23.042753190890753 44-45 22.85651717949903 27.352732246444937 26.88336823815394 22.907382335902096 46-47 22.81000413978332 27.356765180046917 26.794741914240294 23.038488765929472 48-49 22.88967013514872 27.292618165366882 26.924047065075328 22.893664634409067 50-51 22.801680269532394 27.357309019893627 26.83214626117143 23.008864449402544 52-53 22.928240945894917 27.337896892930765 26.749124559867944 22.984737601306378 54-55 22.886042711800677 27.032911611641232 26.85534025386759 23.225705422690503 56-57 22.737842861792497 27.354696540073903 26.978572266532318 22.92888833160128 58-59 22.92692174377348 27.20067663597949 26.851634773615235 23.020766846631798 60-61 23.01155496448889 27.162646680477167 27.019045298709273 22.806753056324673 62-63 22.86543712398573 27.224885954632256 26.996110868409673 22.913566052972335 64-65 22.717196144838265 27.11324189807076 26.900532178178942 23.26902977891203 66-67 22.71004117213579 27.347314386551336 26.796916443093682 23.145727998219197 68-69 22.575154240226066 27.138986309805397 27.02015872403185 23.265700725936682 70-71 22.545036169863263 27.11348423633072 27.059940810502948 23.28153878330307 72-73 22.707930758199065 27.03192995922068 26.959550597101682 23.30058868547857 74-75 22.561865035124477 27.101162617433154 26.934763446130205 23.402208901312164 76-77 22.65832783544946 27.1119054992303 26.96705308936193 23.262713575958315 78-79 22.615164708250752 27.10002206149524 27.00217046511153 23.282642765142477 80-81 22.483538742330964 27.17965094346609 27.049872881232318 23.286937432970632 82-83 22.562533255804716 27.205071646272017 27.090618940037782 23.141776157885488 84-85 22.647536357424254 27.012828467910875 26.942789723312178 23.396845451352693 86-87 22.514303178237224 26.810069814454167 27.271370733308697 23.40425627399991 88-89 22.62417119902735 26.79257417666322 27.21079230309897 23.37246232121046 90-91 22.488339512471807 27.00218446231623 27.25065134491619 23.258824680295774 92-93 22.510231097150548 26.809737491586265 27.291451649091318 23.388579762171865 94-95 22.406588268656773 26.758593179301343 27.227222308972156 23.60759624306973 96-97 22.418135455085682 26.77082952438018 27.222497526190082 23.588537494344056 98-99 22.407301512552277 26.556179712289907 27.22531310617018 23.811205668987633 100-101 22.245070215346196 26.660281686603472 27.360663619337384 23.733984478712948 102-103 22.353709590299182 26.52493445499946 27.359636835965183 23.76171911873617 104-105 22.25376405424059 26.508929103977273 27.413095899576785 23.824210942205347 106-107 22.22832128991694 26.495134872361316 27.46488663582153 23.811657201900218 108-109 22.19515920001597 26.543173469138324 27.240998766802292 24.02066856404342 110-111 22.141028079645128 26.61644582896682 27.341540576235662 23.900985515152385 112-113 22.149357100382872 26.631855601446425 27.40630049026942 23.812486807901283 114-115 22.02693731075474 26.73708646405742 27.30521475832679 23.930761466861053 116-117 21.92405918394764 26.630107504212543 27.471853399717272 23.973979912122545 118-119 21.946619351225607 26.562634622840648 27.34993143209983 24.140814593833916 120-121 22.000035897217295 26.66559722873483 27.36011864030315 23.97424823374473 122-123 22.028926934919667 26.660201477947528 27.336691373884747 23.974180213248058 124-125 21.932264513505444 26.64898135236355 27.250464469408048 24.168289664722955 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8919.0 1 4661.5 2 388.5 3 331.0 4 244.0 5 194.0 6 179.5 7 169.0 8 179.0 9 184.5 10 186.5 11 189.0 12 201.5 13 232.5 14 248.5 15 300.0 16 380.0 17 464.5 18 592.5 19 768.5 20 994.0 21 1428.5 22 2016.5 23 2880.5 24 4343.0 25 6429.5 26 9392.0 27 12700.5 28 15462.0 29 18280.0 30 22745.5 31 28184.0 32 32938.5 33 37158.5 34 42365.5 35 47155.0 36 52585.0 37 58547.0 38 61243.5 39 63516.5 40 66984.5 41 70689.5 42 74079.0 43 78637.5 44 83516.5 45 86477.5 46 87235.0 47 86892.0 48 87984.5 49 88639.5 50 87096.5 51 85071.5 52 82674.0 53 79721.5 54 76348.5 55 70994.5 56 64456.5 57 58589.0 58 53909.5 59 48029.0 60 39967.0 61 32139.0 62 25953.0 63 20492.0 64 15676.0 65 12025.0 66 9314.5 67 7590.0 68 5974.5 69 4347.0 70 3116.5 71 2358.5 72 1817.5 73 1398.0 74 1098.0 75 891.5 76 643.5 77 364.5 78 208.5 79 131.0 80 89.5 81 63.0 82 48.0 83 41.5 84 35.5 85 25.0 86 21.0 87 17.5 88 12.5 89 11.5 90 9.0 91 5.5 92 4.5 93 4.5 94 4.0 95 3.5 96 3.5 97 1.5 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4651031910018411 2 0.45661672156072425 3 0.45165883678196656 4 0.44741560206140807 5 0.4571973747330112 6 0.5159326763912673 7 0.5110641228697845 8 0.5197739204540887 9 0.5247764708614838 10-11 0.5438263614753593 12-13 0.5520225043303327 14-15 0.5300470150407038 16-17 0.5241511520605594 18-19 0.5357418826919795 20-21 0.5369478546651909 22-23 0.5466179632652004 24-25 0.5401191142984504 26-27 0.5475112758379496 28-29 0.5268757554074444 30-31 0.5331959418596446 32-33 0.5345135779044494 34-35 0.5328386168305449 36-37 0.480825492282226 38-39 0.4491575615782689 40-41 0.44667861918889 42-43 0.45949765460784026 44-45 0.46528185351639095 46-47 0.4681181209348695 48-49 0.48212079551271225 50-51 0.4647905316013789 52-53 0.4648575300443351 54-55 0.4545397698290825 56-57 0.4380581528618609 58-59 0.4549417604868196 60-61 0.4519714961824287 62-63 0.46758213339122 64-65 0.4676044662055387 66-67 0.47577827624619334 68-69 0.4841754144300353 70-71 0.4806021641390388 72-73 0.4919025681843154 74-75 0.48158480796906283 76-77 0.4791728640226401 78-79 0.4910762540545224 80-81 0.48343843155751726 82-83 0.4852250567030155 84-85 0.48263445024204304 86-87 0.47321000259953955 88-89 0.47856987803603446 90-91 0.4641652128004545 92-93 0.462646581426781 94-95 0.45692938096118646 96-97 0.4470359442179897 98-99 0.4101644677777689 100-101 0.40851183951818293 102-103 0.39759109331632464 104-105 0.4026606421666761 106-107 0.4023926483948513 108-109 0.4148766915990205 110-111 0.43638319178795615 112-113 0.4367181840027371 114-115 0.4291473599486882 116-117 0.42447980175607386 118-119 0.4649691941159288 120-121 0.4588500029925971 122-123 0.4235418235546872 124-125 0.43332359622629035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2238858.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.75246949607538 #Duplication Level Percentage of deduplicated Percentage of total 1 86.95313958663056 64.99961914537586 2 8.550843803223229 12.783933811322996 3 2.176038065615718 4.879926573667135 4 0.848361005078304 2.536683206151031 5 0.4132649704905736 1.5446288550196547 6 0.23776886562026142 1.0664285924637025 7 0.16055578673664664 0.8401359085314695 8 0.10677945327161197 0.6385622258795024 9 0.07679668233451596 0.5166667488249627 >10 0.42806351858056074 5.954972914332416 >50 0.033570388535506694 1.7240295245261963 >100 0.014696416314433542 1.6860214367642294 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.2145756800143533E-4 0.8283910571409292 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9124 0.4075291956881589 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.933125727491427E-5 0.0 0.0 0.0 0.0 3 8.933125727491427E-5 0.0 0.0 0.0 0.0 4 8.933125727491427E-5 0.0 0.0 0.0 0.0 5 8.933125727491427E-5 0.0 0.0 0.0 0.0 6 8.933125727491427E-5 0.0 0.0 0.0 0.0 7 8.933125727491427E-5 0.0 0.0 0.0 0.0 8 8.933125727491427E-5 0.0 0.0 0.0 0.0 9 8.933125727491427E-5 0.0 0.0 4.466562863745713E-5 0.0 10-11 8.933125727491427E-5 0.0 0.0 4.466562863745713E-5 0.0 12-13 8.933125727491427E-5 0.0 0.0 8.933125727491427E-5 0.0 14-15 8.933125727491427E-5 0.0 0.0 8.933125727491427E-5 0.0 16-17 8.933125727491427E-5 0.0 0.0 8.933125727491427E-5 0.0 18-19 1.5632970023109996E-4 0.0 0.0 1.1166407159364283E-4 0.0 20-21 2.2332814318728566E-4 2.2332814318728566E-5 0.0 1.339968859123714E-4 0.0 22-23 2.2332814318728566E-4 4.466562863745713E-5 0.0 1.5632970023109996E-4 0.0 24-25 2.679937718247428E-4 4.466562863745713E-5 0.0 2.2332814318728566E-4 0.0 26-27 2.679937718247428E-4 4.466562863745713E-5 0.0 2.9032658614347136E-4 0.0 28-29 3.349922147809285E-4 4.466562863745713E-5 0.0 0.0013176360448049853 0.0 30-31 3.5732502909965706E-4 4.466562863745713E-5 0.0 0.003841244062821313 0.0 32-33 3.7965784341838563E-4 4.466562863745713E-5 0.0 0.012260715060981982 0.0 34-35 4.4665628637457133E-4 4.466562863745713E-5 0.0 0.024543762936282694 0.0 36-37 5.359875436494856E-4 4.466562863745713E-5 0.0 0.04622892563976813 0.0 38-39 5.806531722869427E-4 4.466562863745713E-5 0.0 0.08251974890770206 0.0 40-41 5.806531722869427E-4 4.466562863745713E-5 0.0 0.12314313815346932 0.0 42-43 6.476516152431284E-4 4.466562863745713E-5 0.0 0.16405685398538006 0.0 44-45 7.146500581993141E-4 4.466562863745713E-5 0.0 0.21591364883346778 0.0 46-47 8.039813154742284E-4 4.466562863745713E-5 0.0 0.2806564775434619 0.0 48-49 8.039813154742284E-4 4.466562863745713E-5 0.0 0.35453342730981596 0.0 50-51 8.039813154742284E-4 4.466562863745713E-5 0.0 0.43263127898240983 0.0 52-53 8.039813154742284E-4 4.466562863745713E-5 0.0 0.5117117744850277 0.0 54-55 8.039813154742284E-4 4.466562863745713E-5 0.0 0.6007527051737984 0.0 56-57 8.039813154742284E-4 4.466562863745713E-5 0.0 0.7101834953355683 0.0 58-59 8.039813154742284E-4 4.466562863745713E-5 0.0 0.8323886552876512 0.0 60-61 8.039813154742284E-4 4.466562863745713E-5 0.0 0.9695121352046445 0.0 62-63 8.26314129792957E-4 4.466562863745713E-5 0.0 1.1030176992020038 0.0 64-65 8.486469441116855E-4 4.466562863745713E-5 0.0 1.2496549580187757 4.466562863745713E-5 66-67 8.486469441116855E-4 4.466562863745713E-5 0.0 1.4276698209533611 4.466562863745713E-5 68-69 8.486469441116855E-4 4.466562863745713E-5 0.0 1.6328413860995203 4.466562863745713E-5 70-71 8.486469441116855E-4 4.466562863745713E-5 0.0 1.8544275697699453 4.466562863745713E-5 72-73 8.486469441116855E-4 4.466562863745713E-5 0.0 2.076750736312888 4.466562863745713E-5 74-75 8.486469441116855E-4 4.466562863745713E-5 0.0 2.309681989657227 4.466562863745713E-5 76-77 8.486469441116855E-4 4.466562863745713E-5 0.0 2.5612388101433856 4.466562863745713E-5 78-79 8.486469441116855E-4 4.466562863745713E-5 0.0 2.8532850229893993 4.466562863745713E-5 80-81 8.933125727491427E-4 4.466562863745713E-5 0.0 3.1873839251975786 4.466562863745713E-5 82-83 8.933125727491427E-4 4.466562863745713E-5 0.0 3.5336095455808274 4.466562863745713E-5 84-85 8.933125727491427E-4 4.466562863745713E-5 0.0 3.8703213870642976 4.466562863745713E-5 86-87 8.933125727491427E-4 4.466562863745713E-5 0.0 4.22668610514825 4.466562863745713E-5 88-89 8.933125727491427E-4 4.466562863745713E-5 0.0 4.633455985149572 4.466562863745713E-5 90-91 9.379782013865998E-4 4.466562863745713E-5 0.0 5.0882190831218415 4.466562863745713E-5 92-93 9.82643830024057E-4 4.466562863745713E-5 0.0 5.562411729551405 4.466562863745713E-5 94-95 9.82643830024057E-4 8.933125727491427E-5 0.0 6.029770534799438 4.466562863745713E-5 96-97 9.82643830024057E-4 8.933125727491427E-5 0.0 6.506486789247018 4.466562863745713E-5 98-99 0.0010049766443427856 8.933125727491427E-5 0.0 7.027131689459537 4.466562863745713E-5 100-101 0.0010719750872989712 8.933125727491427E-5 0.0 7.612564084010687 4.466562863745713E-5 102-103 0.0011389735302551568 8.933125727491427E-5 0.0 8.228413771663947 4.466562863745713E-5 104-105 0.0014293001163986283 8.933125727491427E-5 0.0 8.826084548461761 4.466562863745713E-5 106-107 0.0015632970023109996 8.933125727491427E-5 0.0 9.41785946227943 4.466562863745713E-5 108-109 0.0016079626309484568 8.933125727491427E-5 0.0 10.04177129590175 4.466562863745713E-5 110-111 0.0016079626309484568 8.933125727491427E-5 0.0 10.736344154028526 4.466562863745713E-5 112-113 0.001652628259585914 8.933125727491427E-5 0.0 11.457738722152097 4.466562863745713E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2085 0.0 47.09714 1 GTATCAA 3180 0.0 44.728817 1 ATCAACG 3430 0.0 40.25775 3 TCAACGC 3540 0.0 39.00856 4 CAACGCA 3565 0.0 38.737617 5 AACGCAG 3820 0.0 35.996708 6 GTACATG 7350 0.0 34.655575 1 TATCAAC 4205 0.0 33.542778 2 TACATGG 7605 0.0 33.02402 2 ACATGGG 7895 0.0 30.909174 3 ACGCAGA 4645 0.0 29.093325 7 CGCAGAG 4865 0.0 27.410591 8 GAGTACA 5710 0.0 26.682133 1 AGTACAT 5415 0.0 26.377247 2 CATGGGG 5135 0.0 24.341875 4 GCAGAGT 5885 0.0 21.445818 9 AGAGTAC 4910 0.0 20.006462 10-11 CAGAGTA 5400 0.0 19.458921 10-11 CATGGGA 3650 0.0 18.753408 4 TAAGACG 230 1.4329422E-4 18.115295 4 >>END_MODULE