##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364078_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1910516 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.974779064922775 31.0 31.0 34.0 28.0 34.0 2 31.015651792500037 33.0 31.0 34.0 27.0 34.0 3 31.109833678440797 33.0 31.0 34.0 27.0 34.0 4 34.52483308174336 37.0 35.0 37.0 31.0 37.0 5 34.61523693075588 37.0 35.0 37.0 32.0 37.0 6 34.62674638684 37.0 35.0 37.0 31.0 37.0 7 34.53591124073287 37.0 35.0 37.0 31.0 37.0 8 34.43473909666289 37.0 35.0 37.0 30.0 37.0 9 35.910215878851574 39.0 37.0 39.0 30.0 39.0 10-11 35.85175130697675 39.0 37.0 39.0 30.0 39.0 12-13 35.74691209076501 39.0 37.0 39.0 30.0 39.0 14-15 36.99990421435885 40.0 37.0 41.0 30.0 41.0 16-17 37.02537822242787 40.0 37.0 41.0 30.0 41.0 18-19 37.01997339985637 40.0 37.0 41.0 30.0 41.0 20-21 37.043130494588894 40.0 37.0 41.0 30.0 41.0 22-23 37.01057463009993 40.0 37.0 41.0 30.0 41.0 24-25 37.02491552020501 40.0 37.0 41.0 30.0 41.0 26-27 36.9970623119618 40.0 37.0 41.0 30.0 41.0 28-29 36.952007991558304 40.0 37.0 41.0 30.0 41.0 30-31 36.89867763473323 40.0 37.0 41.0 30.0 41.0 32-33 36.80676398418019 40.0 37.0 41.0 30.0 41.0 34-35 36.771277236097475 40.0 37.0 41.0 30.0 41.0 36-37 36.70161045497656 40.0 37.0 41.0 30.0 41.0 38-39 36.60762406595914 40.0 36.0 41.0 30.0 41.0 40-41 36.513053018137505 40.0 36.0 41.0 30.0 41.0 42-43 36.38742334531614 40.0 36.0 41.0 30.0 41.0 44-45 36.27486239319639 40.0 36.0 41.0 29.0 41.0 46-47 36.13989309694344 40.0 35.0 41.0 29.0 41.0 48-49 36.01353142292449 40.0 35.0 41.0 28.0 41.0 50-51 35.86770144819515 39.5 35.0 41.0 28.0 41.0 52-53 35.70832905874643 39.0 35.0 41.0 28.0 41.0 54-55 35.50844588582352 39.0 35.0 41.0 28.0 41.0 56-57 35.300547600752886 39.0 35.0 41.0 27.0 41.0 58-59 35.0823879517366 38.5 34.5 41.0 27.0 41.0 60-61 34.834271997722084 38.0 34.0 40.0 26.5 41.0 62-63 34.558630757345135 37.5 34.0 40.0 26.0 41.0 64-65 34.270346074044916 37.0 34.0 40.0 26.0 41.0 66-67 33.957936494643334 36.5 34.0 39.5 26.0 41.0 68-69 33.635702344288134 36.0 33.0 39.0 26.0 41.0 70-71 33.29299257373401 35.5 33.0 39.0 25.0 41.0 72-73 32.941056238209995 35.0 33.0 38.5 25.0 40.0 74-75 32.67950674058736 35.0 33.0 37.0 24.5 39.5 76-77 32.45863866096908 35.0 33.0 37.0 24.5 39.0 78-79 32.16928515647082 35.0 33.0 36.5 24.0 39.0 80-81 31.977241750396225 35.0 33.0 36.0 24.5 38.0 82-83 31.894535821736117 35.0 33.0 36.0 25.0 37.0 84-85 31.70270780249943 35.0 33.0 35.0 24.0 37.0 86-87 31.521231698661513 35.0 33.0 35.0 24.0 36.5 88-89 31.35071546116337 35.0 33.0 35.0 23.5 36.0 90-91 31.217619219101017 35.0 33.0 35.0 23.0 36.0 92-93 31.099999424239314 35.0 33.0 35.0 23.0 36.0 94-95 30.994090863410722 35.0 33.0 35.0 20.5 35.5 96-97 30.908221653207825 35.0 33.0 35.0 20.0 35.0 98-99 30.82522365685501 35.0 33.0 35.0 20.0 35.0 100-101 30.748801894357335 35.0 32.5 35.0 19.0 35.0 102-103 29.656035071153553 33.5 30.0 35.0 16.0 35.0 104-105 30.458043795498178 35.0 32.0 35.0 18.0 35.0 106-107 30.538918020053224 35.0 32.0 35.0 16.5 35.0 108-109 30.496450697089166 35.0 32.0 35.0 14.0 35.0 110-111 30.41844114364915 35.0 32.0 35.0 8.5 35.0 112-113 30.32865466711611 35.0 32.0 35.0 4.5 35.0 114-115 30.213260187300186 35.0 32.0 35.0 2.0 35.0 116-117 30.104184157578374 35.0 32.0 35.0 2.0 35.0 118-119 29.952405789849443 35.0 32.0 35.0 2.0 35.0 120-121 29.79361858262375 35.0 32.0 35.0 2.0 35.0 122-123 29.599873803726325 35.0 32.0 35.0 2.0 35.0 124-125 28.748545419143312 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3408157257980342 1101 2 0.4136598549731758 1101 3 0.38660667564981566 1101 4 0.35517296966369116 1101 5 0.3851525015937156 1101 6 0.3713197958561807 1101 7 0.42451618220041354 1101 8 0.2646132337750515 1101 9 0.279545756261399 1101 10-11 0.3887576755014521 1101 12-13 0.4910220206320446 1101 14-15 0.5128467979712497 1101 16-17 0.45299012591942756 1101 18-19 0.4420200228031206 1101 20-21 0.4597549716807734 1101 22-23 0.5832020433478959 1101 24-25 0.4619389034381882 1101 26-27 0.5208173982419098 1101 28-29 0.5399233723842087 1101 30-31 0.6284209022995242 1101 32-33 0.6061974712662277 1101 34-35 0.5804363597748363 1101 36-37 0.5775427483242339 1101 38-39 0.51546138850631 1101 40-41 0.639144179236645 1101 42-43 0.6226906990953509 1101 44-45 0.6617783983446728 1101 46-47 0.6476343049937086 1101 48-49 0.5485348045862892 1101 50-51 0.5398275062252793 1101 52-53 0.5618295797690394 1101 54-55 0.562578988831703 1101 56-57 0.620939345749953 1101 58-59 0.5333913764301457 1101 60-61 0.5462341946807996 1101 62-63 0.5902615819533565 1101 64-65 0.695004201171102 1101 66-67 0.6747042370852299 1101 68-69 0.5459655364774179 1101 70-71 0.5509171418422341 1101 72-73 0.6062001028168424 1101 74-75 0.5577841350680188 1101 76-77 0.43682609334069866 1101 78-79 0.48972615246221096 1101 80-81 0.5061518265518856 1101 82-83 0.39710721064054866 1101 84-85 0.4188380641956648 1101 86-87 0.37490644384164895 1101 88-89 0.36174357816713254 1101 90-91 0.42298655424822584 1101 92-93 0.4288089752430366 1101 94-95 0.43844769327467503 1101 96-97 0.4736869573328022 1101 98-99 0.48102125276639995 1101 100-101 0.4045848602075637 1101 102-103 0.48427761375830514 1101 104-105 0.5013551866456964 1101 106-107 0.5245347156605824 1101 108-109 0.5454597530832501 1101 110-111 0.5394028418243622 1101 112-113 0.5775312333797373 1101 114-115 0.6474984376950914 1101 116-117 0.6047952761903659 1101 118-119 0.5810431237061238 1101 120-121 0.6369586248875478 1101 122-123 0.7099447407303998 1101 124-125 0.7189587490443579 1102 1 0.3995324216041247 1102 2 0.4198417493708497 1102 3 0.40436523301506 1102 4 0.47312331503322014 1102 5 0.387689963986773 1102 6 0.42356878780292107 1102 7 0.41696319082660693 1102 8 0.4284040002240488 1102 9 0.4741471768986827 1102 10-11 0.4946609220998255 1102 12-13 0.5057866089054457 1102 14-15 0.6140698653572372 1102 16-17 0.6629419456715553 1102 18-19 0.513856029933244 1102 20-21 0.5664979724698682 1102 22-23 0.5500272521366298 1102 24-25 0.5853502463287228 1102 26-27 0.6105657742199853 1102 28-29 0.5739029636175417 1102 30-31 0.6667718014166653 1102 32-33 0.5825628684789663 1102 34-35 0.6726004498856355 1102 36-37 0.5716807634136671 1102 38-39 0.6430402932713122 1102 40-41 0.5782609163044228 1102 42-43 0.6233548282996253 1102 44-45 0.6505186225112709 1102 46-47 0.7762898901376616 1102 48-49 0.6181158736187982 1102 50-51 0.6964069385879696 1102 52-53 0.6707537338223091 1102 54-55 0.5786325449255898 1102 56-57 0.5157401759771574 1102 58-59 0.5498097830851094 1102 60-61 0.7408934342436595 1102 62-63 0.5463395008399701 1102 64-65 0.512980367947165 1102 66-67 0.5517859319419145 1102 68-69 0.6311857707360531 1102 70-71 0.5633658193328657 1102 72-73 0.5710164324583076 1102 74-75 0.5928451373196708 1102 76-77 0.5632655710518293 1102 78-79 0.548619590211274 1102 80-81 0.545929013437771 1102 82-83 0.5290828504759446 1102 84-85 0.48629717865940236 1102 86-87 0.5065333961338077 1102 88-89 0.5030981047648204 1102 90-91 0.4899047332411648 1102 92-93 0.41737863357917604 1102 94-95 0.5101086667482733 1102 96-97 0.5086465793547141 1102 98-99 0.5129544482541597 1102 100-101 0.5130781975619385 1102 102-103 0.572057321023717 1102 104-105 0.5736411958454752 1102 106-107 0.570105621702858 1102 108-109 0.549585659049967 1102 110-111 0.5833979193312899 1102 112-113 0.7380047046769107 1102 114-115 0.7758476482367982 1102 116-117 0.7887129420403376 1102 118-119 0.8067557912413896 1102 120-121 0.7737725261341311 1102 122-123 0.7614011534734288 1102 124-125 0.9455998702584516 1103 1 0.212238303957065 1103 2 0.2590574356453601 1103 3 0.258832256187965 1103 4 0.29501279809917236 1103 5 0.262185507658792 1103 6 0.34114454537868255 1103 7 0.2744533869050372 1103 8 0.23633269897627684 1103 9 0.2871453943674851 1103 10-11 0.3374737563244281 1103 12-13 0.21506290124056449 1103 14-15 0.3553425926299667 1103 16-17 0.39046779237387597 1103 18-19 0.2578382046213008 1103 20-21 0.37730367656968866 1103 22-23 0.3198026532061533 1103 24-25 0.36221655648915174 1103 26-27 0.2887404622770262 1103 28-29 0.3639671347405411 1103 30-31 0.34762741638993333 1103 32-33 0.050812422846171046 1103 34-35 0.3112100755540794 1103 36-37 0.42167363328888996 1103 38-39 0.45633797598075887 1103 40-41 0.5407992407251001 1103 42-43 0.4986311206347409 1103 44-45 0.49020489702107994 1103 46-47 0.5007569133105676 1103 48-49 0.40095366328012005 1103 50-51 0.4213160294970635 1103 52-53 0.31035801189717915 1103 54-55 0.38130277665464263 1103 56-57 0.42627493533544936 1103 58-59 0.48422333032218035 1103 60-61 0.4074781044753877 1103 62-63 0.4220364705369377 1103 64-65 0.3640463216013572 1103 66-67 0.3102493900524266 1103 68-69 0.3296634890961201 1103 70-71 0.34058507066976773 1103 72-73 0.4412552916383419 1103 74-75 0.39370431735531497 1103 76-77 0.35114435893061113 1103 78-79 0.4448299288921902 1103 80-81 0.37765093857146326 1103 82-83 0.3744553994955524 1103 84-85 0.2886394246487036 1103 86-87 0.29614123927106206 1103 88-89 0.29761503881116624 1103 90-91 0.27245731791139605 1103 92-93 0.2813394178929016 1103 94-95 0.3424865633614651 1103 96-97 0.3317374884456257 1103 98-99 0.3200239669707372 1103 100-101 0.3655630460467876 1103 102-103 0.4025136490094532 1103 104-105 0.42262871812711467 1103 106-107 0.39671881243369356 1103 108-109 0.3685910066435518 1103 110-111 0.2998970280656934 1103 112-113 0.2339155781171982 1103 114-115 0.35925228281789856 1103 116-117 0.3178501969422953 1103 118-119 0.4167272707422818 1103 120-121 0.3745247542981289 1103 122-123 0.43509455810800546 1103 124-125 0.4742273212388426 1104 1 0.3560087737127411 1104 2 0.33848361003455096 1104 3 0.34680548619853013 1104 4 0.3812813305529801 1104 5 0.28513571419758676 1104 6 0.24685910556312507 1104 7 0.29951253064963623 1104 8 0.27265560396843114 1104 9 0.29765524098441176 1104 10-11 0.26506663473872294 1104 12-13 0.3285805931295087 1104 14-15 0.29813275561949837 1104 16-17 0.37550291997962404 1104 18-19 0.41225602139417106 1104 20-21 0.3584072021388707 1104 22-23 0.31602680383537773 1104 24-25 0.2764834878670328 1104 26-27 0.32432969023820846 1104 28-29 0.4522179913161537 1104 30-31 0.4889595115578871 1104 32-33 -0.25404946834471787 1104 34-35 0.24646448651824926 1104 36-37 0.3293429790892617 1104 38-39 0.40072492400982895 1104 40-41 0.3918166924526574 1104 42-43 0.408120057200108 1104 44-45 0.4409239081965879 1104 46-47 0.36987171045424816 1104 48-49 0.3796719059391833 1104 50-51 0.3848887983977676 1104 52-53 0.34498601791722194 1104 54-55 0.3989107051826153 1104 56-57 0.3345688288318769 1104 58-59 0.4480955644635287 1104 60-61 0.39697165213942753 1104 62-63 0.3645682839155313 1104 64-65 0.31065774047483785 1104 66-67 0.40154843901333237 1104 68-69 0.38426357235206865 1104 70-71 0.40692789304640087 1104 72-73 0.29096370369124 1104 74-75 0.39910000725893724 1104 76-77 0.4306217677493436 1104 78-79 0.3827790787208798 1104 80-81 0.39389724809145577 1104 82-83 0.34583437416653595 1104 84-85 0.38509637171712896 1104 86-87 0.3428372589963189 1104 88-89 0.37422857110486873 1104 90-91 0.3320439841511096 1104 92-93 0.298179598494265 1104 94-95 0.33244794541020184 1104 96-97 0.3511039106813705 1104 98-99 0.3400926851493544 1104 100-101 0.3193810997788944 1104 102-103 0.3595742657438983 1104 104-105 0.40900042391340463 1104 106-107 0.37032443541233917 1104 108-109 0.33322266311670035 1104 110-111 0.3501873351520537 1104 112-113 0.39968668830324106 1104 114-115 0.3837132005546202 1104 116-117 0.4045870761251109 1104 118-119 0.48665374933589334 1104 120-121 0.3757909491810878 1104 122-123 0.4628784128584016 1104 124-125 0.5908356020035193 1105 1 0.16270864346707725 1105 2 0.21056844173913092 1105 3 0.17028779654364 1105 4 0.2218962703041214 1105 5 0.1558054554264814 1105 6 0.2678315246101164 1105 7 0.1881504395104372 1105 8 0.16349499222338437 1105 9 0.2038967934472069 1105 10-11 0.23974859776205903 1105 12-13 0.16342765686797378 1105 14-15 0.34994226928709793 1105 16-17 0.32713897754984345 1105 18-19 0.26999539900010916 1105 20-21 0.24700620113131322 1105 22-23 0.24217996946035214 1105 24-25 0.35704841819789834 1105 26-27 0.36016055927988333 1105 28-29 0.35712022515217967 1105 30-31 0.3254305503284982 1105 32-33 0.07786092427633662 1105 34-35 0.4529356072043669 1105 36-37 0.4778916409993741 1105 38-39 0.5117931432485108 1105 40-41 0.529149072835537 1105 42-43 0.5017986371127918 1105 44-45 0.4551055312705472 1105 46-47 0.47392181817313883 1105 48-49 0.4626615354353234 1105 50-51 0.4845738332528171 1105 52-53 0.472911425651688 1105 54-55 0.41436918630518704 1105 56-57 0.3851967112339736 1105 58-59 0.4142306677180301 1105 60-61 0.4115915232044074 1105 62-63 0.43838132814171615 1105 64-65 0.5395266101226852 1105 66-67 0.39751614791836687 1105 68-69 0.3432095654547851 1105 70-71 0.32278939848968946 1105 72-73 0.22611426440292348 1105 74-75 0.33202144209104745 1105 76-77 0.41346558763351027 1105 78-79 0.3950203280962725 1105 80-81 0.34700164122038757 1105 82-83 0.3394971853430846 1105 84-85 0.1959135199104871 1105 86-87 0.2719004731971921 1105 88-89 0.30285456125862353 1105 90-91 0.34717787505603326 1105 92-93 0.3349842381888877 1105 94-95 0.323181253076676 1105 96-97 0.32544797221629906 1105 98-99 0.27701364487149505 1105 100-101 0.22206547111829877 1105 102-103 0.23618595344920124 1105 104-105 0.3338316779579813 1105 106-107 0.33057902882469037 1105 108-109 0.31826181873159953 1105 110-111 0.26147693106283043 1105 112-113 0.33745854540982023 1105 114-115 0.3209249603455717 1105 116-117 0.3930677072022135 1105 118-119 0.3935567695608384 1105 120-121 0.41029990664236493 1105 122-123 0.38793288667410764 1105 124-125 0.4524004340357557 1106 1 0.1259014952676374 1106 2 0.22589942624633608 1106 3 0.17573879504451995 1106 4 0.19838088499344764 1106 5 0.13985405205517054 1106 6 0.15717392510542538 1106 7 0.1505826079065642 1106 8 0.1562602455823523 1106 9 0.2752524560835852 1106 10-11 0.21864838518752094 1106 12-13 0.26333142696338285 1106 14-15 0.2888823517411083 1106 16-17 0.2662549760905293 1106 18-19 0.3545499782978112 1106 20-21 0.32179647168966596 1106 22-23 0.3751378746433929 1106 24-25 0.3665468916794694 1106 26-27 0.41019712406781395 1106 28-29 0.40950739181314333 1106 30-31 0.4515065362430377 1106 32-33 0.40352948736330063 1106 34-35 0.38474778057415193 1106 36-37 0.44490459404329386 1106 38-39 0.48063351611333616 1106 40-41 0.4405607171808654 1106 42-43 0.3860068636866174 1106 44-45 0.3155248683882945 1106 46-47 0.3525403623161978 1106 48-49 0.43168373704066454 1106 50-51 0.31311354591423424 1106 52-53 0.30520479537730694 1106 54-55 0.4064542503093733 1106 56-57 0.43524726735125796 1106 58-59 0.5056877727609503 1106 60-61 0.4706123362142165 1106 62-63 0.4279813213041095 1106 64-65 0.44335577353250955 1106 66-67 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78-79 0.40322766411708244 2205 80-81 0.43690827046280134 2205 82-83 0.46424062756349116 2205 84-85 0.353178771935422 2205 86-87 0.36246034353978374 2205 88-89 0.35435554393236046 2205 90-91 0.42429598449324146 2205 92-93 0.4057444054875319 2205 94-95 0.3535555881085841 2205 96-97 0.46890804636915817 2205 98-99 0.4579644211981062 2205 100-101 0.41275587001777936 2205 102-103 0.5767172243005056 2205 104-105 0.473380801592981 2205 106-107 0.40640645159649225 2205 108-109 0.3111065610681365 2205 110-111 0.3455568028605249 2205 112-113 0.40315827803449267 2205 114-115 0.409766946600584 2205 116-117 0.42089848245664285 2205 118-119 0.4389153234173868 2205 120-121 0.4409133286349167 2205 122-123 0.3807737997900489 2205 124-125 0.36048980612973835 2206 1 0.2764404071242623 2206 2 0.3390835696756014 2206 3 0.3724147339429251 2206 4 0.3647919033197553 2206 5 0.3110429527462131 2206 6 0.28420596034974466 2206 7 0.3957170294910597 2206 8 0.4233323878568669 2206 9 0.3990667433812405 2206 10-11 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-2.027104740478787 2216 88-89 -2.00492413493944 2216 90-91 -2.1309888892465416 2216 92-93 -2.225752357514537 2216 94-95 -2.227996732972933 2216 96-97 -2.199927247057108 2216 98-99 -2.2476415265589615 2216 100-101 -2.1344058748476833 2216 102-103 -2.2285407664976127 2216 104-105 -2.1405976612112347 2216 106-107 -2.28974066019612 2216 108-109 -2.382943889669189 2216 110-111 -2.2593386641753597 2216 112-113 -2.2451533481917565 2216 114-115 -2.3539270988486756 2216 116-117 -2.517613769704518 2216 118-119 -2.5397796327175683 2216 120-121 -2.6671362993593526 2216 122-123 -2.736158243884521 2216 124-125 -3.029675976178453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36838.0 3 7149.0 4 4407.0 5 3986.0 6 5118.0 7 5478.0 8 5603.0 9 6130.0 10 6602.0 11 6852.0 12 7108.0 13 7116.0 14 7194.0 15 6467.0 16 5692.0 17 5438.0 18 5158.0 19 5094.0 20 5246.0 21 5798.0 22 6971.0 23 9501.0 24 12533.0 25 14964.0 26 18543.0 27 21617.0 28 26039.0 29 32173.0 30 37920.0 31 45618.0 32 58979.0 33 77856.0 34 107751.0 35 161696.0 36 309108.0 37 565763.0 38 253885.0 39 1125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.31479932890495 18.111992931412612 12.062933568952912 24.510274170729527 2 15.997772338928428 20.475129184358412 38.71223730585534 24.814861170857817 3 18.831522596522966 25.474858278730768 28.9463720406811 26.74724708406517 4 12.694583195079332 16.102493935666796 36.25916485994322 34.94375800931065 5 13.868793938348528 38.499669739283355 33.55007131107194 14.08146501129618 6 32.72103008808579 35.70158175561192 17.496711253301903 14.080676903000388 7 29.173743751644306 32.440568271507495 20.87056037884767 17.515127598000525 8 26.763750332832725 34.90007651206039 19.882348236170735 18.453824918936157 9 27.472217500514418 15.752231204284586 19.047819527030477 37.72773176817052 10-11 25.382199256010534 26.40470447670066 27.480870628206596 20.73222563908221 12-13 25.578160461817735 24.05929745611239 27.535590291257538 22.826951790812338 14-15 22.738885566017704 25.697603401450586 24.712039515180667 26.851471517351044 16-17 23.38689348821275 28.97734538590308 24.97191130973024 22.66384981615393 18-19 23.121213269503635 27.81860113596652 26.51766579511341 22.54251979941644 20-21 24.031880230457215 27.18114111793959 26.085643054468594 22.7013355971346 22-23 23.722981684563297 27.039882979958108 26.140467738034488 23.096667597444107 24-25 23.58232419777934 27.29834482119774 26.01124961578462 23.1080813652383 26-27 23.23643502151137 27.68082799880725 26.062586801466146 23.02015017821524 28-29 23.118343796261552 27.537823630237014 26.360342932142444 22.983489641358993 30-31 23.121362489641886 27.303737920577575 26.259519004589542 23.315380585190994 32-33 23.014001937308773 27.519257959689135 26.17585195403194 23.29088814897015 34-35 23.52712548987927 27.444212378864002 25.915228551557284 23.113433579699443 36-37 23.5482076564676 27.235547302904017 25.693812308578444 23.522432732049936 38-39 23.068517642109693 27.515091760004207 26.076773413261815 23.339617184624284 40-41 23.145888748986778 27.235725010930857 26.47846575204163 23.139920488040737 42-43 23.34267683407836 27.38861425190639 25.86983960031554 23.39886931369971 44-45 23.062575273878583 27.51068954817265 25.98246545947964 23.444269718469123 46-47 23.13865764554656 27.55152220882799 25.879338310938323 23.430481834687132 48-49 23.340717885810427 27.47192918825243 25.850711754957352 23.33664117097979 50-51 23.175432396015108 27.428286372754133 25.87139249240815 23.524888738822604 52-53 23.495954649792612 27.341029320538418 25.8110094218385 23.352006607830468 54-55 23.134668412227587 27.084766895835084 26.029936597644436 23.750628094292896 56-57 23.118079997307976 27.73182699112578 25.90624497217014 23.243848039396102 58-59 23.314543665599658 27.113495798487257 25.911624930255257 23.66033560565783 60-61 23.233767493786285 27.360469742030496 26.026620713337095 23.379142050846124 62-63 23.055322823853395 27.527111270999104 25.879160440764913 23.538405464382585 64-65 22.93579911735423 27.313736865108563 25.894834613052236 23.85562940448497 66-67 23.057752801483076 27.63577682392598 25.656401377985944 23.650068996605004 68-69 22.82842065976738 27.345893793248198 26.14198651933161 23.683699027652807 70-71 22.832376555394273 27.441874655295617 26.086964526319306 23.638784262990804 72-73 23.046475199943185 27.393787787321745 25.807368009879777 23.752369002855296 74-75 22.780178478905245 27.58311788392296 25.866369640175375 23.77033399699642 76-77 23.069514393148584 27.360408774835353 25.947512894538967 23.6225639374771 78-79 22.939746334411513 27.63944334452021 25.96170159375471 23.45910872731357 80-81 22.87172443758734 28.00662904317321 25.89787580775354 23.223770711485912 82-83 23.078790531322337 27.62504251088976 25.74609406131089 23.55007289647701 84-85 23.019323283966717 27.32613081675717 25.722410553965354 23.93213534531076 86-87 23.10250786337194 27.115168155184566 26.031968946255564 23.75035503518793 88-89 22.941087359827304 27.18360118181909 26.025688837784966 23.84962262056864 90-91 22.940722317736288 27.462032063624463 26.06035951271874 23.53688610592051 92-93 23.08992752784761 27.09743244522515 26.076064204647054 23.73657582228019 94-95 22.987228806049817 27.017609365016344 26.082816867336824 23.912344961597018 96-97 23.273045450828754 26.98899137709141 25.99563609364389 23.742327078435945 98-99 22.914630896331172 27.033961248569593 25.963647602832797 24.087760252266442 100-101 22.859542733454614 26.98014012244025 26.13871164937957 24.021605494725566 102-103 23.160921186409723 26.67351133140642 26.247064366740187 23.91850311544367 104-105 22.85385714683664 27.007149648561896 26.163414381605065 23.9755788229964 106-107 22.97992174518384 27.11262118419735 26.11902311037272 23.788433960246092 108-109 23.002803332038464 27.351647594932448 25.714171571490223 23.93137750153886 110-111 22.746175570815332 27.480578490344516 25.9463902977496 23.826855641090557 112-113 22.877298286191394 27.34848510739203 26.07296732509312 23.701249281323452 114-115 22.506770216410345 27.721400928772223 25.83409959933357 23.937729255483863 116-117 22.641883381102684 27.644890431900816 26.014038511278542 23.69918767571796 118-119 22.734674042354456 27.821000724736006 25.58628408178893 23.858041151120606 120-121 22.635291395334466 27.992095703517883 25.76595551663886 23.60665738450879 122-123 22.776240646881224 27.91354939850371 25.72636663437027 23.583843320244792 124-125 22.855809674027338 28.085436382754995 25.515325972660357 23.543427970557307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9757.0 1 5513.0 2 1695.5 3 2163.5 4 1952.5 5 1506.5 6 1180.0 7 1051.0 8 1039.5 9 999.0 10 864.5 11 732.0 12 687.0 13 675.5 14 685.0 15 740.0 16 824.0 17 929.0 18 1071.5 19 1223.5 20 1526.5 21 2050.0 22 2780.5 23 3711.0 24 4992.5 25 6808.0 26 8611.5 27 10407.5 28 12356.5 29 14996.5 30 18729.0 31 22348.5 32 25975.0 33 28871.0 34 31601.0 35 34073.5 36 37006.5 37 41726.0 38 45660.0 39 47348.5 40 48587.0 41 52828.0 42 56826.5 43 60447.0 44 64253.0 45 66141.5 46 66274.5 47 66173.5 48 69415.0 49 71034.5 50 70581.5 51 70398.0 52 70992.0 53 72422.5 54 72350.0 55 68111.5 56 61635.5 57 56194.0 58 51297.0 59 46848.5 60 41676.0 61 35594.5 62 29711.0 63 23636.0 64 18689.0 65 15367.0 66 11902.5 67 10119.5 68 7877.0 69 4962.5 70 3642.0 71 2859.0 72 2402.5 73 2084.5 74 1628.0 75 1362.5 76 926.0 77 522.5 78 293.0 79 149.0 80 91.0 81 53.5 82 30.0 83 23.5 84 18.5 85 14.0 86 9.0 87 11.0 88 13.5 89 10.5 90 7.0 91 7.0 92 6.5 93 3.0 94 0.5 95 2.0 96 3.5 97 2.5 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47871883826149586 2 0.46950666730872703 3 0.46563336815813106 4 0.46181241088794855 5 0.4704488211561693 6 0.528443624654282 7 0.5242562742212051 8 0.5318981887615701 9 0.5395924451823486 10-11 0.5560539665723815 12-13 0.5655801888076311 14-15 0.5435965990339783 16-17 0.5383362400524256 18-19 0.5474960691247809 20-21 0.5478624622876751 22-23 0.5585140349518141 24-25 0.5516834195578577 26-27 0.5603983426466985 28-29 0.5399064964648294 30-31 0.5462136930546512 32-33 0.5473652144237473 34-35 0.5459519836525839 36-37 0.4942905476844999 38-39 0.4614460177250544 40-41 0.46005895789409773 42-43 0.47191439380774614 44-45 0.4776720006532267 46-47 0.48154529980382266 48-49 0.49596548785773054 50-51 0.4780907356965344 52-53 0.4781692485171545 54-55 0.46743916303239547 56-57 0.45024485531657416 58-59 0.46788406901590984 60-61 0.46461270149006867 62-63 0.4807601715976208 64-65 0.48086485535844764 66-67 0.48863762459984633 68-69 0.49711700922682667 70-71 0.49361010323912496 72-73 0.505805761375461 74-75 0.4946831117876008 76-77 0.4930866844349903 78-79 0.5039214536805763 80-81 0.49659359042269213 82-83 0.4989489750412977 84-85 0.4955467528144229 86-87 0.48657012032351465 88-89 0.4909144963978318 90-91 0.4767821886861979 92-93 0.4761017442408229 94-95 0.46955900918914056 96-97 0.4595355390899631 98-99 0.42307941938198895 100-101 0.4206716928829698 102-103 0.4091826501322156 104-105 0.4137625646683933 106-107 0.41274189800033084 108-109 0.42590588092431575 110-111 0.44833437668148296 112-113 0.4492241886485117 114-115 0.44150376128752655 116-117 0.4354844450399787 118-119 0.4780907356965344 120-121 0.4720452485087798 122-123 0.4366883082894883 124-125 0.44574345360101664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1910516.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.199851914442995 #Duplication Level Percentage of deduplicated Percentage of total 1 78.98576537630805 48.33917144378991 2 11.343691860889768 13.88464524099052 3 4.028939420275538 7.397114876793741 4 1.9370891913344872 4.74198286618955 5 1.0659561948716356 3.261818063671361 6 0.6546476749095486 2.4038604456359276 7 0.4104753468701076 1.7584721310086144 8 0.3049792096085306 1.4931745972020751 9 0.21317502676466296 1.1741652062869314 >10 0.9870915960270104 10.400196978112303 >50 0.05046893491183617 2.099329604771704 >100 0.016949725187128357 1.6150375511321955 >500 3.8522102084810334E-4 0.13485168514094123 >1k 1.9261051042405167E-4 0.35209963594055194 >5k 1.9261051042405167E-4 0.9440796733337398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 8009 0.419206120231393 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2987 0.15634519679500197 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.234188041345898E-5 0.0 0.0 0.0 0.0 6 5.234188041345898E-5 0.0 0.0 0.0 0.0 7 2.0936752165383592E-4 0.0 0.0 0.0 0.0 8 2.6170940206729493E-4 0.0 0.0 0.0 0.0 9 2.6170940206729493E-4 0.0 0.0 0.0 0.0 10-11 2.6170940206729493E-4 0.0 0.0 0.0 0.0 12-13 2.878803422740244E-4 0.0 0.0 5.234188041345898E-5 0.0 14-15 4.1873504330767184E-4 0.0 0.0 7.851282062018847E-5 0.0 16-17 5.757606845480488E-4 0.0 0.0 1.5702564124037694E-4 0.0 18-19 8.374700866153437E-4 0.0 0.0 1.5702564124037694E-4 0.0 20-21 9.944957278557208E-4 0.0 0.0 2.3553846186056542E-4 0.0 22-23 9.944957278557208E-4 0.0 0.0 4.972478639278604E-4 0.0 24-25 0.0010468376082691797 0.0 0.0 8.898119670288026E-4 0.0 26-27 0.0010468376082691797 0.0 0.0 0.0019104786350912528 0.0 28-29 0.001334717950543204 0.0 0.0 0.004710769237211308 0.0 30-31 0.0014655726515768516 0.0 0.0 0.012352683777576319 0.0 32-33 0.0014655726515768516 0.0 0.0 0.026929897472724645 0.0 34-35 0.0015440854721970399 0.0 0.0 0.04823304280100245 0.0 36-37 0.0016749401732306874 0.0 0.0 0.08327593173781325 0.0 38-39 0.0017796239340576053 0.0 0.0 0.1345448036027963 0.0 40-41 0.0018057948742643349 0.0 0.0 0.19316770966587038 0.0 42-43 0.001910478635091253 0.0 0.0 0.24982779521343973 0.0 44-45 0.0020413333361249003 0.0 0.0 0.32132680385822465 0.0 46-47 0.0024862393196393015 0.0 0.0 0.4119829407343356 0.0 48-49 0.002617094020672949 0.0 0.0 0.5106212143734992 0.0 50-51 0.002617094020672949 0.0 0.0 0.6199110606768015 0.0 52-53 0.002617094020672949 0.0 0.0 0.7293317616811374 0.0 54-55 0.002617094020672949 0.0 0.0 0.8465514028670789 0.0 56-57 0.002878803422740244 0.0 0.0 0.9828234885235194 0.0 58-59 0.002931145303153703 0.0 0.0 1.141916634040228 0.0 60-61 0.002931145303153703 0.0 0.0 1.326709642839945 0.0 62-63 0.002931145303153703 0.0 0.0 1.506661027701417 0.0 64-65 0.002983487183567162 0.0 0.0 1.68988378008873 0.0 66-67 0.003245196585634457 0.0 0.0 1.9027582077302676 0.0 68-69 0.003245196585634457 0.0 0.0 2.1415680371166745 0.0 70-71 0.0033498803464613747 0.0 0.0 2.4203408921987566 0.0 72-73 0.0034807350474950222 0.0 0.0 2.7044526190830123 0.0 74-75 0.0035592478681152106 0.0 0.0 2.989349474173469 0.0 76-77 0.0035592478681152106 0.0 0.0 3.305311235289315 0.0 78-79 0.0035592478681152106 0.0 0.0 3.662780107573033 0.0 80-81 0.0041088376124565305 0.0 0.0 4.060473714954494 0.0 82-83 0.004265863253696907 0.0 0.0 4.479732177066301 0.0 84-85 0.0043705470145238255 0.0 0.0 4.898336365672939 0.0 86-87 0.004449059835144014 0.0 0.0 5.322698161125057 0.0 88-89 0.004449059835144014 0.0 0.0 5.793827426726602 0.0 90-91 0.004710769237211308 0.0 0.0 6.319889495822071 0.0 92-93 0.004815452998038226 0.0 0.0 6.88332366753275 0.0 94-95 0.004815452998038226 0.0 0.0 7.449217907622861 0.0 96-97 0.0048677948784516855 0.0 0.0 8.007915139156124 0.0 98-99 0.004998649579485332 0.0 0.0 8.609899105791314 0.0 100-101 0.005260358981552627 0.0 0.0 9.280320081067105 0.0 102-103 0.005600581204240111 0.0 0.0 9.985443723057017 0.0 104-105 0.006124000008374701 0.0 0.0 10.688185809488118 0.0 106-107 0.006359538470235266 0.0 0.0 11.364966323234142 0.0 108-109 0.006490393171268914 0.0 0.0 12.074172631896305 0.0 110-111 0.006568905991889102 0.0 0.0 12.84969610304232 0.0 112-113 0.006595076932095832 0.0 0.0 13.674342428956365 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2445 0.0 54.52244 1 GTATCAA 4190 0.0 48.29154 1 GTACATG 10505 0.0 42.205242 1 TACATGG 10945 0.0 39.912533 2 ATCAACG 4940 0.0 38.43805 3 ACATGGG 11120 0.0 37.631203 3 TCAACGC 5040 0.0 37.5642 4 CAACGCA 5260 0.0 35.993076 5 AACGCAG 5315 0.0 35.620617 6 TATCAAC 5585 0.0 35.37887 2 CATGGGG 4905 0.0 33.13609 4 CATGGGA 6880 0.0 32.190826 4 ACGCAGA 6295 0.0 29.793074 7 CGCAGAG 6345 0.0 29.27679 8 GAGTACA 7500 0.0 26.820173 1 ATGGGGA 2885 0.0 26.620825 5 ATGGGAG 2635 0.0 26.20927 5 AGTACAT 7370 0.0 25.760378 2 GCAGAGT 6860 0.0 25.51665 9 ATGGGAT 3115 0.0 25.037495 5 >>END_MODULE