FastQCFastQC Report
Thu 26 May 2016
SRR1364079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112041
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88600.41949943206594953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.057.4069941
GTATCAA35900.045.252031
ATCAACG39500.040.3803063
TCAACGC40300.039.5834274
CAACGCA40950.038.9588245
AACGCAG42650.037.267276
TATCAAC46900.034.5116652
GTACATG75900.032.3016931
TACATGG76700.031.6544442
ACATGGG76150.030.7934423
ACGCAGA51100.030.4078667
CGCAGAG51850.029.7383848
TCGCGAG450.00952944326.44577872-73
TATACCG1451.33624E-424.6324845
CATGGGA45150.024.2573684
GCAGAGT60150.024.2491669
CATGGGG37950.024.1541944
GAGTACA54850.023.1085591
TGGTATC14450.021.4143732
GTGGTAT17050.021.2899841
Produced by FastQC (version 0.11.5)