FastQCFastQC Report
Thu 26 May 2016
SRR1364088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252491
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN134390.41319099730022313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.044.79591
GTATCAA48100.043.4236031
TCAACGC53050.038.2643134
ATCAACG53850.037.689443
CAACGCA55150.036.808995
AACGCAG57900.034.958456
TATCAAC60150.033.934052
GTACATG104550.030.7350221
TACATGG102700.030.5943132
ACATGGG102700.028.9188583
ACGCAGA70400.028.5844217
CGCAGAG70700.028.2104978
CATGGGG64800.024.803544
TAAGGTG19000.021.6192475
GAGTACA75700.021.4600411
GCAGAGT90700.020.939629
TGGTATC20950.020.7355732
TAGCCCT29150.019.4003684
AGAGTAC73500.019.23896810-11
CAGAGTA77500.019.05265410-11
Produced by FastQC (version 0.11.5)