FastQCFastQC Report
Thu 26 May 2016
SRR1364079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112041
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53580.2536882569987988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50500.23910520676445202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.1303478483608983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.070.729051
GTATCAA63150.051.255851
ATCAACG77000.041.4139943
TCAACGC78100.040.83074
CAACGCA78300.040.726415
AACGCAG81600.039.152296
TATCAAC81600.039.008322
TGGTATC16450.033.2748072
GTGGTAT16950.032.706211
ACGCAGA98300.032.5046357
CGCAGAG97350.032.3939868
GTACATG66550.031.6186711
TACATGG68700.029.0988732
ACATGGG68250.028.5919823
GCAGAGT111000.027.9815679
GAGTACT77550.026.0868512-13
CAGAGTA109250.025.08029710-11
CATGGGG34600.024.0727144
AGAGTAC103400.023.85220110-11
GTACTTT85050.023.67867914-15
Produced by FastQC (version 0.11.5)