FastQCFastQC Report
Thu 26 May 2016
SRR1364089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629377
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67600.4148824980345248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.066.6073761
GTATCAA46500.048.5037421
GTACATG116550.040.1327861
TCAACGC55200.039.896254
ATCAACG55100.039.8557173
CAACGCA57600.038.338425
TACATGG122550.037.2441182
TATCAAC61000.036.4852562
AACGCAG59250.036.4670876
ACATGGG124350.034.9375843
CATGGGA92950.031.5694434
GTGGTAT12750.031.2718561
ACGCAGA71450.029.7414537
CGCAGAG72000.029.1008978
GAGTACA81450.026.887211
ATGGGAT36550.026.8707245
ATGGGAG36550.026.2193135
TGGTATC15000.026.1835352
CATGGGG35800.025.7702144
GCAGAGT78800.025.6076479
Produced by FastQC (version 0.11.5)