FastQCFastQC Report
Thu 26 May 2016
SRR1364081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300551
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95850.4166393181459572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.050.07911
GTATCAA39500.046.555431
ATCAACG45300.039.1581273
CAACGCA46000.038.6958735
TCAACGC46400.038.2339864
AACGCAG48450.036.2484176
TATCAAC49900.035.8999562
GTACATG81300.033.8921741
TACATGG84850.031.8443182
ACATGGG85450.030.7206483
ACGCAGA57850.029.9474537
CGCAGAG58350.029.5888048
GTGGTAT15200.028.9732421
TGGTATC16000.024.9219932
CATGGGG52100.023.8814054
GCAGAGT71700.022.5849979
CATGGGA40700.021.7943124
CAGAGTA64150.020.51023510-11
GAGTACA62950.020.3259891
AGAGTAC59950.019.66305510-11
Produced by FastQC (version 0.11.5)