FastQCFastQC Report
Thu 26 May 2016
SRR1364077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727653
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.23164373864427637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.22973363285335655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.13388105134538011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28200.073.356181
GTATCAA50300.050.4891361
ATCAACG64900.039.1434173
TCAACGC65300.038.7225464
TATCAAC67350.037.808922
CAACGCA67100.037.6837845
AACGCAG69500.036.469146
GTGGTAT14400.034.361481
TGGTATC14500.033.2357252
GTACATG59550.031.7346551
ACGCAGA82350.030.7811187
TACATGG61450.030.304742
CGCAGAG83500.030.2146668
ACATGGG61150.029.7714863
GCAGAGT95500.025.8572989
GAGTACT62900.025.06771712-13
AGAGTAC84100.024.55079710-11
CAGAGTA89600.024.43835410-11
GTACTTT65800.023.77964614-15
CATGGGG42250.023.5167834
Produced by FastQC (version 0.11.5)