##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1629377 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.822605204320425 31.0 31.0 34.0 27.0 34.0 2 30.800650800888928 31.0 31.0 34.0 27.0 34.0 3 30.91413343873149 33.0 31.0 34.0 27.0 34.0 4 34.318251086151335 37.0 35.0 37.0 30.0 37.0 5 34.41813036516411 37.0 35.0 37.0 31.0 37.0 6 34.42773035338046 37.0 35.0 37.0 30.0 37.0 7 34.3222378860141 37.0 35.0 37.0 30.0 37.0 8 34.26164233323534 37.0 35.0 37.0 30.0 37.0 9 35.65204553642282 39.0 37.0 39.0 30.0 39.0 10-11 35.555225095235784 39.0 36.0 39.0 29.5 39.0 12-13 35.420432165177246 39.0 35.0 39.0 28.5 39.0 14-15 36.63740282328767 40.0 37.0 41.0 28.5 41.0 16-17 36.668790279965904 40.0 37.0 41.0 29.0 41.0 18-19 36.684196168228716 40.0 37.0 41.0 29.0 41.0 20-21 36.72870397704153 40.0 37.0 41.0 29.0 41.0 22-23 36.717349637315365 40.0 37.0 41.0 29.5 41.0 24-25 36.7491182212588 40.0 37.0 41.0 30.0 41.0 26-27 36.755568846252274 40.0 37.0 41.0 30.0 41.0 28-29 36.74273326553646 40.0 37.0 41.0 30.0 41.0 30-31 36.70876629533865 40.0 37.0 41.0 30.0 41.0 32-33 36.62683160496313 40.0 36.0 41.0 30.0 41.0 34-35 36.608347546332126 40.0 36.0 41.0 30.0 41.0 36-37 36.54612038834475 40.0 36.0 41.0 30.0 41.0 38-39 36.45326557328353 40.0 36.0 41.0 30.0 41.0 40-41 36.35930021106226 40.0 36.0 41.0 30.0 41.0 42-43 36.23198621313545 40.0 35.5 41.0 29.0 41.0 44-45 36.12060591256658 40.0 35.0 41.0 29.0 41.0 46-47 35.981301749073424 40.0 35.0 41.0 28.0 41.0 48-49 35.853648357623804 40.0 35.0 41.0 28.0 41.0 50-51 35.714395440711385 39.0 35.0 41.0 28.0 41.0 52-53 35.55303315316222 39.0 35.0 41.0 27.5 41.0 54-55 35.36115797633083 39.0 35.0 41.0 27.0 41.0 56-57 35.16746676797328 39.0 34.5 41.0 27.0 41.0 58-59 34.94735595261257 38.0 34.0 40.5 26.5 41.0 60-61 34.68870862912635 38.0 34.0 40.0 26.0 41.0 62-63 34.41589546188513 37.0 34.0 40.0 26.0 41.0 64-65 34.120485928057164 37.0 34.0 40.0 26.0 41.0 66-67 33.804623791792814 36.0 33.0 39.5 26.0 41.0 68-69 33.47450006965853 36.0 33.0 39.0 25.0 41.0 70-71 33.127722129378284 35.0 33.0 39.0 24.5 41.0 72-73 32.77751342997968 35.0 33.0 38.0 24.0 40.0 74-75 32.5093535750167 35.0 33.0 37.0 24.0 39.5 76-77 32.28570490438984 35.0 33.0 37.0 24.0 39.0 78-79 31.989535877823243 35.0 32.0 36.5 23.0 39.0 80-81 31.794455488201933 35.0 32.5 36.0 23.5 38.0 82-83 31.702779344497927 35.0 33.0 36.0 24.0 37.0 84-85 31.500857996645344 35.0 33.0 35.0 23.0 37.0 86-87 31.308798086630656 35.0 33.0 35.0 22.5 36.5 88-89 31.12671468911124 35.0 32.5 35.0 20.5 36.0 90-91 30.980561895742973 35.0 32.0 35.0 20.0 36.0 92-93 30.858138417321467 35.0 32.0 35.0 19.5 36.0 94-95 30.750524280138972 35.0 32.0 35.0 19.0 35.5 96-97 30.657640926562728 35.0 32.0 35.0 18.0 35.0 98-99 30.5722659642305 35.0 32.0 35.0 17.5 35.0 100-101 30.482829326791773 35.0 32.0 35.0 13.5 35.0 102-103 29.3890278308826 33.5 30.0 34.5 9.0 35.0 104-105 30.187995779982167 35.0 31.5 35.0 7.0 35.0 106-107 30.26731873593404 35.0 32.0 35.0 6.0 35.0 108-109 30.218525240015047 35.0 32.0 35.0 2.0 35.0 110-111 30.139661048363884 35.0 32.0 35.0 2.0 35.0 112-113 30.043057561264213 35.0 32.0 35.0 2.0 35.0 114-115 29.916211226744945 35.0 32.0 35.0 2.0 35.0 116-117 29.800990501277482 35.0 32.0 35.0 2.0 35.0 118-119 29.635052538485567 35.0 31.0 35.0 2.0 35.0 120-121 29.475756378051244 35.0 31.0 35.0 2.0 35.0 122-123 29.281121250637515 35.0 31.0 35.0 2.0 35.0 124-125 28.431472581238104 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7040933571806463 1101 2 0.6806489163576721 1101 3 0.7489767929146858 1101 4 0.8308165308914184 1101 5 0.7689161308335457 1101 6 0.8414456506546557 1101 7 0.84506416021852 1101 8 0.7745317864908827 1101 9 0.9463980319604062 1101 10-11 0.9577567703681353 1101 12-13 0.9816152821766693 1101 14-15 1.0465332994952448 1101 16-17 1.0493465680006224 1101 18-19 1.0442835705702649 1101 20-21 1.0440377432081576 1101 22-23 1.0974772809363813 1101 24-25 1.0595585776589331 1101 26-27 0.9680835515536543 1101 28-29 1.0570922516155434 1101 30-31 1.0561531648882223 1101 32-33 1.0586054049680413 1101 34-35 1.106230288429984 1101 36-37 1.0564694709744344 1101 38-39 1.0933635947360472 1101 40-41 1.1048466455703192 1101 42-43 1.0776206447813195 1101 44-45 1.0832105712575242 1101 46-47 1.127062953583824 1101 48-49 1.0246342070981598 1101 50-51 1.0321667139320354 1101 52-53 1.0260862678606415 1101 54-55 1.0443436367164125 1101 56-57 1.0614816446340356 1101 58-59 1.1800891393271797 1101 60-61 1.0883647364127498 1101 62-63 1.159317470941339 1101 64-65 1.1144998752179163 1101 66-67 1.1635176196299852 1101 68-69 1.2123004345540593 1101 70-71 1.263777088418415 1101 72-73 1.2795709777219386 1101 74-75 1.1984660781868044 1101 76-77 1.1213610348617635 1101 78-79 1.1622772580166227 1101 80-81 1.161084332106416 1101 82-83 1.1873711968591465 1101 84-85 1.103834883573903 1101 86-87 1.0396615706771577 1101 88-89 1.0947503853745921 1101 90-91 1.1627744669330617 1101 92-93 1.1876576782121262 1101 94-95 1.2635533744293177 1101 96-97 1.2003931288377956 1101 98-99 1.1738007117504559 1101 100-101 1.1816905903855428 1101 102-103 1.333011649495944 1101 104-105 1.2383669733657712 1101 106-107 1.2264158500242068 1101 108-109 1.2160214131071712 1101 110-111 1.2877573762063967 1101 112-113 1.2887931196342741 1101 114-115 1.2831315928511238 1101 116-117 1.2936288794750723 1101 118-119 1.2707228183372656 1101 120-121 1.2269330713528248 1101 122-123 1.2423575063267798 1101 124-125 1.1991144624938954 1102 1 0.39668880424013864 1102 2 0.37239092305859245 1102 3 0.3615182017076215 1102 4 0.3733380723479627 1102 5 0.528682201924326 1102 6 0.42361542883419645 1102 7 0.4887723372913868 1102 8 0.4154090439618656 1102 9 0.6877637734466333 1102 10-11 0.5198316465720794 1102 12-13 0.507135388461549 1102 14-15 0.6431245724521659 1102 16-17 0.5850294813139598 1102 18-19 0.5554248448413048 1102 20-21 0.6327269654280414 1102 22-23 0.6461788152041166 1102 24-25 0.6143891130183121 1102 26-27 0.5937147476028386 1102 28-29 0.6029470412649829 1102 30-31 0.613581871801145 1102 32-33 0.6507926036217881 1102 34-35 0.6065554878899775 1102 36-37 0.5773685799242116 1102 38-39 0.6649523597517657 1102 40-41 0.7034673149296466 1102 42-43 0.7579879813551713 1102 44-45 0.7620222439377002 1102 46-47 0.834715024587986 1102 48-49 0.8012478127624405 1102 50-51 0.7473708344331484 1102 52-53 0.8167625753864272 1102 54-55 0.7646877410556456 1102 56-57 0.7466642283058036 1102 58-59 0.7071261335883392 1102 60-61 0.8296068391742963 1102 62-63 0.7680512512249607 1102 64-65 0.6366208420135138 1102 66-67 0.686501928987397 1102 68-69 0.7444198503129584 1102 70-71 0.7391067207338793 1102 72-73 0.6183002714745882 1102 74-75 0.5793560845131154 1102 76-77 0.5834811280424148 1102 78-79 0.6177773118772052 1102 80-81 0.5582766965637624 1102 82-83 0.5971111335368704 1102 84-85 0.6052999469350482 1102 86-87 0.5829407694982187 1102 88-89 0.601659624555932 1102 90-91 0.664907697653657 1102 92-93 0.6157736979780388 1102 94-95 0.6517161325945615 1102 96-97 0.712521939659073 1102 98-99 0.674856453195968 1102 100-101 0.7044579767110264 1102 102-103 0.6369728269363897 1102 104-105 0.6272092817970041 1102 106-107 0.5900365064273778 1102 108-109 0.6396196220484747 1102 110-111 0.6407520430833209 1102 112-113 0.7071744407103466 1102 114-115 0.6187467824977482 1102 116-117 0.7291973714417033 1102 118-119 0.6793157652292514 1102 120-121 0.6878303812481086 1102 122-123 0.6708958041621038 1102 124-125 0.894025729498388 1103 1 0.29686582326153754 1103 2 0.30204812518065083 1103 3 0.33367191503454663 1103 4 0.24705400568889502 1103 5 0.3645057476261897 1103 6 0.19397668881866537 1103 7 0.2983525415691659 1103 8 0.41655912459783195 1103 9 0.30803027938437566 1103 10-11 0.4534427260080065 1103 12-13 0.4459785745867322 1103 14-15 0.3638330829402676 1103 16-17 0.36402123309637346 1103 18-19 0.30350587176882726 1103 20-21 0.3295843347950651 1103 22-23 0.3901617974859235 1103 24-25 0.402923873363882 1103 26-27 0.44582512927974705 1103 28-29 0.3811965077644004 1103 30-31 0.302974305595491 1103 32-33 0.11930358043012745 1103 34-35 0.4025041415137025 1103 36-37 0.3385859386483716 1103 38-39 0.31260278519471996 1103 40-41 0.3288128006730986 1103 42-43 0.3333049594341375 1103 44-45 0.36108637803775423 1103 46-47 0.3293232749775896 1103 48-49 0.3168442202537136 1103 50-51 0.34563811143534195 1103 52-53 0.28033683920993013 1103 54-55 0.44431616401164575 1103 56-57 0.4370289092100137 1103 58-59 0.4388339870426563 1103 60-61 0.4102835296788001 1103 62-63 0.36847316465939883 1103 64-65 0.3631058198318371 1103 66-67 0.3831779353079554 1103 68-69 0.3011446726151874 1103 70-71 0.3933619647866351 1103 72-73 0.5013333240752971 1103 74-75 0.5501772681513444 1103 76-77 0.45319784330786206 1103 78-79 0.4366895680009222 1103 80-81 0.3671455667371468 1103 82-83 0.3579532770960441 1103 84-85 0.2851781513322891 1103 86-87 0.31264548439442663 1103 88-89 0.402920749332619 1103 90-91 0.3142506638625733 1103 92-93 0.32540250103623336 1103 94-95 0.3446253261806085 1103 96-97 0.37307982107804705 1103 98-99 0.3957756488997255 1103 100-101 0.4045804239175439 1103 102-103 0.42027113656763504 1103 104-105 0.3811571860764147 1103 106-107 0.33131696211561135 1103 108-109 0.3068138162589733 1103 110-111 0.2135562741637358 1103 112-113 0.1852151225050811 1103 114-115 0.22496606888303106 1103 116-117 0.2139900256953169 1103 118-119 0.260058430614432 1103 120-121 0.3852383557921186 1103 122-123 0.3108830978887873 1103 124-125 0.31607111370025365 1104 1 0.31512393734747235 1104 2 0.4015239318155821 1104 3 0.3448804694338179 1104 4 0.30045626158436534 1104 5 0.2749582451597945 1104 6 0.28337234342388484 1104 7 0.3389186522180694 1104 8 0.4037129773963315 1104 9 0.43208907269397656 1104 10-11 0.34554209088386756 1104 12-13 0.39789281236957663 1104 14-15 0.3653251927617305 1104 16-17 0.3651255398472344 1104 18-19 0.4576832791711851 1104 20-21 0.35206600166631574 1104 22-23 0.30621311660738826 1104 24-25 0.4539088386070844 1104 26-27 0.46887918075344004 1104 28-29 0.4637344583638381 1104 30-31 0.5596817529602021 1104 32-33 -0.2805791239166311 1104 34-35 0.3718636072274535 1104 36-37 0.5133982883348978 1104 38-39 0.5264377620311009 1104 40-41 0.5899733911521494 1104 42-43 0.573686943185507 1104 44-45 0.5324835147508367 1104 46-47 0.47835876827856083 1104 48-49 0.5165214298901475 1104 50-51 0.47889051986062015 1104 52-53 0.3861943477229559 1104 54-55 0.5117125187537113 1104 56-57 0.47583876750049114 1104 58-59 0.3980187367891972 1104 60-61 0.46391901297781146 1104 62-63 0.5553969724945631 1104 64-65 0.5711869447789866 1104 66-67 0.4965237256215147 1104 68-69 0.48165722734179184 1104 70-71 0.5312084529638739 1104 72-73 0.4368598133436592 1104 74-75 0.4917699430832627 1104 76-77 0.49404446332904683 1104 78-79 0.5153303932531301 1104 80-81 0.5528877799879162 1104 82-83 0.47181416808837184 1104 84-85 0.4259402093709035 1104 86-87 0.4120390022183642 1104 88-89 0.4091937936094574 1104 90-91 0.36499194511300104 1104 92-93 0.3696907028211278 1104 94-95 0.33635507335846526 1104 96-97 0.4593098569486962 1104 98-99 0.3790719295363978 1104 100-101 0.4488704442396987 1104 102-103 0.4371944443050566 1104 104-105 0.5872606517537946 1104 106-107 0.48430868322473586 1104 108-109 0.5180319179788562 1104 110-111 0.5470573675592618 1104 112-113 0.5709077806155314 1104 114-115 0.6519558896207194 1104 116-117 0.6351905330672132 1104 118-119 0.6086929364496605 1104 120-121 0.5983536465080341 1104 122-123 0.6081134656424858 1104 124-125 0.6111046884444065 1105 1 0.25365806159333815 1105 2 0.2526323786288103 1105 3 0.3264994317886831 1105 4 0.2030904261437385 1105 5 0.1963160248805309 1105 6 0.27097044397728354 1105 7 0.18630150309824245 1105 8 0.2012693857599004 1105 9 0.23451660873617186 1105 10-11 0.31907741057344197 1105 12-13 0.3069350787214091 1105 14-15 0.3616341512597643 1105 16-17 0.3186508771456076 1105 18-19 0.42411670342804797 1105 20-21 0.3353305751721365 1105 22-23 0.3576535826384557 1105 24-25 0.4104332561991271 1105 26-27 0.3904300638983855 1105 28-29 0.34982150062669604 1105 30-31 0.32109213220380894 1105 32-33 -0.00278514511350636 1105 34-35 0.4209438136465522 1105 36-37 0.4357640801461997 1105 38-39 0.42355098363843524 1105 40-41 0.4535746609402338 1105 42-43 0.33580289598904045 1105 44-45 0.31705583385926417 1105 46-47 0.3584025372245989 1105 48-49 0.3838698485013694 1105 50-51 0.35211135932387805 1105 52-53 0.4834735353098836 1105 54-55 0.4551112262783761 1105 56-57 0.4005786057927381 1105 58-59 0.3878775348185428 1105 60-61 0.4031289555790565 1105 62-63 0.36418950454903154 1105 64-65 0.30042095331986474 1105 66-67 0.3089811215786469 1105 68-69 0.33958834186180553 1105 70-71 0.44234763227868257 1105 72-73 0.349968595230159 1105 74-75 0.3584541487414654 1105 76-77 0.3830713372524954 1105 78-79 0.34066254335897383 1105 80-81 0.28734526669405724 1105 82-83 0.244176731329091 1105 84-85 0.3089910864077403 1105 86-87 0.21495187095166557 1105 88-89 0.20705464745293867 1105 90-91 0.2115385962127192 1105 92-93 0.26068403800434226 1105 94-95 0.24324580282344854 1105 96-97 0.16315997099576762 1105 98-99 0.14721850315042317 1105 100-101 0.19690311039328634 1105 102-103 0.23477650671594574 1105 104-105 0.3095922381686691 1105 106-107 0.30950907274741013 1105 108-109 0.19500182595587034 1105 110-111 0.23608520132801658 1105 112-113 0.2717342074255775 1105 114-115 0.32606423695906983 1105 116-117 0.3211861282498383 1105 118-119 0.37319829325906895 1105 120-121 0.4564731524843779 1105 122-123 0.41581875473916696 1105 124-125 0.46859889883202754 1106 1 0.2102122615378761 1106 2 0.2677978879741083 1106 3 0.25499822015688167 1106 4 0.3345605382642276 1106 5 0.2484769521225445 1106 6 0.24258170212257113 1106 7 0.2965781570460848 1106 8 0.2028107837172186 1106 9 0.3962194716087737 1106 10-11 0.3958543715449423 1106 12-13 0.347371571665974 1106 14-15 0.4389628722796317 1106 16-17 0.3540334449224787 1106 18-19 0.349732541300547 1106 20-21 0.465803496839186 1106 22-23 0.36737812012572846 1106 24-25 0.44471281303957966 1106 26-27 0.41564529444447373 1106 28-29 0.39337057536361897 1106 30-31 0.4968737866149908 1106 32-33 0.5161674137055385 1106 34-35 0.5551301262635135 1106 36-37 0.49150983536161164 1106 38-39 0.49737965187536304 1106 40-41 0.5046091558718828 1106 42-43 0.4371694143542868 1106 44-45 0.44813830526906884 1106 46-47 0.3567722059343552 1106 48-49 0.3864958427641483 1106 50-51 0.39043941945367067 1106 52-53 0.5141320289353715 1106 54-55 0.4710541385463216 1106 56-57 0.5314985702058266 1106 58-59 0.4850640168161675 1106 60-61 0.5017873351526347 1106 62-63 0.42579331877070103 1106 64-65 0.4485495176541363 1106 66-67 0.5413522098273305 1106 68-69 0.5654334376467389 1106 70-71 0.5828019628058669 1106 72-73 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2216 104-105 -2.6082239273392176 2216 106-107 -2.5592224257093044 2216 108-109 -2.659272775036861 2216 110-111 -2.660306848832736 2216 112-113 -2.7426012163085858 2216 114-115 -2.7933966579186453 2216 116-117 -2.856070637573744 2216 118-119 -2.909199595397311 2216 120-121 -2.9042315225782396 2216 122-123 -2.9998547914682234 2216 124-125 -3.1664032924044676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35708.0 3 6802.0 4 3805.0 5 3226.0 6 3605.0 7 3891.0 8 4433.0 9 4869.0 10 5532.0 11 5996.0 12 6736.0 13 6991.0 14 7259.0 15 6310.0 16 5731.0 17 5162.0 18 5037.0 19 4921.0 20 5039.0 21 5648.0 22 6532.0 23 8629.0 24 11223.0 25 13939.0 26 17042.0 27 19530.0 28 23145.0 29 28956.0 30 33941.0 31 39370.0 32 50900.0 33 66999.0 34 92235.0 35 138395.0 36 256885.0 37 471417.0 38 212418.0 39 1120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72457489940956 18.341714585228235 12.547705304700388 23.386005210661814 2 15.98946351353101 20.161882558378288 39.57243618636904 24.276217741721663 3 19.090887791114934 25.69506273013587 28.824663906063876 26.389385572685324 4 12.461141972834833 15.919121047147533 36.519270252107056 35.10046672791057 5 13.48094014870026 39.37763675355441 33.234347811610164 13.907075286135163 6 32.451655374994374 35.58218694221596 17.69311782805028 14.273039854739386 7 29.50911808817206 33.18479436411522 20.08996242208799 17.216125125624732 8 27.294602680612485 35.08495146379853 19.383383855242236 18.237062000346754 9 27.915985364077823 15.976355457927893 19.055637483263094 37.05202169473119 10-11 25.471048067099105 27.18212953439983 26.684891129969184 20.661931268531884 12-13 25.77780068268275 24.790468981015295 27.139000095974296 22.292730240327657 14-15 22.844082043699952 26.182360197241554 24.77527705083024 26.19828070822825 16-17 22.916021994790384 29.7640801736827 25.106091266101117 22.213806565425802 18-19 22.853004782808178 28.26105536499266 26.882893245660345 22.003046606538817 20-21 23.97742882638043 27.33619916147991 26.549824310664654 22.136547701475003 22-23 23.498459091637066 27.623841232899498 26.37124180828304 22.506457867180394 24-25 23.061278835006938 27.71027234249226 26.65260433428814 22.575844488212667 26-27 22.8539873597599 28.014199826390335 26.444954843280712 22.686857970569058 28-29 22.679446090584747 27.985759823673074 26.601757592436503 22.733036493305672 30-31 22.792927049008355 27.71539116590766 26.716495735989238 22.775186049094746 32-33 22.58570832633959 27.856471105143115 26.650729159467225 22.907091409050068 34-35 23.034081232181865 27.923192269243586 26.415145568761027 22.62758092981352 36-37 23.183778771374904 27.668349490850858 26.253506654664555 22.89436508310968 38-39 22.835982672324178 27.753259420659788 26.496688480400316 22.914069426615715 40-41 22.975740359108656 27.62741359458547 26.726143042327593 22.67070300397828 42-43 22.8941783884994 27.673566009516414 26.35484633795691 23.077409264027274 44-45 22.711835246156138 27.854272532773077 26.52216717176124 22.911725049309542 46-47 22.89420795800657 27.852807157432814 26.25868978969368 22.994295094866942 48-49 22.901534079660138 27.79152478501113 26.314557566689263 22.99238356863947 50-51 22.823075042264325 27.8224290910526 26.2697103764431 23.08478549023997 52-53 23.05908978088574 27.763332547093455 26.308172115617346 22.86940555640346 54-55 22.66091800761795 27.614897391985277 26.52544737341055 23.198737226986225 56-57 22.674518246168223 27.99806916245298 26.281275930987967 23.04613666039083 58-59 22.85767313606957 27.604193643065557 26.360196277195293 23.17793694366958 60-61 22.819957659293266 27.724654074206295 26.586011535638303 22.869376730862136 62-63 22.729477365152476 27.805694625894535 26.43413235782475 23.030695651128237 64-65 22.464469472560562 27.728041188987284 26.515071394059625 23.292417944392525 66-67 22.55879620307382 28.08850243559931 26.10101515939708 23.251686201929793 68-69 22.564951035192056 27.743270519191952 26.535734055791043 23.15604438982494 70-71 22.55149631979711 27.72724805689975 26.496659014739315 23.22459660856383 72-73 22.679105262459903 27.868779147176102 26.258388511405663 23.193727078958336 74-75 22.451061478218126 28.00022326783938 26.270575950846535 23.27813930309596 76-77 22.535521815124973 27.864780886617595 26.307266938777524 23.292430359479905 78-79 22.734195110728137 27.899092228521305 26.392883644014425 22.97382901673613 80-81 22.51845094694096 28.29107162902464 26.217429437386436 22.973047986647966 82-83 22.577568208522095 28.1433897792841 26.15512716529484 23.12391484689897 84-85 22.702583515153385 27.78745942778724 26.208670310777364 23.301286746282006 86-87 22.599804873716785 27.833062187560515 26.276893076470508 23.29023986225219 88-89 22.643320665534336 27.851796619535545 26.336471296395658 23.168411418534458 90-91 22.632910096662727 27.89763882221377 26.325277746174546 23.144173334948952 92-93 22.72457487195979 27.599923165080686 26.38203419523901 23.293467767720514 94-95 22.576065277692564 27.50284646478785 26.489187441825905 23.43190081569368 96-97 22.636878342077015 27.457515199456452 26.43897744234811 23.46662901611842 98-99 22.52538923587614 27.521204434040992 26.245425376786248 23.707980953296627 100-101 22.349868727582 27.628160090719717 26.431855440101565 23.590115741596716 102-103 22.576413024723312 27.390217900367276 26.49464492592866 23.538724148980748 104-105 22.31484691197111 27.576089518712127 26.404414925331743 23.704648643985017 106-107 22.397978190114095 27.63232615673402 26.3831153484815 23.58658030467038 108-109 22.488905269600178 27.6274118191968 26.16257766927611 23.72110524192691 110-111 22.31054734278178 28.034360386424883 26.231358661192694 23.42373360960064 112-113 22.38925401621219 28.084140870580534 26.242037391969607 23.28456772123767 114-115 22.209184593406288 28.29612369411812 26.037500905390893 23.4571908070847 116-117 22.248682540186415 28.297085916655856 26.12313468370332 23.331096859454416 118-119 22.18165616800328 28.433371258020458 25.82868524668005 23.556287327296214 120-121 22.25909606066548 28.63805429456894 25.85626849087342 23.246581153892162 122-123 22.225698025120707 28.60887478295066 25.898108087950213 23.26731910397842 124-125 22.377477315865363 28.721545042470915 25.711593551596657 23.189384090067065 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7806.0 1 4412.0 2 1226.5 3 1439.0 4 1334.5 5 1093.0 6 930.5 7 889.5 8 858.0 9 846.0 10 772.0 11 700.0 12 712.0 13 720.5 14 744.5 15 800.5 16 912.5 17 1040.0 18 1203.5 19 1515.5 20 1849.5 21 2275.0 22 2950.5 23 3770.0 24 4934.0 25 6436.5 26 8288.0 27 10386.5 28 12464.5 29 15003.0 30 18119.0 31 21511.5 32 25587.0 33 28458.5 34 30869.0 35 33205.0 36 36395.5 37 41048.5 38 43310.0 39 44051.5 40 44158.5 41 45597.0 42 47719.5 43 50224.5 44 52651.5 45 54357.5 46 55204.0 47 55869.5 48 58109.0 49 59484.0 50 59446.5 51 58191.5 52 57548.0 53 58403.0 54 58314.5 55 55370.5 56 51348.5 57 47269.0 58 43357.0 59 38365.5 60 32688.5 61 27930.5 62 23575.5 63 18797.5 64 14621.0 65 11534.0 66 8464.0 67 6716.5 68 4858.0 69 2819.0 70 2085.5 71 1723.5 72 1417.0 73 1225.0 74 964.5 75 760.5 76 502.0 77 234.0 78 137.0 79 102.0 80 64.5 81 40.0 82 28.5 83 19.0 84 15.0 85 12.0 86 13.5 87 14.0 88 11.5 89 9.0 90 7.0 91 5.0 92 4.5 93 5.0 94 4.5 95 3.5 96 3.0 97 2.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4726960058967323 2 0.4654539741263072 3 0.46085098783154543 4 0.45704585249454244 5 0.465883586180485 6 0.5257224080123876 7 0.5203215707598672 8 0.5295275433493906 9 0.5343760222465397 10-11 0.5540461170128215 12-13 0.5616563876868276 14-15 0.5416794271675616 16-17 0.5352045597795968 18-19 0.5453311296280725 20-21 0.546405159763517 22-23 0.5564396698860975 24-25 0.5506705937299963 26-27 0.556992028241469 28-29 0.5370764408727998 30-31 0.5423545318241266 32-33 0.5450549504503869 34-35 0.5423545318241266 36-37 0.4907090255968999 38-39 0.45802782290409155 40-41 0.4568924196180503 42-43 0.46965803494218955 44-45 0.47462926014053225 46-47 0.47809684314925277 48-49 0.49184442888294116 50-51 0.4748747527429195 52-53 0.4746906332911291 54-55 0.4633366004307167 56-57 0.44698065579666335 58-59 0.46576083987929123 60-61 0.4623546300211676 62-63 0.47843439547753525 64-65 0.47739105191738934 66-67 0.4853081883443795 68-69 0.4946369072350966 70-71 0.49092383162398884 72-73 0.5024312973608932 74-75 0.4913534436781665 76-77 0.4896963686120523 78-79 0.5004366699664964 80-81 0.4941152354550236 82-83 0.49479034011158873 84-85 0.49340944422316013 86-87 0.48257708314282083 88-89 0.48748693519056674 90-91 0.47303355822501486 92-93 0.47370866288157987 94-95 0.46818507932786585 96-97 0.4568617330427519 98-99 0.4197309769316738 100-101 0.4171533046066074 102-103 0.4061061374991791 104-105 0.41116942242341703 106-107 0.4095123473573028 108-109 0.42178697747666744 110-111 0.446581730317784 112-113 0.4458145659353237 114-115 0.43878734019198745 116-117 0.43154530842156236 118-119 0.47515093192060526 120-121 0.46833851220435785 122-123 0.4326807117076036 124-125 0.4423776695019016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1629377.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.82939084527148 #Duplication Level Percentage of deduplicated Percentage of total 1 83.92847179043305 60.28531003281358 2 10.063520342723326 14.457130719536282 3 2.873288282485773 6.191596411614282 4 1.200218567184314 3.4484387424813523 5 0.6025114389847828 2.1639014819792446 6 0.3356435669050572 1.4465443771154627 7 0.22094528169650726 1.1109255496077917 8 0.14944613713820173 0.8587699995852747 9 0.10154141427003886 0.6564292139325824 >10 0.48942478065861755 6.326080181828201 >50 0.026162523567219143 1.2751828323177608 >100 0.008504933128289746 0.942290815335414 >500 1.6047041242528022E-4 0.05820878446464668 >1k 0.0 0.0 >5k 1.6047041242528022E-4 0.7791908573881416 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6760 0.4148824980345248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.205972589523481E-5 0.0 12-13 0.0 0.0 0.0 1.227463011936464E-4 0.0 14-15 0.0 0.0 0.0 1.8411945179046962E-4 0.0 16-17 0.0 0.0 0.0 2.1480602708888122E-4 0.0 18-19 0.0 0.0 0.0 2.761791776857044E-4 0.0 20-21 0.0 0.0 0.0 3.06865752984116E-4 0.0 22-23 0.0 0.0 0.0 5.523583553714088E-4 0.0 24-25 0.0 0.0 0.0 0.0011047167107428176 0.0 26-27 0.0 0.0 0.0 0.001994627394396754 0.0 28-29 0.0 0.0 0.0 0.004204060815882389 0.0 30-31 0.0 0.0 0.0 0.01205982409227576 0.0 32-33 0.0 0.0 0.0 0.02832370900043391 0.0 34-35 0.0 0.0 0.0 0.05228992430849337 0.0 36-37 0.0 0.0 0.0 0.0936247412354538 0.0 38-39 0.0 0.0 0.0 0.1571152655278674 0.0 40-41 0.0 0.0 0.0 0.22658967200347124 0.0 42-43 0.0 0.0 0.0 0.2970767354639227 0.0 44-45 0.0 0.0 0.0 0.38051353370030383 0.0 46-47 0.0 0.0 0.0 0.47932430616118926 0.0 48-49 0.0 0.0 0.0 0.5893970517565916 0.0 50-51 0.0 0.0 0.0 0.7195695041724537 0.0 52-53 0.0 0.0 0.0 0.8363932963335066 0.0 54-55 0.0 0.0 0.0 0.965399658888029 0.0 56-57 0.0 0.0 0.0 1.120029311816725 0.0 58-59 0.0 0.0 0.0 1.302522375116379 0.0 60-61 0.0 0.0 0.0 1.5094726389288666 0.0 62-63 0.0 0.0 0.0 1.7110527520641325 0.0 64-65 0.0 0.0 0.0 1.9128783578017856 0.0 66-67 0.0 0.0 0.0 2.1446847476059867 0.0 68-69 0.0 0.0 0.0 2.4150641625602915 0.0 70-71 0.0 0.0 0.0 2.729939111697293 0.0 72-73 0.0 0.0 0.0 3.0442003293283264 0.0 74-75 0.0 0.0 0.0 3.3568965316191406 0.0 76-77 0.0 0.0 0.0 3.6972720248291218 0.0 78-79 0.0 0.0 0.0 4.088617919609765 0.0 80-81 0.0 0.0 0.0 4.5292157677443585 0.0 82-83 0.0 0.0 0.0 4.989667830097025 0.0 84-85 0.0 0.0 0.0 5.443737084787621 0.0 86-87 0.0 0.0 0.0 5.902317266047085 0.0 88-89 0.0 0.0 0.0 6.411837162301911 0.0 90-91 0.0 0.0 0.0 6.988253792707274 0.0 92-93 0.0 0.0 0.0 7.606588284970268 0.0 94-95 0.0 0.0 0.0 8.217373879709852 0.0 96-97 0.0 0.0 0.0 8.810484007077552 0.0 98-99 0.0 0.0 0.0 9.443302562881396 0.0 100-101 0.0 0.0 0.0 10.16612484403548 0.0 102-103 0.0 0.0 0.0 10.93350403252286 0.0 104-105 0.0 0.0 0.0 11.686613963496477 0.0 106-107 0.0 0.0 0.0 12.426436607365883 0.0 108-109 0.0 0.0 0.0 13.177152985466224 0.0 110-111 0.0 0.0 0.0 14.010446937694592 0.0 112-113 0.0 0.0 0.0 14.890016245472964 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2725 0.0 66.607376 1 GTATCAA 4650 0.0 48.503742 1 GTACATG 11655 0.0 40.132786 1 TCAACGC 5520 0.0 39.89625 4 ATCAACG 5510 0.0 39.855717 3 CAACGCA 5760 0.0 38.33842 5 TACATGG 12255 0.0 37.244118 2 TATCAAC 6100 0.0 36.485256 2 AACGCAG 5925 0.0 36.467087 6 ACATGGG 12435 0.0 34.937584 3 CATGGGA 9295 0.0 31.569443 4 GTGGTAT 1275 0.0 31.271856 1 ACGCAGA 7145 0.0 29.741453 7 CGCAGAG 7200 0.0 29.100897 8 GAGTACA 8145 0.0 26.88721 1 ATGGGAT 3655 0.0 26.870724 5 ATGGGAG 3655 0.0 26.219313 5 TGGTATC 1500 0.0 26.183535 2 CATGGGG 3580 0.0 25.770214 4 GCAGAGT 7880 0.0 25.607647 9 >>END_MODULE