FastQCFastQC Report
Thu 26 May 2016
SRR1364085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423738
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62330.18205248181957848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60730.177379227031975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54150.066.912161
GTATCAA86750.049.59841
ATCAACG110250.038.7989733
TCAACGC110850.038.5369834
CAACGCA112900.037.942645
AACGCAG117050.036.6482166
TATCAAC118850.035.9925272
GTACATG113950.031.1696471
ACGCAGA140050.030.3769137
TACATGG114400.030.319722
CGCAGAG140500.030.237278
ACATGGG115700.028.9497623
GTGGTAT27900.027.9812471
TGGTATC25100.027.7328662
GCAGAGT164100.025.779939
GAGTACT106400.025.33208312-13
CAGAGTA153750.024.74816110-11
AGAGTAC148100.024.32633210-11
CATGGGA68050.024.218134
GAGTACA85300.023.7536221
Produced by FastQC (version 0.11.5)