FastQCFastQC Report
Thu 26 May 2016
SRR1364082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880747
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.22664260346361553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.1999828516700703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.1114641445430647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43500.067.6775741
GTATCAA83500.056.0260851
ATCAACG102850.045.003953
TCAACGC105850.043.8408554
CAACGCA107100.043.4402775
TATCAAC109000.042.464732
AACGCAG109850.042.3542566
ACGCAGA126350.036.7328767
CGCAGAG127800.036.1298758
GCAGAGT145950.031.066089
GTACATG90950.030.8620851
TACATGG95050.029.2307552
ACATGGG90400.029.0890223
TGGTATC22300.027.7462142
AGAGTAC136400.026.87212210-11
TAGATCG900.00953576126.4419065
GTGGTAT24600.025.9212381
CAGAGTA142450.025.85614610-11
CATGGGG52850.025.4412674
GAGTACT105950.025.3562412-13
Produced by FastQC (version 0.11.5)