FastQCFastQC Report
Thu 26 May 2016
SRR1364088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252491
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.15517337326990296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46130.1418297544866381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.071.297651
GTATCAA82050.051.855371
TCAACGC100750.041.513514
ATCAACG101200.041.2694933
CAACGCA103750.040.255785
TATCAAC105400.039.7949262
AACGCAG109850.038.3459136
CGCAGAG127850.032.4417848
ACGCAGA128650.032.378817
TACATGG94700.028.8992822
TGGTATC21450.028.2912462
GTACATG99550.028.014231
GCAGAGT152600.026.7511399
ACATGGG100800.026.0285873
TATACCG2308.29823E-925.8673155
GAGTACT99900.025.43512-13
CAGAGTA140250.025.33052310-11
GTGGTAT25650.025.0905691
GTACTTT104150.024.10640114-15
AGAGTAC134850.023.76333810-11
Produced by FastQC (version 0.11.5)