FastQCFastQC Report
Thu 26 May 2016
SRR1364085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423738
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142310.415656805514908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49550.046.9588931
GGTATCA33200.045.7074361
TCAACGC53100.041.9302644
ATCAACG53100.041.9222563
CAACGCA55700.039.9765365
AACGCAG59600.037.461076
GTACATG122650.037.0689741
TACATGG122800.036.2499242
TATCAAC64200.035.410512
ACATGGG124300.034.0463
ACGCAGA72600.030.0984217
CGCAGAG72800.029.361448
CATGGGA74050.027.253684
CATGGGG61050.026.0361124
GAGTACA85000.024.5738371
AGTACAT84900.022.9916442
GCAGAGT92150.021.7102389
ATGGGAT33950.020.1672575
AGAGTAC77800.019.85981810-11
CAGAGTA82050.019.70192710-11
Produced by FastQC (version 0.11.5)