##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364085_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3423738 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13087566864053 31.0 31.0 34.0 28.0 34.0 2 31.196996674395063 33.0 31.0 34.0 28.0 34.0 3 31.279160379678586 33.0 31.0 34.0 28.0 34.0 4 34.752946633182795 37.0 35.0 37.0 32.0 37.0 5 34.79927552867655 37.0 35.0 37.0 32.0 37.0 6 34.86293781825595 37.0 35.0 37.0 32.0 37.0 7 34.77734511227202 37.0 35.0 37.0 32.0 37.0 8 34.732265728277106 37.0 35.0 37.0 32.0 37.0 9 36.28515470517896 39.0 37.0 39.0 32.0 39.0 10-11 36.20536238462172 39.0 37.0 39.0 32.0 39.0 12-13 36.11700442615644 39.0 37.0 39.0 31.0 39.0 14-15 37.39641482496616 40.0 38.0 41.0 31.5 41.0 16-17 37.398508004993374 40.0 38.0 41.0 31.0 41.0 18-19 37.40286785963178 40.0 38.0 41.0 31.0 41.0 20-21 37.41790537126381 40.0 38.0 41.0 31.0 41.0 22-23 37.37114171703559 40.0 38.0 41.0 31.0 41.0 24-25 37.38108917796864 40.0 38.0 41.0 31.0 41.0 26-27 37.34292431254961 40.0 38.0 41.0 31.0 41.0 28-29 37.3013654958411 40.0 37.5 41.0 31.0 41.0 30-31 37.25699469410334 40.0 37.0 41.0 31.0 41.0 32-33 37.161492351342304 40.0 37.0 41.0 31.0 41.0 34-35 37.13919858937804 40.0 37.0 41.0 30.5 41.0 36-37 37.08983587529186 40.0 37.0 41.0 30.0 41.0 38-39 37.01761320521605 40.0 37.0 41.0 30.0 41.0 40-41 36.94974673879835 40.0 37.0 41.0 30.0 41.0 42-43 36.85028629527143 40.0 37.0 41.0 30.0 41.0 44-45 36.76289701490009 40.0 37.0 41.0 30.0 41.0 46-47 36.66234434410577 40.0 36.0 41.0 30.0 41.0 48-49 36.56704923098671 40.0 36.0 41.0 30.0 41.0 50-51 36.45250425120146 40.0 36.0 41.0 30.0 41.0 52-53 36.323533079926094 40.0 36.0 41.0 29.5 41.0 54-55 36.1614150965991 39.5 35.0 41.0 29.0 41.0 56-57 35.9899145320115 39.0 35.0 41.0 29.0 41.0 58-59 35.794042213510494 39.0 35.0 41.0 28.0 41.0 60-61 35.564466819598934 39.0 35.0 41.0 28.0 41.0 62-63 35.31432092642603 38.0 35.0 40.5 28.0 41.0 64-65 35.04429164848479 38.0 34.0 40.0 28.0 41.0 66-67 34.74037134266699 37.0 34.0 40.0 28.0 41.0 68-69 34.41859569861946 37.0 34.0 39.5 27.0 41.0 70-71 34.07827643353551 36.0 34.0 39.0 26.0 41.0 72-73 33.718498319672825 36.0 33.5 39.0 26.0 40.5 74-75 33.432356681498405 35.0 33.5 38.5 26.0 40.0 76-77 33.19385113580537 35.0 33.0 37.0 26.0 39.5 78-79 32.88428334761596 35.0 33.0 37.0 26.0 39.0 80-81 32.66291900840543 35.0 33.0 36.5 26.0 39.0 82-83 32.54756015209108 35.0 33.0 36.0 26.0 37.5 84-85 32.32652834416652 35.0 33.0 36.0 26.0 37.0 86-87 32.12623249793063 35.0 33.0 35.5 26.0 37.0 88-89 31.940366786243573 35.0 33.0 35.0 26.0 36.0 90-91 31.79723682127546 35.0 33.0 35.0 25.5 36.0 92-93 31.678085034544118 35.0 33.0 35.0 25.0 36.0 94-95 31.572946440411037 35.0 33.0 35.0 25.0 36.0 96-97 31.487081809414157 35.0 33.0 35.0 25.0 35.5 98-99 31.41359575411436 35.0 33.0 35.0 25.0 35.0 100-101 31.34024639151711 35.0 33.0 35.0 24.5 35.0 102-103 30.251836881209954 34.0 30.5 35.0 21.5 35.0 104-105 31.067910570259755 35.0 32.5 35.0 23.5 35.0 106-107 31.16219845093287 35.0 33.0 35.0 24.0 35.0 108-109 31.139181648829435 35.0 33.0 35.0 23.0 35.0 110-111 31.067708598029405 35.0 33.0 35.0 23.0 35.0 112-113 30.991268607586214 35.0 33.0 35.0 21.5 35.0 114-115 30.893607951309356 35.0 33.0 35.0 19.5 35.0 116-117 30.79489333003869 35.0 33.0 35.0 19.0 35.0 118-119 30.661514111184907 35.0 33.0 35.0 17.5 35.0 120-121 30.52301548775052 35.0 33.0 35.0 6.0 35.0 122-123 30.357725386697233 35.0 33.0 35.0 2.0 35.0 124-125 29.539135880140364 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3795403918886642 1101 2 0.40091325466207905 1101 3 0.46242164259701823 1101 4 0.476176806857076 1101 5 0.47385257211237786 1101 6 0.42466323253864857 1101 7 0.39808646469049336 1101 8 0.3943313071607477 1101 9 0.4000256593646867 1101 10-11 0.4574358612991247 1101 12-13 0.47927176208867905 1101 14-15 0.6557361114673057 1101 16-17 0.5959174229141979 1101 18-19 0.6215855094677707 1101 20-21 0.6319932602064711 1101 22-23 0.6422206062668465 1101 24-25 0.6122772515442421 1101 26-27 0.6145142277052003 1101 28-29 0.6744931603961959 1101 30-31 0.6545744655457426 1101 32-33 0.6312796155157656 1101 34-35 0.6641545312248027 1101 36-37 0.6617461751714444 1101 38-39 0.6238908429592414 1101 40-41 0.7124567288250461 1101 42-43 0.6707054585540249 1101 44-45 0.6794599088186786 1101 46-47 0.654627626473065 1101 48-49 0.612116782288048 1101 50-51 0.6226466509806698 1101 52-53 0.630054894894819 1101 54-55 0.6336954701031701 1101 56-57 0.7016276964796333 1101 58-59 0.6564171982056095 1101 60-61 0.6316493420629143 1101 62-63 0.5854326827851821 1101 64-65 0.5986869480845982 1101 66-67 0.6332023132098143 1101 68-69 0.589102692165639 1101 70-71 0.5722870135346554 1101 72-73 0.5897430802011883 1101 74-75 0.6025557669545023 1101 76-77 0.5673801538811318 1101 78-79 0.508255175340274 1101 80-81 0.4547457391674641 1101 82-83 0.45232885522242583 1101 84-85 0.48291831381245487 1101 86-87 0.43429516683301017 1101 88-89 0.4130986262082672 1101 90-91 0.46294651624207006 1101 92-93 0.44478276184515053 1101 94-95 0.41734339014896804 1101 96-97 0.4710948752669246 1101 98-99 0.3966430526476543 1101 100-101 0.39493728177671983 1101 102-103 0.47275121900493744 1101 104-105 0.41157316019749146 1101 106-107 0.4244099318763226 1101 108-109 0.404839948607691 1101 110-111 0.3648799992737466 1101 112-113 0.3877205777054158 1101 114-115 0.3369281444582626 1101 116-117 0.33701784724630457 1101 118-119 0.36924804383115983 1101 120-121 0.42288323312939724 1101 122-123 0.5176045670295153 1101 124-125 0.5422981291411588 1102 1 0.4325552252851814 1102 2 0.39858927521427745 1102 3 0.37857774413198086 1102 4 0.449437895607403 1102 5 0.431991823747758 1102 6 0.4694475473712387 1102 7 0.4133753892490759 1102 8 0.4697065783804035 1102 9 0.5106957433318726 1102 10-11 0.5067622887816441 1102 12-13 0.5266086874861244 1102 14-15 0.5559757728561152 1102 16-17 0.5756199080525732 1102 18-19 0.5324066093492021 1102 20-21 0.5939285385242243 1102 22-23 0.5943094009626364 1102 24-25 0.6102454230599292 1102 26-27 0.6452336958323457 1102 28-29 0.6712729058328506 1102 30-31 0.6257943490725211 1102 32-33 0.5928760204941028 1102 34-35 0.5881192988833206 1102 36-37 0.6137025461112131 1102 38-39 0.7001461362797841 1102 40-41 0.6853405298526525 1102 42-43 0.6163472106532097 1102 44-45 0.6489158966228601 1102 46-47 0.6498961440206656 1102 48-49 0.6336092768636519 1102 50-51 0.6119096796069385 1102 52-53 0.6637716528418593 1102 54-55 0.6312924235703505 1102 56-57 0.6713126178763389 1102 58-59 0.6754018284192753 1102 60-61 0.7207230008867356 1102 62-63 0.7086469080043756 1102 64-65 0.6909631162448164 1102 66-67 0.7264650151625958 1102 68-69 0.6520233548692147 1102 70-71 0.7140027551641523 1102 72-73 0.7193291122877881 1102 74-75 0.7106749342193979 1102 76-77 0.7407872777831486 1102 78-79 0.7222376075656101 1102 80-81 0.6742612743070424 1102 82-83 0.6423722302032644 1102 84-85 0.6442155907267235 1102 86-87 0.6761079627548412 1102 88-89 0.7171848075160909 1102 90-91 0.7083074097949122 1102 92-93 0.650354400132894 1102 94-95 0.7010035246900479 1102 96-97 0.7681744747087578 1102 98-99 0.7548806693023522 1102 100-101 0.7811937245040035 1102 102-103 0.8306394966693453 1102 104-105 0.7939789491744769 1102 106-107 0.8509797593443231 1102 108-109 0.8852647854725966 1102 110-111 0.933282646538963 1102 112-113 0.9389594768560627 1102 114-115 0.9673897421739674 1102 116-117 0.9783981839216551 1102 118-119 0.939372038604386 1102 120-121 0.8628286867215991 1102 122-123 0.8834476669593414 1102 124-125 0.9573216012367887 1103 1 0.19838390523002047 1103 2 0.14338840708414935 1103 3 0.213089873085476 1103 4 0.18508209789980867 1103 5 0.26267818913107277 1103 6 0.23584085101331453 1103 7 0.31418304416735054 1103 8 0.24035518914612197 1103 9 0.26915379501274117 1103 10-11 0.20721867880718747 1103 12-13 0.23975385059964083 1103 14-15 0.2878069283282798 1103 16-17 0.2474446768903178 1103 18-19 0.33763375876254287 1103 20-21 0.2830791726661559 1103 22-23 0.3013072871561988 1103 24-25 0.30917269455405716 1103 26-27 0.33854666352615936 1103 28-29 0.3944182385030288 1103 30-31 0.3465641928543093 1103 32-33 0.1100750008933602 1103 34-35 0.3310849580065991 1103 36-37 0.3318388922780997 1103 38-39 0.39688923532348497 1103 40-41 0.430850104152384 1103 42-43 0.452342112649454 1103 44-45 0.3642387325146146 1103 46-47 0.37961999273090896 1103 48-49 0.3857677332197653 1103 50-51 0.3362407785602102 1103 52-53 0.39262539326190904 1103 54-55 0.3985151419028128 1103 56-57 0.4322436535284595 1103 58-59 0.44423081356610794 1103 60-61 0.4784587411711385 1103 62-63 0.4692690679302274 1103 64-65 0.36204437910286913 1103 66-67 0.44337207550216107 1103 68-69 0.4842182093895957 1103 70-71 0.4553731686563438 1103 72-73 0.4471366880796168 1103 74-75 0.4236528014226195 1103 76-77 0.4666802762232294 1103 78-79 0.46111808141996846 1103 80-81 0.4425318528343851 1103 82-83 0.36121817101668086 1103 84-85 0.348757166021187 1103 86-87 0.3755320352798748 1103 88-89 0.4035227791662237 1103 90-91 0.3818512006298107 1103 92-93 0.40771937650800183 1103 94-95 0.3916154546970567 1103 96-97 0.3995495227498118 1103 98-99 0.4211281542118108 1103 100-101 0.3801147789203867 1103 102-103 0.43417362277429206 1103 104-105 0.4112813591398883 1103 106-107 0.43045464235022024 1103 108-109 0.4211000216085097 1103 110-111 0.43173459417986493 1103 112-113 0.3940614369311213 1103 114-115 0.4312473258784415 1103 116-117 0.40849788324116787 1103 118-119 0.4482780024775117 1103 120-121 0.4237676350181161 1103 122-123 0.3943693769948311 1103 124-125 0.4802394453803025 1104 1 0.28322764687235846 1104 2 0.2911054509021227 1104 3 0.3308209648127338 1104 4 0.31066570146001027 1104 5 0.2909234150337596 1104 6 0.23423839196560436 1104 7 0.260449047141023 1104 8 0.2719773828319276 1104 9 0.25251930890360796 1104 10-11 0.2705169372766534 1104 12-13 0.29371559829950655 1104 14-15 0.3413857267232814 1104 16-17 0.43501265923974586 1104 18-19 0.48267787917158245 1104 20-21 0.4132798651279401 1104 22-23 0.3443192465801488 1104 24-25 0.40072538869210206 1104 26-27 0.4640703432820885 1104 28-29 0.46608724916906397 1104 30-31 0.39427902471978626 1104 32-33 -0.21901683977689146 1104 34-35 0.3797836261296723 1104 36-37 0.41187199917452233 1104 38-39 0.5293493166187702 1104 40-41 0.5043567044175745 1104 42-43 0.5382226768662548 1104 44-45 0.4536625430170531 1104 46-47 0.4723195038089685 1104 48-49 0.4129656197192517 1104 50-51 0.35187875995648454 1104 52-53 0.3890270894675183 1104 54-55 0.4494537940033041 1104 56-57 0.4568738854496672 1104 58-59 0.39844627221848583 1104 60-61 0.3963107060485527 1104 62-63 0.4697752229897034 1104 64-65 0.538230417982561 1104 66-67 0.47970544650571867 1104 68-69 0.5042026171352916 1104 70-71 0.5427539594977375 1104 72-73 0.4162913723314432 1104 74-75 0.45315087722502057 1104 76-77 0.4755310623310436 1104 78-79 0.47148115934786006 1104 80-81 0.46004993353525947 1104 82-83 0.47651285738393767 1104 84-85 0.4402185467380306 1104 86-87 0.4426892258749717 1104 88-89 0.4883164904645909 1104 90-91 0.42763666697575786 1104 92-93 0.42100665564956685 1104 94-95 0.406202054849647 1104 96-97 0.4489981835332202 1104 98-99 0.454514460456231 1104 100-101 0.4759966260696409 1104 102-103 0.42225984548734274 1104 104-105 0.45249288976678415 1104 106-107 0.4675767554002981 1104 108-109 0.4143841517340583 1104 110-111 0.43969569277221865 1104 112-113 0.4167474028163767 1104 114-115 0.45733760732325734 1104 116-117 0.4405092800248269 1104 118-119 0.468076385017401 1104 120-121 0.45817912092335433 1104 122-123 0.49855236016268023 1104 124-125 0.6086217922297372 1105 1 0.211120319839452 1105 2 0.21982058947459393 1105 3 0.2353345297007543 1105 4 0.2278079117708245 1105 5 0.22675838486822641 1105 6 0.23394758332106136 1105 7 0.19750473112446087 1105 8 0.21013731022240023 1105 9 0.23542748914574219 1105 10-11 0.36057529318806303 1105 12-13 0.341072485698966 1105 14-15 0.46458479545346165 1105 16-17 0.4093181476938099 1105 18-19 0.391518283674273 1105 20-21 0.4237422904069277 1105 22-23 0.4091678107788326 1105 24-25 0.4171908051567854 1105 26-27 0.4655206462629877 1105 28-29 0.46632833003925356 1105 30-31 0.4319299846576783 1105 32-33 0.030791816817014706 1105 34-35 0.4432218866172377 1105 36-37 0.44097183615036784 1105 38-39 0.4218948829380764 1105 40-41 0.5481540831553531 1105 42-43 0.5222618248337483 1105 44-45 0.43831826887569747 1105 46-47 0.3598207629914114 1105 48-49 0.37737832365787227 1105 50-51 0.38249027476177844 1105 52-53 0.4339905709481542 1105 54-55 0.4433334018600803 1105 56-57 0.4916661766446637 1105 58-59 0.48847352404656164 1105 60-61 0.4633160525788469 1105 62-63 0.527962687861951 1105 64-65 0.5183561102615855 1105 66-67 0.4999094381305156 1105 68-69 0.4428630198852943 1105 70-71 0.45358425015405857 1105 72-73 0.45118135554143635 1105 74-75 0.37396893128302366 1105 76-77 0.41818749678139966 1105 78-79 0.41777182977786964 1105 80-81 0.4617245541264907 1105 82-83 0.4526103480072692 1105 84-85 0.379452136698454 1105 86-87 0.38004281139672713 1105 88-89 0.3856693721232247 1105 90-91 0.3280796085018949 1105 92-93 0.41958650715692514 1105 94-95 0.4145683943633678 1105 96-97 0.35599435813794145 1105 98-99 0.3494158364814979 1105 100-101 0.33686269320461903 1105 102-103 0.3869059172586695 1105 104-105 0.4887864907634487 1105 106-107 0.486124788883874 1105 108-109 0.43210826615041853 1105 110-111 0.4315794262125898 1105 112-113 0.4392404224205535 1105 114-115 0.46915725588703694 1105 116-117 0.5202619132228818 1105 118-119 0.5364670163357239 1105 120-121 0.5089419151374592 1105 122-123 0.49761848186255264 1105 124-125 0.5481760352511138 1106 1 0.1977851356385294 1106 2 0.23568759309871723 1106 3 0.22562177394219063 1106 4 0.19670179664459653 1106 5 0.1238185826395366 1106 6 0.16195177560403096 1106 7 0.21111641412650783 1106 8 0.22772835853977114 1106 9 0.35138072328796 1106 10-11 0.3473137024691866 1106 12-13 0.28889725243421793 1106 14-15 0.37858880355473445 1106 16-17 0.3255904867944679 1106 18-19 0.4070430578084654 1106 20-21 0.39244458333319443 1106 22-23 0.2971465389250838 1106 24-25 0.3491792840717167 1106 26-27 0.37345310724486325 1106 28-29 0.43228718692714097 1106 30-31 0.41748563750823564 1106 32-33 0.37021847551001485 1106 34-35 0.40648883987423545 1106 36-37 0.3413551105523567 1106 38-39 0.41905087102645666 1106 40-41 0.47197374212960597 1106 42-43 0.3952637419976952 1106 44-45 0.4010633363022649 1106 46-47 0.37833769763791025 1106 48-49 0.3653430458501177 1106 50-51 0.32426119980412693 1106 52-53 0.2897830963845678 1106 54-55 0.3729533698703875 1106 56-57 0.39789614490612735 1106 58-59 0.3496718612524674 1106 60-61 0.3883263873437812 1106 62-63 0.31143963313397194 1106 64-65 0.3551811460288121 1106 66-67 0.3407557552547331 1106 68-69 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2216 72-73 -1.804100063476028 2216 74-75 -1.8143801993263011 2216 76-77 -1.9312810768005733 2216 78-79 -1.946735903553332 2216 80-81 -1.7925586214003886 2216 82-83 -1.5120386943608288 2216 84-85 -1.498379340841602 2216 86-87 -1.53937162337877 2216 88-89 -1.560361901128239 2216 90-91 -1.579681977857927 2216 92-93 -1.6911418871287118 2216 94-95 -1.6887628701404722 2216 96-97 -1.5989310692067278 2216 98-99 -1.6583575076150687 2216 100-101 -1.6814434210513127 2216 102-103 -1.8347201860477682 2216 104-105 -1.7045515456645823 2216 106-107 -1.7109619532784386 2216 108-109 -1.735288394652347 2216 110-111 -1.6842991727441472 2216 112-113 -1.72143997635132 2216 114-115 -1.7267155456905954 2216 116-117 -1.9380658904817878 2216 118-119 -2.043563440692658 2216 120-121 -2.02731001441191 2216 122-123 -2.0725513583952093 2216 124-125 -2.3318333343512627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59720.0 3 10323.0 4 5822.0 5 4704.0 6 5338.0 7 6033.0 8 7013.0 9 8050.0 10 8916.0 11 9893.0 12 10710.0 13 10857.0 14 10948.0 15 9791.0 16 8684.0 17 8324.0 18 7967.0 19 7785.0 20 8185.0 21 8901.0 22 10871.0 23 13865.0 24 18711.0 25 24279.0 26 30140.0 27 35752.0 28 43322.0 29 54026.0 30 64360.0 31 78686.0 32 101165.0 33 134266.0 34 184237.0 35 273045.0 36 508987.0 37 1065199.0 38 561574.0 39 3289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17997639890488 18.652788917522066 12.570914071411948 24.596320612161108 2 15.710583198981606 19.788732766346552 40.186200634910946 24.314483399760896 3 18.87507717371657 24.31943345688953 29.43737132823302 27.368118041160884 4 12.319853755653984 15.88095052956357 37.95548408961867 33.84371162516377 5 13.372045593135088 38.0401360915525 35.129086840237974 13.458731475074433 6 32.698628487871915 36.468510422141506 17.486618140604218 13.346242949382365 7 28.652644565665142 32.00734370765631 21.501484625690235 17.838527100988312 8 25.75456509348946 36.05900449586868 20.06820588970249 18.118224520939368 9 26.268243936502788 15.657204574646338 20.020273813949583 38.054277674901286 10-11 24.767081041787517 26.29658783014781 28.589489606355873 20.346841521708804 12-13 25.05591234495229 23.784806058918424 28.377167319344526 22.78211427678476 14-15 22.315645482173217 25.643172451079188 25.604084845653148 26.437097221094447 16-17 22.48990612131213 28.70731547241584 26.362279029619234 22.440499376652795 18-19 22.370199381503248 28.054760163640264 27.51996311339401 22.055077341462486 20-21 23.176625636469765 27.33395251852967 27.36586135880558 22.123560486194986 22-23 22.80357644532187 27.323785552501413 27.092940554659485 22.77969744751724 24-25 22.609184847804453 27.431998694814308 27.33582789053181 22.62298856684943 26-27 22.562391911198 27.702146620110156 27.16070396356937 22.57475750512247 28-29 22.433709016378398 27.706226910131637 27.271038732614095 22.58902534087587 30-31 22.687823310182335 27.491502840511544 27.17881089545311 22.64186295385301 32-33 22.486501646821115 27.490253432774015 27.27159259275575 22.751652327649122 34-35 22.824797815168917 27.608910067488186 27.098134291813096 22.468157825529804 36-37 22.84400138127851 27.420242246194842 27.04649575424161 22.689260618285033 38-39 22.533941922495842 27.61310477594414 27.18896828468405 22.66398501687597 40-41 22.8115502589956 27.536965629021594 27.121800992087824 22.52968311989498 42-43 22.639761190175577 27.49662715603411 27.052695597439058 22.810916056351253 44-45 22.629589169336597 27.566512278992445 27.16129415657449 22.642604395096473 46-47 22.65740475932986 27.67013648248748 26.927084563792665 22.74537419439 48-49 22.6336240056661 27.494482567731822 27.166239742595167 22.705653684006915 50-51 22.672503730702694 27.61386163311602 26.994014734991715 22.71961990118957 52-53 22.74138840776885 27.546853201031357 27.020611241246662 22.691147149953135 54-55 22.68208041261647 27.361193904593446 27.14359722768541 22.813128455104675 56-57 22.56953521142876 27.641704315711557 27.147873243374633 22.64088722948505 58-59 22.62381425096695 27.44895854210273 27.159833408991606 22.767393797938713 60-61 22.74605875533341 27.49590155155653 27.09174920048345 22.666290492626608 62-63 22.44987409185975 27.517850987741692 27.241734971765496 22.79053994863306 64-65 22.434812619526152 27.373747437795043 27.23810409819263 22.953335844486173 66-67 22.470232723180835 27.51529783712694 27.05377260556454 22.960696834127685 68-69 22.41895360663605 27.402479420108634 27.233672209918563 22.944894763336755 70-71 22.463627420808916 27.311298969634983 27.23194755263177 22.993126056924332 72-73 22.494212660579223 27.346330947162752 27.098105016946644 23.061351375311386 74-75 22.371446010501728 27.564281214048812 27.02989039947155 23.034382375977906 76-77 22.5232408278968 27.45935404983148 27.081988692247204 22.93541643002451 78-79 22.58220663300876 27.320629866064078 27.178935540089633 22.91822796083753 80-81 22.465087165593715 27.446011780582076 27.1190408225714 22.969860231252813 82-83 22.565239578332623 27.44336818462206 27.108142415401392 22.883249821643922 84-85 22.65968029159501 27.330662883774394 27.009184550137267 23.000472274493333 86-87 22.48721446894647 27.2314238718129 27.22280993622606 23.058551723014574 88-89 22.603085899780165 27.234004772373066 27.20394997519892 22.958959352647845 90-91 22.595005820800345 27.348414884697682 27.152104101206508 22.90447519329547 92-93 22.530915779692158 27.14302672155762 27.26987536258976 23.056182136160462 94-95 22.477964815011262 27.18057473343783 27.243372057860988 23.098088393689924 96-97 22.45879666999104 27.221896044169302 27.18840288848026 23.130904397359394 98-99 22.497054478292892 27.022466917325648 27.159983258115105 23.320495346266352 100-101 22.412637350470256 27.092910488856 27.223692876378085 23.270759284295657 102-103 22.466325300674814 26.961226806028453 27.28964493831071 23.282802954986025 104-105 22.370335676306862 27.022095941913925 27.238552030333473 23.369016351445737 106-107 22.347307454469494 27.06479227983416 27.204539845579674 23.38336042011667 108-109 22.427286158492286 27.066229938265717 27.084107566123024 23.422376337118973 110-111 22.333732868599153 27.27578509706565 27.027761271053162 23.36272076328203 112-113 22.4478117604536 27.234362524480716 27.15454853903441 23.163277176031276 114-115 22.2927251207879 27.368860477161157 26.969844939420135 23.368569462630813 116-117 22.28103588246036 27.264450997403177 27.10811379658563 23.34639932355083 118-119 22.386241279155932 27.31761759438086 26.980962859248297 23.315178267214907 120-121 22.391836860457353 27.433348763813154 26.943847970402825 23.23096640532667 122-123 22.314339295976147 27.552701758050212 26.923431194442156 23.209527751531482 124-125 22.411984555042135 27.555091568568574 26.778912466201504 23.254011410187786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14233.0 1 7492.0 2 770.0 3 728.0 4 591.5 5 465.5 6 404.5 7 385.5 8 369.5 9 370.5 10 361.0 11 346.5 12 369.5 13 405.0 14 454.0 15 504.5 16 597.5 17 737.0 18 894.0 19 1163.5 20 1597.5 21 2406.5 22 3539.0 23 5148.0 24 7745.0 25 11319.5 26 16134.0 27 21385.5 28 26287.0 29 32210.5 30 39207.0 31 46055.0 32 53340.5 33 60896.0 34 68487.5 35 75762.5 36 84552.0 37 93444.0 38 98823.0 39 102723.0 40 106795.0 41 112685.5 42 117665.0 43 122128.0 44 126492.5 45 129557.5 46 132308.0 47 134253.5 48 134783.0 49 133271.0 50 130291.5 51 124315.5 52 119139.0 53 117197.0 54 114651.5 55 106590.5 56 95227.5 57 85915.0 58 77412.5 59 67755.5 60 57350.0 61 47071.5 62 38159.5 63 29869.5 64 22322.5 65 16885.5 66 12480.5 67 9835.0 68 7403.5 69 4759.0 70 3437.0 71 2619.5 72 1996.5 73 1617.5 74 1290.0 75 1015.5 76 668.0 77 331.0 78 177.0 79 119.0 80 74.0 81 44.0 82 35.0 83 25.0 84 16.5 85 12.0 86 12.0 87 12.0 88 8.0 89 9.5 90 11.5 91 11.0 92 11.5 93 9.0 94 4.5 95 2.5 96 4.0 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47518238837200744 2 0.46779280423910946 3 0.4627106396575906 4 0.45981906325776095 5 0.4682601297178698 6 0.5259748263447729 7 0.5207466225511415 8 0.5306188732899538 9 0.5362559868775005 10-11 0.5553287079794073 12-13 0.5637697744395161 14-15 0.5422874063377513 16-17 0.5354527712108812 18-19 0.5464203160405382 20-21 0.5469314532829322 22-23 0.5570665746035474 24-25 0.5508160963251277 26-27 0.5587460255428424 28-29 0.5382859319258658 30-31 0.5441275004103702 32-33 0.5459821984042004 34-35 0.5439522533558351 36-37 0.4892167566560292 38-39 0.45672303196097364 40-41 0.4548245222035097 42-43 0.46748612189367295 44-45 0.4728895727418395 46-47 0.4762192667780069 48-49 0.4909546232801692 50-51 0.47325467077212097 52-53 0.472582890396403 54-55 0.462287125942464 56-57 0.446441871428246 58-59 0.46355766708784374 60-61 0.4597606475729159 62-63 0.47589798051135923 64-65 0.4752846158204862 66-67 0.4835942469896938 68-69 0.49340808204366104 70-71 0.48898309391664896 72-73 0.5013234073401645 74-75 0.4899469527165922 76-77 0.48762492924400175 78-79 0.4997169760069258 80-81 0.4924588271649291 82-83 0.49447416829208307 84-85 0.49130511738923954 86-87 0.48100935293530056 88-89 0.4856680038016928 90-91 0.4711517061176994 92-93 0.4712247257237557 94-95 0.46585048271801166 96-97 0.4550289771004674 98-99 0.41840234270262505 100-101 0.41643081333910487 102-103 0.4062226724124335 104-105 0.4100635036909951 106-107 0.4094501390001221 108-109 0.42202411516301774 110-111 0.4437985616890077 112-113 0.44359410679205014 114-115 0.4373436285136304 116-117 0.43112235807763327 118-119 0.4743645687841768 120-121 0.4667559258331099 122-123 0.4320131972715202 124-125 0.44154955782247357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3423738.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.85367447679909 #Duplication Level Percentage of deduplicated Percentage of total 1 81.39565383080364 51.97411583538361 2 10.545152224723534 13.466934349315803 3 3.48907847050689 6.683714426392651 4 1.5820884634563739 4.040886469561702 5 0.8683958727708682 2.7725133688453427 6 0.5234535926222359 2.0054661184206752 7 0.3386879705067387 1.5138529992561516 8 0.22699861893203127 1.159575673597511 9 0.1603643110364876 0.9215865463158035 >10 0.7894570215931035 9.12359410046653 >50 0.054193285656899584 2.3918151576745483 >100 0.026230048304304067 2.7840738547667776 >500 1.231445434615834E-4 0.042854612175255415 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.231445434615834E-4 1.1190164878276325 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 14231 0.415656805514908 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.9207842422521816E-5 0.0 0.0 0.0 0.0 3 2.9207842422521816E-5 0.0 0.0 0.0 0.0 4 2.9207842422521816E-5 0.0 0.0 0.0 0.0 5 2.9207842422521816E-5 0.0 0.0 0.0 2.9207842422521816E-5 6 2.9207842422521816E-5 0.0 0.0 0.0 2.9207842422521816E-5 7 5.841568484504363E-5 0.0 0.0 0.0 2.9207842422521816E-5 8 5.841568484504363E-5 0.0 0.0 0.0 2.9207842422521816E-5 9 5.841568484504363E-5 0.0 0.0 0.0 2.9207842422521816E-5 10-11 5.841568484504363E-5 1.4603921211260908E-5 0.0 0.0 2.9207842422521816E-5 12-13 1.0222744847882635E-4 2.9207842422521816E-5 0.0 0.0 2.9207842422521816E-5 14-15 1.898509757463918E-4 5.841568484504363E-5 0.0 0.0 2.9207842422521816E-5 16-17 2.482666605914354E-4 8.762352726756545E-5 0.0 0.0 2.9207842422521816E-5 18-19 3.5049410907026185E-4 8.762352726756545E-5 0.0 5.841568484504363E-5 2.9207842422521816E-5 20-21 4.0890979391530546E-4 8.762352726756545E-5 0.0 8.762352726756545E-5 2.9207842422521816E-5 22-23 4.3811763633782723E-4 8.762352726756545E-5 0.0 1.460392121126091E-4 2.9207842422521816E-5 24-25 6.425725332954799E-4 8.762352726756545E-5 0.0 2.6287058180269636E-4 2.9207842422521816E-5 26-27 6.425725332954799E-4 8.762352726756545E-5 0.0 5.695529272391754E-4 2.9207842422521816E-5 28-29 8.470274302531326E-4 8.762352726756545E-5 0.0 0.0018546979938301354 2.9207842422521816E-5 30-31 9.638587999432199E-4 8.762352726756545E-5 0.0 0.005651717508757972 2.9207842422521816E-5 32-33 9.638587999432199E-4 1.0222744847882635E-4 0.0 0.015377929035457736 2.9207842422521816E-5 34-35 0.0010368784059995246 1.1683136969008727E-4 0.0 0.029762791428549727 2.9207842422521816E-5 36-37 0.0011391058544783508 1.1683136969008727E-4 0.0 0.05514440649372119 2.9207842422521816E-5 38-39 0.0012267293817459163 2.3366273938017453E-4 0.0 0.09491088395198463 2.9207842422521816E-5 40-41 0.0012413333029571772 2.920784242252182E-4 0.0 0.13914616130089394 2.9207842422521816E-5 42-43 0.0013289568302247427 2.920784242252182E-4 0.0 0.18336683472859197 2.9207842422521816E-5 44-45 0.001431184278703569 2.920784242252182E-4 0.0 0.2398840098161717 2.9207842422521816E-5 46-47 0.0016064313332386998 2.920784242252182E-4 0.0 0.3123632707876596 2.9207842422521816E-5 48-49 0.0016940548605062654 2.920784242252182E-4 0.0 0.39145810806784864 2.9207842422521816E-5 50-51 0.0016940548605062654 2.920784242252182E-4 0.0 0.47610243540831687 2.9207842422521816E-5 52-53 0.0016940548605062654 3.2128626664773997E-4 0.0 0.5606007235366726 2.9207842422521816E-5 54-55 0.0016940548605062654 3.2128626664773997E-4 0.0 0.6557014584644034 2.9207842422521816E-5 56-57 0.0018693019150413962 3.3589018785900085E-4 0.0 0.7745919810452786 2.9207842422521816E-5 58-59 0.001898509757463918 3.504941090702618E-4 0.0 0.910291616940315 2.9207842422521816E-5 60-61 0.001898509757463918 3.504941090702618E-4 0.0 1.0577912211740501 2.9207842422521816E-5 62-63 0.0019277175998864398 3.504941090702618E-4 0.0 1.2078611155409673 2.9207842422521816E-5 64-65 0.0019277175998864398 3.504941090702618E-4 0.0 1.363188421543938 2.9207842422521816E-5 66-67 0.0020445489695765273 3.504941090702618E-4 0.0 1.5468911464603892 2.9207842422521816E-5 68-69 0.002102964654421571 3.504941090702618E-4 0.0 1.7600645843811646 2.9207842422521816E-5 70-71 0.002219796024111658 4.3811763633782723E-4 0.0 1.9966627119248026 2.9207842422521816E-5 72-73 0.0023220234725904844 4.3811763633782723E-4 0.0 2.2369410276136783 2.9207842422521816E-5 74-75 0.0023366273938017455 4.3811763633782723E-4 0.0 2.483995562744579 2.9207842422521816E-5 76-77 0.0023366273938017455 4.3811763633782723E-4 0.0 2.7576292344799747 2.9207842422521816E-5 78-79 0.0023512313150130066 4.3811763633782723E-4 0.0 3.0786234227034894 2.9207842422521816E-5 80-81 0.002541082290759398 4.3811763633782723E-4 0.0 3.436083602191523 2.9207842422521816E-5 82-83 0.0025994979756044415 4.8192939997160995E-4 0.0 3.80366429907896 2.9207842422521816E-5 84-85 0.0026287058180269633 5.111372423941317E-4 0.0 4.170923709699749 2.9207842422521816E-5 86-87 0.0026871215028720073 5.549490060279145E-4 0.0 4.559271182549599 2.9207842422521816E-5 88-89 0.002716329345294529 5.549490060279145E-4 0.0 4.994175956220949 2.9207842422521816E-5 90-91 0.002862368557407138 5.841568484504364E-4 0.0 5.476178375798615 2.9207842422521816E-5 92-93 0.0029207842422521815 5.841568484504364E-4 0.0 5.979648559556836 2.9207842422521816E-5 94-95 0.0029207842422521815 5.841568484504364E-4 0.0 6.48618556676942 2.9207842422521816E-5 96-97 0.0029207842422521815 5.841568484504364E-4 0.0 6.993423562200145 2.9207842422521816E-5 98-99 0.003154446981632356 5.841568484504364E-4 0.0 7.549803752506763 2.9207842422521816E-5 100-101 0.0034173175634350525 5.841568484504364E-4 0.0 8.166279078597722 2.9207842422521816E-5 102-103 0.0037093959876602707 5.841568484504364E-4 0.0 8.81361249020807 2.9207842422521816E-5 104-105 0.004235137151265663 5.987607696616973E-4 0.0 9.457616207782255 2.9207842422521816E-5 106-107 0.0043957802845895335 6.133646908729582E-4 0.0 10.094215737302328 2.9207842422521816E-5 108-109 0.004424988127012055 6.133646908729582E-4 0.0 10.761074591572136 2.9207842422521816E-5 110-111 0.0044980077330683595 6.133646908729582E-4 0.0 11.48258131901448 2.9207842422521816E-5 112-113 0.004556423417913403 6.133646908729582E-4 0.0 12.261554476423138 2.9207842422521816E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4955 0.0 46.958893 1 GGTATCA 3320 0.0 45.707436 1 TCAACGC 5310 0.0 41.930264 4 ATCAACG 5310 0.0 41.922256 3 CAACGCA 5570 0.0 39.976536 5 AACGCAG 5960 0.0 37.46107 6 GTACATG 12265 0.0 37.068974 1 TACATGG 12280 0.0 36.249924 2 TATCAAC 6420 0.0 35.41051 2 ACATGGG 12430 0.0 34.046 3 ACGCAGA 7260 0.0 30.098421 7 CGCAGAG 7280 0.0 29.36144 8 CATGGGA 7405 0.0 27.25368 4 CATGGGG 6105 0.0 26.036112 4 GAGTACA 8500 0.0 24.573837 1 AGTACAT 8490 0.0 22.991644 2 GCAGAGT 9215 0.0 21.710238 9 ATGGGAT 3395 0.0 20.167257 5 AGAGTAC 7780 0.0 19.859818 10-11 CAGAGTA 8205 0.0 19.701927 10-11 >>END_MODULE