FastQCFastQC Report
Thu 26 May 2016
SRR1364086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781265
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114650.41222249587867393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.051.355711
GTATCAA48550.045.2280851
TCAACGC50700.042.737174
ATCAACG50800.042.5289423
CAACGCA52600.041.0839775
AACGCAG54550.039.39856
TATCAAC59550.036.375282
GTACATG100100.034.8365021
TACATGG99600.033.6981852
ACATGGG99700.031.7583663
ACGCAGA66550.031.4909277
CGCAGAG67150.031.032228
CATGGGA59250.025.8200514
GAGTACA71150.024.505511
CATGGGG44750.024.4758134
GCAGAGT82400.023.9884229
GTATAGC17300.022.3582741
CAGAGTA75800.021.63969210-11
AGTACAT77150.021.2892272
AGAGTAC70200.020.86395510-11
Produced by FastQC (version 0.11.5)