FastQCFastQC Report
Thu 26 May 2016
SRR1364078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910516
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80090.419206120231393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29870.15634519679500197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.054.522441
GTATCAA41900.048.291541
GTACATG105050.042.2052421
TACATGG109450.039.9125332
ATCAACG49400.038.438053
ACATGGG111200.037.6312033
TCAACGC50400.037.56424
CAACGCA52600.035.9930765
AACGCAG53150.035.6206176
TATCAAC55850.035.378872
CATGGGG49050.033.136094
CATGGGA68800.032.1908264
ACGCAGA62950.029.7930747
CGCAGAG63450.029.276798
GAGTACA75000.026.8201731
ATGGGGA28850.026.6208255
ATGGGAG26350.026.209275
AGTACAT73700.025.7603782
GCAGAGT68600.025.516659
ATGGGAT31150.025.0374955
Produced by FastQC (version 0.11.5)