FastQCFastQC Report
Thu 26 May 2016
SRR1364091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940669
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56530.2912913021231338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55890.2879934702929763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.17112655481176853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.074.7781141
GTATCAA70950.052.827851
ATCAACG93100.039.8754233
TCAACGC94950.039.0984954
CAACGCA96600.038.4922525
TATCAAC98550.037.852322
AACGCAG99100.037.7623146
GTACATG100850.036.4564171
TACATGG105950.034.4223982
ACATGGG103650.033.6929633
TGGTATC19150.032.9320452
GTGGTAT19650.032.7511021
ACGCAGA119500.031.3263557
CGCAGAG121050.030.7777358
CATGGGA61150.030.16034
GCAGAGT133300.027.6367879
CATGGGG46400.027.0542134
TCTAGCG900.00953433126.4422912
GAGTACT83250.025.52211412-13
AGTACAT73450.025.1102772
Produced by FastQC (version 0.11.5)