FastQCFastQC Report
Thu 26 May 2016
SRR1364087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780755
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114940.413340981136418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.060.8819771
GTATCAA59750.051.6981961
ATCAACG71700.041.9250073
TCAACGC71800.041.7897574
CAACGCA72550.041.357755
AACGCAG74200.040.1193056
GTACATG150750.038.928311
TATCAAC82000.037.015752
TACATGG157600.036.441932
ACATGGG158100.034.8265763
ACGCAGA91100.032.417697
CGCAGAG91750.031.7986558
CATGGGA85900.031.5342164
CATGGGG71250.028.1584554
AGTACAT105700.027.252142
GAGTACA112600.026.3759481
GCAGAGT104450.026.0511269
ATGGGAG39850.025.3971145
GGACCGA6900.023.2971086
CAGAGTA98850.022.40706610-11
Produced by FastQC (version 0.11.5)