##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364080_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704064 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18640794004876 33.0 31.0 34.0 30.0 34.0 2 32.21314621251568 34.0 31.0 34.0 30.0 34.0 3 32.34605985657144 34.0 31.0 34.0 30.0 34.0 4 35.959550513597314 37.0 35.0 37.0 35.0 37.0 5 35.896324199427234 37.0 35.0 37.0 35.0 37.0 6 35.93163179569714 37.0 35.0 37.0 35.0 37.0 7 35.85693644824975 37.0 35.0 37.0 35.0 37.0 8 35.846114958817545 37.0 35.0 37.0 35.0 37.0 9 37.521600080471465 39.0 37.0 39.0 34.0 39.0 10-11 37.44606174262148 39.0 37.0 39.0 34.0 39.0 12-13 37.390744634742376 39.0 37.0 39.0 34.0 39.0 14-15 38.72559543709025 40.0 38.0 41.0 34.0 41.0 16-17 38.708183866949895 40.0 38.0 41.0 34.0 41.0 18-19 38.8415605177984 40.0 38.0 41.0 34.0 41.0 20-21 38.89432960906251 40.0 38.0 41.0 34.5 41.0 22-23 38.860692276514165 40.0 38.0 41.0 34.0 41.0 24-25 38.829132372606566 40.0 38.0 41.0 34.0 41.0 26-27 38.71971632328229 40.0 38.0 41.0 34.0 41.0 28-29 38.64416319288301 40.0 38.0 41.0 34.0 41.0 30-31 38.50975790513834 40.0 38.0 41.0 34.0 41.0 32-33 38.36870521555703 40.0 38.0 41.0 34.0 41.0 34-35 38.25161109352442 40.0 38.0 41.0 34.0 41.0 36-37 38.13577433818135 40.0 38.0 41.0 33.0 41.0 38-39 38.03062741858181 40.0 37.0 41.0 33.0 41.0 40-41 37.892199851778656 40.0 37.0 41.0 33.0 41.0 42-43 37.74685917197226 40.0 37.0 41.0 33.0 41.0 44-45 37.561861886405055 40.0 37.0 41.0 33.0 41.0 46-47 37.28197095187096 40.0 37.0 41.0 31.5 41.0 48-49 37.015617973539094 40.0 37.0 41.0 31.0 41.0 50-51 36.83050863441102 40.0 36.0 41.0 31.0 41.0 52-53 36.67879735834655 40.0 36.0 41.0 31.0 41.0 54-55 36.51022035721048 39.5 35.5 41.0 30.0 41.0 56-57 36.329337989041676 39.0 35.0 41.0 30.0 41.0 58-59 36.12861012165392 39.0 35.0 41.0 30.0 41.0 60-61 35.89713094808407 39.0 35.0 41.0 29.0 41.0 62-63 35.65033464444629 38.0 35.0 40.5 29.0 41.0 64-65 35.44568712131074 38.0 35.0 40.0 29.0 41.0 66-67 35.24463011970131 37.0 35.0 40.0 29.0 41.0 68-69 34.942809785567206 37.0 34.5 39.5 29.0 41.0 70-71 34.62658076879838 36.0 34.0 39.0 29.0 41.0 72-73 34.29705435966012 36.0 34.0 39.0 28.5 41.0 74-75 34.02159860121654 35.5 34.0 38.5 28.5 40.0 76-77 33.81957065365317 35.0 34.0 37.0 29.0 39.5 78-79 33.53454522526094 35.0 34.0 37.0 29.0 39.0 80-81 33.25882911794987 35.0 34.0 36.5 29.0 39.0 82-83 32.99579706693333 35.0 34.0 36.0 29.0 37.5 84-85 32.78448531543632 35.0 34.0 36.0 28.5 37.0 86-87 32.59303940291354 35.0 34.0 35.0 28.0 37.0 88-89 32.44625053253178 35.0 34.0 35.0 28.0 36.0 90-91 32.32152585885541 35.0 34.0 35.0 27.0 36.0 92-93 32.219875713000874 35.0 33.5 35.0 27.0 36.0 94-95 32.13151500852051 35.0 33.0 35.0 27.0 36.0 96-97 32.06614543886535 35.0 33.0 35.0 27.0 35.0 98-99 32.00668845116092 35.0 33.0 35.0 27.0 35.0 100-101 31.95080035087927 35.0 33.0 35.0 27.0 35.0 102-103 30.77550087571892 34.0 31.0 35.0 24.5 35.0 104-105 31.695743148091168 35.0 33.0 35.0 25.5 35.0 106-107 31.843870004556106 35.0 33.0 35.0 26.5 35.0 108-109 31.84047141635701 35.0 33.0 35.0 27.0 35.0 110-111 31.804027937208588 35.0 33.0 35.0 26.0 35.0 112-113 31.751460209521667 35.0 33.0 35.0 25.0 35.0 114-115 31.683627125689334 35.0 33.0 35.0 25.0 35.0 116-117 31.621856953089868 35.0 33.0 35.0 25.0 35.0 118-119 31.53761523395896 35.0 33.0 35.0 25.0 35.0 120-121 31.425845874949708 35.0 33.0 35.0 25.0 35.0 122-123 31.291370877316513 35.0 33.0 35.0 24.5 35.0 124-125 30.465789641073584 34.5 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2248128140705603 1101 2 0.2906501251751976 1101 3 0.22296063630579965 1101 4 0.17787258203392042 1101 5 0.16719354939018416 1101 6 0.2238478028362607 1101 7 0.2642398086670852 1101 8 0.19877625683341194 1101 9 0.26001425570201064 1101 10-11 0.32573212411584507 1101 12-13 0.2772987704782821 1101 14-15 0.37973484340372465 1101 16-17 0.405682949222232 1101 18-19 0.3988541000087693 1101 20-21 0.3690463968464428 1101 22-23 0.32481489438679034 1101 24-25 0.3558930484735612 1101 26-27 0.379463552648005 1101 28-29 0.3806184116633844 1101 30-31 0.37694865898846075 1101 32-33 0.4344904341424538 1101 34-35 0.48393031710619283 1101 36-37 0.44737488255515245 1101 38-39 0.4282155543154218 1101 40-41 0.45449584368897433 1101 42-43 0.46580643125604126 1101 44-45 0.4214634776255508 1101 46-47 0.4482259636219226 1101 48-49 0.4988235952034046 1101 50-51 0.44864272307550834 1101 52-53 0.42795341393594555 1101 54-55 0.47738150309733385 1101 56-57 0.493890705942313 1101 58-59 0.43253412900534727 1101 60-61 0.4085109259603996 1101 62-63 0.4610904753607201 1101 64-65 0.4487341171748014 1101 66-67 0.4419267456656186 1101 68-69 0.47093716314173406 1101 70-71 0.4384233917102449 1101 72-73 0.3878781758212284 1101 74-75 0.3809841977086563 1101 76-77 0.3930778881769754 1101 78-79 0.3866508793264174 1101 80-81 0.27433806328541976 1101 82-83 0.3847669808005634 1101 84-85 0.4033248941561638 1101 86-87 0.43550354343127395 1101 88-89 0.40709317352860097 1101 90-91 0.4064631379491175 1101 92-93 0.39106532690599494 1101 94-95 0.42422158201645743 1101 96-97 0.4434809662079573 1101 98-99 0.4322977993181567 1101 100-101 0.412344068693411 1101 102-103 0.4803790602893514 1101 104-105 0.412609302746457 1101 106-107 0.4289274448048168 1101 108-109 0.45144447268311083 1101 110-111 0.4259319987575516 1101 112-113 0.41545044821339516 1101 114-115 0.40973394326249135 1101 116-117 0.48051382622616146 1101 118-119 0.4485430421378993 1101 120-121 0.5354135227683088 1101 122-123 0.45262328282149156 1101 124-125 0.628147404404487 1102 1 0.2860000738631072 1102 2 0.33259725136126406 1102 3 0.24228784987787577 1102 4 0.23836676829530745 1102 5 0.26663653587379343 1102 6 0.2530352825656905 1102 7 0.24767062444490762 1102 8 0.2556829819355144 1102 9 0.2670375175294879 1102 10-11 0.3406939275879637 1102 12-13 0.3415602513201037 1102 14-15 0.4407652555143642 1102 16-17 0.4399940442472854 1102 18-19 0.4697399152853876 1102 20-21 0.4245756310108888 1102 22-23 0.46952419319141114 1102 24-25 0.4754867799090903 1102 26-27 0.41216668336404894 1102 28-29 0.3980566124447549 1102 30-31 0.5183005809658496 1102 32-33 0.4845062356224332 1102 34-35 0.5162202232349031 1102 36-37 0.5421351325414392 1102 38-39 0.4995114170249977 1102 40-41 0.6317461410771088 1102 42-43 0.6359189919996666 1102 44-45 0.6309004884653575 1102 46-47 0.6289569632986272 1102 48-49 0.6969241302232234 1102 50-51 0.6448205285644093 1102 52-53 0.6321619309660775 1102 54-55 0.6047093036000533 1102 56-57 0.7231686891832751 1102 58-59 0.7310922650298295 1102 60-61 0.6946010653311276 1102 62-63 0.73426450963224 1102 64-65 0.6803172555886334 1102 66-67 0.5816566654988762 1102 68-69 0.6580257459661922 1102 70-71 0.655889039214955 1102 72-73 0.5885864413374406 1102 74-75 0.612881809564918 1102 76-77 0.4925968345859033 1102 78-79 0.4931031870622249 1102 80-81 0.4932105093558192 1102 82-83 0.49367827633648176 1102 84-85 0.4787895400143256 1102 86-87 0.4890302301663212 1102 88-89 0.43804413603130143 1102 90-91 0.5026640329210892 1102 92-93 0.48223484131879957 1102 94-95 0.4705891673648779 1102 96-97 0.5096663761185738 1102 98-99 0.517645697072922 1102 100-101 0.5633533728278799 1102 102-103 0.665136616826814 1102 104-105 0.6006551492136545 1102 106-107 0.5465776979462227 1102 108-109 0.5775823964555293 1102 110-111 0.5976169915531919 1102 112-113 0.6196452191879054 1102 114-115 0.5980590551145362 1102 116-117 0.6238636271612314 1102 118-119 0.6455649332502063 1102 120-121 0.655686711359138 1102 122-123 0.6073169513234298 1102 124-125 0.7182824308714792 1103 1 0.1985238436493404 1103 2 0.22093631127685143 1103 3 0.17107048868903973 1103 4 0.10559397728014375 1103 5 0.11378449676524127 1103 6 0.14232861526636498 1103 7 0.19644483326625561 1103 8 0.20523713888237438 1103 9 0.23881710153308688 1103 10-11 0.22037722661509918 1103 12-13 0.17241988077189063 1103 14-15 0.18401555813268544 1103 16-17 0.3001067570020197 1103 18-19 0.28861095294518435 1103 20-21 0.3019775914412506 1103 22-23 0.35693054526224444 1103 24-25 0.3478930881408786 1103 26-27 0.3994773438327286 1103 28-29 0.3355889437379389 1103 30-31 0.3702937874616623 1103 32-33 0.45762884155948313 1103 34-35 0.42219350540205625 1103 36-37 0.4584969459511328 1103 38-39 0.4518567993040179 1103 40-41 0.41564564703689655 1103 42-43 0.4055983055421635 1103 44-45 0.4120310109596801 1103 46-47 0.42650620614679724 1103 48-49 0.39439170996257644 1103 50-51 0.36855543052742235 1103 52-53 0.35281095121507633 1103 54-55 0.41635034849586816 1103 56-57 0.39857965441066057 1103 58-59 0.5119294849950649 1103 60-61 0.47043418763535527 1103 62-63 0.47261641180824654 1103 64-65 0.5016615243560025 1103 66-67 0.3910704614389715 1103 68-69 0.3847655563546013 1103 70-71 0.4400032920094361 1103 72-73 0.46178443108529166 1103 74-75 0.4058107075390893 1103 76-77 0.3606188840765654 1103 78-79 0.3218898423032286 1103 80-81 0.26713830954881956 1103 82-83 0.3346636284232929 1103 84-85 0.30820022467185026 1103 86-87 0.2271879834102819 1103 88-89 0.19898797254571576 1103 90-91 0.24288660502470805 1103 92-93 0.28949478646325844 1103 94-95 0.2380640218063661 1103 96-97 0.33348268331369724 1103 98-99 0.2915814592094961 1103 100-101 0.3171255289505659 1103 102-103 0.37194600622117235 1103 104-105 0.39997655308417635 1103 106-107 0.3202665753624565 1103 108-109 0.3190572501233895 1103 110-111 0.29971548911187185 1103 112-113 0.26672878181661375 1103 114-115 0.2796227008930181 1103 116-117 0.23228547452812975 1103 118-119 0.2642316422946145 1103 120-121 0.3254014378215686 1103 122-123 0.31079102775086653 1103 124-125 0.3922907724095879 1104 1 0.16387733711439267 1104 2 0.2304442746489741 1104 3 0.17612040472428703 1104 4 0.14294784100005842 1104 5 0.12273030649526362 1104 6 0.10892816885681356 1104 7 0.1338079928024456 1104 8 0.11919811380970202 1104 9 0.18174269608402938 1104 10-11 0.18571744034261428 1104 12-13 0.15763828380588052 1104 14-15 0.14847513461161554 1104 16-17 0.21534232376807694 1104 18-19 0.20925258910352085 1104 20-21 0.20382945084117665 1104 22-23 0.22842998672436465 1104 24-25 0.27361895727997876 1104 26-27 0.2821055011889442 1104 28-29 0.2664103204864574 1104 30-31 0.22688761104258504 1104 32-33 0.2271294787239171 1104 34-35 0.2468660283891424 1104 36-37 0.2892131629562442 1104 38-39 0.25207639488473177 1104 40-41 0.22323954004060198 1104 42-43 0.30949281209603896 1104 44-45 0.36057061455557005 1104 46-47 0.3171361285247656 1104 48-49 0.3430104660534994 1104 50-51 0.3702116175965813 1104 52-53 0.411339783760468 1104 54-55 0.46498377146524206 1104 56-57 0.4180549811842482 1104 58-59 0.3944697462666653 1104 60-61 0.37608618856309306 1104 62-63 0.3468294887471046 1104 64-65 0.36528095363758695 1104 66-67 0.28695511679428165 1104 68-69 0.24481753535105355 1104 70-71 0.20794356001295 1104 72-73 0.3285295950256284 1104 74-75 0.2304266930297274 1104 76-77 0.24137394102692156 1104 78-79 0.2485283975624597 1104 80-81 0.22970003403186467 1104 82-83 0.19711539404986667 1104 84-85 0.2502243091194103 1104 86-87 0.23746659887799382 1104 88-89 0.23131552134809397 1104 90-91 0.2910019538768367 1104 92-93 0.25878240217448223 1104 94-95 0.25635189680116355 1104 96-97 0.3244663989369272 1104 98-99 0.30553547213581567 1104 100-101 0.2122438053931468 1104 102-103 0.20360653089965552 1104 104-105 0.32585040583318303 1104 106-107 0.26936356635800607 1104 108-109 0.2977682530564998 1104 110-111 0.29140237341196595 1104 112-113 0.3427935077833162 1104 114-115 0.3227362234525266 1104 116-117 0.3008090845866498 1104 118-119 0.20120794729836788 1104 120-121 0.23166187424896378 1104 122-123 0.2563930810875519 1104 124-125 0.3512036260384832 1105 1 0.101827252575589 1105 2 0.18991172705158021 1105 3 0.12688231690571428 1105 4 0.09376958872564956 1105 5 0.1256264843857764 1105 6 0.1304572102288688 1105 7 0.12268008216586423 1105 8 0.10698553544045808 1105 9 0.20423904749136312 1105 10-11 0.19250017754428228 1105 12-13 0.2028450690690562 1105 14-15 0.1763789123039956 1105 16-17 0.24118174392476277 1105 18-19 0.27177641859335466 1105 20-21 0.2550306697105995 1105 22-23 0.2519800564449639 1105 24-25 0.2845043995359333 1105 26-27 0.2565349033124349 1105 28-29 0.3238379060353438 1105 30-31 0.2936368803549243 1105 32-33 0.27256900987335086 1105 34-35 0.27393711038960333 1105 36-37 0.40683533719609244 1105 38-39 0.3782192881665125 1105 40-41 0.35440796252931506 1105 42-43 0.4417558436460709 1105 44-45 0.43197724003221083 1105 46-47 0.48127681195546756 1105 48-49 0.42281298124716926 1105 50-51 0.4002593772400189 1105 52-53 0.4083088352164168 1105 54-55 0.2668083866701707 1105 56-57 0.34644402701552934 1105 58-59 0.41860266545717195 1105 60-61 0.3282605822264557 1105 62-63 0.3137889515102046 1105 64-65 0.34533486565045024 1105 66-67 0.33838007808250126 1105 68-69 0.2732398252354429 1105 70-71 0.2564645591627439 1105 72-73 0.33545917002820147 1105 74-75 0.3128487795858135 1105 76-77 0.25206887241237297 1105 78-79 0.26208224248697576 1105 80-81 0.3226458923055304 1105 82-83 0.3240954534952536 1105 84-85 0.2954659515569489 1105 86-87 0.2778212029448852 1105 88-89 0.31035858780906267 1105 90-91 0.3277619548222006 1105 92-93 0.26960353841457874 1105 94-95 0.2864699960341355 1105 96-97 0.3605017457998798 1105 98-99 0.29703710545234685 1105 100-101 0.33782370060029976 1105 102-103 0.3838630146779707 1105 104-105 0.3228643183162312 1105 106-107 0.28180182539251675 1105 108-109 0.2552605072608962 1105 110-111 0.2915248580838714 1105 112-113 0.2486660381915904 1105 114-115 0.2763477330851778 1105 116-117 0.20991892426365766 1105 118-119 0.220712427711387 1105 120-121 0.2916505398838183 1105 122-123 0.32491562708307953 1105 124-125 0.368690360617137 1106 1 0.0820878969857759 1106 2 0.10330070033208472 1106 3 0.05360544119648125 1106 4 0.08771354538020404 1106 5 0.05266921979203687 1106 6 0.06531440649877851 1106 7 0.07602625599425039 1106 8 0.101002069164565 1106 9 0.09884885857584891 1106 10-11 0.12186006262770377 1106 12-13 0.1562755040097059 1106 14-15 0.13347554020272412 1106 16-17 0.11077144485530255 1106 18-19 0.18882762833069222 1106 20-21 0.17744046580983053 1106 22-23 0.16272175947641188 1106 24-25 0.23133279396052586 1106 26-27 0.20764182108175078 1106 28-29 0.2545437433830813 1106 30-31 0.26556834781876404 1106 32-33 0.20564071131972383 1106 34-35 0.28208076452018105 1106 36-37 0.2626030010576841 1106 38-39 0.2635548130718135 1106 40-41 0.29766474910351803 1106 42-43 0.24619538422703613 1106 44-45 0.2296637748468271 1106 46-47 0.2092047279648881 1106 48-49 0.21776409001954278 1106 50-51 0.20022015842151575 1106 52-53 0.23045136965713908 1106 54-55 0.15266419148805 1106 56-57 0.20958728920519576 1106 58-59 0.21804384674973676 1106 60-61 0.26145196783522806 1106 62-63 0.19180253813544823 1106 64-65 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-0.5801976817134538 2216 102-103 -0.6945776107508763 2216 104-105 -0.6148495264840577 2216 106-107 -0.7023050688459556 2216 108-109 -0.6772765276005259 2216 110-111 -0.6743313100470338 2216 112-113 -0.6638721815758295 2216 114-115 -0.722650386581865 2216 116-117 -0.7814595748110911 2216 118-119 -0.8376064587026804 2216 120-121 -0.980018629930342 2216 122-123 -1.121535992580636 2216 124-125 -1.5228960813983399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 9.0 4 34.0 5 159.0 6 453.0 7 1070.0 8 1948.0 9 3498.0 10 5861.0 11 8453.0 12 10604.0 13 15113.0 14 22200.0 15 20748.0 16 8947.0 17 6936.0 18 6585.0 19 6045.0 20 5907.0 21 6189.0 22 6506.0 23 7562.0 24 9912.0 25 13174.0 26 17645.0 27 23485.0 28 30541.0 29 39782.0 30 50349.0 31 63551.0 32 82229.0 33 107819.0 34 145962.0 35 216449.0 36 414619.0 37 876792.0 38 464538.0 39 2384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.606022620016034 19.070802754230694 13.48914414074018 23.83403048501309 2 15.945025595205955 20.50338347838886 39.278933381459176 24.27265754494601 3 18.97606713450569 25.119560779626514 30.176504698113654 25.727867387754138 4 12.610426380440702 16.667578873872806 37.37437427516508 33.347620470521406 5 13.014817696622567 38.257193616719135 34.605061122813666 14.122927563844643 6 31.635493834465457 37.19420102482782 17.09367825613595 14.076626884570778 7 27.571277898747958 33.33848607133559 21.26266242219119 17.82757360772526 8 26.27630115263544 35.39661043525597 19.60537916262337 18.72170924948522 9 26.669228243118503 15.373711568956946 20.05895570519041 37.898104482734134 10-11 24.849706809938287 26.454522996633195 27.8800709159009 20.815699277527617 12-13 25.563442881629143 24.08972702027538 28.483173317410333 21.86365678068514 14-15 22.52977370358098 25.38179569714326 26.5134442084211 25.574986390854654 16-17 21.885172241637544 28.904402655276346 26.946709565281708 22.263715537804405 18-19 21.700614334571963 26.86055137748219 29.848054631843034 21.590779656102814 20-21 22.54256925871577 26.01354849589359 30.015580252538403 21.42830199285224 22-23 22.07808321104826 26.11591293697191 29.841841761141747 21.964162090838087 24-25 21.843085074909467 26.354664682492725 29.9658403055549 21.836409937042912 26-27 21.865902582187406 26.575794803673286 29.876900102956146 21.68140251118317 28-29 21.594089489006176 26.49443578258503 30.104446492390714 21.807028236018084 30-31 21.87170865778325 26.263505597500654 30.0860482592128 21.778737485503303 32-33 21.64373698255663 26.353259390310292 30.061048850914773 21.941954776218314 34-35 22.20250704125346 26.387633576720077 29.59336391446356 21.8164954675629 36-37 22.230631375588743 26.28197779342501 29.4121921670493 22.07519866393695 38-39 21.927421096542094 26.422063235189704 29.552906292158767 22.097609376109443 40-41 22.150363305010533 26.2158735887908 29.741049028425365 21.892714077773306 42-43 22.094114636339967 26.301134144753966 29.419237118648077 22.185514100257983 44-45 22.231999686397955 26.489702166812616 29.151621411327543 22.126676735461885 46-47 22.300230319992426 26.704508473172233 28.53536380795721 22.459897398878134 48-49 22.458584560128756 26.8034151558543 28.293616571205416 22.44438371281153 50-51 22.3780761106246 26.884718704882726 28.048337613310924 22.68886757118175 52-53 22.56838225722468 26.938119807815198 27.933639884263094 22.559858050697024 54-55 22.3146900369222 26.864508384416936 27.97735186741142 22.843449711249438 56-57 22.328428617074152 27.18535877849045 27.809844737402667 22.67636786703273 58-59 22.41590805543064 26.819779413505007 28.039739444036826 22.724573087027526 60-61 22.482400564483683 26.83799273981681 28.177402605855484 22.502204089844028 62-63 22.315725515372417 26.78782750704125 28.186426060921637 22.710020916664693 64-65 22.2116784218125 26.661905931220563 28.176367127405268 22.950048519561665 66-67 22.14113645239166 26.96909170788857 27.967607275567442 22.92216456415233 68-69 22.191431119973494 26.74350533123476 28.203825797022557 22.86123775176919 70-71 22.168890972994724 26.68858799200019 28.132710616316775 23.009810418688314 72-73 22.308162824548532 26.67044862843483 28.00967358760739 23.011714959409243 74-75 22.13577415327448 26.888509295637974 27.795218604293385 23.18049794679416 76-77 22.145500254431848 26.842966734515162 27.840280407564315 23.171252603488675 78-79 22.188176759129963 26.83590329222977 27.807089625097632 23.16883032354264 80-81 22.014530721166363 27.110600932522306 27.67942992473551 23.19543842157582 82-83 22.13181714633973 27.002282490355256 27.625326175711816 23.240574187593193 84-85 22.242872210125203 26.72274029017065 27.50452652008237 23.529860979621784 86-87 22.028139866512035 26.52559258952451 27.764061797353907 23.682205746609547 88-89 22.078785857139476 26.662460651818893 27.67035099760952 23.58840249343211 90-91 22.04805433599205 26.74076870961634 27.794793318501338 23.416383635890277 92-93 21.98021200681641 26.440942226219498 27.82826146126719 23.750584305696908 94-95 21.929987086008957 26.30436117163774 27.777136762567473 23.988514979785833 96-97 21.99825436362863 26.304632038714665 27.766676467379348 23.93043713027736 98-99 21.836344832546935 26.194560532900095 27.623722885735987 24.345371748816984 100-101 21.67096806523604 26.15190541447367 27.849856072497964 24.327270447792326 102-103 21.83604933760586 26.019479341546404 27.7574422375632 24.38702908328453 104-105 21.646178692074926 25.978806028167273 27.86319545089479 24.51181982886301 106-107 21.58333094451878 25.983028012010035 27.86440903656181 24.569232006909374 108-109 21.601914363385024 26.06866361884667 27.500570587710893 24.828851430057412 110-111 21.4444034908264 26.121832718998238 27.673115905992766 24.760647884182593 112-113 21.45981289258913 25.973850387313302 27.708039640936654 24.858297079160913 114-115 21.108575106629328 26.180339512223995 27.55618154175098 25.154903839395697 116-117 21.127015073365605 26.10018811882836 27.702962016124843 25.069834791681195 118-119 21.140596779640266 26.138560613934477 27.401538165068867 25.319304441356394 120-121 21.049337517401508 26.234825062018448 27.526869111992745 25.188968308587295 122-123 21.121012973601555 26.292607310725817 27.327414408109945 25.25896530756268 124-125 20.978167818906797 26.205922260916875 27.311725109033304 25.504184811143027 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 599.0 1 409.5 2 332.0 3 451.5 4 311.0 5 242.0 6 561.0 7 1272.5 8 2109.0 9 2418.5 10 1897.0 11 1352.0 12 1374.5 13 1585.5 14 1808.5 15 2064.0 16 2372.0 17 2744.0 18 3158.0 19 3523.5 20 3926.5 21 4504.0 22 5422.5 23 6764.0 24 8553.0 25 11085.0 26 15237.0 27 19862.0 28 23517.0 29 27429.5 30 32298.0 31 38170.5 32 44242.5 33 49281.5 34 55688.0 35 61594.5 36 67619.0 37 75038.5 38 78892.0 39 80504.5 40 82863.5 41 86548.0 42 90088.5 43 93693.5 44 96982.5 45 98968.0 46 98769.0 47 98600.0 48 100579.0 49 99985.5 50 97811.5 51 95224.0 52 91444.5 53 89000.5 54 86159.0 55 80201.5 56 73018.0 57 66784.5 58 61597.5 59 54970.5 60 46781.5 61 38547.5 62 31857.0 63 25418.0 64 20024.5 65 15919.5 66 11311.5 67 9174.0 68 6679.0 69 3738.5 70 2648.0 71 2067.0 72 1751.0 73 1520.0 74 1259.5 75 1014.0 76 616.0 77 272.5 78 127.0 79 62.5 80 29.0 81 12.5 82 10.0 83 8.0 84 2.5 85 1.0 86 0.5 87 1.0 88 1.0 89 0.5 90 1.5 91 1.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16671203048448557 2 0.0015532176753212943 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.917019715509692E-4 12-13 0.008080430048992924 14-15 0.0 16-17 5.17739225107098E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.396274644387115E-4 96-97 0.006619665806726468 98-99 0.0024962426924806515 100-101 0.0036426652623606543 102-103 0.004530218219687108 104-105 0.01196347423729616 106-107 0.018176344938581335 108-109 0.026293756360796193 110-111 0.01714086648836714 112-113 0.025776017135689097 114-115 0.028623582873778135 116-117 0.026608098033182648 118-119 0.016752562069536816 120-121 0.02346468130931812 122-123 0.015532176753212942 124-125 0.015439723320158104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2704064.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.62929134407775 #Duplication Level Percentage of deduplicated Percentage of total 1 79.66731486463667 45.911708989345215 2 11.245942991348386 12.961914501746108 3 3.7856313999964177 6.544897646150474 4 1.799120554464001 4.147281703852984 5 0.9843540916939943 2.836381436798411 6 0.5811340311886812 2.0094205435998527 7 0.39345571905784904 1.5872201985204868 8 0.2574611404251488 1.1869842457071529 9 0.19006811160924944 0.9858141521233309 >10 0.9602721428833739 10.148546335550671 >50 0.08270943483646986 3.264632212962912 >100 0.04947363268700793 5.1440144301209365 >500 0.0020949740655638255 0.811318171659507 >1k 6.446074047888694E-4 0.8853360955162638 >5k 3.223037023944347E-4 1.5745293363456137 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8945 0.33079838347021373 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8009 0.296183818134482 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4547 0.16815430404014106 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.698137322193557E-5 0.0 12-13 0.0 0.0 0.0 7.396274644387114E-5 0.0 14-15 0.0 0.0 0.0 1.1094411966580672E-4 0.0 16-17 0.0 0.0 0.0 1.1094411966580672E-4 0.0 18-19 0.0 0.0 0.0 1.479254928877423E-4 1.8490686610967786E-5 20-21 0.0 0.0 0.0 2.0339755272064566E-4 3.698137322193557E-5 22-23 0.0 0.0 0.0 2.9585098577548463E-4 3.698137322193557E-5 24-25 0.0 0.0 0.0 7.026460912167759E-4 3.698137322193557E-5 26-27 0.0 0.0 0.0 0.0013683108092116163 3.698137322193557E-5 28-29 0.0 0.0 0.0 0.0032728515301412983 3.698137322193557E-5 30-31 0.0 0.0 0.0 0.010077424202977445 3.698137322193557E-5 32-33 0.0 0.0 0.0 0.024592613192587157 3.698137322193557E-5 34-35 0.0 0.0 0.0 0.0462636979006414 3.698137322193557E-5 36-37 0.0 0.0 0.0 0.08302318288324537 3.698137322193557E-5 38-39 0.0 0.0 0.0 0.13433483822868098 3.698137322193557E-5 40-41 0.0 0.0 0.0 0.19341258195072308 3.698137322193557E-5 42-43 0.0 0.0 0.0 0.24995710160706253 3.698137322193557E-5 44-45 0.0 0.0 0.0 0.3221632328228917 3.698137322193557E-5 46-47 0.0 0.0 0.0 0.41258269035052425 3.698137322193557E-5 48-49 0.0 0.0 0.0 0.5131720255141891 3.698137322193557E-5 50-51 0.0 0.0 0.0 0.6188278088092589 3.698137322193557E-5 52-53 0.0 0.0 0.0 0.7264990769449244 3.698137322193557E-5 54-55 0.0 0.0 0.0 0.8416220917848098 3.698137322193557E-5 56-57 0.0 0.0 0.0 0.9813562105038933 3.698137322193557E-5 58-59 0.0 0.0 0.0 1.141614991361151 3.698137322193557E-5 60-61 0.0 0.0 0.0 1.317313495538567 3.698137322193557E-5 62-63 0.0 0.0 0.0 1.5012403552578637 3.698137322193557E-5 64-65 0.0 0.0 0.0 1.685980805188043 3.698137322193557E-5 66-67 0.0 0.0 0.0 1.901212397339708 3.698137322193557E-5 68-69 0.0 0.0 0.0 2.15288913280159 3.698137322193557E-5 70-71 0.0 0.0 0.0 2.435963054128896 3.698137322193557E-5 72-73 0.0 0.0 0.0 2.7260264549951483 3.698137322193557E-5 74-75 0.0 0.0 0.0 3.016459669593619 3.698137322193557E-5 76-77 0.0 0.0 0.0 3.3311896463989017 3.698137322193557E-5 78-79 0.0 0.0 0.0 3.686007431776763 3.698137322193557E-5 80-81 0.0 0.0 0.0 4.09463311519254 3.698137322193557E-5 82-83 0.0 0.0 0.0 4.529718971148612 3.698137322193557E-5 84-85 0.0 0.0 0.0 4.950234166055241 3.698137322193557E-5 86-87 0.0 0.0 0.0 5.391403457906321 3.698137322193557E-5 88-89 0.0 0.0 0.0 5.880038342287756 3.698137322193557E-5 90-91 0.0 0.0 0.0 6.418209776099975 3.698137322193557E-5 92-93 0.0 0.0 0.0 6.992752390475966 3.698137322193557E-5 94-95 0.0 0.0 0.0 7.568385955362004 3.698137322193557E-5 96-97 0.0 0.0 0.0 8.14303951385766 3.698137322193557E-5 98-99 0.0 0.0 0.0 8.75421217840998 3.698137322193557E-5 100-101 0.0 0.0 0.0 9.440697409528768 3.698137322193557E-5 102-103 0.0 0.0 0.0 10.165791194291259 3.698137322193557E-5 104-105 0.0 0.0 0.0 10.887186841731555 3.698137322193557E-5 106-107 0.0 0.0 0.0 11.607417575915363 3.698137322193557E-5 108-109 0.0 0.0 0.0 12.356271892972948 3.698137322193557E-5 110-111 0.0 0.0 0.0 13.153923131996876 3.698137322193557E-5 112-113 0.0 0.0 0.0 13.996802590471232 3.698137322193557E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5385 0.0 73.251495 1 GTATCAA 10010 0.0 55.240562 1 ATCAACG 12795 0.0 42.63813 3 TCAACGC 12925 0.0 42.163246 4 CAACGCA 13105 0.0 41.629524 5 TATCAAC 13350 0.0 40.9101 2 AACGCAG 13465 0.0 40.693253 6 GTGGTAT 2330 0.0 36.569946 1 TGGTATC 2210 0.0 36.342148 2 GTACATG 12125 0.0 34.49845 1 ACGCAGA 15780 0.0 34.429386 7 CGCAGAG 15785 0.0 34.305386 8 TACATGG 12380 0.0 33.158707 2 ACATGGG 12665 0.0 31.238169 3 GCAGAGT 17620 0.0 30.46254 9 GAGTACT 11535 0.0 27.54794 12-13 CAGAGTA 17020 0.0 26.903856 10-11 CATGGGG 5150 0.0 26.223324 4 AGAGTAC 16540 0.0 26.065641 10-11 GAGTACA 9210 0.0 25.36125 1 >>END_MODULE