FastQCFastQC Report
Thu 26 May 2016
SRR1364084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471515
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49290.19943233199070207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.19498162058494486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.10475356208641258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.068.776741
GTATCAA66750.046.0668371
ATCAACG82100.036.522153
TCAACGC82950.036.14794
TGGTATC18500.035.696772
CAACGCA85250.035.382015
GTGGTAT19600.035.2688941
TATCAAC86150.034.8749662
AACGCAG88650.034.025696
ACGCAGA101200.029.7485147
CGCAGAG103650.029.0453418
GTACATG72300.028.7658441
ACATGGG71950.028.2790093
TACATGG72850.028.1752152
CAGAGTA113000.025.00981110-11
GCAGAGT123300.024.2234469
CATGGGG45850.023.7454644
GTACTTT89050.023.21612714-15
AGAGTAC107650.023.2129610-11
GAGTACT94150.022.24401712-13
Produced by FastQC (version 0.11.5)