##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364090_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3207801 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.089228415353695 31.0 31.0 34.0 28.0 34.0 2 31.179601228380438 33.0 31.0 34.0 28.0 34.0 3 31.237343588333566 33.0 31.0 34.0 28.0 34.0 4 34.74725053081535 37.0 35.0 37.0 32.0 37.0 5 34.78402494419074 37.0 35.0 37.0 32.0 37.0 6 34.83783750924699 37.0 35.0 37.0 32.0 37.0 7 34.734784670246064 37.0 35.0 37.0 32.0 37.0 8 34.69762962228642 37.0 35.0 37.0 32.0 37.0 9 36.26212442729459 39.0 37.0 39.0 32.0 39.0 10-11 36.1762953811661 39.0 37.0 39.0 32.0 39.0 12-13 36.09015116586097 39.0 37.0 39.0 31.0 39.0 14-15 37.37309203407568 40.0 38.0 41.0 31.0 41.0 16-17 37.373082370134554 40.0 38.0 41.0 31.0 41.0 18-19 37.37107102342072 40.0 38.0 41.0 31.0 41.0 20-21 37.39005552401785 40.0 38.0 41.0 31.0 41.0 22-23 37.34133757050391 40.0 38.0 41.0 31.0 41.0 24-25 37.34599559012545 40.0 38.0 41.0 31.0 41.0 26-27 37.304312985749426 40.0 38.0 41.0 31.0 41.0 28-29 37.262231198257 40.0 37.0 41.0 31.0 41.0 30-31 37.21860598584513 40.0 37.0 41.0 31.0 41.0 32-33 37.130490326550806 40.0 37.0 41.0 30.5 41.0 34-35 37.108621139528296 40.0 37.0 41.0 30.0 41.0 36-37 37.05367929619075 40.0 37.0 41.0 30.0 41.0 38-39 36.98262968931053 40.0 37.0 41.0 30.0 41.0 40-41 36.9116084507736 40.0 37.0 41.0 30.0 41.0 42-43 36.81220000866637 40.0 37.0 41.0 30.0 41.0 44-45 36.7293273491716 40.0 37.0 41.0 30.0 41.0 46-47 36.62957209627405 40.0 36.0 41.0 30.0 41.0 48-49 36.53285069740922 40.0 36.0 41.0 30.0 41.0 50-51 36.42029399579338 40.0 36.0 41.0 30.0 41.0 52-53 36.288352519373866 40.0 35.5 41.0 29.0 41.0 54-55 36.12585833722229 39.5 35.0 41.0 29.0 41.0 56-57 35.954383548106634 39.0 35.0 41.0 28.5 41.0 58-59 35.75682936690899 39.0 35.0 41.0 28.0 41.0 60-61 35.532181391551404 39.0 35.0 41.0 28.0 41.0 62-63 35.28112825577397 38.0 34.5 40.0 28.0 41.0 64-65 35.01036831773543 38.0 34.0 40.0 28.0 41.0 66-67 34.705850830522216 37.0 34.0 40.0 27.5 41.0 68-69 34.39177664075795 37.0 34.0 39.5 27.0 41.0 70-71 34.04984723179524 36.0 34.0 39.0 26.0 41.0 72-73 33.70251988823496 36.0 33.5 39.0 26.0 40.5 74-75 33.423434776658524 35.0 33.0 38.0 26.0 40.0 76-77 33.18599828979416 35.0 33.0 37.0 26.0 39.5 78-79 32.884165040163026 35.0 33.0 37.0 26.0 39.0 80-81 32.66493011879477 35.0 33.0 36.5 26.0 39.0 82-83 32.55006716439081 35.0 33.0 36.0 26.0 37.5 84-85 32.32643374698119 35.0 33.0 36.0 26.0 37.0 86-87 32.13293218625469 35.0 33.0 35.0 26.0 37.0 88-89 31.95329479603005 35.0 33.0 35.0 26.0 36.0 90-91 31.80873875904397 35.0 33.0 35.0 25.5 36.0 92-93 31.687295284214947 35.0 33.0 35.0 25.0 36.0 94-95 31.59016332372239 35.0 33.0 35.0 25.0 36.0 96-97 31.509778661456867 35.0 33.0 35.0 25.0 35.5 98-99 31.437443282797155 35.0 33.0 35.0 25.0 35.0 100-101 31.365513010314544 35.0 33.0 35.0 24.5 35.0 102-103 30.28186006550905 34.0 30.5 35.0 21.5 35.0 104-105 31.101752571309753 35.0 32.5 35.0 24.0 35.0 106-107 31.19314477425501 35.0 33.0 35.0 24.0 35.0 108-109 31.173930988861215 35.0 33.0 35.0 24.0 35.0 110-111 31.10820247265962 35.0 33.0 35.0 23.0 35.0 112-113 31.03498346686718 35.0 33.0 35.0 23.0 35.0 114-115 30.93943763967902 35.0 33.0 35.0 20.0 35.0 116-117 30.84555401036411 35.0 33.0 35.0 19.0 35.0 118-119 30.713484876399754 35.0 33.0 35.0 18.0 35.0 120-121 30.579996078310344 35.0 33.0 35.0 11.0 35.0 122-123 30.41496199421348 35.0 33.0 35.0 2.0 35.0 124-125 29.60732882120805 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4602355341414146 1101 2 0.520066287645033 1101 3 0.5303249605502742 1101 4 0.6240441768698659 1101 5 0.6108452074536999 1101 6 0.5274328117306979 1101 7 0.5630725492313715 1101 8 0.519896675364862 1101 9 0.5465086232834651 1101 10-11 0.5951597412441814 1101 12-13 0.7054851765467376 1101 14-15 0.7490462363074144 1101 16-17 0.688348869368042 1101 18-19 0.7532043877272514 1101 20-21 0.7990106427372154 1101 22-23 0.7511725274161165 1101 24-25 0.6726906644475577 1101 26-27 0.7035316122246087 1101 28-29 0.8404296481600468 1101 30-31 0.8004208350016597 1101 32-33 0.773740392485827 1101 34-35 0.8949503557865626 1101 36-37 0.8414959112103162 1101 38-39 0.7389853551971513 1101 40-41 0.8039098035640109 1101 42-43 0.8124421964522668 1101 44-45 0.8101132609008275 1101 46-47 0.7856259924634017 1101 48-49 0.7452789439978744 1101 50-51 0.8300945227726828 1101 52-53 0.7995605421155005 1101 54-55 0.8101022740101129 1101 56-57 0.7435473053932355 1101 58-59 0.7853089215570535 1101 60-61 0.7639821079203983 1101 62-63 0.8121071136696614 1101 64-65 0.8559693284461005 1101 66-67 0.8628482745298527 1101 68-69 0.8234614856359883 1101 70-71 0.7768654495644043 1101 72-73 0.7460257161674875 1101 74-75 0.7987001997346255 1101 76-77 0.7769736031606342 1101 78-79 0.7385630945448085 1101 80-81 0.715856825878987 1101 82-83 0.7618261726593332 1101 84-85 0.7370470293138212 1101 86-87 0.7041891870533235 1101 88-89 0.7455928821186824 1101 90-91 0.7537283795828138 1101 92-93 0.7437317010849291 1101 94-95 0.7797560369490846 1101 96-97 0.7470792491688449 1101 98-99 0.7303014932425675 1101 100-101 0.7807304787944958 1101 102-103 0.8422849600614875 1101 104-105 0.8080055019699444 1101 106-107 0.8090586933036441 1101 108-109 0.8870974677865711 1101 110-111 0.8170091375281814 1101 112-113 0.8116436919241856 1101 114-115 0.8967540607306326 1101 116-117 0.8767876417312372 1101 118-119 0.8305303938252884 1101 120-121 0.8097836685605806 1101 122-123 0.8866466322521696 1101 124-125 0.9766017679707986 1102 1 0.3089643583037649 1102 2 0.30475757847794327 1102 3 0.35480131645726587 1102 4 0.3593154486295731 1102 5 0.3506143897806808 1102 6 0.3580789371745112 1102 7 0.33248095339411776 1102 8 0.2876903914967315 1102 9 0.30181509426913067 1102 10-11 0.31402034702674797 1102 12-13 0.3463594088226998 1102 14-15 0.38974304752445477 1102 16-17 0.45275672788717003 1102 18-19 0.45403112468115125 1102 20-21 0.5169644201201891 1102 22-23 0.5002093100511615 1102 24-25 0.4951331946212889 1102 26-27 0.5064460094309169 1102 28-29 0.5095347683128466 1102 30-31 0.5234195567359308 1102 32-33 0.4981169512193233 1102 34-35 0.49518117693502006 1102 36-37 0.4068866928221979 1102 38-39 0.4637438644571077 1102 40-41 0.4617077632044584 1102 42-43 0.421594638155284 1102 44-45 0.4327119077046717 1102 46-47 0.4704922194177783 1102 48-49 0.49579704476886377 1102 50-51 0.46733040693775507 1102 52-53 0.46872283320698216 1102 54-55 0.419273656844787 1102 56-57 0.4866749762665705 1102 58-59 0.4749346725989838 1102 60-61 0.4680468226426271 1102 62-63 0.49751377107776307 1102 64-65 0.529696779810827 1102 66-67 0.4808147720122449 1102 68-69 0.4595956562716097 1102 70-71 0.47781174155115735 1102 72-73 0.48757180254738586 1102 74-75 0.48064792846703597 1102 76-77 0.4824330107515422 1102 78-79 0.4193641897245186 1102 80-81 0.44398181719039087 1102 82-83 0.4258705352224794 1102 84-85 0.46027834466941187 1102 86-87 0.418574907038753 1102 88-89 0.44817071800576613 1102 90-91 0.5129978044953027 1102 92-93 0.5304485634882035 1102 94-95 0.5083564680422832 1102 96-97 0.5398572737026548 1102 98-99 0.5553998654868906 1102 100-101 0.530392840089803 1102 102-103 0.5614236876901018 1102 104-105 0.552450884782381 1102 106-107 0.527636050970596 1102 108-109 0.5346097847353093 1102 110-111 0.6009656340930576 1102 112-113 0.5779609555319105 1102 114-115 0.614482803567757 1102 116-117 0.6123357320481126 1102 118-119 0.5996083708151154 1102 120-121 0.5982304297313341 1102 122-123 0.6168629677791984 1102 124-125 0.7388194060916149 1103 1 0.2888547958601073 1103 2 0.34733485775430495 1103 3 0.32335941845358107 1103 4 0.31975364061811007 1103 5 0.4009908662155084 1103 6 0.35143160958897113 1103 7 0.39509845179536285 1103 8 0.4095290103319158 1103 9 0.39307523572949776 1103 10-11 0.35835143440703376 1103 12-13 0.4085078767205985 1103 14-15 0.5316252563402983 1103 16-17 0.46687892815067755 1103 18-19 0.47984321199209035 1103 20-21 0.4006999928574686 1103 22-23 0.5239589151794846 1103 24-25 0.537075789910574 1103 26-27 0.5277002181851245 1103 28-29 0.49875115991768837 1103 30-31 0.42047000977893845 1103 32-33 0.251267015939753 1103 34-35 0.5040857740118128 1103 36-37 0.49530640674248616 1103 38-39 0.5685589393093622 1103 40-41 0.6528340816906137 1103 42-43 0.6409624678635026 1103 44-45 0.613678226113791 1103 46-47 0.6648791185386642 1103 48-49 0.6399606006172078 1103 50-51 0.6417863989709787 1103 52-53 0.5970135107682637 1103 54-55 0.663751954312211 1103 56-57 0.6513836572796023 1103 58-59 0.6323950505971183 1103 60-61 0.6679219565137586 1103 62-63 0.7135290439437227 1103 64-65 0.6852783872660027 1103 66-67 0.6916890082726397 1103 68-69 0.6925273280619777 1103 70-71 0.6735823769671043 1103 72-73 0.6235822213810351 1103 74-75 0.6762921567914688 1103 76-77 0.6252828959498302 1103 78-79 0.6464650033527946 1103 80-81 0.6184758517387721 1103 82-83 0.5826025675981086 1103 84-85 0.47530899056391007 1103 86-87 0.47616044009625824 1103 88-89 0.4686621502534898 1103 90-91 0.43559356569232577 1103 92-93 0.4100249817517003 1103 94-95 0.4484874209735814 1103 96-97 0.4407673566064396 1103 98-99 0.4253659739398401 1103 100-101 0.4385384649116304 1103 102-103 0.44377383870443765 1103 104-105 0.47566038250560894 1103 106-107 0.4927212084184518 1103 108-109 0.49173587015086895 1103 110-111 0.49448439338699046 1103 112-113 0.482265490199719 1103 114-115 0.5312079786596371 1103 116-117 0.520779436830491 1103 118-119 0.5939932474225316 1103 120-121 0.6200295132438036 1103 122-123 0.5961849345868622 1103 124-125 0.5943120229543695 1104 1 0.2594241497004184 1104 2 0.2671523167637204 1104 3 0.2836540837774457 1104 4 0.27421109552624046 1104 5 0.26188725077350483 1104 6 0.2673265387846371 1104 7 0.26616495909950544 1104 8 0.36260304484624584 1104 9 0.3199251664779794 1104 10-11 0.30661885416191836 1104 12-13 0.3442621463130209 1104 14-15 0.41261432650571095 1104 16-17 0.38499111349477033 1104 18-19 0.46419196595196865 1104 20-21 0.3760422572148201 1104 22-23 0.35464170254363125 1104 24-25 0.46858127008057693 1104 26-27 0.45992573197886344 1104 28-29 0.4953291942422027 1104 30-31 0.5018187472150544 1104 32-33 -0.24274032315373262 1104 34-35 0.2925227614838306 1104 36-37 0.4514054976388664 1104 38-39 0.5026410251337907 1104 40-41 0.53454274060919 1104 42-43 0.5665980925910219 1104 44-45 0.5553429191085968 1104 46-47 0.4777650066931116 1104 48-49 0.5231867342639518 1104 50-51 0.505387702568882 1104 52-53 0.4711077224936062 1104 54-55 0.45587700361870276 1104 56-57 0.44901614079714136 1104 58-59 0.5406469549609696 1104 60-61 0.5208309864120295 1104 62-63 0.5742862051949018 1104 64-65 0.5277051647464006 1104 66-67 0.535820317967385 1104 68-69 0.5685180866171535 1104 70-71 0.5348788484655174 1104 72-73 0.5000999609131256 1104 74-75 0.49419158807739194 1104 76-77 0.5035063116491827 1104 78-79 0.4052924643238711 1104 80-81 0.3972229436621717 1104 82-83 0.3859239289698664 1104 84-85 0.36131098951570806 1104 86-87 0.4146221496618665 1104 88-89 0.4314936794600861 1104 90-91 0.39707118425350885 1104 92-93 0.3959663088302925 1104 94-95 0.3923912746967453 1104 96-97 0.39162773630559755 1104 98-99 0.3668124896922862 1104 100-101 0.40503291017284937 1104 102-103 0.390047030761 1104 104-105 0.4460248628522834 1104 106-107 0.37790254825847924 1104 108-109 0.42451537492094005 1104 110-111 0.4401078625555499 1104 112-113 0.4155474021433072 1104 114-115 0.4949837138930029 1104 116-117 0.49017111232748434 1104 118-119 0.5320922815449549 1104 120-121 0.456664181711421 1104 122-123 0.562454190598018 1104 124-125 0.6690111315608362 1105 1 0.3064622732328708 1105 2 0.32509435603948944 1105 3 0.3172047853974327 1105 4 0.3555657961413914 1105 5 0.3332793420557252 1105 6 0.28372839814507245 1105 7 0.18338023674752435 1105 8 0.2492945832614737 1105 9 0.2982832158519244 1105 10-11 0.4309538255427938 1105 12-13 0.4106440750569149 1105 14-15 0.47068549956782846 1105 16-17 0.4743227319646408 1105 18-19 0.4648217526736147 1105 20-21 0.45437850747693176 1105 22-23 0.37939064943891765 1105 24-25 0.5216888891815756 1105 26-27 0.5516182980665008 1105 28-29 0.4788535293173908 1105 30-31 0.4693044376552109 1105 32-33 0.13948302016566316 1105 34-35 0.4561044386036883 1105 36-37 0.4480122063674372 1105 38-39 0.4770139892524625 1105 40-41 0.5143733718907058 1105 42-43 0.4886715618262514 1105 44-45 0.431950084580464 1105 46-47 0.43380211500978305 1105 48-49 0.3980363722948894 1105 50-51 0.4746024777992801 1105 52-53 0.46431387358013865 1105 54-55 0.45338059009163345 1105 56-57 0.4236000372742055 1105 58-59 0.4473414622076106 1105 60-61 0.3306373687767561 1105 62-63 0.3463895703549369 1105 64-65 0.43046690460618464 1105 66-67 0.464980782267709 1105 68-69 0.4665032258511914 1105 70-71 0.4520611160298884 1105 72-73 0.3621495756916744 1105 74-75 0.40447391771733976 1105 76-77 0.4418897725592288 1105 78-79 0.48343262638469753 1105 80-81 0.46351217427807967 1105 82-83 0.43200662857187666 1105 84-85 0.416723916596645 1105 86-87 0.370399537131739 1105 88-89 0.3561175409755144 1105 90-91 0.38022763336504184 1105 92-93 0.4322090969574539 1105 94-95 0.4033524909626216 1105 96-97 0.37549712017965575 1105 98-99 0.40440308576304673 1105 100-101 0.35972013444003537 1105 102-103 0.3710240877148152 1105 104-105 0.4651014732357197 1105 106-107 0.4811015160186294 1105 108-109 0.436915332568379 1105 110-111 0.386609727936694 1105 112-113 0.4044562216487364 1105 114-115 0.4172101921323019 1105 116-117 0.43513250713846574 1105 118-119 0.5131464658325697 1105 120-121 0.522625179986175 1105 122-123 0.5042002164123183 1105 124-125 0.5285849900659159 1106 1 0.11486099633813396 1106 2 0.21789536993352243 1106 3 0.18873922150349287 1106 4 0.19412866068454093 1106 5 0.05202967503743139 1106 6 0.07380264967010675 1106 7 0.1621751678623582 1106 8 0.07102205996110911 1106 9 0.22885048714024236 1106 10-11 0.2668875187784252 1106 12-13 0.239843379153875 1106 14-15 0.3142954996952696 1106 16-17 0.22035980570365865 1106 18-19 0.2904580950751239 1106 20-21 0.2920462963050028 1106 22-23 0.2790196947618355 1106 24-25 0.3838827262151625 1106 26-27 0.31148601631812767 1106 28-29 0.30858161358459313 1106 30-31 0.3221317892268587 1106 32-33 0.26426809940956275 1106 34-35 0.3334291732264063 1106 36-37 0.34413077313478624 1106 38-39 0.3950895210986758 1106 40-41 0.3958522974655594 1106 42-43 0.34738847441361287 1106 44-45 0.37401488245456704 1106 46-47 0.3659069417245391 1106 48-49 0.37251084620468333 1106 50-51 0.3047093933931393 1106 52-53 0.25011822888681934 1106 54-55 0.25657008995363384 1106 56-57 0.33226493681348757 1106 58-59 0.46773993710439754 1106 60-61 0.39726507005492095 1106 62-63 0.3472592402188539 1106 64-65 0.364205937625222 1106 66-67 0.3420098813691226 1106 68-69 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-2.0681281414459924 2216 98-99 -2.18762171643046 2216 100-101 -2.144470809465595 2216 102-103 -2.2422232089865837 2216 104-105 -2.091522410046526 2216 106-107 -2.0985194407213115 2216 108-109 -2.2154365319306173 2216 110-111 -2.1183234821985337 2216 112-113 -2.027742721206277 2216 114-115 -2.152911189951208 2216 116-117 -2.2372153212365156 2216 118-119 -2.4344248000655426 2216 120-121 -2.4481712309175627 2216 122-123 -2.5495351834728943 2216 124-125 -2.838788726591133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57483.0 3 9729.0 4 5429.0 5 4422.0 6 4903.0 7 5620.0 8 6698.0 9 7613.0 10 8203.0 11 9071.0 12 9871.0 13 9651.0 14 9869.0 15 8526.0 16 7995.0 17 7672.0 18 7206.0 19 7271.0 20 7483.0 21 8442.0 22 10018.0 23 12924.0 24 17245.0 25 22766.0 26 28552.0 27 33721.0 28 40784.0 29 51147.0 30 60324.0 31 73980.0 32 95392.0 33 125024.0 34 173380.0 35 258160.0 36 482784.0 37 1000515.0 38 514795.0 39 3133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55490686770422 18.858890555849623 12.9279277868434 24.658274789602757 2 15.540965452272323 19.826985761495656 40.34356686685257 24.28848191937945 3 18.713594604548152 24.343387590160116 29.690925238819393 27.252092566472335 4 12.458867105835077 15.907798433474001 37.681238389782635 33.95209607090829 5 13.40759517774049 37.860856754689756 35.274501366415436 13.45704670115433 6 32.84090207581977 36.338236754948724 17.35267877987185 13.468182389359647 7 28.35348256570722 32.066013359846636 21.75220925036834 17.828294824077805 8 25.621302146008823 36.25043872843963 20.04744534697152 18.080813778580023 9 25.910332806203705 16.01336439700069 20.24199700018741 37.83430579660819 10-11 24.733068755620657 26.26408161640453 28.572700306967402 20.43014932100741 12-13 24.90259291408709 23.730337938088937 28.589297117239294 22.777772030584682 14-15 22.25692925260979 25.71430004174664 25.57384391270063 26.45492679294294 16-17 22.5026301628993 28.82970365107665 26.308743046952443 22.358923139071607 18-19 22.366035286377812 28.07748134083346 27.515488839917467 22.040994532871256 20-21 22.967324647425233 27.327026367550978 27.545158766190873 22.16049021883292 22-23 22.826545052232756 27.1859228513398 27.21786450750684 22.7696675889206 24-25 22.61806661962944 27.478755672128187 27.328710896911684 22.574466811330684 26-27 22.589203317233668 27.645489459984052 27.24190063846968 22.523406584312607 28-29 22.50145026280235 27.708261969329957 27.318234512274504 22.47205325559319 30-31 22.55100428945651 27.51683531759091 27.334064853974816 22.598095538977763 32-33 22.44153663184114 27.491099814810955 27.47893880110897 22.588424752238936 34-35 22.78453856332715 27.564296240670316 27.121655275993206 22.52950992000933 36-37 22.9532106724982 27.28748580372418 27.15320951345872 22.6060940103189 38-39 22.468445402545413 27.588313102775324 27.23229244236624 22.710949052313023 40-41 22.833914217090822 27.56816381235106 27.182009200196656 22.415912770361462 42-43 22.57469367992635 27.48403590388987 27.114073192986776 22.827197223197004 44-45 22.55855663422616 27.545949639986965 27.299744853416385 22.59574887237049 46-47 22.688272860202712 27.631900195647823 26.920956116974004 22.758870827175457 48-49 22.588724636650593 27.537274708331367 27.15944113272072 22.714559522297318 50-51 22.585043601362106 27.62812818338489 27.06610635177534 22.720721863477664 52-53 22.636533105825137 27.510608070778105 27.078987688749745 22.773871134647017 54-55 22.688925888073065 27.329292721778884 27.159054022758312 22.822727367389735 56-57 22.54170339278921 27.73352725769761 27.06343925590658 22.6613300936066 58-59 22.599801074299002 27.487636041128017 27.22238089511555 22.69018198945744 60-61 22.77061671245566 27.5170037243942 27.107408046482746 22.60497151666739 62-63 22.42795469946039 27.586210136635465 27.27940714718948 22.706428016714664 64-65 22.58240367732224 27.44493945360093 27.20264110214429 22.770015766932538 66-67 22.579853491843508 27.565353184327673 26.983195242251533 22.87159808157729 68-69 22.30261205163487 27.488763706163965 27.27919184831258 22.929432393888586 70-71 22.471309834057436 27.258833216203975 27.389380108224703 22.88047684151388 72-73 22.502754571084225 27.277146035657633 27.25353990188495 22.966559491373186 74-75 22.365264804460875 27.66591187923276 26.979758571395234 22.989064744911136 76-77 22.50873487880806 27.491750653275588 27.0777640715029 22.921750396413454 78-79 22.60101613328937 27.332748016331443 27.19252256224737 22.87371328813182 80-81 22.428015703158678 27.594883692372463 27.0733507423038 22.90374986216506 82-83 22.603157915511364 27.603394431811097 27.00427201692642 22.789175635751118 84-85 22.652918212853457 27.408276638947555 27.033432719494826 22.90537242870416 86-87 22.448879551272363 27.203478688491213 27.376254176673587 22.971387583562837 88-89 22.564231796422902 27.246350889700672 27.250156777518765 22.93926053635766 90-91 22.692163622833633 27.325156033504292 27.117977865917673 22.864702477744398 92-93 22.45329847275659 27.16171567030008 27.24507224523589 23.139913611707442 94-95 22.445560194771925 27.2445340969876 27.28366574091125 23.026239967329225 96-97 22.39351311674899 27.18521148945418 27.26410863913612 23.15716675466071 98-99 22.45986919425672 27.05902755115728 27.218250135502753 23.26285311908325 100-101 22.3336544413464 27.083084546357327 27.35403606165587 23.229224950640404 102-103 22.418754230226014 26.89099025434173 27.40153598773088 23.288719527701378 104-105 22.441591465309823 27.01628894097939 27.227487091011927 23.31463250269886 106-107 22.36812596668447 27.02267208137779 27.223697066224627 23.38550488571311 108-109 22.415470838787414 27.11009275547512 27.078319141379904 23.396117264357567 110-111 22.307596929516397 27.140139346551596 27.256428464971112 23.2958352589609 112-113 22.48490529084683 27.184002888139542 27.334656437734743 22.996435383278882 114-115 22.31091014915289 27.30618092650184 27.006992236310225 23.37591668803505 116-117 22.214788538930073 27.232634335779615 27.219798448391668 23.332778676898645 118-119 22.419855206142543 27.201168874488502 27.07739095437725 23.301584964991708 120-121 22.494661062413346 27.30628167229399 27.00623948693585 23.192817778356815 122-123 22.347363897991883 27.45019617849151 27.051374266923283 23.151065656593328 124-125 22.335419222366042 27.517829623381495 26.95282045887054 23.193930695381926 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13054.0 1 6872.5 2 696.0 3 627.0 4 474.5 5 370.0 6 321.0 7 314.0 8 333.0 9 328.0 10 325.0 11 317.0 12 326.0 13 347.5 14 383.0 15 441.5 16 509.5 17 627.0 18 807.0 19 1077.0 20 1459.5 21 2069.5 22 3019.0 23 4378.0 24 6308.0 25 9504.5 26 14616.0 27 20287.5 28 24794.5 29 29679.0 30 36461.5 31 44063.5 32 51587.5 33 57929.0 34 65071.5 35 73551.5 36 82681.5 37 90927.5 38 95368.5 39 98287.5 40 101268.5 41 106584.5 42 111958.5 43 115035.5 44 118428.5 45 120503.5 46 122187.5 47 125303.0 48 124380.0 49 122193.0 50 120717.0 51 114824.0 52 110119.0 53 110864.0 54 110223.0 55 101353.5 56 88305.0 57 77247.0 58 69452.5 59 61582.5 60 51554.5 61 42283.0 62 34732.0 63 27456.0 64 21568.5 65 16808.5 66 12164.0 67 9859.0 68 7547.5 69 4699.0 70 3330.5 71 2464.0 72 1908.5 73 1452.0 74 1113.5 75 908.5 76 573.0 77 273.5 78 150.0 79 91.5 80 48.5 81 33.0 82 26.5 83 17.0 84 11.0 85 12.0 86 16.0 87 15.5 88 13.5 89 8.5 90 6.0 91 7.5 92 8.0 93 6.0 94 8.5 95 9.0 96 5.5 97 3.0 98 3.5 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46857644847669794 2 0.460408859527134 3 0.45492223488925904 4 0.45180483452683 5 0.46059590354887975 6 0.5183613322646885 7 0.5127188376086921 8 0.522507474746719 9 0.5279317513773454 10-11 0.5474155036425264 12-13 0.5560818766500789 14-15 0.5338548120659604 16-17 0.5279005773737211 18-19 0.5386244346204768 20-21 0.5395440677273933 22-23 0.5489586168219288 24-25 0.5430511431351259 26-27 0.5509381660520711 28-29 0.5299580616129242 30-31 0.5357876002906664 32-33 0.5386088476186647 34-35 0.5355382082616721 36-37 0.4832749911855505 38-39 0.4503708303601127 40-41 0.44862508615715246 42-43 0.4606426645543162 44-45 0.4654434611124568 46-47 0.46993251763435445 48-49 0.4838984712580363 50-51 0.4661604631958155 52-53 0.4660201801795062 54-55 0.4563094780505399 56-57 0.44005223516047287 58-59 0.4566679790922193 60-61 0.45295827266092875 62-63 0.46871673149300724 64-65 0.4689505365201894 66-67 0.47641671038820677 68-69 0.48564421546099645 70-71 0.4819968570369546 72-73 0.4936091733870025 74-75 0.4835555572181691 76-77 0.4809213539119166 78-79 0.4925024962583402 80-81 0.48559745445556 82-83 0.487312024654896 84-85 0.48441284231783704 86-87 0.4744215741562522 88-89 0.47942500173795066 90-91 0.46439913199104305 92-93 0.4642276749711095 94-95 0.45928659539665956 96-97 0.448999174200644 98-99 0.4120112189004243 100-101 0.4094237765996083 102-103 0.39866874534922836 104-105 0.40320456287656253 106-107 0.4029084098421318 108-109 0.4154403592990962 110-111 0.43758948887415394 112-113 0.43797916391945757 114-115 0.43113647012392603 116-117 0.4249484304045045 118-119 0.4671736183136049 120-121 0.4598165534582725 122-123 0.425322518447996 124-125 0.4351579165914594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3207801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.55694961165868 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49380102351364 54.905357579961944 2 10.351311876282141 13.779034859285492 3 3.229616586791723 6.448602852962212 4 1.4307095437725217 3.8089465205514763 5 0.7186470988568378 2.3915479373589625 6 0.4296333181451896 1.7157049864367464 7 0.2729866576371768 1.2718411451908884 8 0.20042825192017943 1.0671914451043372 9 0.14511185645052624 0.869238226604873 >10 0.6544170306077546 7.931019730175391 >50 0.05076842067638718 2.298177155319159 >100 0.02244892735579957 2.4683297821541674 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.194079901455511E-4 1.0450077788942813 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 13149 0.4099069736557847 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.117400362428966E-5 0.0 0.0 0.0 0.0 7 3.117400362428966E-5 0.0 0.0 0.0 0.0 8 3.117400362428966E-5 0.0 0.0 0.0 0.0 9 3.117400362428966E-5 0.0 0.0 0.0 0.0 10-11 3.117400362428966E-5 0.0 0.0 0.0 0.0 12-13 3.117400362428966E-5 0.0 0.0 0.0 0.0 14-15 3.117400362428966E-5 0.0 0.0 0.0 0.0 16-17 3.117400362428966E-5 0.0 0.0 3.117400362428966E-5 0.0 18-19 3.117400362428966E-5 0.0 0.0 9.352201087286899E-5 0.0 20-21 3.117400362428966E-5 0.0 0.0 1.4028301630930346E-4 0.0 22-23 3.117400362428966E-5 0.0 0.0 2.1821802537002764E-4 0.0 24-25 3.117400362428966E-5 0.0 0.0 3.117400362428966E-4 0.0 26-27 3.117400362428966E-5 0.0 0.0 6.702410779222277E-4 0.0 28-29 3.117400362428966E-5 0.0 0.0 0.0015898741848387728 0.0 30-31 3.117400362428966E-5 0.0 0.0 0.005564559646935705 0.0 32-33 3.117400362428966E-5 0.0 0.0 0.014885586730598314 0.0 34-35 3.117400362428966E-5 0.0 0.0 0.029054171377837966 0.0 36-37 3.117400362428966E-5 0.0 0.0 0.05474155036425264 0.0 38-39 3.117400362428966E-5 0.0 0.0 0.09152687464091444 0.0 40-41 3.117400362428966E-5 0.0 0.0 0.12873304796650414 0.0 42-43 3.117400362428966E-5 0.0 0.0 0.16939953569439004 0.0 44-45 3.117400362428966E-5 0.0 0.0 0.22096133768896511 0.0 46-47 3.117400362428966E-5 0.0 0.0 0.2872372693942049 0.0 48-49 3.117400362428966E-5 0.0 0.0 0.35868808570107685 0.0 50-51 3.117400362428966E-5 0.0 0.0 0.4351890905950837 0.0 52-53 3.117400362428966E-5 0.0 0.0 0.5102872653259974 0.0 54-55 3.117400362428966E-5 0.0 0.0 0.5974809534631357 0.0 56-57 3.117400362428966E-5 0.0 0.0 0.7025217586751796 0.0 58-59 3.117400362428966E-5 0.0 0.0 0.8245056348570251 0.0 60-61 3.117400362428966E-5 0.0 0.0 0.9593020265284535 0.0 62-63 3.117400362428966E-5 0.0 0.0 1.0941140052016942 0.0 64-65 3.117400362428966E-5 0.0 0.0 1.2384964029875918 0.0 66-67 3.117400362428966E-5 0.0 0.0 1.4076465466529875 0.0 68-69 3.117400362428966E-5 0.0 0.0 1.6014865011888206 0.0 70-71 6.234800724857932E-5 0.0 0.0 1.8150440130170171 0.0 72-73 6.234800724857932E-5 0.0 0.0 2.0346025205428893 0.0 74-75 6.234800724857932E-5 0.0 0.0 2.258120126529046 0.0 76-77 6.234800724857932E-5 0.0 0.0 2.5181892517646824 0.0 78-79 6.234800724857932E-5 0.0 0.0 2.810320839727901 0.0 80-81 6.234800724857932E-5 0.0 0.0 3.1317715780997637 0.0 82-83 6.234800724857932E-5 0.0 0.0 3.4646008277944924 0.0 84-85 6.234800724857932E-5 0.0 0.0 3.8087306538030257 0.0 86-87 6.234800724857932E-5 0.0 0.0 4.168946265681693 0.0 88-89 6.234800724857932E-5 0.0 0.0 4.573366614699602 0.0 90-91 6.234800724857932E-5 0.0 0.0 5.015943944153642 0.0 92-93 9.352201087286899E-5 0.0 0.0 5.48073275118999 0.0 94-95 9.352201087286899E-5 0.0 0.0 5.952270730010995 0.0 96-97 9.352201087286899E-5 0.0 0.0 6.427284610236109 0.0 98-99 1.0910901268501382E-4 0.0 0.0 6.936870460480559 0.0 100-101 1.4028301630930346E-4 0.0 0.0 7.5060921796582765 0.0 102-103 1.558700181214483E-4 0.0 0.0 8.111226351011176 0.0 104-105 1.558700181214483E-4 0.0 0.0 8.71081154971895 0.0 106-107 1.558700181214483E-4 0.0 0.0 9.304832188779791 0.0 108-109 1.558700181214483E-4 0.0 0.0 9.928546066292766 0.0 110-111 1.558700181214483E-4 0.0 0.0 10.59920799326392 0.0 112-113 1.558700181214483E-4 0.0 0.0 11.31197041212968 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3225 0.0 46.502205 1 GTATCAA 4490 0.0 42.811348 1 ATCAACG 4935 0.0 36.90611 3 TCAACGC 5015 0.0 36.20607 4 CAACGCA 5030 0.0 36.098667 5 AACGCAG 5310 0.0 34.307278 6 TACATGG 9700 0.0 32.947742 2 TATCAAC 5675 0.0 32.929634 2 GTACATG 9930 0.0 32.842335 1 ACATGGG 9950 0.0 30.926546 3 CATGGGG 6365 0.0 26.562714 4 CGCAGAG 6770 0.0 26.470638 8 GTACCGT 345 0.0 25.884005 6 ACGCAGA 7155 0.0 25.212711 7 GCAGAGT 7985 0.0 21.473562 9 GAGTACA 7540 0.0 20.99486 1 AGTACAT 7365 0.0 20.201815 2 AGAGTAC 6930 0.0 19.717243 10-11 ATGGGCG 430 4.9112714E-11 19.382906 5 CATGGGA 4310 0.0 19.33763 4 >>END_MODULE