FastQCFastQC Report
Thu 26 May 2016
SRR1364084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471515
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101940.41245956427535335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.053.7451671
GTATCAA38000.042.1288951
TCAACGC40300.038.107074
ATCAACG41150.037.3168873
CAACGCA42650.036.0073855
GTACATG78150.033.963881
AACGCAG45400.033.958816
TATCAAC47250.033.3783072
TACATGG78000.032.8853842
ACATGGG78100.032.0838623
ACGCAGA52450.029.0543967
CGCAGAG53950.027.6948918
CATGGGG52900.027.5677494
ATGGGGG28100.021.182865
CGTACAC3108.42374E-921.1196423
GTATAGC14700.020.6473461
ATAGGCG1450.003859647520.525465
GCAGAGT70700.020.1231449
GTGTAGC21350.020.0699391
GAGTACA58050.019.9914281
Produced by FastQC (version 0.11.5)