FastQCFastQC Report
Thu 26 May 2016
SRR1364083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748352
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.3279087963979794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50130.28672715791785636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.1681011604070576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30450.069.290661
GTATCAA62550.060.03051
ATCAACG81350.045.928013
TCAACGC81450.045.7281464
TATCAAC82100.045.5809142
CAACGCA84150.044.331645
AACGCAG87100.043.240026
GTGGTAT14800.038.2578541
TGGTATC15050.037.5545582
ACGCAGA100250.037.3933037
CGCAGAG100700.036.989848
GTACATG71550.031.9875011
GCAGAGT115050.031.8589699
TACATGG71100.031.6298942
ACATGGG68800.031.2172243
TAAGGTG13000.029.2911875
AGGTAAG12350.027.4589182
GTAAGGT13250.026.9423664
GGTAAGG13550.026.7834243
AGAGTAC105200.026.72460610-11
Produced by FastQC (version 0.11.5)