FastQCFastQC Report
Thu 26 May 2016
SRR1364077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727653
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69650.40314808587141054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.053.95991
GTATCAA26850.049.8634531
ATCAACG31500.041.571463
TCAACGC31450.041.451914
CAACGCA32150.040.3642235
AACGCAG33400.039.032956
TATCAAC34550.038.2372782
GTACATG62900.033.3939821
TACATGG65050.031.5612262
ACGCAGA41900.030.5498267
ACATGGG64350.029.8770123
CGCAGAG42700.029.6986038
GTGGTAT10100.024.7441221
CATGGGG42200.024.685774
GCAGAGT51600.023.0762149
GAGTACA48950.022.8533171
GTGTAGC11350.022.543261
AGAGTAC43450.021.44446410-11
GTATAGT5700.020.87851
AGTACAT49450.020.6987532
Produced by FastQC (version 0.11.5)