##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2781265 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97655527251089 31.0 31.0 34.0 28.0 34.0 2 31.029897906168596 33.0 31.0 34.0 27.0 34.0 3 31.123375154830626 33.0 31.0 34.0 27.0 34.0 4 34.59342457478881 37.0 35.0 37.0 32.0 37.0 5 34.6486631083338 37.0 35.0 37.0 32.0 37.0 6 34.685024260543315 37.0 35.0 37.0 32.0 37.0 7 34.58874397081903 37.0 35.0 37.0 31.0 37.0 8 34.54659300713884 37.0 35.0 37.0 31.0 37.0 9 36.060882008726246 39.0 37.0 39.0 31.0 39.0 10-11 35.97922779742312 39.0 37.0 39.0 30.0 39.0 12-13 35.88302714771875 39.0 37.0 39.0 30.0 39.0 14-15 37.13411181602616 40.0 37.0 41.0 31.0 41.0 16-17 37.13644924162207 40.0 37.0 41.0 31.0 41.0 18-19 37.15073051291409 40.0 37.0 41.0 31.0 41.0 20-21 37.16684548218167 40.0 37.0 41.0 31.0 41.0 22-23 37.1304199707687 40.0 37.0 41.0 31.0 41.0 24-25 37.14213909857565 40.0 37.0 41.0 31.0 41.0 26-27 37.10772741899819 40.0 37.0 41.0 31.0 41.0 28-29 37.06866353979214 40.0 37.0 41.0 30.0 41.0 30-31 37.011145827528125 40.0 37.0 41.0 30.0 41.0 32-33 36.908284180040376 40.0 37.0 41.0 30.0 41.0 34-35 36.88683872266756 40.0 37.0 41.0 30.0 41.0 36-37 36.8242378917507 40.0 37.0 41.0 30.0 41.0 38-39 36.74106692458288 40.0 37.0 41.0 30.0 41.0 40-41 36.66694813331344 40.0 36.5 41.0 30.0 41.0 42-43 36.55441139193856 40.0 36.0 41.0 30.0 41.0 44-45 36.466213395703036 40.0 36.0 41.0 30.0 41.0 46-47 36.365465894116525 40.0 36.0 41.0 29.5 41.0 48-49 36.2581312819886 40.0 36.0 41.0 29.0 41.0 50-51 36.1456673492098 40.0 35.5 41.0 29.0 41.0 52-53 36.01329646761456 40.0 35.0 41.0 28.0 41.0 54-55 35.8499626968304 39.0 35.0 41.0 28.0 41.0 56-57 35.6776393116082 39.0 35.0 41.0 28.0 41.0 58-59 35.48459190332457 39.0 35.0 41.0 28.0 41.0 60-61 35.26050538154401 39.0 35.0 41.0 27.5 41.0 62-63 35.01652018056532 38.0 34.0 40.0 27.0 41.0 64-65 34.742429613862754 38.0 34.0 40.0 26.0 41.0 66-67 34.446180964417266 37.0 34.0 40.0 26.0 41.0 68-69 34.12481227067539 37.0 34.0 39.5 26.0 41.0 70-71 33.77586224254071 36.0 33.5 39.0 26.0 41.0 72-73 33.42697711293242 36.0 33.0 39.0 26.0 40.5 74-75 33.14892162379349 35.0 33.0 38.5 25.5 40.0 76-77 32.911070681865986 35.0 33.0 37.0 26.0 39.5 78-79 32.59542168761337 35.0 33.0 37.0 25.0 39.0 80-81 32.370907842294784 35.0 33.0 36.5 25.5 39.0 82-83 32.23950162965413 35.0 33.0 36.0 25.5 38.0 84-85 32.01001019320345 35.0 33.0 36.0 25.0 37.0 86-87 31.803418588304243 35.0 33.0 35.5 25.0 37.0 88-89 31.605155208151686 35.0 33.0 35.0 24.5 36.0 90-91 31.44960206956187 35.0 33.0 35.0 24.0 36.0 92-93 31.322924280857812 35.0 33.0 35.0 24.0 36.0 94-95 31.210283629930984 35.0 33.0 35.0 23.5 36.0 96-97 31.113180153635128 35.0 33.0 35.0 23.0 35.5 98-99 31.030138623971467 35.0 33.0 35.0 23.0 35.0 100-101 30.951196487929053 35.0 33.0 35.0 20.0 35.0 102-103 29.868676123993936 34.0 30.0 35.0 18.5 35.0 104-105 30.671390895869326 35.0 32.0 35.0 19.0 35.0 106-107 30.760211090996364 35.0 32.5 35.0 19.0 35.0 108-109 30.739440865936903 35.0 33.0 35.0 19.0 35.0 110-111 30.656531470392068 35.0 32.5 35.0 18.0 35.0 112-113 30.572219475670245 35.0 32.0 35.0 16.0 35.0 114-115 30.46829680019703 35.0 32.0 35.0 8.5 35.0 116-117 30.364694842095233 35.0 32.0 35.0 3.5 35.0 118-119 30.22396391570023 35.0 32.0 35.0 2.0 35.0 120-121 30.076000668760436 35.0 32.0 35.0 2.0 35.0 122-123 29.90878934585521 35.0 32.0 35.0 2.0 35.0 124-125 29.100191279867254 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24879965216025823 1101 2 0.3112449966802444 1101 3 0.38225789964962686 1101 4 0.3790695044827004 1101 5 0.34183835459796086 1101 6 0.3486987442545697 1101 7 0.30158841270876025 1101 8 0.32868415538467843 1101 9 0.3188852740004222 1101 10-11 0.3720022661196154 1101 12-13 0.47147473445605925 1101 14-15 0.5576405511922147 1101 16-17 0.4291992282431565 1101 18-19 0.41726872792015257 1101 20-21 0.385712579448132 1101 22-23 0.40020278347298444 1101 24-25 0.37968665843665406 1101 26-27 0.4043299751005449 1101 28-29 0.47702240036974786 1101 30-31 0.4181164053064421 1101 32-33 0.3759469466989884 1101 34-35 0.3987937205896017 1101 36-37 0.4380462480912186 1101 38-39 0.4835321764609759 1101 40-41 0.5119899362921245 1101 42-43 0.4077835498503646 1101 44-45 0.5288753325285569 1101 46-47 0.5685194557727868 1101 48-49 0.38492018127153216 1101 50-51 0.41291058265126424 1101 52-53 0.49735726004310976 1101 54-55 0.5003565727254653 1101 56-57 0.51971329516374 1101 58-59 0.4470947477209748 1101 60-61 0.43243212983284707 1101 62-63 0.434546758702254 1101 64-65 0.5118912856884066 1101 66-67 0.5724255816391164 1101 68-69 0.5701155382207546 1101 70-71 0.5509437211132067 1101 72-73 0.5957968544797865 1101 74-75 0.5709243347998054 1101 76-77 0.5014589638241276 1101 78-79 0.5703778182043706 1101 80-81 0.5062849573423875 1101 82-83 0.5413249272627851 1101 84-85 0.5556946545126991 1101 86-87 0.5549004259414794 1101 88-89 0.5853703409588995 1101 90-91 0.5995110021726511 1101 92-93 0.5810316263172659 1101 94-95 0.5850829004469453 1101 96-97 0.5537814493597466 1101 98-99 0.5673871894058493 1101 100-101 0.5556388295779122 1101 102-103 0.6559328716254491 1101 104-105 0.6676125343237551 1101 106-107 0.6884196191139615 1101 108-109 0.6958814582298558 1101 110-111 0.7098986774771561 1101 112-113 0.7647442213005746 1101 114-115 0.8061986729346167 1101 116-117 0.8002564072506502 1101 118-119 0.8056287040137562 1101 120-121 0.8310687839088828 1101 122-123 0.9028655017564944 1101 124-125 1.0227659055687148 1102 1 0.39836840347592073 1102 2 0.49291084791698836 1102 3 0.4224359261041606 1102 4 0.49965693122616983 1102 5 0.4687325470387833 1102 6 0.4560942290476788 1102 7 0.44678412069577433 1102 8 0.44083644821661494 1102 9 0.4925439718821991 1102 10-11 0.4813774914041531 1102 12-13 0.4947590634950174 1102 14-15 0.6113632851541198 1102 16-17 0.6335491303435035 1102 18-19 0.5635544526419096 1102 20-21 0.6349725647800071 1102 22-23 0.6428430077818987 1102 24-25 0.6664085196162048 1102 26-27 0.6711378841620004 1102 28-29 0.6566957750288793 1102 30-31 0.7015953705787368 1102 32-33 0.6285525576898934 1102 34-35 0.7197906725798475 1102 36-37 0.6563234208915603 1102 38-39 0.7133159225236128 1102 40-41 0.6276502999578497 1102 42-43 0.5778786667868587 1102 44-45 0.6636747896348609 1102 46-47 0.6127012992083181 1102 48-49 0.6668872769921066 1102 50-51 0.6751815613882428 1102 52-53 0.6838446811154171 1102 54-55 0.6939217733164185 1102 56-57 0.6638149012563019 1102 58-59 0.6962374649385268 1102 60-61 0.6897729215464068 1102 62-63 0.7344273999473714 1102 64-65 0.6600708423957045 1102 66-67 0.6790969117959591 1102 68-69 0.6810706389511765 1102 70-71 0.7217792131731002 1102 72-73 0.6971756199156829 1102 74-75 0.643370316973126 1102 76-77 0.7010545810725333 1102 78-79 0.6515549882171783 1102 80-81 0.6179762278847392 1102 82-83 0.629082448522432 1102 84-85 0.623391998966639 1102 86-87 0.6231195894737844 1102 88-89 0.5994413131596268 1102 90-91 0.6766318488868812 1102 92-93 0.6189123511880084 1102 94-95 0.6590268145682394 1102 96-97 0.6383380076751628 1102 98-99 0.6806431962727615 1102 100-101 0.6686830088981601 1102 102-103 0.7124919079506888 1102 104-105 0.6856665372516133 1102 106-107 0.7530444428513334 1102 108-109 0.7239102196767142 1102 110-111 0.7184131115479673 1102 112-113 0.7885518112389711 1102 114-115 0.758958847145049 1102 116-117 0.8448948273607861 1102 118-119 0.8192844317441086 1102 120-121 0.8479883139521078 1102 122-123 0.8027751236052723 1102 124-125 0.8677111452041828 1103 1 0.20470304635171388 1103 2 0.220848026738647 1103 3 0.3177344741399999 1103 4 0.2697416163370079 1103 5 0.3282772071464706 1103 6 0.3036917459340245 1103 7 0.28593693170698486 1103 8 0.24861860137472291 1103 9 0.3460973984500413 1103 10-11 0.3740394205782849 1103 12-13 0.37011882931319207 1103 14-15 0.4430654497387181 1103 16-17 0.3978479978298921 1103 18-19 0.4399009827639446 1103 20-21 0.46928600719751046 1103 22-23 0.490233188977939 1103 24-25 0.44198683261016214 1103 26-27 0.4134242285726941 1103 28-29 0.5139251865948324 1103 30-31 0.40731883391410406 1103 32-33 0.19812328066176832 1103 34-35 0.41593253933825025 1103 36-37 0.4467845968995974 1103 38-39 0.5289557143892907 1103 40-41 0.6293143608500387 1103 42-43 0.5428335597555645 1103 44-45 0.5642957216318294 1103 46-47 0.6001125672119656 1103 48-49 0.5792434090603322 1103 50-51 0.5935607952598332 1103 52-53 0.5300588082014457 1103 54-55 0.5595240911758594 1103 56-57 0.6136653891862878 1103 58-59 0.5765947888030141 1103 60-61 0.5947486709497483 1103 62-63 0.5990060938246771 1103 64-65 0.5886141325520171 1103 66-67 0.47419050466238133 1103 68-69 0.5699874712304265 1103 70-71 0.5859393961199686 1103 72-73 0.49045233780707065 1103 74-75 0.44437720051804774 1103 76-77 0.45701663403374937 1103 78-79 0.4389856075519205 1103 80-81 0.4567980697014278 1103 82-83 0.41794790762508427 1103 84-85 0.3089010004072392 1103 86-87 0.32796763545009 1103 88-89 0.4030049529299866 1103 90-91 0.42208812562934384 1103 92-93 0.3849513026530289 1103 94-95 0.38844222007077533 1103 96-97 0.44322283653874806 1103 98-99 0.4579938454638466 1103 100-101 0.4888818822206602 1103 102-103 0.41715488762564945 1103 104-105 0.4724728457855498 1103 106-107 0.5192427356339024 1103 108-109 0.5122220156952544 1103 110-111 0.47727671230850177 1103 112-113 0.46532967638783873 1103 114-115 0.5057808517586544 1103 116-117 0.46278782064310775 1103 118-119 0.5053190675551278 1103 120-121 0.44707250452893277 1103 122-123 0.41754502706363184 1103 124-125 0.5152604373447787 1104 1 0.3442714925316359 1104 2 0.35427852289574346 1104 3 0.36667691511685163 1104 4 0.34240362722728435 1104 5 0.41590849056599666 1104 6 0.3671414855576529 1104 7 0.38007814411963636 1104 8 0.3527653471379111 1104 9 0.43826745497456443 1104 10-11 0.44422137365796743 1104 12-13 0.5023718177828513 1104 14-15 0.5355469540021929 1104 16-17 0.5126840600683522 1104 18-19 0.6270653618866717 1104 20-21 0.5545803039258814 1104 22-23 0.5280104393927871 1104 24-25 0.5821200488412046 1104 26-27 0.5902674090225943 1104 28-29 0.6854449452302589 1104 30-31 0.6300373812120768 1104 32-33 -0.1008443321593262 1104 34-35 0.44867845043575727 1104 36-37 0.47640097205303533 1104 38-39 0.5136965572584771 1104 40-41 0.5311579793939813 1104 42-43 0.6576579790879009 1104 44-45 0.621841557406853 1104 46-47 0.6370059369036412 1104 48-49 0.4861161393247926 1104 50-51 0.5017941416716383 1104 52-53 0.44231914477053635 1104 54-55 0.5236669104283465 1104 56-57 0.5194465970011777 1104 58-59 0.5339380914737291 1104 60-61 0.5260723951233643 1104 62-63 0.5502947882955098 1104 64-65 0.5187448152629273 1104 66-67 0.4769905938850627 1104 68-69 0.5353083921961783 1104 70-71 0.5241105450579866 1104 72-73 0.517585239501436 1104 74-75 0.5103633413015771 1104 76-77 0.5717946005467311 1104 78-79 0.4741227993007868 1104 80-81 0.5366740786914193 1104 82-83 0.46788228262229836 1104 84-85 0.4150858190674924 1104 86-87 0.432127925002046 1104 88-89 0.4169034642121723 1104 90-91 0.37491671324087505 1104 92-93 0.4241478292652374 1104 94-95 0.41691815474813154 1104 96-97 0.4717077707073223 1104 98-99 0.48795650797074686 1104 100-101 0.4542322074646492 1104 102-103 0.46323908425176086 1104 104-105 0.534374524413419 1104 106-107 0.5060136023502508 1104 108-109 0.510021565839498 1104 110-111 0.508435356705931 1104 112-113 0.5095660962892481 1104 114-115 0.5289516903181735 1104 116-117 0.5346368180908776 1104 118-119 0.5677559337481384 1104 120-121 0.5983037864039886 1104 122-123 0.5603923769807793 1104 124-125 0.6341518841748659 1105 1 0.22747180610398132 1105 2 0.2610488239396247 1105 3 0.23424808810345965 1105 4 0.13303150534768093 1105 5 0.24305233706944307 1105 6 0.21829429676738243 1105 7 0.11042787106641327 1105 8 0.14475572594965058 1105 9 0.24961341135819026 1105 10-11 0.3223788295378043 1105 12-13 0.334700248742827 1105 14-15 0.3600974862816315 1105 16-17 0.3383847283333381 1105 18-19 0.33799404341596784 1105 20-21 0.3391454327093726 1105 22-23 0.3104053902528321 1105 24-25 0.42009795209067136 1105 26-27 0.38581914244762316 1105 28-29 0.43243201157341105 1105 30-31 0.46397238949624864 1105 32-33 0.10269616171711249 1105 34-35 0.4314080447876094 1105 36-37 0.3872974607160984 1105 38-39 0.41574797326364177 1105 40-41 0.6045587664509426 1105 42-43 0.506616234769595 1105 44-45 0.4627053361940696 1105 46-47 0.5088610756185261 1105 48-49 0.5339624936855856 1105 50-51 0.5544967793825606 1105 52-53 0.5732404979891044 1105 54-55 0.5658180732190488 1105 56-57 0.6116099936822081 1105 58-59 0.5681811112427297 1105 60-61 0.5186968621826367 1105 62-63 0.5901222633888707 1105 64-65 0.5581937010470881 1105 66-67 0.5007607121720525 1105 68-69 0.5241047637505503 1105 70-71 0.49199517015387073 1105 72-73 0.49451843464491674 1105 74-75 0.5698725609056154 1105 76-77 0.5437985298198171 1105 78-79 0.4828879759377216 1105 80-81 0.5117866184590696 1105 82-83 0.5530958977699569 1105 84-85 0.5390566994026074 1105 86-87 0.5105508893668507 1105 88-89 0.5373949299464549 1105 90-91 0.5081564938845169 1105 92-93 0.5741040036826384 1105 94-95 0.5285172955502908 1105 96-97 0.5708981997772078 1105 98-99 0.5810392800079818 1105 100-101 0.5517696786383581 1105 102-103 0.5695366220836391 1105 104-105 0.6587862155639002 1105 106-107 0.6514668033611848 1105 108-109 0.6576361375751851 1105 110-111 0.5609877610411189 1105 112-113 0.5752270990955282 1105 114-115 0.6143781210521126 1105 116-117 0.6926991021191284 1105 118-119 0.7294900681658305 1105 120-121 0.7231633521640042 1105 122-123 0.6709970557975744 1105 124-125 0.7421855523220238 1106 1 0.1543734401833845 1106 2 0.226949113743796 1106 3 0.2059737495398437 1106 4 0.2506830870542771 1106 5 0.20561436723522775 1106 6 0.2160117250548126 1106 7 0.2020453939828002 1106 8 0.21322172275336015 1106 9 0.25713063950751547 1106 10-11 0.2852664002649661 1106 12-13 0.2865831765427984 1106 14-15 0.3001023143628103 1106 16-17 0.33365900676962923 1106 18-19 0.3115191105462145 1106 20-21 0.3939055306405663 1106 22-23 0.32411492778302176 1106 24-25 0.3666742854022118 1106 26-27 0.32579041733306013 1106 28-29 0.3987835844040717 1106 30-31 0.31266537173053877 1106 32-33 0.3779931239032308 1106 34-35 0.3399809847227928 1106 36-37 0.3614469155378046 1106 38-39 0.38629969039988765 1106 40-41 0.38600157056995243 1106 42-43 0.331259807373101 1106 44-45 0.350239497821093 1106 46-47 0.3225833365205375 1106 48-49 0.33887353626003147 1106 50-51 0.3246792801353422 1106 52-53 0.43380704653261404 1106 54-55 0.3406085760123432 1106 56-57 0.40665424930352145 1106 58-59 0.4655443311183447 1106 60-61 0.45556850775781044 1106 62-63 0.39765976065093156 1106 64-65 0.4279141578906973 1106 66-67 0.39762284116616087 1106 68-69 0.3983505177353024 1106 70-71 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2216 96-97 -2.325945135342156 2216 98-99 -2.4099158508685647 2216 100-101 -2.475150604745579 2216 102-103 -2.5655051512488996 2216 104-105 -2.4092744500311447 2216 106-107 -2.4516383146162326 2216 108-109 -2.6170779482734794 2216 110-111 -2.4734649190009534 2216 112-113 -2.426951960657206 2216 114-115 -2.498667710544801 2216 116-117 -2.636895766012085 2216 118-119 -2.688217756644157 2216 120-121 -2.713527624399269 2216 122-123 -2.807014741944087 2216 124-125 -2.9907172477811734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53694.0 3 9279.0 4 5075.0 5 4398.0 6 4645.0 7 5518.0 8 6403.0 9 7387.0 10 8259.0 11 9310.0 12 10253.0 13 10473.0 14 10784.0 15 9748.0 16 8469.0 17 7680.0 18 7485.0 19 7478.0 20 7839.0 21 8267.0 22 9986.0 23 12890.0 24 16713.0 25 21391.0 26 26282.0 27 31201.0 28 37639.0 29 46500.0 30 55071.0 31 65994.0 32 83988.0 33 110463.0 34 149555.0 35 219900.0 36 401157.0 37 833404.0 38 453921.0 39 2766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44191977574199 18.69317689217698 12.773169956362166 24.091733375718867 2 15.50442338506351 19.95576614936492 40.48853011846263 24.051280347108943 3 19.216871560521103 24.376433040958062 29.437605017431267 26.969090381089565 4 12.23656779275342 16.18189265214307 38.3741282736242 33.20741128147931 5 13.21691562919237 38.25363329723576 35.317447990226924 13.212003083344953 6 32.28667881101087 36.77000202405737 17.740248380754107 13.203070784177653 7 28.27773772026362 32.227926653185655 21.500620740877277 17.993714885673445 8 25.500568386162826 35.88217327735618 20.327224161020457 18.290034175460537 9 25.879282909714497 16.274605064629075 20.293616039895866 37.552495985760565 10-11 24.43443764636976 26.73392632951872 28.64149882633033 20.19013719778119 12-13 25.01118640225236 24.231383431180078 28.27575371944485 22.481676447122712 14-15 22.06557826257333 26.184960733873847 25.849669516732387 25.899791486820433 16-17 22.138994926175336 29.132931386701422 26.76316446309327 21.96490922402997 18-19 21.949254977227156 28.327892807389116 28.094985636035414 21.627866579348318 20-21 22.617460825303176 27.63908847543594 28.099911971035397 21.643538728225483 22-23 22.18328223590003 27.763197880392056 27.754176964340626 22.299342919367295 24-25 22.023372243689117 27.76392110530091 28.05307344570675 22.159633205303223 26-27 21.871776450685324 28.040165175887154 27.763190768315184 22.32486760511234 28-29 21.882684910491296 28.270096035286564 27.779653474813102 22.06756557940904 30-31 22.128836401792 27.915873229622413 27.755074005420827 22.20021636316476 32-33 21.81123486483233 27.949534317827947 27.71428013666614 22.524950680673584 34-35 22.36303149545811 28.0678224671638 27.606159007372042 21.962987030006044 36-37 22.399736384423484 27.682598318836465 27.598122822962956 22.319542473777094 38-39 22.036164699045 27.982861653763003 27.63049034893446 22.35048329825754 40-41 22.42286584859305 27.812684884660865 27.711695871369756 22.05275339537632 42-43 22.079754300934756 27.839247297359048 27.620644631320452 22.46035377038574 44-45 22.155917491040373 28.03951358173253 27.766204504406705 22.038364422820393 46-47 22.35910236060887 28.06254783474022 27.232777100090733 22.34557270456018 48-49 22.110469423780355 27.9745586138831 27.550150102082423 22.36482186025412 50-51 22.182582209174395 28.15610598797409 27.46384577327498 22.197466029576535 52-53 22.362776139768297 27.998221159638458 27.48030188987338 22.15870081071986 54-55 22.046634861550217 28.032607017173138 27.66804922297807 22.25270889829858 56-57 22.155394074094673 28.285443572510367 27.381159831433433 22.178002521961524 58-59 22.045247129678444 27.982738607077906 27.60146793723176 22.37054632601189 60-61 21.986714659567667 28.137903134047203 27.657366085538378 22.21801612084675 62-63 21.96812434655127 28.006117038763644 27.785037315670557 22.240721299014528 64-65 21.836430527057914 28.02530259483641 27.67463633255076 22.463630545554913 66-67 21.870548745867474 28.190464766706185 27.43485463970956 22.504131847716778 68-69 22.167316008126328 27.85130155850983 27.65081719464616 22.33056523871767 70-71 21.938793760358482 27.79712023771715 27.637852061977284 22.62623393994708 72-73 22.019075158190255 27.829794676539528 27.64640401630966 22.504726148960554 74-75 21.99307965478848 28.11566836902173 27.401015270610863 22.49023670557893 76-77 21.9999338801529 27.968930175454254 27.58883138228599 22.44230456210686 78-79 22.092065661349068 28.021779673051956 27.452119373364635 22.43403529223434 80-81 22.077069205830732 28.068735202537237 27.371663485485048 22.482532106146984 82-83 22.125462105288644 28.092117173026747 27.474645862308073 22.307774859376543 84-85 22.146414750196968 28.045691400578228 27.41881489465371 22.3890789545711 86-87 22.086996703080132 28.025336299013702 27.42601620406039 22.461650793845774 88-89 22.163277229205555 27.97529248135799 27.36156081106592 22.499869478370535 90-91 22.06918887206648 28.090720351948406 27.444960195192934 22.39513058079218 92-93 22.185532978053164 27.845010953187405 27.58191047864562 22.38754559011381 94-95 22.01113425605249 27.818680285597296 27.65469898802642 22.515486470323793 96-97 22.218413651042816 27.553601826765718 27.603139338201967 22.624845183989507 98-99 21.96394716024403 27.664165634950464 27.48123684629541 22.890650358510097 100-101 22.05143878288036 27.65848671679569 27.618374351626578 22.67170014869737 102-103 21.978596015588195 27.46009541361241 27.736968171997205 22.824340398802185 104-105 21.848923951488555 27.61694182070636 27.55309481956588 22.981039408239205 106-107 21.944981810968006 27.691336829319496 27.370012005681716 22.993669354030786 108-109 21.915162303343553 27.631583460684055 27.383585793165576 23.06966844280681 110-111 21.91441585821148 27.79324012586169 27.43257506331457 22.85976895261226 112-113 21.98517897334108 27.864274095454373 27.421391279726564 22.729155651477985 114-115 21.873004208034537 28.08106944204947 27.220286680816834 22.82563966909916 116-117 21.907571131871855 28.013518337196857 27.351106814760755 22.72780371617053 118-119 21.77728606909009 28.08299841950779 27.195412056897723 22.944303454504404 120-121 21.726151772090443 28.291693398298424 27.191596441947564 22.790558387663566 122-123 21.889568327917964 28.25991106707236 27.123116566845223 22.72740403816446 124-125 21.87092021083538 28.24865429029049 27.06309351621623 22.817331982657905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11644.0 1 6197.0 2 788.0 3 735.0 4 591.0 5 517.5 6 460.0 7 416.0 8 414.5 9 415.5 10 385.0 11 365.0 12 404.5 13 446.5 14 471.0 15 525.5 16 598.5 17 714.0 18 906.5 19 1201.0 20 1639.5 21 2326.5 22 3286.5 23 4588.0 24 6606.5 25 9498.0 26 13747.0 27 19323.0 28 24176.5 29 29504.0 30 36244.0 31 43446.0 32 53295.5 33 60562.0 34 65694.5 35 72000.0 36 78843.0 37 85599.0 38 88458.5 39 90789.0 40 93677.0 41 96118.5 42 97769.5 43 99131.5 44 101724.0 45 105195.5 46 105460.0 47 103994.5 48 102777.5 49 100748.0 50 98452.0 51 94635.5 52 91224.5 53 88572.0 54 85508.5 55 79652.5 56 72355.0 57 64953.0 58 58503.0 59 51453.0 60 41826.5 61 33760.0 62 27585.5 63 20280.0 64 13709.5 65 9933.5 66 7434.0 67 5924.5 68 4541.0 69 3311.5 70 2509.5 71 1760.0 72 1219.0 73 1039.0 74 861.5 75 586.5 76 387.0 77 228.5 78 141.5 79 79.0 80 46.5 81 38.0 82 29.5 83 18.5 84 12.5 85 11.0 86 11.0 87 10.5 88 10.0 89 9.0 90 9.5 91 10.0 92 6.0 93 5.0 94 4.5 95 2.5 96 2.5 97 1.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46831208101349564 2 0.4601143724168678 3 0.45579978894495843 4 0.4537863166580674 5 0.4617682960810998 6 0.522963471657681 7 0.5181095652517829 8 0.5270982808182607 9 0.5343971178582407 10-11 0.5525363458713931 12-13 0.5619924746473277 14-15 0.5397903471981275 16-17 0.5325454424515463 18-19 0.5444105469992971 20-21 0.5439970660832392 22-23 0.5562396966847819 24-25 0.5482756946928825 26-27 0.5567969970499035 28-29 0.5352240796903567 30-31 0.5422892101256083 32-33 0.543206059113389 34-35 0.5419296615029492 36-37 0.4889321945229958 38-39 0.45793910324978027 40-41 0.4547211430769812 42-43 0.4674311868879808 44-45 0.4729322808146653 46-47 0.4771749545620428 48-49 0.4907299376362914 50-51 0.47311205512599486 52-53 0.47350755861091987 54-55 0.46175031864996685 56-57 0.44542681118124305 58-59 0.46347615203873055 60-61 0.4602222370036656 62-63 0.47672551878371894 64-65 0.4763479927299269 66-67 0.4830715519736523 68-69 0.4932108231326393 70-71 0.4892018559899901 72-73 0.5015163963160648 74-75 0.49001084039097315 76-77 0.4876737743436889 78-79 0.5002759535678909 80-81 0.49213217726466196 82-83 0.49502654367706783 84-85 0.4909456668098868 86-87 0.4810221248244953 88-89 0.48627153471531837 90-91 0.47215925127594816 92-93 0.47176374779102315 94-95 0.46602894725961025 96-97 0.4550447368373744 98-99 0.41736404118269926 100-101 0.4151168622910798 102-103 0.4034135546235256 104-105 0.408519145065285 106-107 0.4072427474548452 108-109 0.42004267842150966 110-111 0.44413243613967024 112-113 0.44443805246893053 114-115 0.4371212379978175 116-117 0.4310808211371444 118-119 0.4724468901740755 120-121 0.4677368032172411 122-123 0.4309909339814797 124-125 0.4415257086253917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2781265.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.98165234469123 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80059976423004 56.13102639684271 2 9.870613437198823 13.222999953585788 3 2.9039411153596757 5.8353232265543005 4 1.2361253209977212 3.311908660221568 5 0.6007811174601619 2.0120655972485837 6 0.35020657583329673 1.4074449066774375 7 0.23389702384568373 1.0966766394982679 8 0.16096816768121877 0.8625531076948287 9 0.12333429472652956 0.7435021366395079 >10 0.6332630522335694 7.917954550251712 >50 0.05349719978269746 2.4828861724979423 >100 0.03198322329286194 3.712239992633864 >500 6.580894647903959E-4 0.25266609996283046 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.3161789295807917E-4 1.0107525596908065 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11465 0.41222249587867393 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0786458679773412E-4 2 0.0 0.0 0.0 0.0 1.0786458679773412E-4 3 0.0 0.0 0.0 0.0 1.0786458679773412E-4 4 0.0 0.0 0.0 3.595486226591138E-5 1.0786458679773412E-4 5 0.0 0.0 0.0 3.595486226591138E-5 1.0786458679773412E-4 6 0.0 0.0 0.0 3.595486226591138E-5 1.0786458679773412E-4 7 0.0 0.0 0.0 3.595486226591138E-5 1.0786458679773412E-4 8 0.0 0.0 0.0 3.595486226591138E-5 1.0786458679773412E-4 9 0.0 0.0 0.0 3.595486226591138E-5 1.7977431132955686E-4 10-11 0.0 0.0 0.0 3.595486226591138E-5 1.7977431132955686E-4 12-13 0.0 0.0 0.0 3.595486226591138E-5 1.9775174246251256E-4 14-15 0.0 0.0 0.0 3.595486226591138E-5 2.3370660472842394E-4 16-17 0.0 0.0 0.0 1.7977431132955686E-4 2.696614669943353E-4 18-19 0.0 0.0 0.0 1.7977431132955686E-4 2.87638898127291E-4 20-21 0.0 0.0 0.0 3.0561632926024665E-4 3.235937603932024E-4 22-23 0.0 0.0 0.0 4.314583471909365E-4 3.235937603932024E-4 24-25 0.0 0.0 0.0 6.831423830523161E-4 3.235937603932024E-4 26-27 0.0 0.0 0.0 0.0012224653170409866 3.5954862265911373E-4 28-29 0.0 0.0 0.0 0.0026966146699433534 3.955034849250251E-4 30-31 0.0 0.0 0.0 0.008647144374951685 3.955034849250251E-4 32-33 0.0 0.0 0.0 0.020745955527430863 3.955034849250251E-4 34-35 0.0 0.0 0.0 0.04102449784540488 4.134809160579808E-4 36-37 0.0 0.0 0.0 0.07318612214226261 4.314583471909365E-4 38-39 0.0 0.0 0.0 0.12167125390784408 4.314583471909365E-4 40-41 0.0 0.0 0.0 0.1733923232773576 4.314583471909365E-4 42-43 0.0 0.0 0.0 0.22425047595248926 4.314583471909365E-4 44-45 0.0 0.0 0.0 0.2908029260066912 4.853906405898036E-4 46-47 0.0 0.0 0.0 0.37179125326065654 5.393229339886706E-4 48-49 0.0 0.0 0.0 0.45732787059125973 5.393229339886706E-4 50-51 0.0 0.0 0.0 0.5499655732193804 5.75277796254582E-4 52-53 0.0 0.0 0.0 0.6415785622729225 6.831423830523161E-4 54-55 0.0 0.0 0.0 0.7454341819280075 7.190972453182275E-4 56-57 0.0 0.0 0.0 0.8708806963737724 7.370746764511832E-4 58-59 0.0 0.0 0.0 1.0128664474618563 7.550521075841388E-4 60-61 0.0 0.0 0.0 1.163499342924892 7.730295387170945E-4 62-63 0.0 0.0 0.0 1.3162535752616167 7.910069698500502E-4 64-65 0.0 0.0 0.0 1.4776909068355586 7.910069698500502E-4 66-67 0.0 0.0 0.0 1.6727999669215268 8.449392632489173E-4 68-69 0.0 0.0 0.0 1.8937246181144192 8.62916694381873E-4 70-71 0.0 0.0 0.0 2.141651370869011 8.62916694381873E-4 72-73 0.0 0.0 0.0 2.3922567608624132 9.348264189136958E-4 74-75 0.0 0.0 0.0 2.6462778627710772 0.0010067361434455185 76-77 0.0 0.0 0.0 2.9286134187141464 0.0010067361434455185 78-79 0.0 0.0 0.0 3.2597037678897913 0.0010247135745784742 80-81 0.0 0.0 0.0 3.6183175641299914 0.0010786458679773413 82-83 0.0 0.0 0.0 3.9953042949880717 0.0011865104547750754 84-85 0.0 0.0 0.0 4.379158404538941 0.0011865104547750754 86-87 0.0 0.0 0.0 4.787785414191024 0.0012404427481739425 88-89 0.0 0.0 0.0 5.230209994373064 0.0012943750415728096 90-91 0.0 0.0 0.0 5.716930964866706 0.0012943750415728096 92-93 0.0 0.0 0.0 6.219382187601685 0.0013303299038387208 94-95 0.0 3.595486226591138E-5 0.0 6.727837872335071 0.0013483073349716765 96-97 0.0 3.595486226591138E-5 0.0 7.243880033006564 0.001492126784035322 98-99 0.0 3.595486226591138E-5 0.0 7.805872507653891 0.001546059077434189 100-101 0.0 3.595486226591138E-5 0.0 8.431145539889222 0.001546059077434189 102-103 0.0 3.595486226591138E-5 0.0 9.08302517020133 0.0015820139397001005 104-105 0.0 3.595486226591138E-5 0.0 9.729134045119757 0.0016179688019660118 106-107 0.0 3.595486226591138E-5 0.0 10.376159769025964 0.0016179688019660118 108-109 0.0 3.595486226591138E-5 0.0 11.056659469701737 0.0016179688019660118 110-111 0.0 3.595486226591138E-5 0.0 11.784385881963782 0.0016539236642319232 112-113 0.0 3.595486226591138E-5 0.0 12.554844648028865 0.0016539236642319232 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2920 0.0 51.35571 1 GTATCAA 4855 0.0 45.228085 1 TCAACGC 5070 0.0 42.73717 4 ATCAACG 5080 0.0 42.528942 3 CAACGCA 5260 0.0 41.083977 5 AACGCAG 5455 0.0 39.3985 6 TATCAAC 5955 0.0 36.37528 2 GTACATG 10010 0.0 34.836502 1 TACATGG 9960 0.0 33.698185 2 ACATGGG 9970 0.0 31.758366 3 ACGCAGA 6655 0.0 31.490927 7 CGCAGAG 6715 0.0 31.03222 8 CATGGGA 5925 0.0 25.820051 4 GAGTACA 7115 0.0 24.50551 1 CATGGGG 4475 0.0 24.475813 4 GCAGAGT 8240 0.0 23.988422 9 GTATAGC 1730 0.0 22.358274 1 CAGAGTA 7580 0.0 21.639692 10-11 AGTACAT 7715 0.0 21.289227 2 AGAGTAC 7020 0.0 20.863955 10-11 >>END_MODULE