FastQCFastQC Report
Thu 26 May 2016
SRR1364091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940669
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78730.4056848437317235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.050.4437331
GTATCAA38550.048.0123251
GTACATG106950.039.3419041
TCAACGC46050.039.1669434
ATCAACG46150.038.9480483
CAACGCA47400.037.927815
AACGCAG47900.037.4085966
TACATGG112950.036.988582
ACATGGG113350.034.7080653
TATCAAC52700.033.8787462
CATGGGA68450.030.0021484
ACGCAGA59900.029.9151617
CGCAGAG61150.029.0115878
AGTACAT75350.028.670822
GAGTACA79900.028.3043731
CATGGGG47200.027.7451674
GTGGTAT16750.024.8713841
GCAGAGT69500.024.6694419
ATGGGAG32050.024.5174355
ATGGGGG25950.023.3987145
Produced by FastQC (version 0.11.5)