##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364080_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704064 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14287087879577 31.0 31.0 34.0 28.0 34.0 2 31.12212876618305 33.0 31.0 34.0 28.0 34.0 3 31.228934300371588 33.0 31.0 34.0 28.0 34.0 4 34.691121216065895 37.0 35.0 37.0 32.0 37.0 5 34.73270935895008 37.0 35.0 37.0 32.0 37.0 6 34.77699233450096 37.0 35.0 37.0 32.0 37.0 7 34.676784647108946 37.0 35.0 37.0 32.0 37.0 8 34.60725263899079 37.0 35.0 37.0 31.0 37.0 9 36.11926455882701 39.0 37.0 39.0 31.0 39.0 10-11 36.05291812619819 39.0 37.0 39.0 31.0 39.0 12-13 35.960623158327614 39.0 37.0 39.0 30.0 39.0 14-15 37.22768192616743 40.0 38.0 41.0 31.0 41.0 16-17 37.24973669262266 40.0 38.0 41.0 31.0 41.0 18-19 37.26108220811342 40.0 38.0 41.0 31.0 41.0 20-21 37.289731493041586 40.0 38.0 41.0 31.0 41.0 22-23 37.25628387493787 40.0 37.5 41.0 31.0 41.0 24-25 37.27286798685238 40.0 37.0 41.0 31.0 41.0 26-27 37.25102715764124 40.0 37.0 41.0 31.0 41.0 28-29 37.21874463770088 40.0 37.0 41.0 31.0 41.0 30-31 37.17340972698871 40.0 37.0 41.0 31.0 41.0 32-33 37.0861492183617 40.0 37.0 41.0 30.0 41.0 34-35 37.0604207222906 40.0 37.0 41.0 30.0 41.0 36-37 37.00019211823388 40.0 37.0 41.0 30.0 41.0 38-39 36.9170955273248 40.0 37.0 41.0 30.0 41.0 40-41 36.8360046211924 40.0 37.0 41.0 30.0 41.0 42-43 36.72375838737545 40.0 36.5 41.0 30.0 41.0 44-45 36.62554529034816 40.0 36.0 41.0 30.0 41.0 46-47 36.51128061318075 40.0 36.0 41.0 30.0 41.0 48-49 36.40018967746325 40.0 36.0 41.0 30.0 41.0 50-51 36.275842583607485 40.0 35.5 41.0 29.0 41.0 52-53 36.1352131458427 40.0 35.0 41.0 29.0 41.0 54-55 35.95487643783579 39.0 35.0 41.0 29.0 41.0 56-57 35.773149784916335 39.0 35.0 41.0 28.0 41.0 58-59 35.56889186054768 39.0 35.0 41.0 28.0 41.0 60-61 35.332350121890606 38.5 35.0 41.0 28.0 41.0 62-63 35.07522473580507 38.0 34.0 40.0 28.0 41.0 64-65 34.7964204989231 37.5 34.0 40.0 27.0 41.0 66-67 34.495717187167166 37.0 34.0 40.0 26.5 41.0 68-69 34.174854034519896 36.5 34.0 39.5 26.0 41.0 70-71 33.828693403706424 36.0 34.0 39.0 26.0 41.0 72-73 33.473740451409434 35.5 33.0 39.0 26.0 40.5 74-75 33.197489593441574 35.0 33.0 38.0 26.0 40.0 76-77 32.962203372405384 35.0 33.0 37.0 26.0 39.0 78-79 32.65123199746752 35.0 33.0 37.0 26.0 39.0 80-81 32.43078566187783 35.0 33.0 36.0 25.5 39.0 82-83 32.318449563323945 35.0 33.0 36.0 26.0 37.5 84-85 32.09960063815058 35.0 33.0 36.0 26.0 37.0 86-87 31.895532243319686 35.0 33.0 35.0 25.0 37.0 88-89 31.706876020685904 35.0 33.0 35.0 25.0 36.0 90-91 31.55529621340323 35.0 33.0 35.0 25.0 36.0 92-93 31.429914565631584 35.0 33.0 35.0 24.5 36.0 94-95 31.32217395742113 35.0 33.0 35.0 24.0 36.0 96-97 31.2278916105536 35.0 33.0 35.0 24.0 35.0 98-99 31.140418089216823 35.0 33.0 35.0 23.5 35.0 100-101 31.056087799697046 35.0 33.0 35.0 23.0 35.0 102-103 29.963146027608815 34.0 30.5 35.0 19.0 35.0 104-105 30.7643417463492 35.0 32.0 35.0 20.0 35.0 106-107 30.846130121180565 35.0 33.0 35.0 20.0 35.0 108-109 30.806139388712694 35.0 33.0 35.0 19.0 35.0 110-111 30.71956432983835 35.0 33.0 35.0 18.5 35.0 112-113 30.63074487142316 35.0 32.5 35.0 17.5 35.0 114-115 30.513213444652198 35.0 32.0 35.0 12.5 35.0 116-117 30.40287397043857 35.0 32.0 35.0 4.5 35.0 118-119 30.249800115677736 35.0 32.0 35.0 2.0 35.0 120-121 30.087811531088022 35.0 32.0 35.0 2.0 35.0 122-123 29.898654950474544 35.0 32.0 35.0 2.0 35.0 124-125 29.05482821412511 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5286161640672411 1101 2 0.4947634916529715 1101 3 0.5092643984819709 1101 4 0.6056127993892488 1101 5 0.6110988601923353 1101 6 0.46549806327825394 1101 7 0.5159021144944163 1101 8 0.46377249236532236 1101 9 0.5474664234485189 1101 10-11 0.5960805124218851 1101 12-13 0.6196505703106894 1101 14-15 0.7470656919256768 1101 16-17 0.7406814568767288 1101 18-19 0.6959104291830798 1101 20-21 0.7286625097802215 1101 22-23 0.8243707051828792 1101 24-25 0.7356407673484995 1101 26-27 0.7026582272530106 1101 28-29 0.7429645620127516 1101 30-31 0.740568388490253 1101 32-33 0.8295485473775059 1101 34-35 0.8866740726963727 1101 36-37 0.9093571468182446 1101 38-39 0.8087251138024882 1101 40-41 0.8258053245926789 1101 42-43 0.8379508784592531 1101 44-45 0.8179191628862554 1101 46-47 0.8151075876089777 1101 48-49 0.8572864248710061 1101 50-51 0.8233936266261281 1101 52-53 0.7471575024341419 1101 54-55 0.7878764406500594 1101 56-57 0.7978200482967992 1101 58-59 0.7762471512096454 1101 60-61 0.6986449671729815 1101 62-63 0.7849811239985769 1101 64-65 0.732973648458561 1101 66-67 0.7453766916219493 1101 68-69 0.7175907559969303 1101 70-71 0.7255040879147785 1101 72-73 0.7601918535845513 1101 74-75 0.7892044598676975 1101 76-77 0.7287560046639356 1101 78-79 0.6437615717118561 1101 80-81 0.6600967601137882 1101 82-83 0.6543908375989318 1101 84-85 0.6460060852003764 1101 86-87 0.6496389853409816 1101 88-89 0.6912686715914411 1101 90-91 0.7417899391614107 1101 92-93 0.7302337563230878 1101 94-95 0.7358364789220495 1101 96-97 0.6118655360606517 1101 98-99 0.6322103187313424 1101 100-101 0.7116925345002585 1101 102-103 0.8207613111272636 1101 104-105 0.7576115302708288 1101 106-107 0.7495159999825987 1101 108-109 0.7948903263759881 1101 110-111 0.7900299166533102 1101 112-113 0.8415488964889306 1101 114-115 0.850074670226558 1101 116-117 0.8988986958700735 1101 118-119 0.9241295357075714 1101 120-121 0.9091554276486775 1101 122-123 1.0064737576186609 1101 124-125 1.1067258249646699 1102 1 0.5372042068402969 1102 2 0.5939046484717316 1102 3 0.5683311346742883 1102 4 0.6010915142056703 1102 5 0.5320474988335562 1102 6 0.6173677926871477 1102 7 0.6061207195732194 1102 8 0.6144255013315103 1102 9 0.7220315390975856 1102 10-11 0.6559963593286966 1102 12-13 0.6865907489030789 1102 14-15 0.7528334590591967 1102 16-17 0.7715580804512427 1102 18-19 0.7475173041820256 1102 20-21 0.7979421762646055 1102 22-23 0.7823162041471932 1102 24-25 0.8118821301895025 1102 26-27 0.8329056380991418 1102 28-29 0.8137840136670391 1102 30-31 0.8110582914288429 1102 32-33 0.7420818442794754 1102 34-35 0.8088997652933259 1102 36-37 0.8354061880762558 1102 38-39 0.9069326735365593 1102 40-41 0.8202994133348795 1102 42-43 0.8176387470334703 1102 44-45 0.8878395446946215 1102 46-47 0.8690545988987708 1102 48-49 0.9555667673760269 1102 50-51 0.9713130508790755 1102 52-53 1.0087580711058735 1102 54-55 0.955891074493401 1102 56-57 0.943790515285329 1102 58-59 0.9693942107921885 1102 60-61 1.0285763354514614 1102 62-63 0.9966297584770842 1102 64-65 1.007457480827746 1102 66-67 1.0059729553143484 1102 68-69 0.9749651906417611 1102 70-71 1.0250435431793363 1102 72-73 0.9982951287379223 1102 74-75 0.9144456480300605 1102 76-77 0.9826700967441013 1102 78-79 0.9787561893529997 1102 80-81 0.9229426111975769 1102 82-83 0.8570634274323226 1102 84-85 0.8806539132003834 1102 86-87 0.9238501984135361 1102 88-89 0.9304601545353783 1102 90-91 0.917210908873006 1102 92-93 0.9010243477445385 1102 94-95 0.8854284804294501 1102 96-97 0.8981301919819664 1102 98-99 0.9618094150189584 1102 100-101 0.996555838406227 1102 102-103 0.9821106317284638 1102 104-105 0.9649018546167909 1102 106-107 0.9499459078723547 1102 108-109 0.9192091023338271 1102 110-111 0.9462850592666925 1102 112-113 0.9457413397115637 1102 114-115 0.9718328867043695 1102 116-117 0.9305166986900915 1102 118-119 0.9535026520278649 1102 120-121 0.8574907154722808 1102 122-123 0.877832852184131 1102 124-125 0.9824261767729183 1103 1 0.3838486634450611 1103 2 0.43655401453340303 1103 3 0.43036012930065226 1103 4 0.4105023123289442 1103 5 0.40732568334021835 1103 6 0.28361594693456027 1103 7 0.39284010741516084 1103 8 0.33068620128111803 1103 9 0.41647380591643923 1103 10-11 0.49480706930970797 1103 12-13 0.5338630708404963 1103 14-15 0.5578555823026008 1103 16-17 0.4786285700198647 1103 18-19 0.5210988081809234 1103 20-21 0.5847079174461172 1103 22-23 0.6960278507880133 1103 24-25 0.6513432374811785 1103 26-27 0.7150867309909756 1103 28-29 0.7468619437525277 1103 30-31 0.6540808695667764 1103 32-33 0.4222666877302572 1103 34-35 0.6653716778772463 1103 36-37 0.6903265209332048 1103 38-39 0.6997850788300468 1103 40-41 0.6925656622756549 1103 42-43 0.7705204484112045 1103 44-45 0.7647799702737217 1103 46-47 0.7175524799257929 1103 48-49 0.7303439490001509 1103 50-51 0.6195554469973104 1103 52-53 0.6354957733521331 1103 54-55 0.717831024289957 1103 56-57 0.7180858220322506 1103 58-59 0.7163955558771349 1103 60-61 0.7836919647918563 1103 62-63 0.8406925790500779 1103 64-65 0.6742954313577059 1103 66-67 0.6902935288218472 1103 68-69 0.7432999994597012 1103 70-71 0.7815652130754813 1103 72-73 0.7322007205881889 1103 74-75 0.7946341275425297 1103 76-77 0.7993293902856777 1103 78-79 0.8008029142929232 1103 80-81 0.7892499417042416 1103 82-83 0.6795979906484817 1103 84-85 0.6292657306071732 1103 86-87 0.6688090309746961 1103 88-89 0.6552537371185814 1103 90-91 0.6318908245576402 1103 92-93 0.662779077590308 1103 94-95 0.6466474391645356 1103 96-97 0.7210448654765251 1103 98-99 0.7077554318810009 1103 100-101 0.681398999077345 1103 102-103 0.6593404658982642 1103 104-105 0.6315136150328229 1103 106-107 0.6356133392294403 1103 108-109 0.660133389214451 1103 110-111 0.631251227131024 1103 112-113 0.6078791969933022 1103 114-115 0.6655217344647859 1103 116-117 0.6600827734093535 1103 118-119 0.6739392140980129 1103 120-121 0.6990618345179698 1103 122-123 0.7082103166474312 1103 124-125 0.7096470239391905 1104 1 0.24403025136507495 1104 2 0.29497577426429444 1104 3 0.3053778137036147 1104 4 0.30579776150752025 1104 5 0.30124056574485536 1104 6 0.22796773270425774 1104 7 0.2891823549147574 1104 8 0.34305016286086953 1104 9 0.39190484515977175 1104 10-11 0.3163283500233689 1104 12-13 0.4136849303929395 1104 14-15 0.5902317139071656 1104 16-17 0.5130844925987361 1104 18-19 0.5271463151928799 1104 20-21 0.4739418895918064 1104 22-23 0.4637224107764055 1104 24-25 0.4020809203273856 1104 26-27 0.5623535111884408 1104 28-29 0.5685358757066226 1104 30-31 0.5145133261919952 1104 32-33 -0.21475055436540202 1104 34-35 0.44383899762650003 1104 36-37 0.5050452232214795 1104 38-39 0.6347023999730226 1104 40-41 0.6198145924021432 1104 42-43 0.624471111162137 1104 44-45 0.6055498361036697 1104 46-47 0.5947537607762072 1104 48-49 0.5312362421767816 1104 50-51 0.4909717257466255 1104 52-53 0.47825769842495447 1104 54-55 0.597429429248848 1104 56-57 0.5728321514339072 1104 58-59 0.5579407414331996 1104 60-61 0.5980496488687379 1104 62-63 0.5942970088023714 1104 64-65 0.6477298752101888 1104 66-67 0.6917704394342294 1104 68-69 0.5897477419832029 1104 70-71 0.6563776203474134 1104 72-73 0.6022202930805705 1104 74-75 0.6297048493764663 1104 76-77 0.6789253956880259 1104 78-79 0.6690355403202659 1104 80-81 0.6298567034310381 1104 82-83 0.6282039929320717 1104 84-85 0.631926510098836 1104 86-87 0.5963862730282834 1104 88-89 0.6307236951966892 1104 90-91 0.5443017517042144 1104 92-93 0.6469157736862741 1104 94-95 0.6225815635144194 1104 96-97 0.6292286615144782 1104 98-99 0.5887576431545263 1104 100-101 0.5742212286216848 1104 102-103 0.6203413610314605 1104 104-105 0.7173711284453574 1104 106-107 0.7243075886560462 1104 108-109 0.6720529397755399 1104 110-111 0.7344621033149608 1104 112-113 0.6977519245146553 1104 114-115 0.7822805725903059 1104 116-117 0.7143336239699707 1104 118-119 0.8124880964174501 1104 120-121 0.7367731335497858 1104 122-123 0.7600538860725834 1104 124-125 0.8307982009972719 1105 1 0.40190992977622386 1105 2 0.3259793395123616 1105 3 0.39106462633732875 1105 4 0.37159116269115344 1105 5 0.3844078455434925 1105 6 0.3281219295893294 1105 7 0.3385847560144768 1105 8 0.33718809273111106 1105 9 0.35725487559024316 1105 10-11 0.37734544298130146 1105 12-13 0.4856656519683398 1105 14-15 0.4777120047541743 1105 16-17 0.5444888997210313 1105 18-19 0.5146817647829067 1105 20-21 0.47041418723543416 1105 22-23 0.42653752744821105 1105 24-25 0.5270967858125744 1105 26-27 0.5441680700204117 1105 28-29 0.5428833087617804 1105 30-31 0.5821304922530786 1105 32-33 0.28525641706946914 1105 34-35 0.6111634071897356 1105 36-37 0.6344440266856637 1105 38-39 0.6389246823295025 1105 40-41 0.7164226735894133 1105 42-43 0.7291463929800557 1105 44-45 0.6139408802045239 1105 46-47 0.5824282639192333 1105 48-49 0.5613777566775582 1105 50-51 0.6212944787968979 1105 52-53 0.6665239544668111 1105 54-55 0.6596392628110053 1105 56-57 0.6009161860477406 1105 58-59 0.6603081031633877 1105 60-61 0.6533183404735894 1105 62-63 0.6984207657142463 1105 64-65 0.6845461165651443 1105 66-67 0.7116149257920341 1105 68-69 0.7085180146344641 1105 70-71 0.684409697451116 1105 72-73 0.6677304601836695 1105 74-75 0.6350852339970956 1105 76-77 0.6693550849220955 1105 78-79 0.6312679638323644 1105 80-81 0.6454685909592257 1105 82-83 0.5750461142540431 1105 84-85 0.5358556045413323 1105 86-87 0.5411771356092423 1105 88-89 0.5195299967235592 1105 90-91 0.5231992496030919 1105 92-93 0.5814062630860022 1105 94-95 0.5074641976889076 1105 96-97 0.4305063160711917 1105 98-99 0.44300516648194943 1105 100-101 0.40801106068075654 1105 102-103 0.48846419683860276 1105 104-105 0.4573772795643052 1105 106-107 0.5165762111092675 1105 108-109 0.5235243001832153 1105 110-111 0.4854565175387364 1105 112-113 0.5392478493827184 1105 114-115 0.6085776439999364 1105 116-117 0.5474066799806678 1105 118-119 0.5386071492676798 1105 120-121 0.5840438449325092 1105 122-123 0.5736568499140304 1105 124-125 0.676895804336759 1106 1 0.2425272230991098 1106 2 0.31733055145051026 1106 3 0.34018822422129347 1106 4 0.33596608371567527 1106 5 0.22103451280693065 1106 6 0.2573966262555132 1106 7 0.2969138958686415 1106 8 0.27104021404633727 1106 9 0.3566863758188532 1106 10-11 0.3289647276607184 1106 12-13 0.2697259347048586 1106 14-15 0.3403299795844035 1106 16-17 0.2947167076437722 1106 18-19 0.4002726076586214 1106 20-21 0.422552515420783 1106 22-23 0.4857071888978197 1106 24-25 0.503185608770778 1106 26-27 0.5300819364939358 1106 28-29 0.48257874966068215 1106 30-31 0.4041128502140552 1106 32-33 0.4830009269912594 1106 34-35 0.4489174918445187 1106 36-37 0.4930611132813567 1106 38-39 0.5185616708682659 1106 40-41 0.48841538027394193 1106 42-43 0.34847620123564127 1106 44-45 0.40072768685550386 1106 46-47 0.38834382674266266 1106 48-49 0.39252405152542735 1106 50-51 0.29877310936279144 1106 52-53 0.34002228296868253 1106 54-55 0.26644550824710933 1106 56-57 0.45486929952983957 1106 58-59 0.4647261920892092 1106 60-61 0.4747360315237614 1106 62-63 0.397684757571767 1106 64-65 0.4933250735176671 1106 66-67 0.5173635737195568 1106 68-69 0.4803677927118386 1106 70-71 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2216 76-77 -2.2739336619571944 2216 78-79 -2.283408376476661 2216 80-81 -2.259723513939619 2216 82-83 -2.012474457870155 2216 84-85 -1.9753688104062945 2216 86-87 -2.036787260383921 2216 88-89 -2.1048094635275767 2216 90-91 -2.1271195263809624 2216 92-93 -2.231574160372798 2216 94-95 -2.196281697206306 2216 96-97 -2.1525618408095575 2216 98-99 -2.1467208320683575 2216 100-101 -2.184252006988462 2216 102-103 -2.235266608641549 2216 104-105 -2.139886524640861 2216 106-107 -2.114085468204795 2216 108-109 -2.2040837474242103 2216 110-111 -2.2582025729169466 2216 112-113 -2.2272248124380596 2216 114-115 -2.252170007498254 2216 116-117 -2.4047903231341436 2216 118-119 -2.5298687795081847 2216 120-121 -2.5800725869931327 2216 122-123 -2.666284446860786 2216 124-125 -3.0270710513439063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47865.0 3 8585.0 4 4842.0 5 4181.0 6 4910.0 7 5570.0 8 6547.0 9 6970.0 10 8014.0 11 8565.0 12 9234.0 13 9375.0 14 9831.0 15 8895.0 16 7713.0 17 7289.0 18 7081.0 19 6865.0 20 7150.0 21 7838.0 22 9287.0 23 12090.0 24 16132.0 25 20468.0 26 25284.0 27 29415.0 28 35738.0 29 44299.0 30 52801.0 31 63117.0 32 81491.0 33 107233.0 34 148442.0 35 220748.0 36 412388.0 37 819369.0 38 416470.0 39 1972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51079262333763 18.733459919217893 12.721212549095382 24.034534908349094 2 15.52181730291565 20.143770484433038 40.200745121542056 24.13366709110926 3 18.8897050327343 25.158098456670754 29.519427505434663 26.432769005160285 4 12.295819534301723 16.169851992150434 37.87389276471292 33.660435708834925 5 13.472845197861027 38.57004425975632 34.333525056253585 13.62358548612907 6 31.961281044799094 36.56956689526356 17.852790341973318 13.616361717964029 7 28.58940595296161 32.71574875979966 21.19597544581782 17.498869841420905 8 26.2031119290614 35.71304816611827 19.958247355603888 18.125592549216442 9 26.802105537912734 16.142977877871516 19.949402839732155 37.105513744483595 10-11 24.911012640174963 26.64765840010368 28.117142192531773 20.32418676718958 12-13 25.132960127582216 24.289769768846494 28.266374737055077 22.310895366516213 14-15 22.427118293659674 26.003548058681663 25.494895064175164 26.074438583483495 16-17 22.554967179944036 29.38785594720831 25.952867321010416 22.104309551837247 18-19 22.396411638932786 28.293408943013855 27.480189643952773 21.829989774100586 20-21 23.350633679805078 27.490687467985992 27.264806856180297 21.893871996028636 22-23 22.91526123385534 27.58745913862929 27.01590199965042 22.481377627864944 24-25 22.535248454422906 27.78578533909729 27.1900525263794 22.488913680100403 26-27 22.567528518156674 27.990580538294267 27.091328390297747 22.35056255325131 28-29 22.331044150126793 27.94549835282919 27.29155666936857 22.431900827675445 30-31 22.517025774317155 27.738300415906714 27.370751922894577 22.373921886881554 32-33 22.227434330228007 27.84866492566146 27.326735213633835 22.5971655304767 34-35 22.776486039371395 27.87853254715981 26.94139730109511 22.403584112373682 36-37 22.82409602016407 27.683991665447977 26.824985742698114 22.66692657168984 38-39 22.488903535153327 27.885721737405277 27.004450474676432 22.62092425276497 40-41 22.705878550379612 27.643289479745764 27.260295042062886 22.390536927811734 42-43 22.525297863438727 27.721085528589168 27.11090485238919 22.64271175558292 44-45 22.58282370899503 27.81779712975411 27.07801506986851 22.521364091382353 46-47 22.540380904781237 27.924625407710806 26.793337650043615 22.741656037464338 48-49 22.511540231884283 27.893791772424635 26.950950042470435 22.643717953220648 50-51 22.44797024647027 27.83583685425315 26.876851072384138 22.83934182689244 52-53 22.66739942174774 27.753711802075863 26.88053676910746 22.69835200706893 54-55 22.40992553729238 27.62320677292413 27.020044536809934 22.946823152973558 56-57 22.423907412659787 28.003197739590398 26.897102289961204 22.67579255778861 58-59 22.56932014363296 27.605298049169523 27.033653130745638 22.791728676451875 60-61 22.595467579551055 27.70508504047122 27.076504843691833 22.62294253628589 62-63 22.389980062163836 27.784399363163477 27.050948593974727 22.77467198069796 64-65 22.32087665283279 27.633166295040372 26.98893147577784 23.057025576348998 66-67 22.276753628703492 27.907886786086173 26.810802755651814 23.00455682955852 68-69 22.28579668799126 27.711861855555924 27.15019105169676 22.852150404756056 70-71 22.269617517796775 27.685701240910216 27.06075849775144 22.98392274354157 72-73 22.417210075437925 27.640011388539502 26.959449514277306 22.983329021745273 74-75 22.25471447952811 27.861025637499758 26.89076723331041 22.993492649661718 76-77 22.362367250585702 27.750610974347996 26.894763756585444 22.99225801848086 78-79 22.476028458841206 27.78193647086284 26.857808377829695 22.884226692466257 80-81 22.31713311176378 28.100061230500778 26.84252239930649 22.74028325842895 82-83 22.394378812802767 27.949484242583267 26.807715436596812 22.848421508017154 84-85 22.567618192467673 27.647512019195037 26.723160104300153 23.06170968403714 86-87 22.388146226761293 27.53577490540077 27.002618956263706 23.073459911574226 88-89 22.513899108821846 27.5845467181008 26.942976490437857 22.958577682639493 90-91 22.464238244984696 27.730711021387012 27.04858366840529 22.756467065223003 92-93 22.43124108453652 27.457769639472495 27.06061233233169 23.050376943659288 94-95 22.40629535309369 27.364008527960728 27.05686149346321 23.172834625482373 96-97 22.52109350952791 27.349088132148157 27.04527769351499 23.08454066480894 98-99 22.416672871748297 27.256673681348975 26.852913197623714 23.473740249279018 100-101 22.285043066495664 27.202145609635043 27.133646977472324 23.37916434639697 102-103 22.48518370057605 27.15503100649836 27.05818965717308 23.301595635752502 104-105 22.29384369527934 27.251866316293523 27.06983468059148 23.384455307835662 106-107 22.345034486292747 27.27725761375798 27.055400872053514 23.32230702789576 108-109 22.364676147322733 27.392074330977934 26.73921226113517 23.504037260564164 110-111 22.200633147445643 27.561509360507706 26.939116208782636 23.298741283264015 112-113 22.28759967822875 27.495338458815265 26.969516241702884 23.2475456212531 114-115 22.000855445815233 27.794801467295727 26.786337456844656 23.418005630044387 116-117 22.099835663064898 27.812108785145384 26.86205967349154 23.225995878298182 118-119 22.209928984722882 27.8848342261568 26.539161331184836 23.366075457935484 120-121 22.141062356773748 27.988697027767895 26.67105731279452 23.199183302663833 122-123 22.28103173931066 28.058546793308935 26.553908393839045 23.10651307354136 124-125 22.33739116738988 28.10574006174881 26.42097401761604 23.135894753245267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11862.0 1 6389.5 2 1009.5 3 1128.0 4 1026.0 5 812.0 6 662.5 7 608.5 8 626.0 9 600.5 10 535.5 11 536.0 12 559.5 13 563.0 14 603.5 15 695.5 16 811.5 17 891.5 18 1062.0 19 1348.5 20 1706.0 21 2325.0 22 3275.5 23 4695.0 24 6659.0 25 9280.5 26 13662.0 27 18553.5 28 22379.5 29 26642.5 30 31931.5 31 37917.0 32 43948.5 33 49628.5 34 57178.0 35 63507.0 36 69163.5 37 76429.0 38 80329.5 39 81760.0 40 83648.5 41 87610.5 42 91482.5 43 95129.0 44 98553.5 45 100336.0 46 99426.0 47 98064.5 48 100191.0 49 100206.0 50 97903.0 51 95279.5 52 91512.5 53 88952.0 54 86225.5 55 80260.5 56 72937.0 57 66938.5 58 61801.5 59 55165.0 60 46842.0 61 38616.0 62 32083.5 63 25746.5 64 20490.0 65 16300.0 66 11747.0 67 9620.5 68 7072.0 69 4008.5 70 2877.0 71 2317.0 72 1899.0 73 1579.5 74 1265.0 75 1061.0 76 702.5 77 318.5 78 172.5 79 106.0 80 54.5 81 30.5 82 23.0 83 21.5 84 17.0 85 16.5 86 12.5 87 8.5 88 7.5 89 7.0 90 8.5 91 8.5 92 4.5 93 3.5 94 4.0 95 2.5 96 3.5 97 4.0 98 4.0 99 3.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47609819885919863 2 0.46933060755958445 3 0.4639313270691818 4 0.4604180966130979 5 0.4689238124541431 6 0.5296472272845614 7 0.5242479467941586 8 0.5330495136209794 9 0.5393733284419303 10-11 0.5568840086625169 12-13 0.5653712338169511 14-15 0.5438480746017845 16-17 0.5380789803791627 18-19 0.5478420629097537 20-21 0.5477496094766988 22-23 0.5581968474118956 24-25 0.5524647345624956 26-27 0.5607485621642092 28-29 0.5400759745331472 30-31 0.5458080873825472 32-33 0.5478050815365317 34-35 0.5455122363967717 36-37 0.49323906534756573 38-39 0.46008526425410046 40-41 0.45782940048756243 42-43 0.4707728811152399 44-45 0.4758023478734231 46-47 0.47970388274833736 48-49 0.49438548791744574 50-51 0.4765974473976947 52-53 0.4752476272750941 54-55 0.4653181285650044 56-57 0.4497859518117915 58-59 0.4667234207474379 60-61 0.46308075548507727 62-63 0.47889029253745474 64-65 0.47944501313578375 66-67 0.4866009088542283 68-69 0.4966968237438167 70-71 0.49323906534756573 72-73 0.5044259307472013 74-75 0.49323906534756573 76-77 0.49111263638730446 78-79 0.502281011100329 80-81 0.4959756869659889 82-83 0.497695320820809 84-85 0.49436699723083477 86-87 0.4846963681332986 88-89 0.4897813069513148 90-91 0.4752476272750941 92-93 0.4738423350926605 94-95 0.46899777520058694 96-97 0.459253183356607 98-99 0.42079255520579395 100-101 0.41824084045348037 102-103 0.40677661475468035 104-105 0.4114362677806443 106-107 0.41086305649570426 108-109 0.4237510650635488 110-111 0.44806631795697144 112-113 0.4470863115665902 114-115 0.4400968320276443 116-117 0.4337730172066933 118-119 0.47641254053158505 120-121 0.4710132600411825 122-123 0.43475302359707463 124-125 0.44483044780005204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2704064.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.336434891052896 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77477827055873 49.54436928231359 2 10.78468505707637 13.229882656477516 3 3.6414175795848602 6.7005471684402655 4 1.6361864322797608 4.014313702926067 5 0.88677733908842 2.7195880260929006 6 0.5253436416592236 1.9333623643235758 7 0.347432649653756 1.4917196066159515 8 0.24367612777037728 1.1956979956393303 9 0.17125761965941555 0.9453898654052795 >10 0.8794488698499328 9.84685089655479 >50 0.06840941368005145 2.8732177700879027 >100 0.039806480250194634 4.229690751356779 >500 6.244151111459381E-4 0.1956141692060146 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.5610377778648452E-4 1.0797557445600716 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11277 0.41703894582376755 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.698137322193557E-5 0.0 4 0.0 0.0 0.0 3.698137322193557E-5 0.0 5 0.0 0.0 0.0 3.698137322193557E-5 0.0 6 0.0 0.0 0.0 3.698137322193557E-5 0.0 7 0.0 0.0 0.0 3.698137322193557E-5 0.0 8 0.0 0.0 0.0 3.698137322193557E-5 0.0 9 0.0 0.0 0.0 3.698137322193557E-5 0.0 10-11 0.0 0.0 0.0 7.396274644387114E-5 0.0 12-13 0.0 0.0 0.0 1.1094411966580672E-4 0.0 14-15 0.0 0.0 0.0 1.479254928877423E-4 0.0 16-17 0.0 0.0 0.0 1.479254928877423E-4 0.0 18-19 0.0 0.0 0.0 1.8490686610967788E-4 0.0 20-21 0.0 0.0 0.0 2.4037892594258123E-4 0.0 22-23 0.0 0.0 0.0 3.3283235899742014E-4 0.0 24-25 0.0 0.0 0.0 7.396274644387115E-4 0.0 26-27 0.0 0.0 0.0 0.001479254928877423 0.0 28-29 0.0 0.0 0.0 0.003309832903363234 0.0 30-31 0.0 0.0 0.0 0.010021952143144542 0.0 32-33 0.0 0.0 0.0 0.02446317838631038 0.0 34-35 0.0 0.0 0.0 0.04618973515419753 0.0 36-37 0.0 0.0 0.0 0.08269035052424795 0.0 38-39 0.0 0.0 0.0 0.1333548318382997 0.0 40-41 0.0 0.0 0.0 0.19176691084234693 0.0 42-43 0.0 0.0 0.0 0.24788614470663417 0.0 44-45 0.0 0.0 0.0 0.3200183131760195 0.0 46-47 0.0 0.0 0.0 0.41017890109109845 0.0 48-49 0.0 0.0 0.0 0.5104908759555987 0.0 50-51 0.0 0.0 0.0 0.6162206219971125 0.0 52-53 0.0 0.0 1.8490686610967786E-5 0.7236700018934463 0.0 54-55 0.0 0.0 3.698137322193557E-5 0.838608109867222 0.0 56-57 0.0 0.0 3.698137322193557E-5 0.9774176942557573 0.0 58-59 0.0 0.0 3.698137322193557E-5 1.136567033916357 0.0 60-61 0.0 0.0 3.698137322193557E-5 1.310508922865731 0.0 62-63 0.0 0.0 3.698137322193557E-5 1.4920874653854348 0.0 64-65 0.0 0.0 3.698137322193557E-5 1.6759033809850654 0.0 66-67 0.0 0.0 3.698137322193557E-5 1.8895447740881872 0.0 68-69 0.0 0.0 3.698137322193557E-5 2.1397237639345814 0.0 70-71 0.0 0.0 3.698137322193557E-5 2.421041070033845 0.0 72-73 0.0 0.0 3.698137322193557E-5 2.708219923788786 0.0 74-75 0.0 0.0 3.698137322193557E-5 2.9961938770679986 0.0 76-77 0.0 0.0 3.698137322193557E-5 3.308741952853187 0.0 78-79 0.0 0.0 3.698137322193557E-5 3.6607861352394027 0.0 80-81 0.0 0.0 3.698137322193557E-5 4.0663238739911485 0.0 82-83 0.0 0.0 3.698137322193557E-5 4.497341778892807 0.0 84-85 0.0 0.0 3.698137322193557E-5 4.914325252656742 0.0 86-87 0.0 0.0 3.698137322193557E-5 5.352831885635843 0.0 88-89 0.0 0.0 3.698137322193557E-5 5.836770135618092 0.0 90-91 0.0 0.0 3.698137322193557E-5 6.37190909682611 0.0 92-93 0.0 0.0 3.698137322193557E-5 6.943382257224681 0.0 94-95 0.0 0.0 3.698137322193557E-5 7.513782957799815 0.0 96-97 0.0 0.0 3.698137322193557E-5 8.082519496579962 0.0 98-99 0.0 0.0 3.698137322193557E-5 8.688034011029325 0.0 100-101 0.0 0.0 3.698137322193557E-5 9.368343352820052 0.0 102-103 0.0 0.0 3.698137322193557E-5 10.087705024733143 0.0 104-105 0.0 0.0 3.698137322193557E-5 10.803867807862535 0.0 106-107 0.0 0.0 3.698137322193557E-5 11.517497366926227 0.0 108-109 0.0 0.0 3.698137322193557E-5 12.259121825518921 0.0 110-111 0.0 0.0 3.698137322193557E-5 13.049598678137794 0.0 112-113 0.0 0.0 3.698137322193557E-5 13.883935439397884 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3270 0.0 58.241016 1 GTATCAA 5420 0.0 50.73053 1 ATCAACG 6420 0.0 41.07257 3 TCAACGC 6465 0.0 40.792755 4 CAACGCA 6515 0.0 40.571815 5 AACGCAG 6645 0.0 39.599644 6 TATCAAC 7235 0.0 37.51121 2 GTACATG 13050 0.0 37.259605 1 TACATGG 13475 0.0 35.5551 2 ACATGGG 13845 0.0 33.190025 3 ACGCAGA 8455 0.0 30.631756 7 CGCAGAG 8520 0.0 29.769138 8 CATGGGG 5670 0.0 27.508446 4 CATGGGA 8920 0.0 27.429937 4 GCAGAGT 9815 0.0 24.021559 9 GAGTACA 9565 0.0 23.70646 1 AGTACAT 9600 0.0 22.504541 2 CAGAGTA 9210 0.0 21.623837 10-11 ATGGGAG 4050 0.0 21.461187 5 AGAGTAC 8545 0.0 21.390581 10-11 >>END_MODULE