FastQCFastQC Report
Thu 26 May 2016
SRR1364081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300551
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.2027340406711262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.1800438242838346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37350.069.725761
GTATCAA66900.050.418831
ATCAACG80400.041.289893
TCAACGC80800.041.2327424
CAACGCA82800.040.021235
TATCAAC84300.039.4502532
AACGCAG84550.039.3336076
TGGTATC17250.038.627312
GTGGTAT17900.038.6195681
ACGCAGA99100.033.445077
CGCAGAG101900.032.467678
GTACATG73450.030.5069261
TACATGG77500.028.4798552
GCAGAGT117700.027.6036629
ACATGGG75500.027.2643223
GAGTACT73850.026.50973112-13
CAGAGTA110450.025.29444110-11
GTACTTT79000.024.43782814-15
AGAGTAC105250.023.71729510-11
TGTACCG4800.022.3098585
Produced by FastQC (version 0.11.5)