##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2780755 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.175402363746535 31.0 31.0 34.0 28.0 34.0 2 31.1519763517462 33.0 31.0 34.0 28.0 34.0 3 31.25743440180814 33.0 31.0 34.0 28.0 34.0 4 34.723029536942306 37.0 35.0 37.0 32.0 37.0 5 34.756375876335746 37.0 35.0 37.0 32.0 37.0 6 34.80370510886432 37.0 35.0 37.0 32.0 37.0 7 34.6956545974025 37.0 35.0 37.0 32.0 37.0 8 34.62611988470757 37.0 35.0 37.0 31.0 37.0 9 36.14015689983476 39.0 37.0 39.0 31.0 39.0 10-11 36.064459831952114 39.0 37.0 39.0 31.0 39.0 12-13 35.972992226931176 39.0 37.0 39.0 30.0 39.0 14-15 37.240312253326884 40.0 38.0 41.0 31.0 41.0 16-17 37.26127670362905 40.0 38.0 41.0 31.0 41.0 18-19 37.273733572357145 40.0 38.0 41.0 31.0 41.0 20-21 37.302409237778946 40.0 38.0 41.0 31.0 41.0 22-23 37.26842224503777 40.0 37.5 41.0 31.0 41.0 24-25 37.293395139089924 40.0 37.0 41.0 31.0 41.0 26-27 37.27387040569917 40.0 37.0 41.0 31.0 41.0 28-29 37.248553180700924 40.0 37.0 41.0 31.0 41.0 30-31 37.20660881667029 40.0 37.0 41.0 31.0 41.0 32-33 37.11999187271083 40.0 37.0 41.0 30.0 41.0 34-35 37.09437562820169 40.0 37.0 41.0 30.0 41.0 36-37 37.03242806360143 40.0 37.0 41.0 30.0 41.0 38-39 36.94910824578217 40.0 37.0 41.0 30.0 41.0 40-41 36.8604114709854 40.0 37.0 41.0 30.0 41.0 42-43 36.746613239929445 40.0 36.5 41.0 30.0 41.0 44-45 36.64133985194668 40.0 36.0 41.0 30.0 41.0 46-47 36.517675775104244 40.0 36.0 41.0 30.0 41.0 48-49 36.39784015492195 40.0 36.0 41.0 30.0 41.0 50-51 36.256390260918344 40.0 35.0 41.0 29.5 41.0 52-53 36.10706552716798 39.5 35.0 41.0 29.0 41.0 54-55 35.921445254975715 39.0 35.0 41.0 29.0 41.0 56-57 35.72731128776178 39.0 35.0 41.0 28.0 41.0 58-59 35.507065347360694 39.0 35.0 41.0 28.0 41.0 60-61 35.2644393339219 38.0 34.5 40.5 28.0 41.0 62-63 34.99087100445742 38.0 34.0 40.0 28.0 41.0 64-65 34.69689544745941 37.0 34.0 40.0 27.5 41.0 66-67 34.3866784380501 37.0 34.0 40.0 27.0 41.0 68-69 34.05540635546821 36.0 34.0 39.0 26.0 41.0 70-71 33.71419848206692 36.0 33.5 39.0 26.0 41.0 72-73 33.36576001841226 35.0 33.0 38.5 26.0 40.0 74-75 33.089819851083604 35.0 33.0 37.0 26.0 40.0 76-77 32.86131859872589 35.0 33.0 37.0 26.0 39.0 78-79 32.57232963709497 35.0 33.0 36.5 26.0 39.0 80-81 32.37599860469549 35.0 33.0 36.0 26.0 38.5 82-83 32.28494653430453 35.0 33.0 36.0 26.0 37.0 84-85 32.08101990286811 35.0 33.0 35.5 26.0 37.0 86-87 31.8979066836165 35.0 33.0 35.0 25.5 36.5 88-89 31.728280808629314 35.0 33.0 35.0 25.0 36.0 90-91 31.589826863567627 35.0 33.0 35.0 25.0 36.0 92-93 31.470641246711775 35.0 33.0 35.0 25.0 36.0 94-95 31.364650787286187 35.0 33.0 35.0 24.5 35.5 96-97 31.280584949051608 35.0 33.0 35.0 24.0 35.0 98-99 31.201455180337714 35.0 33.0 35.0 24.0 35.0 100-101 31.125289175062168 35.0 33.0 35.0 23.5 35.0 102-103 30.025989704235002 34.0 30.5 35.0 19.5 35.0 104-105 30.846884389311533 35.0 32.0 35.0 21.0 35.0 106-107 30.93483945906777 35.0 33.0 35.0 20.0 35.0 108-109 30.895206337847096 35.0 33.0 35.0 20.0 35.0 110-111 30.815527078077714 35.0 33.0 35.0 19.0 35.0 112-113 30.734699029580096 35.0 33.0 35.0 18.5 35.0 114-115 30.620369647811476 35.0 33.0 35.0 17.5 35.0 116-117 30.51055252979856 35.0 32.0 35.0 11.0 35.0 118-119 30.367953847066715 35.0 32.0 35.0 2.0 35.0 120-121 30.21578222461166 35.0 32.0 35.0 2.0 35.0 122-123 30.03307177367298 35.0 32.0 35.0 2.0 35.0 124-125 29.179784986451523 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5079836366872641 1101 2 0.5286937707490935 1101 3 0.5327072520072669 1101 4 0.61831900745225 1101 5 0.526254772204588 1101 6 0.5305654508998074 1101 7 0.6066867405459462 1101 8 0.5490272062331343 1101 9 0.6299776569561288 1101 10-11 0.6251463856337338 1101 12-13 0.6765526267207562 1101 14-15 0.7383665136322293 1101 16-17 0.6752436542186473 1101 18-19 0.7610705745000388 1101 20-21 0.7554813274227712 1101 22-23 0.7999001250434858 1101 24-25 0.7343702945196426 1101 26-27 0.7001080694620043 1101 28-29 0.8142818869467021 1101 30-31 0.8022852350419143 1101 32-33 0.7684251252804302 1101 34-35 0.8516770319240621 1101 36-37 0.808272935195987 1101 38-39 0.7878261947028591 1101 40-41 0.8198652505822253 1101 42-43 0.769574118882538 1101 44-45 0.7620844833766398 1101 46-47 0.7456631048894593 1101 48-49 0.6140363852714401 1101 50-51 0.7277410748687387 1101 52-53 0.816106743218981 1101 54-55 0.7876839688382304 1101 56-57 0.7743111336746225 1101 58-59 0.674639882382472 1101 60-61 0.7800052208836448 1101 62-63 0.8434113855092562 1101 64-65 0.7772233543250877 1101 66-67 0.7143470630457003 1101 68-69 0.7013266222260057 1101 70-71 0.7770108300556515 1101 72-73 0.7317634890049192 1101 74-75 0.7286434570509144 1101 76-77 0.7310496331675793 1101 78-79 0.6404636605629221 1101 80-81 0.5976230881296303 1101 82-83 0.5911070017140858 1101 84-85 0.5996606874187975 1101 86-87 0.6051173288022476 1101 88-89 0.6491438237235236 1101 90-91 0.6186176723178249 1101 92-93 0.5832080033975195 1101 94-95 0.5829500970851953 1101 96-97 0.6087958115985579 1101 98-99 0.6431307765106347 1101 100-101 0.6341997976372049 1101 102-103 0.7119403333419001 1101 104-105 0.6694803896812083 1101 106-107 0.659403119746198 1101 108-109 0.6799964168852242 1101 110-111 0.686283709902682 1101 112-113 0.6516662004404026 1101 114-115 0.6212395272569147 1101 116-117 0.566517245204416 1101 118-119 0.5769142023638913 1101 120-121 0.6609078641939625 1101 122-123 0.6996289406589717 1101 124-125 0.735869365538889 1102 1 0.4513562883676272 1102 2 0.4390036941764599 1102 3 0.4222305409976599 1102 4 0.4185498495632558 1102 5 0.4294021196711242 1102 6 0.473184775052232 1102 7 0.42671231175003754 1102 8 0.5728072692972077 1102 9 0.5806574772130517 1102 10-11 0.5422496316295167 1102 12-13 0.5485791247635134 1102 14-15 0.6676372282414746 1102 16-17 0.6894115616706955 1102 18-19 0.6603966102440637 1102 20-21 0.6760976317584166 1102 22-23 0.6426221280271491 1102 24-25 0.7694833460636588 1102 26-27 0.8024567776896561 1102 28-29 0.7701485154409156 1102 30-31 0.8109248245723961 1102 32-33 0.7094919765918704 1102 34-35 0.742271029233919 1102 36-37 0.7475221669573457 1102 38-39 0.709379549558804 1102 40-41 0.7749646345092387 1102 42-43 0.7324699366495651 1102 44-45 0.7266169991833209 1102 46-47 0.8249264272012198 1102 48-49 0.8050830839870642 1102 50-51 0.765732513539966 1102 52-53 0.7566607425965088 1102 54-55 0.7837280956127017 1102 56-57 0.8391492640487286 1102 58-59 0.8454005082226956 1102 60-61 0.8326951315362621 1102 62-63 0.7703640997476171 1102 64-65 0.7578768623407584 1102 66-67 0.8147352761448587 1102 68-69 0.7654695332296129 1102 70-71 0.8318707143887849 1102 72-73 0.8235048829427996 1102 74-75 0.7688926190463405 1102 76-77 0.7899207877721537 1102 78-79 0.8235790024539682 1102 80-81 0.7484303848513889 1102 82-83 0.6916606829878518 1102 84-85 0.6746701853462511 1102 86-87 0.6248954297445231 1102 88-89 0.7143789511981744 1102 90-91 0.7506827838186183 1102 92-93 0.7006953777510567 1102 94-95 0.7459101320476904 1102 96-97 0.7056681648759131 1102 98-99 0.7613341554231745 1102 100-101 0.8022352141524998 1102 102-103 0.8130338872946474 1102 104-105 0.7302855608915131 1102 106-107 0.802065514424033 1102 108-109 0.807499902470969 1102 110-111 0.7721528362343371 1102 112-113 0.763564618344148 1102 114-115 0.8126539430684083 1102 116-117 0.8063750672853693 1102 118-119 0.8138119424897532 1102 120-121 0.8093165501615722 1102 122-123 0.754657004278279 1102 124-125 0.8049762314108015 1103 1 0.2789797596502588 1103 2 0.3514640531467066 1103 3 0.2808662703526217 1103 4 0.2640828747482047 1103 5 0.23189193850427614 1103 6 0.289448045186127 1103 7 0.304989473010167 1103 8 0.3349100221366541 1103 9 0.41345133380454513 1103 10-11 0.449086899532837 1103 12-13 0.42707169970359615 1103 14-15 0.4862788459130911 1103 16-17 0.5283116620485515 1103 18-19 0.5026577581162144 1103 20-21 0.4983687214488839 1103 22-23 0.5554974339534269 1103 24-25 0.568027627141781 1103 26-27 0.5423368475085937 1103 28-29 0.5417338116035992 1103 30-31 0.5668580405049894 1103 32-33 0.29527409105278934 1103 34-35 0.5259621057950596 1103 36-37 0.5919437923658037 1103 38-39 0.6119718941073558 1103 40-41 0.6471213852917757 1103 42-43 0.627228043824239 1103 44-45 0.5602025910185517 1103 46-47 0.6166151150754189 1103 48-49 0.6544777321310065 1103 50-51 0.6479131694714866 1103 52-53 0.6210827655863156 1103 54-55 0.6645393808903322 1103 56-57 0.7269082260232764 1103 58-59 0.7106684768996843 1103 60-61 0.7748867483738735 1103 62-63 0.7839442442615763 1103 64-65 0.7639964730857827 1103 66-67 0.7720081926530042 1103 68-69 0.7704025122128684 1103 70-71 0.7589448150952265 1103 72-73 0.8027633617646259 1103 74-75 0.7354506020568152 1103 76-77 0.774229806264934 1103 78-79 0.7893800957112447 1103 80-81 0.8214035826140602 1103 82-83 0.7531869086179981 1103 84-85 0.7368922925593111 1103 86-87 0.7511249328843768 1103 88-89 0.693171071684521 1103 90-91 0.6731940674739292 1103 92-93 0.6885749736713187 1103 94-95 0.7074549343229535 1103 96-97 0.6434458291678276 1103 98-99 0.6470611111863107 1103 100-101 0.6767669421712199 1103 102-103 0.6968383430846323 1103 104-105 0.7114555719255371 1103 106-107 0.7085461016013959 1103 108-109 0.737544209940264 1103 110-111 0.6900052778064456 1103 112-113 0.6056511388973895 1103 114-115 0.6760628191402454 1103 116-117 0.7139750515649119 1103 118-119 0.7878333154569184 1103 120-121 0.8166999097375616 1103 122-123 0.8200780988928074 1103 124-125 0.8101271375192276 1104 1 0.29070131710917124 1104 2 0.34057271502035036 1104 3 0.38747823478993126 1104 4 0.38689429042681667 1104 5 0.35254437694798924 1104 6 0.32168212471299995 1104 7 0.3044697719868026 1104 8 0.376723367668248 1104 9 0.36488228193944394 1104 10-11 0.3631903318386236 1104 12-13 0.36577892611173723 1104 14-15 0.48095140171075457 1104 16-17 0.4454401043047653 1104 18-19 0.4736737430941247 1104 20-21 0.5096365070667161 1104 22-23 0.46948935640768497 1104 24-25 0.48969724705597173 1104 26-27 0.5681192274869602 1104 28-29 0.6254970411893552 1104 30-31 0.537131915211873 1104 32-33 -0.11215726730812747 1104 34-35 0.4583362644857871 1104 36-37 0.5633732591289586 1104 38-39 0.6297151870339661 1104 40-41 0.6625032332786134 1104 42-43 0.6396923220095729 1104 44-45 0.5997967277166083 1104 46-47 0.5238102271998031 1104 48-49 0.6002998161280431 1104 50-51 0.5732282254990722 1104 52-53 0.5676638491202155 1104 54-55 0.505889757052941 1104 56-57 0.5325595778563894 1104 58-59 0.5733540655129801 1104 60-61 0.5452450287128912 1104 62-63 0.5209242481847696 1104 64-65 0.6231410134576407 1104 66-67 0.5622685396684091 1104 68-69 0.6316529202005583 1104 70-71 0.6261972204929549 1104 72-73 0.5968166716130625 1104 74-75 0.6411635284904378 1104 76-77 0.6148063284761562 1104 78-79 0.5342709910539511 1104 80-81 0.5631660975600354 1104 82-83 0.5673262357008113 1104 84-85 0.5853414509855028 1104 86-87 0.6078317213925182 1104 88-89 0.5880502462837178 1104 90-91 0.5497730103442677 1104 92-93 0.5836187677411111 1104 94-95 0.5620796629755311 1104 96-97 0.5041152871078616 1104 98-99 0.4959915119462046 1104 100-101 0.5266720367063407 1104 102-103 0.4839880129096592 1104 104-105 0.5367792557226707 1104 106-107 0.45934071103610563 1104 108-109 0.44615411348017275 1104 110-111 0.5627945136058266 1104 112-113 0.5054656418523997 1104 114-115 0.5593832243849555 1104 116-117 0.5543619256344776 1104 118-119 0.6153753941470619 1104 120-121 0.6099254420750064 1104 122-123 0.7226390406545491 1104 124-125 0.7389928055894011 1105 1 0.3012347132261759 1105 2 0.30941609833220696 1105 3 0.36656442708373405 1105 4 0.4040885351083361 1105 5 0.38369914542087 1105 6 0.3521776169159736 1105 7 0.29376157047791907 1105 8 0.3224267978007589 1105 9 0.34183408370488166 1105 10-11 0.39685029647928616 1105 12-13 0.42664184708598185 1105 14-15 0.5170136884729146 1105 16-17 0.4945571999501439 1105 18-19 0.4245736987250339 1105 20-21 0.4830134729864568 1105 22-23 0.3930139052970887 1105 24-25 0.6015120048521325 1105 26-27 0.5943604262560669 1105 28-29 0.5379336101434689 1105 30-31 0.5300793940614312 1105 32-33 0.11870356935626347 1105 34-35 0.5234392893702804 1105 36-37 0.4319359104664855 1105 38-39 0.46433083397668184 1105 40-41 0.488902907563471 1105 42-43 0.5334410498710938 1105 44-45 0.5123268998307537 1105 46-47 0.521344558990684 1105 48-49 0.5013961833834415 1105 50-51 0.5229089850654631 1105 52-53 0.5407703197933955 1105 54-55 0.4826744443867881 1105 56-57 0.48552962401049626 1105 58-59 0.48055657827490705 1105 60-61 0.38846370036123545 1105 62-63 0.4103292826516025 1105 64-65 0.4223998254797223 1105 66-67 0.48962241172111476 1105 68-69 0.4611490406774834 1105 70-71 0.44460843145671447 1105 72-73 0.4217655863470213 1105 74-75 0.4895459943410785 1105 76-77 0.5851342161723991 1105 78-79 0.5413437233325809 1105 80-81 0.4792865470602976 1105 82-83 0.46316496711447286 1105 84-85 0.42073987671151514 1105 86-87 0.4593105440054721 1105 88-89 0.41903465700531584 1105 90-91 0.4137631771236272 1105 92-93 0.4521408179627251 1105 94-95 0.3839151398609779 1105 96-97 0.46548380227199004 1105 98-99 0.476467442877329 1105 100-101 0.4780692814824228 1105 102-103 0.4735835447121346 1105 104-105 0.4995306234317738 1105 106-107 0.47472758005094207 1105 108-109 0.574124895939395 1105 110-111 0.4963669443868888 1105 112-113 0.5041686418873361 1105 114-115 0.4914293274588495 1105 116-117 0.5669487187174802 1105 118-119 0.5201233876112958 1105 120-121 0.556458005421991 1105 122-123 0.5138883733594142 1105 124-125 0.6097184351720024 1106 1 0.2255686878098757 1106 2 0.23763283044890215 1106 3 0.3120767076761197 1106 4 0.27100509446216847 1106 5 0.24667253452302162 1106 6 0.14289949578771655 1106 7 0.255162571192713 1106 8 0.10887063901301985 1106 9 0.30403883624212824 1106 10-11 0.29354889608327994 1106 12-13 0.30257297635321123 1106 14-15 0.3824935049630085 1106 16-17 0.3049725160861101 1106 18-19 0.3371346061275773 1106 20-21 0.2821496761749245 1106 22-23 0.2809614453437135 1106 24-25 0.3427258650590872 1106 26-27 0.3757573247429775 1106 28-29 0.4027337397710724 1106 30-31 0.4795897416803001 1106 32-33 0.45697583214353443 1106 34-35 0.5209202249257245 1106 36-37 0.45131260413851493 1106 38-39 0.4970119842862104 1106 40-41 0.5209869350593692 1106 42-43 0.5094154661645689 1106 44-45 0.5206269653099724 1106 46-47 0.5709136032568125 1106 48-49 0.5700336413469884 1106 50-51 0.5183607828954493 1106 52-53 0.5096425643459455 1106 54-55 0.5891927490903299 1106 56-57 0.6700335398636881 1106 58-59 0.6473826236163092 1106 60-61 0.6785797885075411 1106 62-63 0.7085757553368239 1106 64-65 0.6663680678851946 1106 66-67 0.6259794045429175 1106 68-69 0.5811558419238452 1106 70-71 0.617430803938305 1106 72-73 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-1.8357617861494369 2216 86-87 -1.9054184142079222 2216 88-89 -1.9173837523404949 2216 90-91 -1.9968903746667905 2216 92-93 -2.1568435047479646 2216 94-95 -2.11026289737827 2216 96-97 -2.0099602446338167 2216 98-99 -2.0339100051152172 2216 100-101 -2.057978216948289 2216 102-103 -2.1989371419539125 2216 104-105 -1.9977167861050837 2216 106-107 -2.033838200752921 2216 108-109 -2.117924822238489 2216 110-111 -2.025531290342279 2216 112-113 -2.004572912221601 2216 114-115 -2.0731321100223887 2216 116-117 -2.173576417839861 2216 118-119 -2.345071808481535 2216 120-121 -2.4146709855417647 2216 122-123 -2.3813819276450623 2216 124-125 -2.7058196650727133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47445.0 3 8688.0 4 4833.0 5 4048.0 6 4669.0 7 5654.0 8 6528.0 9 7312.0 10 7897.0 11 8893.0 12 9443.0 13 9819.0 14 9958.0 15 8932.0 16 7889.0 17 7321.0 18 7043.0 19 7153.0 20 7186.0 21 7822.0 22 9605.0 23 12275.0 24 16395.0 25 21199.0 26 26335.0 27 30465.0 28 36607.0 29 45844.0 30 54573.0 31 65098.0 32 84631.0 33 111152.0 34 154200.0 35 233631.0 36 445944.0 37 847771.0 38 394450.0 39 2047.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.828197958327564 18.501838936014998 12.638489279467327 24.031473826190105 2 15.83904440564203 19.988012438449942 39.67355391458194 24.49938924132609 3 18.852939922644453 25.117431443230814 28.915519447629656 27.114109186495078 4 12.436481656357543 15.778415359571806 37.17556242589677 34.609540558173876 5 13.652922289418454 38.515532108530564 33.92582754754869 13.90571805450229 6 32.60135696943493 35.9991309264382 17.508614818210344 13.890897285916527 7 29.435370175699887 32.144793710606336 20.927785336990677 17.4920507767031 8 26.722961406517697 35.281849085894365 19.70754548836476 18.28764401922317 9 27.29266491123856 15.616308199473805 19.539520090589058 37.551506798698576 10-11 25.21935470806224 26.433037871051546 27.57098110063557 20.776626320250642 12-13 25.40354713177709 23.829664043588462 27.88064093061624 22.88614789401821 14-15 22.848949757424954 25.599278602270203 25.065768969050623 26.486002671254223 16-17 22.920027166608428 28.91663839071288 25.652758753900862 22.510575688777827 18-19 22.80483124155141 27.878025379078036 26.9042308527423 22.412912526628254 20-21 23.72100608131721 27.033377729944103 26.791947429880985 22.453668758857702 22-23 23.59317436998531 26.927607639281277 26.616431235167816 22.8627867555656 24-25 23.084434580050928 27.165935877102964 26.71634300029434 23.033286542551767 26-27 22.77481363510451 27.48521484670235 26.76013299224598 22.97983852594716 28-29 22.933585456175727 27.276323079996278 26.975890753195902 22.814200710632093 30-31 23.095456502882282 27.160194078220652 26.695644376500756 23.048705042396307 32-33 22.881201801530782 27.296369603073135 26.853019617825552 22.969408977570538 34-35 23.16959958954326 27.317212155550525 26.600793036264424 22.912395218641795 36-37 23.414179509010722 27.140073461918192 26.348175088574948 23.097571940496138 38-39 23.042066743001566 27.445195931960427 26.4703273820652 23.042409942972807 40-41 23.104896656014486 27.162720446336774 26.803958912393693 22.92842398525505 42-43 23.028933804375082 27.19579617641087 26.493117554012873 23.28215246520118 44-45 22.81956404468887 27.288993011808856 26.693455756450646 23.19798718705163 46-47 23.055373734815205 27.43545193088206 26.355084433577154 23.154089900725573 48-49 23.122192148939906 27.287519431237317 26.50011501088041 23.09017340894237 50-51 22.98908739048368 27.398971103011107 26.443693218442966 23.16824828806225 52-53 23.107977032413363 27.180725668931956 26.429236900253812 23.282060398400876 54-55 23.112845415195505 27.122907782944484 26.42406601017292 23.340180791687093 56-57 22.958162212791567 27.51753769197273 26.382385169454714 23.14191492578099 58-59 22.966918506935965 27.04153558014547 26.552571415358695 23.438974497559876 60-61 23.016593834717717 27.260337367992094 26.626273114028244 23.09679568326195 62-63 22.852835274584514 27.314117746956235 26.671653035451996 23.16139394300725 64-65 22.855365632806972 27.145862887065057 26.58507969842005 23.413691781707918 66-67 22.833310206364832 27.395574510033487 26.33612446934448 23.434990814257198 68-69 22.573154385215492 27.212412167575756 26.728757861686358 23.48567558552239 70-71 22.877045690921875 26.999849665252217 26.677280444412645 23.445824199413263 72-73 22.790001310148227 27.28397236942566 26.37700644683283 23.549019873593284 74-75 22.643273391362985 27.491314615026987 26.292372816276394 23.573039177333634 76-77 22.74937563714644 27.32235697835482 26.45939735207271 23.468870032426032 78-79 22.911214580034674 27.213346888782315 26.588226492614314 23.287212038568697 80-81 22.7446976019972 27.655969384261027 26.37909690508763 23.220236108654145 82-83 22.928766259404185 27.380524944864614 26.38829584910297 23.302412946628227 84-85 22.993423501495773 27.16688723245707 26.287617549761016 23.55207171628614 86-87 22.75334583848409 27.073729679882835 26.679297085740984 23.49362739589209 88-89 22.821105185882224 27.20225724840754 26.63275951725586 23.343878048454375 90-91 22.930715778459298 27.29402183495732 26.55226125061062 23.22300113597276 92-93 22.793237209295604 26.92732236527857 26.541348439158657 23.738091986267168 94-95 22.873107305625513 26.902720219491943 26.65285087160722 23.571321603275326 96-97 22.907777911241446 26.983186287481338 26.55426818309489 23.554767618182325 98-99 22.76082004062249 26.898435281081177 26.60237028942578 23.738374388870543 100-101 22.623080641104647 26.92624703823398 26.678905834302157 23.77176648635922 102-103 22.829347182048185 26.70684050496345 26.603989749948276 23.859822563040094 104-105 22.59219874161604 27.022775485886058 26.590844669922458 23.794181102575447 106-107 22.714428883222713 26.82353986189704 26.651285785238287 23.810745469641954 108-109 22.65029884350156 27.04123355981556 26.4346086071912 23.87385898949168 110-111 22.62897182133737 27.158660097105773 26.43117050871353 23.781197572843325 112-113 22.62246264140134 27.31379127804796 26.587560962438488 23.476185118112213 114-115 22.46483847251451 27.506311367540924 26.221878769370466 23.8069713905741 116-117 22.439227348424573 27.35882617867721 26.513533104377597 23.68841336852062 118-119 22.563523247430066 27.392657648958306 26.32188532528842 23.72193377832321 120-121 22.688198188661193 27.550747221516687 26.33607643940809 23.42497815041403 122-123 22.545327218073027 27.730567380727685 26.212117764628935 23.511987636570357 124-125 22.70869608169462 27.80624781131914 26.010621976369563 23.474434130616682 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11849.0 1 6351.5 2 952.5 3 1009.0 4 871.0 5 739.0 6 657.0 7 617.5 8 607.5 9 583.5 10 540.0 11 518.5 12 555.0 13 572.0 14 619.5 15 712.5 16 807.0 17 922.0 18 1147.5 19 1446.5 20 1806.5 21 2513.5 22 3341.5 23 4573.5 24 6274.5 25 8670.5 26 12014.0 27 15809.0 28 19170.5 29 22845.5 30 27859.5 31 33393.5 32 39503.5 33 46046.5 34 51720.5 35 57646.0 36 66812.0 37 74604.0 38 78237.0 39 80874.0 40 82034.0 41 84140.5 42 86747.5 43 89396.0 44 93195.5 45 97076.0 46 99685.5 47 101431.0 48 101280.5 49 100317.0 50 99231.0 51 95523.5 52 93727.5 53 96441.5 54 99807.5 55 94972.0 56 85260.0 57 78058.0 58 74463.5 59 69444.5 60 59611.0 61 49393.5 62 40380.5 63 31960.5 64 25485.0 65 20298.0 66 14683.5 67 11660.0 68 8621.5 69 4931.5 70 3194.5 71 2226.5 72 1678.0 73 1352.0 74 1027.0 75 834.5 76 552.0 77 271.0 78 136.0 79 76.0 80 48.0 81 37.5 82 29.5 83 22.5 84 17.0 85 11.0 86 15.0 87 16.5 88 13.5 89 12.0 90 12.0 91 10.0 92 8.0 93 7.5 94 5.0 95 3.0 96 3.0 97 2.5 98 2.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4718862323361821 2 0.4633993285996069 3 0.4587603007096993 4 0.45516415505860813 5 0.46419048064284696 6 0.5247136119507112 7 0.5195351622131399 8 0.529280716927597 9 0.5343153208391246 10-11 0.5527635480292223 12-13 0.5616999699721837 14-15 0.5398893465983159 16-17 0.534369263023891 18-19 0.544114817738348 20-21 0.5449419312380991 22-23 0.5553347921697525 24-25 0.5485200961609347 26-27 0.5567193082454226 28-29 0.5360954129364147 30-31 0.5417953037933942 32-33 0.5432697235103416 34-35 0.5414177085000297 36-37 0.4893275387439743 38-39 0.4563329023952128 40-41 0.4538695426242153 42-43 0.4671033586202309 44-45 0.47059161990178927 46-47 0.4755722816285505 48-49 0.48999282568942604 50-51 0.47220988544478026 52-53 0.47165248286886113 54-55 0.460953949556865 56-57 0.4445015832031229 58-59 0.46257221509985597 60-61 0.4581129944925029 62-63 0.47505084050914226 64-65 0.4745833415745005 66-67 0.4827106307459665 68-69 0.49252810837344535 70-71 0.4889679241788651 72-73 0.4995945345778395 74-75 0.48930955801571874 76-77 0.48670235241867765 78-79 0.4979942497631039 80-81 0.49200666725403713 82-83 0.49276185784076626 84-85 0.4901186907872143 86-87 0.4805169818988008 88-89 0.4851919712452194 90-91 0.47048373553225653 92-93 0.47041181261923465 94-95 0.4642084613711024 96-97 0.45410329209153627 98-99 0.4171349147983191 100-101 0.41368261497327163 102-103 0.40305600457429724 104-105 0.40744330226862846 106-107 0.4063464778450457 108-109 0.41943644801501745 110-111 0.44342273950779554 112-113 0.4441060071815029 114-115 0.43655410131421146 116-117 0.42981132821841544 118-119 0.4720480588904812 120-121 0.4663481680335017 122-123 0.4295236365663282 124-125 0.4405098615304117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2780755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.90262567485912 #Duplication Level Percentage of deduplicated Percentage of total 1 80.39485277602188 44.138885081473376 2 10.382652179170503 11.400697322105168 3 3.679334339059539 6.060153478501231 4 1.7005461612009738 3.7345779732494275 5 0.9560022285862826 2.6243516250201884 6 0.5871778025207253 1.9342561857829037 7 0.3946586962954816 1.5167459070427114 8 0.29107628970613875 1.2784682061250388 9 0.22761026273039947 1.1246760948999113 >10 1.188772386228428 12.285601018215484 >50 0.12110315696780768 4.653294102577242 >100 0.07324676406937274 7.0665347892624855 >500 0.0027815226023416434 1.011570211462474 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.8543484015610956E-4 1.170188004282375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11494 0.413340981136418 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.19229130218232E-5 0.0 12-13 0.0 0.0 0.0 7.19229130218232E-5 0.0 14-15 0.0 0.0 0.0 8.990364127727901E-5 0.0 16-17 0.0 0.0 0.0 1.0788436953273481E-4 0.0 18-19 0.0 0.0 0.0 1.0788436953273481E-4 0.0 20-21 0.0 0.0 0.0 2.876916520872929E-4 0.0 22-23 0.0 0.0 0.0 5.214411194082182E-4 0.0 24-25 0.0 0.0 0.0 7.372098584736879E-4 0.0 26-27 0.0 0.0 0.0 0.0011687473366046273 0.0 28-29 0.0 0.0 0.0 0.003164608172960221 0.0 30-31 0.0 0.0 0.0 0.00920613286679337 0.0 32-33 0.0 0.0 0.0 0.022224180123743373 0.0 34-35 0.0 0.0 0.0 0.043405478008670305 0.0 36-37 0.0 0.0 0.0 0.07634617217266534 0.0 38-39 0.0 0.0 0.0 0.1275732669724589 0.0 40-41 0.0 0.0 0.0 0.1855071734115375 0.0 42-43 0.0 0.0 0.0 0.2403843560471886 0.0 44-45 0.0 0.0 0.0 0.31178582794960363 0.0 46-47 0.0 0.0 0.0 0.4000712036838916 0.0 48-49 0.0 0.0 0.0 0.4986055945237894 0.0 50-51 0.0 0.0 0.0 0.6117942788918836 0.0 52-53 0.0 0.0 0.0 0.7214407597936532 0.0 54-55 0.0 0.0 0.0 0.8420914463877616 0.0 56-57 0.0 0.0 0.0 0.983330066834367 0.0 58-59 0.0 0.0 0.0 1.1469007517742484 0.0 60-61 0.0 0.0 0.0 1.3287937988064393 0.0 62-63 0.0 0.0 0.0 1.5118016509904684 0.0 64-65 0.0 0.0 0.0 1.702828907976431 0.0 66-67 0.0 0.0 0.0 1.9240098462467927 0.0 68-69 0.0 0.0 0.0 2.1813859904953867 0.0 70-71 0.0 0.0 0.0 2.476305895341373 0.0 72-73 0.0 0.0 0.0 2.7677195581775456 0.0 74-75 0.0 0.0 0.0 3.057928512220602 0.0 76-77 0.0 0.0 0.0 3.380862391688588 0.0 78-79 0.0 0.0 0.0 3.754358079010916 0.0 80-81 0.0 0.0 0.0 4.176455674807741 0.0 82-83 0.0 0.0 0.0 4.615347270795162 0.0 84-85 0.0 0.0 0.0 5.045284464111365 0.0 86-87 0.0 0.0 0.0 5.493472096606856 0.0 88-89 0.0 0.0 0.0 5.997202198683452 0.0 90-91 0.0 0.0 0.0 6.559585436329343 0.0 92-93 0.0 0.0 0.0 7.149371303836547 0.0 94-95 0.0 0.0 0.0 7.746205616819893 0.0 96-97 0.0 0.0 0.0 8.331837936100088 0.0 98-99 0.0 0.0 0.0 8.958052759052848 0.0 100-101 0.0 0.0 0.0 9.656478186679518 0.0 102-103 0.0 0.0 0.0 10.405087826867163 0.0 104-105 0.0 0.0 0.0 11.146127580459265 0.0 106-107 0.0 0.0 0.0 11.864439693536468 0.0 108-109 0.0 0.0 0.0 12.612941449354583 0.0 110-111 0.0 0.0 0.0 13.422739507795544 0.0 112-113 0.0 0.0 0.0 14.287163018676583 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3490 0.0 60.881977 1 GTATCAA 5975 0.0 51.698196 1 ATCAACG 7170 0.0 41.925007 3 TCAACGC 7180 0.0 41.789757 4 CAACGCA 7255 0.0 41.35775 5 AACGCAG 7420 0.0 40.119305 6 GTACATG 15075 0.0 38.92831 1 TATCAAC 8200 0.0 37.01575 2 TACATGG 15760 0.0 36.44193 2 ACATGGG 15810 0.0 34.826576 3 ACGCAGA 9110 0.0 32.41769 7 CGCAGAG 9175 0.0 31.798655 8 CATGGGA 8590 0.0 31.534216 4 CATGGGG 7125 0.0 28.158455 4 AGTACAT 10570 0.0 27.25214 2 GAGTACA 11260 0.0 26.375948 1 GCAGAGT 10445 0.0 26.051126 9 ATGGGAG 3985 0.0 25.397114 5 GGACCGA 690 0.0 23.297108 6 CAGAGTA 9885 0.0 22.407066 10-11 >>END_MODULE