FastQCFastQC Report
Thu 26 May 2016
SRR1364080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704064
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89450.33079838347021373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80090.296183818134482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45470.16815430404014106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53850.073.2514951
GTATCAA100100.055.2405621
ATCAACG127950.042.638133
TCAACGC129250.042.1632464
CAACGCA131050.041.6295245
TATCAAC133500.040.91012
AACGCAG134650.040.6932536
GTGGTAT23300.036.5699461
TGGTATC22100.036.3421482
GTACATG121250.034.498451
ACGCAGA157800.034.4293867
CGCAGAG157850.034.3053868
TACATGG123800.033.1587072
ACATGGG126650.031.2381693
GCAGAGT176200.030.462549
GAGTACT115350.027.5479412-13
CAGAGTA170200.026.90385610-11
CATGGGG51500.026.2233244
AGAGTAC165400.026.06564110-11
GAGTACA92100.025.361251
Produced by FastQC (version 0.11.5)