##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2471515 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.029435791407295 31.0 31.0 34.0 28.0 34.0 2 31.05308808564787 31.0 31.0 34.0 28.0 34.0 3 31.169024262446314 33.0 31.0 34.0 28.0 34.0 4 34.65119289180927 37.0 35.0 37.0 32.0 37.0 5 34.71410369752965 37.0 35.0 37.0 32.0 37.0 6 34.745473120737685 37.0 35.0 37.0 32.0 37.0 7 34.669584849778374 37.0 35.0 37.0 32.0 37.0 8 34.64768492200128 37.0 35.0 37.0 32.0 37.0 9 36.177252414005174 39.0 37.0 39.0 32.0 39.0 10-11 36.09333708272052 39.0 37.0 39.0 31.0 39.0 12-13 35.996549484830155 39.0 37.0 39.0 31.0 39.0 14-15 37.262241580569004 40.0 38.0 41.0 31.0 41.0 16-17 37.260948244295506 40.0 38.0 41.0 31.0 41.0 18-19 37.25710768496246 40.0 37.5 41.0 31.0 41.0 20-21 37.2752999678335 40.0 38.0 41.0 31.0 41.0 22-23 37.22798445487889 40.0 37.0 41.0 31.0 41.0 24-25 37.23162978982527 40.0 37.0 41.0 31.0 41.0 26-27 37.19351227890586 40.0 37.0 41.0 31.0 41.0 28-29 37.15032298003452 40.0 37.0 41.0 31.0 41.0 30-31 37.10092332031164 40.0 37.0 41.0 31.0 41.0 32-33 37.00730118975608 40.0 37.0 41.0 30.0 41.0 34-35 36.98475570652009 40.0 37.0 41.0 30.0 41.0 36-37 36.91977653382642 40.0 37.0 41.0 30.0 41.0 38-39 36.83765220927245 40.0 37.0 41.0 30.0 41.0 40-41 36.762371865030154 40.0 37.0 41.0 30.0 41.0 42-43 36.653201578788725 40.0 36.0 41.0 30.0 41.0 44-45 36.551426756463144 40.0 36.0 41.0 30.0 41.0 46-47 36.453698440025654 40.0 36.0 41.0 30.0 41.0 48-49 36.344230765340285 40.0 36.0 41.0 29.5 41.0 50-51 36.214602581817225 40.0 35.0 41.0 29.0 41.0 52-53 36.07857427529268 39.5 35.0 41.0 29.0 41.0 54-55 35.90699247222857 39.0 35.0 41.0 28.0 41.0 56-57 35.72936377080455 39.0 35.0 41.0 28.0 41.0 58-59 35.528042921042356 39.0 35.0 41.0 28.0 41.0 60-61 35.292489019892656 38.5 34.5 40.5 28.0 41.0 62-63 35.03372384954167 38.0 34.0 40.0 27.5 41.0 64-65 34.759332433750146 37.5 34.0 40.0 27.0 41.0 66-67 34.458763349605405 37.0 34.0 40.0 26.5 41.0 68-69 34.13226138623476 36.5 34.0 39.5 26.0 41.0 70-71 33.78678280326035 36.0 33.5 39.0 26.0 41.0 72-73 33.4330058688699 35.5 33.0 39.0 26.0 40.0 74-75 33.161788012615744 35.0 33.0 37.5 26.0 40.0 76-77 32.93582235996949 35.0 33.0 37.0 26.0 39.0 78-79 32.632351816598316 35.0 33.0 37.0 26.0 39.0 80-81 32.41920158283482 35.0 33.0 36.0 25.5 39.0 82-83 32.31111605634601 35.0 33.0 36.0 26.0 37.0 84-85 32.098196450355346 35.0 33.0 36.0 26.0 37.0 86-87 31.902487745370756 35.0 33.0 35.0 25.5 37.0 88-89 31.71412069115502 35.0 33.0 35.0 25.0 36.0 90-91 31.569066341899607 35.0 33.0 35.0 25.0 36.0 92-93 31.447196557577033 35.0 33.0 35.0 25.0 36.0 94-95 31.34270619437875 35.0 33.0 35.0 24.0 36.0 96-97 31.256105870286042 35.0 33.0 35.0 24.0 35.0 98-99 31.174428639923285 35.0 33.0 35.0 23.5 35.0 100-101 31.095973926923364 35.0 33.0 35.0 23.0 35.0 102-103 29.987953340360065 34.0 30.5 35.0 19.5 35.0 104-105 30.816330267062916 35.0 32.0 35.0 20.0 35.0 106-107 30.909612120500988 35.0 33.0 35.0 20.0 35.0 108-109 30.882075973643694 35.0 33.0 35.0 20.0 35.0 110-111 30.801158196490817 35.0 33.0 35.0 19.0 35.0 112-113 30.716800424031415 35.0 33.0 35.0 18.5 35.0 114-115 30.605292705081702 35.0 32.5 35.0 17.5 35.0 116-117 30.49672306257498 35.0 32.0 35.0 10.0 35.0 118-119 30.351104282191287 35.0 32.0 35.0 2.0 35.0 120-121 30.198580829976756 35.0 32.0 35.0 2.0 35.0 122-123 30.026103422394765 35.0 32.0 35.0 2.0 35.0 124-125 29.197241368148685 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3704269533714104 1101 2 0.4002448227860569 1101 3 0.35077883897570317 1101 4 0.4320935823363996 1101 5 0.36313067904594476 1101 6 0.3378146584565158 1101 7 0.40576308205704237 1101 8 0.3206605170327279 1101 9 0.37502064547146574 1101 10-11 0.44820889024222765 1101 12-13 0.5700047358141731 1101 14-15 0.6368374656524054 1101 16-17 0.5114386449390054 1101 18-19 0.5339247159514571 1101 20-21 0.5404230811785382 1101 22-23 0.5994115957759121 1101 24-25 0.5307131625663004 1101 26-27 0.5078130868193682 1101 28-29 0.634126473746754 1101 30-31 0.5612479992109698 1101 32-33 0.6020293212802414 1101 34-35 0.5777572406016134 1101 36-37 0.5459705593301578 1101 38-39 0.5657611006373813 1101 40-41 0.6377974308554712 1101 42-43 0.6285509341445135 1101 44-45 0.5489284615646426 1101 46-47 0.5596028833369502 1101 48-49 0.5308104573853711 1101 50-51 0.5918704131236083 1101 52-53 0.5657859384030814 1101 54-55 0.6226270656423054 1101 56-57 0.6464296055961185 1101 58-59 0.6894687570297009 1101 60-61 0.5875676173353384 1101 62-63 0.6297237571448946 1101 64-65 0.6703632438352898 1101 66-67 0.6494489203863907 1101 68-69 0.5959626964799156 1101 70-71 0.6892076611810509 1101 72-73 0.6522959016561529 1101 74-75 0.7219747301924073 1101 76-77 0.6644547631679458 1101 78-79 0.5923702549560446 1101 80-81 0.5632145198578868 1101 82-83 0.547871063622587 1101 84-85 0.5912079040834257 1101 86-87 0.5449253200371764 1101 88-89 0.567263936156376 1101 90-91 0.640900931805735 1101 92-93 0.6105955066367521 1101 94-95 0.6074701564593425 1101 96-97 0.6039656415385402 1101 98-99 0.5812249840514667 1101 100-101 0.5705868719548057 1101 102-103 0.6865059872157389 1101 104-105 0.5732472988858 1101 106-107 0.519991940910284 1101 108-109 0.6052887841270085 1101 110-111 0.5765394258786003 1101 112-113 0.5409369650604212 1101 114-115 0.5856957012342647 1101 116-117 0.5985481817396447 1101 118-119 0.5366277814925837 1101 120-121 0.6371039397109008 1101 122-123 0.7107400350017521 1101 124-125 0.757028617657113 1102 1 0.34303648073215953 1102 2 0.39861220673405384 1102 3 0.3835713896182753 1102 4 0.363299428113379 1102 5 0.4173539806067623 1102 6 0.3649860905646136 1102 7 0.4317725077858938 1102 8 0.3970315088184151 1102 9 0.4791222412727052 1102 10-11 0.3708518885970946 1102 12-13 0.3982804523914467 1102 14-15 0.483408306089089 1102 16-17 0.49838721313071943 1102 18-19 0.4912823274170677 1102 20-21 0.5064641215148171 1102 22-23 0.5356275752702189 1102 24-25 0.54842477263864 1102 26-27 0.5953878198464366 1102 28-29 0.554842934220602 1102 30-31 0.5897867609071739 1102 32-33 0.5845235074278676 1102 34-35 0.6031725643715404 1102 36-37 0.6169658525784953 1102 38-39 0.6403299200795161 1102 40-41 0.6251573731743107 1102 42-43 0.5438481426730633 1102 44-45 0.6179349250068213 1102 46-47 0.5925087383340042 1102 48-49 0.6140071814704982 1102 50-51 0.6488809872506707 1102 52-53 0.5655414644281862 1102 54-55 0.5672800352169958 1102 56-57 0.6479573951082145 1102 58-59 0.6379144388712206 1102 60-61 0.6058875924709639 1102 62-63 0.6216024474047686 1102 64-65 0.6928527391680888 1102 66-67 0.618038362676451 1102 68-69 0.6857974038377606 1102 70-71 0.6719244900213965 1102 72-73 0.6293845531807847 1102 74-75 0.674948183571594 1102 76-77 0.6248305493942823 1102 78-79 0.5463108705210686 1102 80-81 0.5234146006481879 1102 82-83 0.49422469007871683 1102 84-85 0.5174558656784214 1102 86-87 0.4675453227748214 1102 88-89 0.5306231490193092 1102 90-91 0.49341657592061594 1102 92-93 0.38585006120618814 1102 94-95 0.4917162957244372 1102 96-97 0.5603186501693926 1102 98-99 0.544173259412922 1102 100-101 0.5362716257659805 1102 102-103 0.5759956366046204 1102 104-105 0.5500413008720066 1102 106-107 0.5953301311217309 1102 108-109 0.5323276930752101 1102 110-111 0.5341104745129464 1102 112-113 0.5467229097753226 1102 114-115 0.5551754232114341 1102 116-117 0.5921316040536411 1102 118-119 0.595905097647897 1102 120-121 0.6490286226606514 1102 122-123 0.6248881176075436 1102 124-125 0.6873379254460978 1103 1 0.25415949527825177 1103 2 0.2816926926497807 1103 3 0.26768477658096046 1103 4 0.29147514269219954 1103 5 0.2944153277868864 1103 6 0.241553386030823 1103 7 0.25658215340193635 1103 8 0.3128002707499533 1103 9 0.3950952547893749 1103 10-11 0.38839867584106 1103 12-13 0.37491831653183283 1103 14-15 0.49979241408045993 1103 16-17 0.463693977429223 1103 18-19 0.434874173540436 1103 20-21 0.44916722121188 1103 22-23 0.4526174450447513 1103 24-25 0.4052128543421105 1103 26-27 0.4571564951645115 1103 28-29 0.4730877052877247 1103 30-31 0.447472072896673 1103 32-33 0.15667979929251885 1103 34-35 0.3546690249336564 1103 36-37 0.35549585171946063 1103 38-39 0.4615048530073196 1103 40-41 0.48369529531732525 1103 42-43 0.48343411611964626 1103 44-45 0.48448789375426315 1103 46-47 0.4560894453980495 1103 48-49 0.39763091838707254 1103 50-51 0.34795397471230416 1103 52-53 0.25684309439417063 1103 54-55 0.3883025426483542 1103 56-57 0.44886023397096864 1103 58-59 0.4071453610135265 1103 60-61 0.3685086649586182 1103 62-63 0.3608691224488041 1103 64-65 0.4169858058455418 1103 66-67 0.44597922697942494 1103 68-69 0.36875719886203484 1103 70-71 0.3228469943999883 1103 72-73 0.3878523681147925 1103 74-75 0.2803174373540074 1103 76-77 0.32035550862561024 1103 78-79 0.28761544676469697 1103 80-81 0.28971777474763627 1103 82-83 0.2687339218669962 1103 84-85 0.3129861140105774 1103 86-87 0.33398526805215667 1103 88-89 0.34051028883686385 1103 90-91 0.30771482583169174 1103 92-93 0.33464673752103025 1103 94-95 0.30319336055035606 1103 96-97 0.3033455919260959 1103 98-99 0.38528877692481345 1103 100-101 0.35083362077221736 1103 102-103 0.2960372110669951 1103 104-105 0.3030335120498293 1103 106-107 0.3310634383290356 1103 108-109 0.31852441780839413 1103 110-111 0.2675625818824017 1103 112-113 0.3200927485535878 1103 114-115 0.40440321449002425 1103 116-117 0.3590620158154678 1103 118-119 0.3458825568490518 1103 120-121 0.3480493935630662 1103 122-123 0.34299977372085166 1103 124-125 0.3552323379547886 1104 1 0.23502248740423326 1104 2 0.2597672333321981 1104 3 0.2566758526964463 1104 4 0.2460263237945668 1104 5 0.26595338552967007 1104 6 0.16997543327492082 1104 7 0.2231207360791032 1104 8 0.28734935211478074 1104 9 0.32864066161351957 1104 10-11 0.32358397767833225 1104 12-13 0.272305193172258 1104 14-15 0.3140842775975159 1104 16-17 0.37752914803289883 1104 18-19 0.5006369031992364 1104 20-21 0.40020344168431876 1104 22-23 0.34535445291876954 1104 24-25 0.3698553740271535 1104 26-27 0.4160236867655627 1104 28-29 0.4814887551564908 1104 30-31 0.49245789966832376 1104 32-33 -0.21315432144238855 1104 34-35 0.351601308398223 1104 36-37 0.4186906023756336 1104 38-39 0.4811814934272718 1104 40-41 0.5339787598842562 1104 42-43 0.5836619376419634 1104 44-45 0.5894170276125337 1104 46-47 0.5562889204636576 1104 48-49 0.5358293435839201 1104 50-51 0.5377324524026008 1104 52-53 0.5881102859952279 1104 54-55 0.48712563976121714 1104 56-57 0.5610177142859314 1104 58-59 0.5229511352917413 1104 60-61 0.6279868801817159 1104 62-63 0.5898811959396184 1104 64-65 0.5687800315673286 1104 66-67 0.4691571797353262 1104 68-69 0.4866837080483819 1104 70-71 0.4860018500430314 1104 72-73 0.4682586673273903 1104 74-75 0.43523187304954547 1104 76-77 0.4885906792292829 1104 78-79 0.4154474677620712 1104 80-81 0.42856291910458566 1104 82-83 0.30586987987224745 1104 84-85 0.33107220324941977 1104 86-87 0.29908448065057414 1104 88-89 0.2650467717765004 1104 90-91 0.2524507838369452 1104 92-93 0.27845776114307697 1104 94-95 0.28863797997292906 1104 96-97 0.3090453294589075 1104 98-99 0.39696961681982756 1104 100-101 0.33969346329190486 1104 102-103 0.36627763101450483 1104 104-105 0.4174093650682025 1104 106-107 0.3640608136571224 1104 108-109 0.3790797983858205 1104 110-111 0.39619775248608136 1104 112-113 0.4122050845115943 1104 114-115 0.40043576042178586 1104 116-117 0.385719233663238 1104 118-119 0.41348255684905055 1104 120-121 0.2949596297835413 1104 122-123 0.4208002986552408 1104 124-125 0.5661530728629245 1105 1 0.1728443479100541 1105 2 0.19304203861792502 1105 3 0.20662639089544754 1105 4 0.19432176441765137 1105 5 0.21276777250788115 1105 6 0.1454589722615296 1105 7 0.13237859903526328 1105 8 0.1836218295804315 1105 9 0.19519062223310613 1105 10-11 0.2890197700997845 1105 12-13 0.26645459371482616 1105 14-15 0.4318616119809917 1105 16-17 0.35611054297469735 1105 18-19 0.35025052007155466 1105 20-21 0.28198592177533754 1105 22-23 0.2877874974623964 1105 24-25 0.4023573780527059 1105 26-27 0.4008786093174095 1105 28-29 0.3755840032531097 1105 30-31 0.3446988027925073 1105 32-33 -0.20400707369581994 1105 34-35 0.2679861513141262 1105 36-37 0.3306226580332776 1105 38-39 0.38163606593098365 1105 40-41 0.3123207569088464 1105 42-43 0.29470284076613495 1105 44-45 0.28588364177198144 1105 46-47 0.28212754827498543 1105 48-49 0.21772037360054242 1105 50-51 0.25896591700398375 1105 52-53 0.25141407526624704 1105 54-55 0.26432900022328454 1105 56-57 0.24281136091233435 1105 58-59 0.3106404675749346 1105 60-61 0.25329269973190804 1105 62-63 0.30623008891240033 1105 64-65 0.354017521722227 1105 66-67 0.37952879083184854 1105 68-69 0.39014830943107626 1105 70-71 0.31355028795324813 1105 72-73 0.3213783312811884 1105 74-75 0.3892477302299042 1105 76-77 0.42844985486593146 1105 78-79 0.3701584155173876 1105 80-81 0.31838526967779757 1105 82-83 0.2641538039118032 1105 84-85 0.2600558735040792 1105 86-87 0.23060127420968612 1105 88-89 0.26411889919402043 1105 90-91 0.2764347866373278 1105 92-93 0.3481029873616812 1105 94-95 0.27369050469507883 1105 96-97 0.29390249231880006 1105 98-99 0.30614203302705434 1105 100-101 0.19328887766137726 1105 102-103 0.20067273034065636 1105 104-105 0.30922677996275283 1105 106-107 0.28950721253696443 1105 108-109 0.22537040834469124 1105 110-111 0.20876710577215363 1105 112-113 0.19197478784580113 1105 114-115 0.20880779157607066 1105 116-117 0.23523014641374118 1105 118-119 0.2598144724874487 1105 120-121 0.25787224026748135 1105 122-123 0.31079644812578877 1105 124-125 0.343291114343625 1106 1 0.3552698060162207 1106 2 0.3792158137738362 1106 3 0.3908634447048591 1106 4 0.3473785845726951 1106 5 0.275232039578043 1106 6 0.31985471823811906 1106 7 0.3355653207268432 1106 8 0.279350193334551 1106 9 0.34156726518870073 1106 10-11 0.4008315065952601 1106 12-13 0.40111970421326504 1106 14-15 0.47915872554704464 1106 16-17 0.4288065402516068 1106 18-19 0.37546592528124734 1106 20-21 0.42399334704709446 1106 22-23 0.40266381148869357 1106 24-25 0.3895033235539671 1106 26-27 0.47853535868984665 1106 28-29 0.4869285027905619 1106 30-31 0.49879165361154776 1106 32-33 0.4586940486943121 1106 34-35 0.47492391617951313 1106 36-37 0.4234632627331507 1106 38-39 0.4643120927963551 1106 40-41 0.5581974770241089 1106 42-43 0.4811300764432218 1106 44-45 0.44521303181863203 1106 46-47 0.4782437049010966 1106 48-49 0.5817330239204068 1106 50-51 0.4017833461986058 1106 52-53 0.4141578149121514 1106 54-55 0.38349409401989476 1106 56-57 0.4366254955687836 1106 58-59 0.5243843634725991 1106 60-61 0.48479529237940966 1106 62-63 0.48029065966201756 1106 64-65 0.476902429043669 1106 66-67 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-2.251997052761908 2216 78-79 -2.062402799617608 2216 80-81 -2.0744778694033243 2216 82-83 -1.8920768119595692 2216 84-85 -1.89831351553007 2216 86-87 -1.9710660488660459 2216 88-89 -1.9534817801790432 2216 90-91 -1.9745806516249438 2216 92-93 -2.095578518609468 2216 94-95 -2.0887520588233386 2216 96-97 -2.066201939074176 2216 98-99 -1.9352638737956696 2216 100-101 -2.017672710557317 2216 102-103 -2.0267142112839167 2216 104-105 -1.9613926062015885 2216 106-107 -1.8457267505664312 2216 108-109 -1.8965449473413472 2216 110-111 -1.7386318585027034 2216 112-113 -1.847358677677775 2216 114-115 -1.9285604438353197 2216 116-117 -1.9983766874859725 2216 118-119 -2.0125110679805616 2216 120-121 -2.1487716903380907 2216 122-123 -2.2804720091983874 2216 124-125 -2.445484926507948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46076.0 3 7758.0 4 4270.0 5 3694.0 6 3974.0 7 4500.0 8 5410.0 9 6080.0 10 6626.0 11 7501.0 12 8163.0 13 8235.0 14 8202.0 15 7486.0 16 6758.0 17 6429.0 18 6126.0 19 6229.0 20 6487.0 21 7095.0 22 8403.0 23 10842.0 24 14572.0 25 18609.0 26 23098.0 27 27532.0 28 33116.0 29 40735.0 30 49208.0 31 59579.0 32 76774.0 33 100740.0 34 139834.0 35 206689.0 36 384070.0 37 749959.0 38 359065.0 39 1591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05497577694873 18.32809113737562 12.832830659793068 24.784102425882583 2 15.801683638688813 19.53968835326738 40.1022101771971 24.556417830846712 3 19.17948592878933 23.605205341918122 29.43400137795636 27.781307351336192 4 12.490595318856796 15.86343610365127 37.567875275738544 34.078093301753384 5 13.716115195530454 37.517164452733425 34.94650056544118 13.82021978629494 6 33.095660138992045 36.088878114732296 17.332599027560057 13.482862718715605 7 28.804279743534263 31.78265976640858 21.160416358232073 18.252644131825082 8 25.977153814144817 35.640879728250546 19.925107008872697 18.45685944873194 9 26.058753825320107 16.123030186418124 19.897807053762147 37.92040893449962 10-11 25.00593495774534 26.14324984249731 28.28489994179978 20.565915257957574 12-13 25.377799867232703 23.543979523807685 28.090049023806362 22.988171585153257 14-15 22.38053104545251 25.56387475235643 25.377984435576057 26.677609766615003 16-17 22.777930321908794 28.51696595825172 26.21495298589882 22.490150733940663 18-19 22.519984101406017 27.7453653849205 27.4768630300328 22.25778748364068 20-21 23.152586310005706 27.122447883654495 27.426244620753454 22.298721185586345 22-23 22.79718108834304 27.05417140662406 27.198689249207025 22.949958255825877 24-25 22.636902196167473 27.21066612855685 27.443417610308273 22.709014064967402 26-27 22.589688824042906 27.53146679160278 27.11005153207791 22.768792852276405 28-29 22.488963196416968 27.657533146005143 27.184839637099934 22.668664020477962 30-31 22.672632174607458 27.30505412445472 27.179633632218952 22.842680068718877 32-33 22.414624273576596 27.43593133735133 27.166068984034446 22.98337540503762 34-35 22.971749511672368 27.498291895744515 26.971652690282 22.558305902301115 36-37 23.022974690595518 27.234445609135875 26.916929399911034 22.825650300357566 38-39 22.61004811089589 27.447862588143863 27.060646392739613 22.881442908220638 40-41 22.94968293610334 27.22089526577663 27.18490384437561 22.64451795374442 42-43 22.697678653892435 27.266080801576614 27.031523379317967 23.00471716521298 44-45 22.731589343855433 27.452934927855033 27.130068268695634 22.6854074595939 46-47 22.827723760877237 27.49357244959191 26.75619297124393 22.922510818286927 48-49 22.705855468843712 27.412244410027164 26.961740843854027 22.920159277275097 50-51 22.698502975024105 27.50240668799079 26.935269849890968 22.863820487094134 52-53 22.92316739243071 27.324746937938382 26.958399217178968 22.793686452451936 54-55 22.64175256160473 27.31627724150322 27.054558004031577 22.987412192860475 56-57 22.609241841280344 27.639708891631752 26.935936894819683 22.815112372268217 58-59 22.768600080485516 27.234407964001022 26.990797009841184 23.006194945672277 60-61 22.581996374617937 27.472258348002125 27.141466115237883 22.804279162142052 62-63 22.548057846670222 27.393480784992256 27.26389412881991 22.794567239517608 64-65 22.427823848478663 27.39272672869475 27.099322450366824 23.08012697245976 66-67 22.39980402959072 27.591291920692974 26.873784078827267 23.135119970889043 68-69 22.586714924134164 27.2846615217245 27.17566746912801 22.95295608501333 70-71 22.583759585593352 27.143066251392607 27.08506477136886 23.188109391645185 72-73 22.584017082582218 27.302425712035195 27.016875597014046 23.096681608368545 74-75 22.433114925205942 27.54778478291232 26.876702787463458 23.142397504418277 76-77 22.54951569407357 27.374156187517723 27.000108764265146 23.07621935414356 78-79 22.623406192059868 27.391141425821075 27.021198857905254 22.964253524213802 80-81 22.648807811422785 27.44669617209462 26.92525153787335 22.979244478609242 82-83 22.623729604753272 27.541847716033267 26.974586808952562 22.8598358702609 84-85 22.66253117174028 27.3932963833259 26.96676247753988 22.977409967393942 86-87 22.60326478388965 27.30736134548078 27.016197476715327 23.073176393914242 88-89 22.6742468674749 27.38494253352592 26.90246200666605 23.038348592333126 90-91 22.646376635432524 27.4606720744237 26.979771015563276 22.9131802745805 92-93 22.672993041678584 27.185179809993294 27.10293937400109 23.03888777432704 94-95 22.544669034814703 27.163817863305002 27.22223227186724 23.069280830013057 96-97 22.698048565760256 27.05151378311445 27.1180099280858 23.13242772303949 98-99 22.480022671614066 27.151878483399035 26.989418897684185 23.378679947302718 100-101 22.47408012029481 27.087785188762055 27.14161882413933 23.296515866803805 102-103 22.471635325047384 26.917257434472862 27.204920951438655 23.406186289041102 104-105 22.348708371276807 27.023906471255827 27.078142872493878 23.54924228497349 106-107 22.499458658358375 27.06639143896124 26.93801323351793 23.496136669162457 108-109 22.484193358467934 26.98867480189131 26.892967471697162 23.63416436794359 110-111 22.394788835971923 27.250810637482026 27.004200460585555 23.3502000659605 112-113 22.441609459288934 27.292039167419034 27.038540530621923 23.22781084267011 114-115 22.345077944874838 27.423071585419944 26.822907935826766 23.408942533878452 116-117 22.320743208675538 27.43576833975579 27.026689861461083 23.21679859010759 118-119 22.25389547852522 27.44281231413926 26.78201703444819 23.521275172887332 120-121 22.298198758521444 27.725521042939548 26.752297792249497 23.223982406289508 122-123 22.334478858976816 27.6950634305872 26.73879942898452 23.231658281451466 124-125 22.358006817339003 27.73825888616263 26.59788367851827 23.3058506179801 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10179.0 1 5389.5 2 567.0 3 524.5 4 469.0 5 405.0 6 362.0 7 337.0 8 331.5 9 316.5 10 294.5 11 274.0 12 302.0 13 342.0 14 364.0 15 407.5 16 476.0 17 573.5 18 734.5 19 963.5 20 1263.0 21 1746.0 22 2536.0 23 3652.5 24 5074.0 25 7059.0 26 10452.0 27 14728.5 28 18301.5 29 22472.0 30 28022.0 31 33808.0 32 41727.0 33 47313.5 34 50684.5 35 55366.0 36 60511.0 37 65717.5 38 68891.0 39 71954.0 40 76047.5 41 80501.0 42 83502.5 43 85594.5 44 88230.5 45 91693.0 46 92674.5 47 91785.0 48 91318.0 49 90601.0 50 89548.5 51 86628.0 52 85106.5 53 85376.0 54 84586.0 55 79508.0 56 72521.0 57 67441.5 58 62753.5 59 55439.5 60 44876.0 61 36098.0 62 29775.0 63 22481.5 64 16406.0 65 12582.0 66 9150.0 67 7595.5 68 5944.0 69 3995.5 70 3044.0 71 2187.5 72 1744.0 73 1421.0 74 1053.5 75 843.0 76 587.5 77 322.0 78 199.5 79 133.5 80 89.0 81 57.5 82 52.5 83 45.0 84 31.5 85 23.0 86 20.0 87 20.5 88 16.5 89 12.0 90 8.5 91 8.0 92 8.0 93 3.5 94 1.5 95 4.0 96 5.5 97 4.5 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47209909711249987 2 0.46473519278661063 3 0.4592324950485836 4 0.45737128846072145 5 0.4648565758249495 6 0.5242533425854182 7 0.5204500073841348 8 0.5289872810806328 9 0.5350564329975743 10-11 0.5510992245646901 12-13 0.5596364982611879 14-15 0.5399522155439073 16-17 0.5344899788186598 18-19 0.5436341677068518 20-21 0.5444838489752236 22-23 0.5537898819145343 24-25 0.5473768113889659 26-27 0.5553476309065492 28-29 0.5366141819895894 30-31 0.5415301950423121 32-33 0.542582181374582 34-35 0.5413885814975834 36-37 0.4901042477994267 38-39 0.455651695417588 40-41 0.4534263397147094 42-43 0.4678506907706406 44-45 0.4724227852147367 46-47 0.47707580168439195 48-49 0.49184407134895 50-51 0.4735152325597862 52-53 0.4731308529383799 54-55 0.46271214214763007 56-57 0.44458560842236444 58-59 0.4630762912626466 60-61 0.45965733568276945 62-63 0.47626658142879974 64-65 0.4761856594032405 66-67 0.4839137128441462 68-69 0.49388735249432025 70-71 0.4893759495693937 72-73 0.5017974804927342 74-75 0.4907313934975106 76-77 0.48826327171795436 78-79 0.4996732773218046 80-81 0.4933613593281853 82-83 0.4952832574352169 84-85 0.49184407134895 86-87 0.48162766562209813 88-89 0.487049441334566 90-91 0.4715124124271955 92-93 0.4715528734399751 94-95 0.46552418253581307 96-97 0.4540534854127934 98-99 0.416242668970247 100-101 0.41385546921624994 102-103 0.40293099576575503 104-105 0.40738170717151223 106-107 0.40614764628173405 108-109 0.41941885847344645 110-111 0.4431087814559086 112-113 0.4430278594303494 114-115 0.43574487713001947 116-117 0.42961503369390835 118-119 0.4724227852147367 120-121 0.46712239254060767 122-123 0.4303433319239414 124-125 0.44011466651021747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2471515.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.41798880168038 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24386830836464 64.18095225985478 2 9.1343079573938 13.595136545688636 3 2.2787514177919235 5.08740292473158 4 0.8787542994080971 2.61580510451121 5 0.43515673944098276 1.6191744681347404 6 0.2415327383859941 1.0784628372268863 7 0.16484004193423213 0.8586945076311145 8 0.10027504208841498 0.5969813567378957 9 0.06859745886326456 0.4594396432959137 >10 0.40446961242521456 5.546550598284617 >50 0.03404150859831253 1.7275387734032743 >100 0.015179988819222232 1.7385900590022856 >500 1.1244324295835182E-4 0.04225738997626122 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.1244324295835182E-4 0.8530135315208057 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10194 0.41245956427535335 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.0461012779610884E-5 0.0 5 0.0 0.0 0.0 4.0461012779610884E-5 4.0461012779610884E-5 6 4.0461012779610884E-5 0.0 0.0 4.0461012779610884E-5 4.0461012779610884E-5 7 4.0461012779610884E-5 0.0 0.0 4.0461012779610884E-5 4.0461012779610884E-5 8 4.0461012779610884E-5 0.0 0.0 4.0461012779610884E-5 4.0461012779610884E-5 9 4.0461012779610884E-5 0.0 0.0 8.092202555922177E-5 4.0461012779610884E-5 10-11 8.092202555922177E-5 0.0 0.0 8.092202555922177E-5 4.0461012779610884E-5 12-13 8.092202555922177E-5 0.0 0.0 8.092202555922177E-5 1.6184405111844353E-4 14-15 1.0115253194902722E-4 0.0 0.0 8.092202555922177E-5 2.427660766776653E-4 16-17 1.6184405111844356E-4 0.0 0.0 1.2138303833883266E-4 3.2368810223688707E-4 18-19 2.0230506389805445E-4 0.0 0.0 1.416135447286381E-4 3.2368810223688707E-4 20-21 2.4276607667766533E-4 0.0 0.0 2.225355702878599E-4 3.64149115016498E-4 22-23 2.4276607667766533E-4 0.0 0.0 2.4276607667766533E-4 3.8437962140630344E-4 24-25 2.832270894572762E-4 0.0 0.0 4.248406341859143E-4 5.259931661349415E-4 26-27 2.832270894572762E-4 0.0 0.0 8.294507619820232E-4 5.259931661349415E-4 28-29 2.832270894572762E-4 0.0 0.0 0.0019825896262009334 5.259931661349415E-4 30-31 2.832270894572762E-4 0.0 0.0 0.006635606095856185 5.259931661349415E-4 32-33 2.832270894572762E-4 0.0 0.0 0.01758031005274093 5.259931661349415E-4 34-35 3.0345759584708167E-4 0.0 0.0 0.03526177263743089 5.259931661349415E-4 36-37 3.2368810223688707E-4 0.0 0.0 0.06710458969498466 5.259931661349415E-4 38-39 3.2368810223688707E-4 0.0 0.0 0.11005395476054161 5.664541789145524E-4 40-41 3.2368810223688707E-4 0.0 0.0 0.15585582122706113 5.664541789145524E-4 42-43 3.2368810223688707E-4 0.0 0.0 0.2028512875705792 5.664541789145524E-4 44-45 3.439186086266925E-4 0.0 0.0 0.26307750509302996 5.866846853043578E-4 46-47 3.64149115016498E-4 0.0 0.0 0.3379708397480897 6.271456980839687E-4 48-49 3.64149115016498E-4 0.0 0.0 0.41911540087759935 7.28298230032996E-4 50-51 3.64149115016498E-4 0.0 0.0 0.5040835277147822 7.28298230032996E-4 52-53 3.64149115016498E-4 0.0 0.0 0.5875545970791195 7.485287364228015E-4 54-55 3.64149115016498E-4 0.0 0.0 0.6831842007837299 8.092202555922178E-4 56-57 3.64149115016498E-4 0.0 0.0 0.7966975721369282 8.092202555922178E-4 58-59 3.64149115016498E-4 0.0 0.0 0.9266381146786485 8.092202555922178E-4 60-61 3.64149115016498E-4 0.0 0.0 1.0691822627012177 8.496812683718286E-4 62-63 3.64149115016498E-4 0.0 0.0 1.2058595638707432 9.306032939310504E-4 64-65 3.64149115016498E-4 0.0 0.0 1.3542300977335762 9.306032939310504E-4 66-67 3.64149115016498E-4 0.0 0.0 1.532501320040542 9.306032939310504E-4 68-69 4.046101277961089E-4 0.0 0.0 1.7391761733187943 9.710643067106613E-4 70-71 4.046101277961089E-4 0.0 0.0 1.966202916025191 0.001051986332269883 72-73 4.046101277961089E-4 0.0 0.0 2.199501115712428 0.0011935998769985212 74-75 4.046101277961089E-4 0.0 0.0 2.439111233393283 0.0013149829153373538 76-77 4.046101277961089E-4 0.0 0.0 2.6981426372083517 0.0013352134217271593 78-79 4.046101277961089E-4 0.0 0.0 3.0044931954691756 0.0013352134217271593 80-81 4.046101277961089E-4 0.0 0.0 3.3402791405271666 0.0013756744345067703 82-83 4.046101277961089E-4 0.0 0.0 3.6875155522017873 0.001456596460065992 84-85 4.046101277961089E-4 0.0 0.0 4.041610105542552 0.0014768269664557974 86-87 4.046101277961089E-4 0.0 0.0 4.412435287667686 0.0015375184856252135 88-89 4.4507114057571975E-4 0.0 0.0 4.828334038029306 0.0015779794984048245 90-91 4.8553215335533066E-4 0.0 0.0 5.284612879144978 0.0015779794984048245 92-93 4.8553215335533066E-4 0.0 0.0 5.7569749728405455 0.0016184405111844356 94-95 4.8553215335533066E-4 4.0461012779610884E-5 0.0 6.236700970862001 0.0016184405111844356 96-97 5.259931661349415E-4 4.0461012779610884E-5 0.0 6.724579862958549 0.0016791320303538519 98-99 5.259931661349415E-4 4.0461012779610884E-5 0.0 7.251321557829915 0.0017398235495232682 100-101 5.259931661349415E-4 4.0461012779610884E-5 0.0 7.837035178827561 0.0017398235495232682 102-103 5.259931661349415E-4 4.0461012779610884E-5 0.0 8.455238993087235 0.0018005150686926843 104-105 6.069151916941633E-4 4.0461012779610884E-5 0.0 9.073260732789404 0.0018207455750824898 106-107 6.069151916941633E-4 4.0461012779610884E-5 0.0 9.689461726916488 0.0018814370942519063 108-109 6.069151916941633E-4 4.0461012779610884E-5 0.0 10.337667382152242 0.0019825896262009334 110-111 6.069151916941633E-4 4.0461012779610884E-5 0.0 11.036004232221938 0.002063511651760155 112-113 6.069151916941633E-4 4.0461012779610884E-5 0.0 11.769238705814045 0.002103972664539766 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2270 0.0 53.745167 1 GTATCAA 3800 0.0 42.128895 1 TCAACGC 4030 0.0 38.10707 4 ATCAACG 4115 0.0 37.316887 3 CAACGCA 4265 0.0 36.007385 5 GTACATG 7815 0.0 33.96388 1 AACGCAG 4540 0.0 33.95881 6 TATCAAC 4725 0.0 33.378307 2 TACATGG 7800 0.0 32.885384 2 ACATGGG 7810 0.0 32.083862 3 ACGCAGA 5245 0.0 29.054396 7 CGCAGAG 5395 0.0 27.694891 8 CATGGGG 5290 0.0 27.567749 4 ATGGGGG 2810 0.0 21.18286 5 CGTACAC 310 8.42374E-9 21.119642 3 GTATAGC 1470 0.0 20.647346 1 ATAGGCG 145 0.0038596475 20.52546 5 GCAGAGT 7070 0.0 20.123144 9 GTGTAGC 2135 0.0 20.069939 1 GAGTACA 5805 0.0 19.991428 1 >>END_MODULE