FastQCFastQC Report
Thu 26 May 2016
SRR1364078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910516
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93400.4888731630617069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79970.41857801766643155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.27840646191918833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.079.581961
GTATCAA74850.057.7351571
ATCAACG99450.043.0727653
TCAACGC100800.042.49594
CAACGCA103550.041.3098685
TATCAAC104300.041.2991072
AACGCAG105700.040.6947486
GTACATG86800.036.601731
ACGCAGA122300.034.929777
GTGGTAT15200.034.509071
CGCAGAG124650.034.2712488
TACATGG93150.033.532262
ACATGGG89600.033.1335223
CATGGGG43200.030.8488734
GCAGAGT137550.030.797629
TGGTATC17550.028.4766272
AGAGTAC130450.027.2743810-11
CAGAGTA133650.026.46553610-11
ATGGGGA27550.026.3459135
GAGTACT96850.026.2623912-13
Produced by FastQC (version 0.11.5)