FastQCFastQC Report
Thu 26 May 2016
SRR1364092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238858
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.14623526815903465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31950.14270668349667553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.063.9701841
GTATCAA52250.050.6417661
ATCAACG65450.039.723383
CAACGCA68200.038.0352485
TCAACGC69100.037.5398524
AACGCAG70600.037.079356
TATCAAC73050.035.8349572
GTACATG72950.035.536621
TACATGG75350.033.8725932
ACATGGG79050.030.9324513
ACGCAGA84950.030.6097937
CGCAGAG87050.029.4612268
GTGGTAT17950.027.8886181
CATGGGG50800.026.936944
TACCGTA1401.0559389E-425.501497
GCAGAGT100600.025.1972969
TGGTATC18300.025.0330682
GAGTACT63250.023.98954412-13
AGAGTAC89500.023.96759810-11
GAGTACA55800.023.7099651
Produced by FastQC (version 0.11.5)