FastQCFastQC Report
Thu 26 May 2016
SRR1364082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880747
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117610.40826216255714226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28500.053.4630971
GTATCAA49250.046.527911
ATCAACG51850.042.8217663
CAACGCA52550.042.1432155
TCAACGC53350.041.509824
AACGCAG54350.040.6386646
TATCAAC59900.037.16242
ACGCAGA64350.033.9557157
GTACATG97250.033.9062041
CGCAGAG66700.032.491598
ACATGGG94900.032.0522963
TACATGG100550.031.7871252
CATGGGG55950.026.3872364
GCAGAGT80300.025.8023389
CATGGGA47250.024.9462784
GAGTACA73950.022.2946471
CAGAGTA76450.021.92338610-11
AGAGTAC68900.021.34442510-11
AGTACAT74000.021.1535382
CCGTGCG1754.7651705E-420.4130829
Produced by FastQC (version 0.11.5)