FastQCFastQC Report
Thu 26 May 2016
SRR1364087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780755
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95970.34512209813521866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89070.32030869314268967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.18922918416041687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60000.074.998921
GTATCAA108750.056.121591
ATCAACG145200.041.7929843
TCAACGC147100.041.2531744
CAACGCA149750.040.483425
TATCAAC150600.040.295162
TGGTATC24200.039.8269732
AACGCAG154150.039.5980386
GTGGTAT26600.037.6432341
GTACATG138600.034.9182171
ACGCAGA180700.033.5525057
CGCAGAG181000.033.496898
TACATGG145900.032.86682
ACATGGG144800.032.0887153
GCAGAGT198350.030.1169099
CATGGGA78850.028.0679744
GAGTACT127850.027.643612-13
CAGAGTA195750.026.32237410-11
GTACTTT134850.026.11804214-15
AGAGTAC188800.025.98349410-11
Produced by FastQC (version 0.11.5)