FastQCFastQC Report
Thu 26 May 2016
SRR1364083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748352
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71640.40975730287722384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.056.5141
GTATCAA31900.049.252431
TCAACGC37800.040.3157654
ATCAACG37950.040.1506423
CAACGCA39200.039.181895
AACGCAG41150.037.4698226
TATCAAC41100.037.3598182
GTACATG78350.037.0676651
TACATGG77550.036.4534572
ACATGGG76800.034.9526183
ACGCAGA49400.030.7301717
CGCAGAG49800.030.2442558
CATGGGA39650.027.474814
CATGGGG41650.027.1559724
GAGTACA54700.023.71831
GCAGAGT62750.022.8641479
GTATAGG5850.022.381091
ATGGGAG20650.021.3335635
CAGAGTA55950.021.12088810-11
TAAGGTG11300.021.0733365
Produced by FastQC (version 0.11.5)