FastQCFastQC Report
Thu 26 May 2016
SRR1364089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629377
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103350.6342915114181679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99790.6124426698056987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60490.37124618796018355No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.17055598550857168No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.11605662777859267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51100.083.401651
GTATCAA93300.053.025641
GTGGTAT16150.047.9800071
TGGTATC17950.041.7646142
ATCAACG124450.039.5844423
TCAACGC125950.039.1142124
TATCAAC126850.039.0243152
CAACGCA128700.038.2784355
GTACATG113000.037.820751
AACGCAG133050.037.16116
TACATGG120300.035.2635732
ACATGGG116850.034.2669453
ACGCAGA152850.032.2315067
CGCAGAG154350.031.9182788
CATGGGA86500.030.6775844
GCAGAGT164800.029.533299
ATGGGAG34050.028.831635
GAGTACT109950.028.62614312-13
CAGAGTA163200.027.3614810-11
GTACTTT118200.026.50147214-15
Produced by FastQC (version 0.11.5)