FastQCFastQC Report
Thu 26 May 2016
SRR1364090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207801
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131490.4099069736557847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.046.5022051
GTATCAA44900.042.8113481
ATCAACG49350.036.906113
TCAACGC50150.036.206074
CAACGCA50300.036.0986675
AACGCAG53100.034.3072786
TACATGG97000.032.9477422
TATCAAC56750.032.9296342
GTACATG99300.032.8423351
ACATGGG99500.030.9265463
CATGGGG63650.026.5627144
CGCAGAG67700.026.4706388
GTACCGT3450.025.8840056
ACGCAGA71550.025.2127117
GCAGAGT79850.021.4735629
GAGTACA75400.020.994861
AGTACAT73650.020.2018152
AGAGTAC69300.019.71724310-11
ATGGGCG4304.9112714E-1119.3829065
CATGGGA43100.019.337634
Produced by FastQC (version 0.11.5)