FastQCFastQC Report
Thu 26 May 2016
SRR1364086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781265
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66120.23773354930220603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.22547294126953024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.12289371922488508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.071.566971
GTATCAA88650.047.4501461
TCAACGC109300.037.7775344
ATCAACG109250.037.739013
CAACGCA111050.037.1286355
TATCAAC114850.036.107392
AACGCAG116350.035.79536
TGGTATC22250.034.2274322
GTGGTAT24850.032.368231
CGCAGAG136650.030.4407658
ACGCAGA136950.030.4175367
TAGTGCG1954.7817593E-827.4660349
TACATGG89650.026.8117662
GTACATG92500.026.4091071
GCAGAGT157450.026.1170089
GAGTACT111500.025.85778612-13
CAGAGTA151400.025.2931510-11
ACATGGG90300.024.7071253
GTACTTT119600.024.0018614-15
AGAGTAC143750.023.5964810-11
Produced by FastQC (version 0.11.5)