FastQCFastQC Report
Thu 26 May 2016
SRR1364090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207801
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53310.16618861332108817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.16294651694416207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.069.1306461
GTATCAA79950.047.40631
ATCAACG97100.038.2936253
CAACGCA99000.037.5586975
TCAACGC99650.037.313714
AACGCAG102800.036.343966
TATCAAC104550.035.9063342
GTACATG100550.031.7672861
TACATGG98900.031.6413632
ACATGGG98900.030.0773413
CGCAGAG125500.029.4889818
ACGCAGA126500.029.3499367
GAGTACT88900.026.06907312-13
GCAGAGT143550.025.490899
CATGGGG64300.025.1665484
CAGAGTA134550.024.85244410-11
TGGTATC25650.024.1219082
CGCGGAA6850.023.88404382-83
AGAGTAC129300.023.56068610-11
GTACTTT97650.023.42591514-15
Produced by FastQC (version 0.11.5)