FastQCFastQC Report
Thu 26 May 2016
SRR1364080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704064
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112770.41703894582376755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.058.2410161
GTATCAA54200.050.730531
ATCAACG64200.041.072573
TCAACGC64650.040.7927554
CAACGCA65150.040.5718155
AACGCAG66450.039.5996446
TATCAAC72350.037.511212
GTACATG130500.037.2596051
TACATGG134750.035.55512
ACATGGG138450.033.1900253
ACGCAGA84550.030.6317567
CGCAGAG85200.029.7691388
CATGGGG56700.027.5084464
CATGGGA89200.027.4299374
GCAGAGT98150.024.0215599
GAGTACA95650.023.706461
AGTACAT96000.022.5045412
CAGAGTA92100.021.62383710-11
ATGGGAG40500.021.4611875
AGAGTAC85450.021.39058110-11
Produced by FastQC (version 0.11.5)