##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1727653 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06337152194335 33.0 31.0 34.0 30.0 34.0 2 32.11107033646224 33.0 31.0 34.0 30.0 34.0 3 32.24315646718409 34.0 31.0 34.0 30.0 34.0 4 35.87509181531245 37.0 35.0 37.0 35.0 37.0 5 35.809262623918116 37.0 35.0 37.0 35.0 37.0 6 35.842226419309895 37.0 35.0 37.0 35.0 37.0 7 35.77148651957309 37.0 35.0 37.0 33.0 37.0 8 35.74647628893071 37.0 35.0 37.0 33.0 37.0 9 37.406464724108375 39.0 37.0 39.0 34.0 39.0 10-11 37.32728823438503 39.0 37.0 39.0 34.0 39.0 12-13 37.26850328161963 39.0 37.0 39.0 33.0 39.0 14-15 38.58757661405386 40.0 38.0 41.0 34.0 41.0 16-17 38.558456183041386 40.0 38.0 41.0 34.0 41.0 18-19 38.68223769472226 40.0 38.0 41.0 34.0 41.0 20-21 38.7294673756825 40.0 38.0 41.0 34.0 41.0 22-23 38.705028150907616 40.0 38.0 41.0 34.0 41.0 24-25 38.67251728211626 40.0 38.0 41.0 34.0 41.0 26-27 38.55621875457629 40.0 38.0 41.0 34.0 41.0 28-29 38.482480856977645 40.0 38.0 41.0 34.0 41.0 30-31 38.35977016217956 40.0 38.0 41.0 34.0 41.0 32-33 38.234881367959886 40.0 38.0 41.0 33.5 41.0 34-35 38.1306541301986 40.0 37.5 41.0 33.0 41.0 36-37 38.028852437381815 40.0 37.0 41.0 33.0 41.0 38-39 37.923577246125234 40.0 37.0 41.0 33.0 41.0 40-41 37.793346522710294 40.0 37.0 41.0 33.0 41.0 42-43 37.65756636315278 40.0 37.0 41.0 33.0 41.0 44-45 37.49864208842864 40.0 37.0 41.0 32.0 41.0 46-47 37.269375852674116 40.0 36.5 41.0 31.5 41.0 48-49 37.0289256581038 40.0 36.0 41.0 31.0 41.0 50-51 36.8486414806677 40.0 36.0 41.0 31.0 41.0 52-53 36.684888400622114 39.5 35.5 41.0 31.0 41.0 54-55 36.50140016542674 39.0 35.0 41.0 30.0 41.0 56-57 36.29572026327046 39.0 35.0 41.0 30.0 41.0 58-59 36.065988945696844 39.0 35.0 41.0 30.0 41.0 60-61 35.80901228429552 38.0 35.0 40.0 29.0 41.0 62-63 35.543443040934726 37.5 35.0 40.0 29.0 41.0 64-65 35.32110296454207 37.0 34.5 40.0 29.0 41.0 66-67 35.110462286118796 37.0 34.0 40.0 29.0 41.0 68-69 34.79917784416199 36.0 34.0 39.0 29.0 41.0 70-71 34.48129109259787 36.0 34.0 39.0 29.0 41.0 72-73 34.15614941194789 35.0 34.0 38.5 29.0 40.0 74-75 33.9002722190162 35.0 34.0 37.0 28.5 39.5 76-77 33.72269865534341 35.0 34.0 37.0 29.0 39.0 78-79 33.454309111841326 35.0 34.0 36.5 29.0 39.0 80-81 33.20916468758483 35.0 34.0 36.0 29.0 38.0 82-83 32.97257637963179 35.0 34.0 36.0 29.0 37.0 84-85 32.780741850359995 35.0 33.5 35.0 29.0 37.0 86-87 32.616909182573124 35.0 33.0 35.0 28.0 36.0 88-89 32.48874802984164 35.0 33.0 35.0 28.0 36.0 90-91 32.3726046260447 35.0 33.0 35.0 27.0 36.0 92-93 32.28117596531248 35.0 33.0 35.0 27.0 36.0 94-95 32.20549699505629 35.0 33.0 35.0 27.0 35.0 96-97 32.146584412494875 35.0 33.0 35.0 27.0 35.0 98-99 32.092328436323726 35.0 33.0 35.0 27.0 35.0 100-101 32.03802412868788 35.0 33.0 35.0 27.0 35.0 102-103 30.82774029275555 34.0 31.0 34.5 24.5 35.0 104-105 31.787479025012544 35.0 33.0 35.0 26.0 35.0 106-107 31.945460691469876 35.0 33.0 35.0 27.0 35.0 108-109 31.950472982711226 35.0 33.0 35.0 27.0 35.0 110-111 31.914550838623263 35.0 33.0 35.0 27.0 35.0 112-113 31.86158013212144 35.0 33.0 35.0 27.0 35.0 114-115 31.797758577677346 35.0 33.0 35.0 26.0 35.0 116-117 31.74067043555621 35.0 33.0 35.0 25.0 35.0 118-119 31.658435750697624 35.0 33.0 35.0 25.0 35.0 120-121 31.546895701856798 35.0 33.0 35.0 25.0 35.0 122-123 31.41282595521207 35.0 33.0 35.0 25.0 35.0 124-125 30.560830502421496 34.5 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.283936204524899 1101 2 0.33305550088338265 1101 3 0.27192229939386436 1101 4 0.2101086327539079 1101 5 0.2365166298226029 1101 6 0.23113361191760617 1101 7 0.27942824314784076 1101 8 0.25874401210014497 1101 9 0.2927866015904428 1101 10-11 0.3626386659335026 1101 12-13 0.3480725082321925 1101 14-15 0.4049034474370927 1101 16-17 0.49275298242990573 1101 18-19 0.43201945960774424 1101 20-21 0.3840773828415749 1101 22-23 0.3653531216805064 1101 24-25 0.3315819018342836 1101 26-27 0.3837298123943569 1101 28-29 0.3914783425063675 1101 30-31 0.452300623497635 1101 32-33 0.40751649614683316 1101 34-35 0.4818708924174899 1101 36-37 0.542231660981102 1101 38-39 0.5135467570851802 1101 40-41 0.48250588878568124 1101 42-43 0.5003999343891934 1101 44-45 0.5493047913968994 1101 46-47 0.457816170173885 1101 48-49 0.46420928139932727 1101 50-51 0.3767941797595711 1101 52-53 0.3288917112274703 1101 54-55 0.3758887861751603 1101 56-57 0.40452460960347736 1101 58-59 0.31518485951130515 1101 60-61 0.4070538787456428 1101 62-63 0.4111204822739154 1101 64-65 0.3080733824804156 1101 66-67 0.3613533794785013 1101 68-69 0.40384761781788114 1101 70-71 0.4352931311816661 1101 72-73 0.36353077084882557 1101 74-75 0.37815307406560095 1101 76-77 0.3659277047455163 1101 78-79 0.33614744795870166 1101 80-81 0.3038513129362812 1101 82-83 0.43562452646254 1101 84-85 0.39557583633049376 1101 86-87 0.4271756271056546 1101 88-89 0.36325099371829594 1101 90-91 0.4082920707157527 1101 92-93 0.426932552338954 1101 94-95 0.3903583358234499 1101 96-97 0.3221795558370033 1101 98-99 0.378346408756876 1101 100-101 0.39780343814342345 1101 102-103 0.4849422611838001 1101 104-105 0.41624837623730215 1101 106-107 0.403097855169424 1101 108-109 0.43695287912266423 1101 110-111 0.4371836594160037 1101 112-113 0.48811497278591887 1101 114-115 0.5237752764490153 1101 116-117 0.40219627776134104 1101 118-119 0.4265928519377695 1101 120-121 0.45615206081620485 1101 122-123 0.5088236481835793 1101 124-125 0.5887518518173565 1102 1 0.3113535433851098 1102 2 0.3680365725400918 1102 3 0.2927264705425898 1102 4 0.2659327277468151 1102 5 0.3064374321963257 1102 6 0.30071248738874345 1102 7 0.32535346443939517 1102 8 0.3228441760812615 1102 9 0.43006230279637947 1102 10-11 0.3818644151170574 1102 12-13 0.41797125749593533 1102 14-15 0.49316573150327514 1102 16-17 0.5781491917837442 1102 18-19 0.4539759921675284 1102 20-21 0.46486241825098773 1102 22-23 0.45699450907690675 1102 24-25 0.45561018235537176 1102 26-27 0.5161687619197082 1102 28-29 0.4874799546647566 1102 30-31 0.5614006077355356 1102 32-33 0.5709753916385978 1102 34-35 0.5465870225737035 1102 36-37 0.6229694100982925 1102 38-39 0.6449635898991062 1102 40-41 0.7124240088375586 1102 42-43 0.6630372651194776 1102 44-45 0.6872598528276015 1102 46-47 0.7570758355196645 1102 48-49 0.8414331889062296 1102 50-51 0.7923345708255027 1102 52-53 0.7964313307802158 1102 54-55 0.8283686203689555 1102 56-57 0.8549006185851056 1102 58-59 0.7945943753937001 1102 60-61 0.8351960666906777 1102 62-63 0.8435426327217499 1102 64-65 0.8120857349872779 1102 66-67 0.808651009356808 1102 68-69 0.8140126497430415 1102 70-71 0.7817507380528355 1102 72-73 0.7544085261049247 1102 74-75 0.7189086039347785 1102 76-77 0.6952784584226492 1102 78-79 0.5963056358152556 1102 80-81 0.6096445750544319 1102 82-83 0.6043165074263541 1102 84-85 0.6526033812870367 1102 86-87 0.6310327091266359 1102 88-89 0.5915294408475233 1102 90-91 0.6454557089396715 1102 92-93 0.6241645759433823 1102 94-95 0.6205227732133594 1102 96-97 0.568459700848301 1102 98-99 0.649995373021163 1102 100-101 0.6551768505269031 1102 102-103 0.7806618147569537 1102 104-105 0.644757516871163 1102 106-107 0.5983737886589005 1102 108-109 0.6917048749636834 1102 110-111 0.731781200528804 1102 112-113 0.7212361169482726 1102 114-115 0.7359637330631514 1102 116-117 0.7086439434500207 1102 118-119 0.7573973825729325 1102 120-121 0.8655562258813099 1102 122-123 0.9680918305925275 1102 124-125 0.9659405020011071 1103 1 0.2300868147615418 1103 2 0.2954577884720422 1103 3 0.19195946738466318 1103 4 0.13266023129700955 1103 5 0.11993001455923746 1103 6 0.09671275098340715 1103 7 0.14525019888704804 1103 8 0.20272471456451768 1103 9 0.3081958754833849 1103 10-11 0.16967742075256353 1103 12-13 0.22532601735911584 1103 14-15 0.2659225852249065 1103 16-17 0.2616889555070756 1103 18-19 0.23739390847778452 1103 20-21 0.3596940093937846 1103 22-23 0.39695789494511047 1103 24-25 0.35854626742247575 1103 26-27 0.45797926392688026 1103 28-29 0.45458245548001486 1103 30-31 0.42738662106945924 1103 32-33 0.42421919481986237 1103 34-35 0.4596608879445512 1103 36-37 0.48256904835889713 1103 38-39 0.4957295511809008 1103 40-41 0.5462042636179376 1103 42-43 0.575438273863945 1103 44-45 0.5123987870091682 1103 46-47 0.5190568249269063 1103 48-49 0.5326641473176608 1103 50-51 0.5565104676464259 1103 52-53 0.622881466067831 1103 54-55 0.6587217538635031 1103 56-57 0.5814666104980617 1103 58-59 0.49784663079503844 1103 60-61 0.4759395312622061 1103 62-63 0.5100933038462614 1103 64-65 0.5905983031389965 1103 66-67 0.4557480950209083 1103 68-69 0.44125789279711825 1103 70-71 0.4727534604751469 1103 72-73 0.4185923802547791 1103 74-75 0.3500065746726406 1103 76-77 0.27494466996309797 1103 78-79 0.24075563982647452 1103 80-81 0.21352750412246024 1103 82-83 0.2510351315872512 1103 84-85 0.22158411126712707 1103 86-87 0.13067729913274917 1103 88-89 0.13006817832287254 1103 90-91 0.05880619242228846 1103 92-93 0.08290445802384738 1103 94-95 0.11367725635424364 1103 96-97 0.04965325069738924 1103 98-99 0.1102246170254162 1103 100-101 0.1291763108350672 1103 102-103 0.1573841662296971 1103 104-105 0.1932888882822894 1103 106-107 0.15937674289866166 1103 108-109 0.18443205865790446 1103 110-111 0.1710312919534367 1103 112-113 0.2222038245186546 1103 114-115 0.26023052576210404 1103 116-117 0.16945702878904711 1103 118-119 0.13714605860884177 1103 120-121 0.10083725112910003 1103 122-123 0.13482313699058324 1103 124-125 0.29849433834529293 1104 1 0.16480215773448492 1104 2 0.18276927553056765 1104 3 0.12325329589138789 1104 4 0.16712483413656543 1104 5 0.13954511903603617 1104 6 0.10441063528504202 1104 7 0.11626007151817674 1104 8 0.1350756335438561 1104 9 0.1750684051018041 1104 10-11 0.16624210563730912 1104 12-13 0.10561303946422385 1104 14-15 0.16703177805306524 1104 16-17 0.2818405193364484 1104 18-19 0.1484507530241359 1104 20-21 0.22624277971017648 1104 22-23 0.30379036736212583 1104 24-25 0.25130318972338017 1104 26-27 0.2491025164115186 1104 28-29 0.2233194200802444 1104 30-31 0.2589394962613625 1104 32-33 0.22195178088620793 1104 34-35 0.22338703963814766 1104 36-37 0.16706632799589016 1104 38-39 0.24614304740462245 1104 40-41 0.27447602469064236 1104 42-43 0.35628776071526147 1104 44-45 0.3744172817102367 1104 46-47 0.41713877017221535 1104 48-49 0.32476587235954923 1104 50-51 0.3280764998931147 1104 52-53 0.30276854405575193 1104 54-55 0.3811717340324279 1104 56-57 0.21608285042162834 1104 58-59 0.36320023874253593 1104 60-61 0.39601892619989343 1104 62-63 0.3926813062029666 1104 64-65 0.32800131924141596 1104 66-67 0.25431454115624774 1104 68-69 0.2815796742241332 1104 70-71 0.21027924348865668 1104 72-73 0.3566712226432571 1104 74-75 0.3219126392141547 1104 76-77 0.2656366881386134 1104 78-79 0.314774080109828 1104 80-81 0.24199497278780058 1104 82-83 0.28435414974497775 1104 84-85 0.2584537551763688 1104 86-87 0.23180335360698479 1104 88-89 0.24958703144838523 1104 90-91 0.23988594735414637 1104 92-93 0.26226763459575864 1104 94-95 0.278597890195158 1104 96-97 0.34755556512509145 1104 98-99 0.3697299285093365 1104 100-101 0.3451309599401604 1104 102-103 0.24319856411326768 1104 104-105 0.37084110575226603 1104 106-107 0.35397472410570785 1104 108-109 0.3302678992181569 1104 110-111 0.3694215083790091 1104 112-113 0.2976999807435696 1104 114-115 0.3464960282731653 1104 116-117 0.3767811051789707 1104 118-119 0.3716130029803182 1104 120-121 0.3701977957668099 1104 122-123 0.43215962480781656 1104 124-125 0.5424191749089786 1105 1 0.18693430021305346 1105 2 0.24720310333507456 1105 3 0.15614027981067125 1105 4 0.19591911856439737 1105 5 0.19670540141488857 1105 6 0.17432829897194324 1105 7 0.16776749756896692 1105 8 0.23968809509101163 1105 9 0.26182592267654314 1105 10-11 0.1953161790203808 1105 12-13 0.20733183781316455 1105 14-15 0.19444231987070282 1105 16-17 0.22979065236231122 1105 18-19 0.3514814844791232 1105 20-21 0.3578230545404608 1105 22-23 0.3899572807875913 1105 24-25 0.4026822818527904 1105 26-27 0.4224967634420196 1105 28-29 0.35888542643392185 1105 30-31 0.3760887695080015 1105 32-33 0.3976645655346758 1105 34-35 0.49294853039571507 1105 36-37 0.47491592495191526 1105 38-39 0.5278688276938013 1105 40-41 0.47897348876205115 1105 42-43 0.5927023796156163 1105 44-45 0.5582916946820546 1105 46-47 0.43204642876086297 1105 48-49 0.5175945684103453 1105 50-51 0.561060611833021 1105 52-53 0.6833599525378133 1105 54-55 0.632401209208048 1105 56-57 0.5751317531638378 1105 58-59 0.5487077098470223 1105 60-61 0.5672943367931182 1105 62-63 0.6560767984290621 1105 64-65 0.6032018461761268 1105 66-67 0.6597207907114182 1105 68-69 0.6549024434341106 1105 70-71 0.6871699472397665 1105 72-73 0.556533338997589 1105 74-75 0.5910331980568344 1105 76-77 0.47496256743535525 1105 78-79 0.42237894983347957 1105 80-81 0.5331554937024521 1105 82-83 0.4540685742925561 1105 84-85 0.46924823280990324 1105 86-87 0.4549247839483783 1105 88-89 0.5138591358469071 1105 90-91 0.4608517105560921 1105 92-93 0.5187234071415077 1105 94-95 0.5148621458535771 1105 96-97 0.4448961396646922 1105 98-99 0.5203279057091308 1105 100-101 0.5538071539424614 1105 102-103 0.5319712393531262 1105 104-105 0.507938108424117 1105 106-107 0.5649512822275291 1105 108-109 0.4934926572998748 1105 110-111 0.5661198466855275 1105 112-113 0.45182700257981523 1105 114-115 0.49387315020186406 1105 116-117 0.49514399497805783 1105 118-119 0.5228406262557215 1105 120-121 0.548527218688438 1105 122-123 0.5783814512209133 1105 124-125 0.6482037925558473 1106 1 0.2069512956034032 1106 2 0.22536555343895515 1106 3 0.1485649074114761 1106 4 0.15403011748408346 1106 5 0.1345505108561511 1106 6 0.10769247444270036 1106 7 0.22911113626607005 1106 8 0.1610479280112287 1106 9 0.19491669282376733 1106 10-11 0.22695675242641755 1106 12-13 0.27075493969460496 1106 14-15 0.3056410411405537 1106 16-17 0.22381362261140936 1106 18-19 0.24161085197859222 1106 20-21 0.2595934380488458 1106 22-23 0.2795724025671049 1106 24-25 0.3271054589487079 1106 26-27 0.2515512754492306 1106 28-29 0.3443387085093619 1106 30-31 0.3547181236313435 1106 32-33 0.33614661693739123 1106 34-35 0.3559807270940354 1106 36-37 0.33299615176628805 1106 38-39 0.3267573622202562 1106 40-41 0.3383690585033108 1106 42-43 0.3629685142063863 1106 44-45 0.44317173599538506 1106 46-47 0.4520071183417542 1106 48-49 0.375138279296543 1106 50-51 0.32079342816137313 1106 52-53 0.4834846236845749 1106 54-55 0.39959741660560155 1106 56-57 0.38986175910395815 1106 58-59 0.40704824598197575 1106 60-61 0.36911217405336316 1106 62-63 0.4798487190020211 1106 64-65 0.435198406759568 1106 66-67 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-1.5060923244896145 2216 94-95 -1.4919472415528219 2216 96-97 -1.462754070959889 2216 98-99 -1.5730681169652314 2216 100-101 -1.5360802901952368 2216 102-103 -1.7538093180531753 2216 104-105 -1.648361059002216 2216 106-107 -1.6063553034855182 2216 108-109 -1.7327916555409075 2216 110-111 -1.6746036024314428 2216 112-113 -1.716852066864245 2216 114-115 -1.8684973224772818 2216 116-117 -1.8391989170142367 2216 118-119 -1.834937659463506 2216 120-121 -1.9702824655575029 2216 122-123 -2.2063731365080486 2216 124-125 -2.4903736219537365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 3.0 4 22.0 5 84.0 6 271.0 7 610.0 8 1204.0 9 2017.0 10 3001.0 11 4178.0 12 5680.0 13 7750.0 14 10886.0 15 10101.0 16 5025.0 17 4272.0 18 4042.0 19 3846.0 20 3788.0 21 4141.0 22 4528.0 23 5387.0 24 7059.0 25 9682.0 26 12769.0 27 16871.0 28 21773.0 29 28312.0 30 35850.0 31 45490.0 32 57610.0 33 74631.0 34 100421.0 35 150602.0 36 296625.0 37 558215.0 38 229794.0 39 1104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09952757220353 18.671566433274503 13.888959754346821 24.33994624017515 2 16.756819645998338 19.742142220014948 38.157834568848806 25.343203565137916 3 19.875461102432027 23.014633146818255 29.45718845161615 27.652717299133567 4 13.608114592455777 16.023125014108736 35.33892511980126 35.02983527363423 5 13.859496090939558 37.2318399586028 34.21705631860101 14.691607631856629 6 34.22568073565699 35.58741251860182 15.85816133216566 14.328745413575527 7 29.27601781144709 31.925160897471887 19.939536469418336 18.859284821662683 8 26.882713137418218 35.24203066240732 18.612070826722725 19.26318537345173 9 26.121680684720832 15.35979736671658 19.234475904594266 39.28404604396832 10-11 25.807465486947972 25.75108811511171 26.931395279176517 21.510051118763794 12-13 26.2544857588823 22.92135598930393 27.54318904136232 23.280969210451445 14-15 22.992117051282868 24.71642164254049 25.172415988627346 27.1190453175493 16-17 22.882425038744994 27.752587702606295 26.074665331513895 23.290321927134812 18-19 22.809181010307046 26.135543422203416 28.336101057330374 22.719174510159156 20-21 23.345197212634712 25.39236756455145 28.634077560713873 22.628357662099972 22-23 23.042300739789763 25.335816856741488 28.523059896865867 23.09882250660289 24-25 22.98983071253313 25.530994939377294 28.536777929364288 22.942396418725288 26-27 22.845241492359865 25.750801810317235 28.412215878998847 22.99174081832405 28-29 22.77109465847598 25.861182772234937 28.46072098968948 22.907001579599605 30-31 22.92491605663869 25.573248794752185 28.367559920886894 23.134275227722235 32-33 22.7539037063577 25.702412463613932 28.3690069707285 23.17467685929987 34-35 23.133609584795096 25.77398354877976 28.20398540679176 22.88842145963339 36-37 23.40608906996949 25.407098531938992 28.153512308316543 23.033300089774972 38-39 23.067508348030536 25.689273251052153 28.105875427530876 23.13734297338644 40-41 23.17603708615098 25.64502246689584 28.13218279365127 23.04675765330191 42-43 23.236465887536443 25.612261258481883 27.88800760337869 23.263265250602984 44-45 23.241385856997905 25.77820893431725 27.86329199208406 23.11711321660079 46-47 23.52712031872141 25.958829695546505 27.315728332020377 23.198321653711712 48-49 23.56662477939725 26.041572005489527 27.09149348856512 23.300309726548097 50-51 23.408143880744568 26.321720854824438 26.96837848804129 23.301756776389702 52-53 23.70111359167611 26.06455115697423 26.92872932238129 23.305605928968376 54-55 23.436129824681228 26.120899277806366 27.00562555096423 23.437345346548177 56-57 23.33602291663893 26.382149656209897 26.849344168070786 23.432483259080385 58-59 23.36099899690505 26.038735787799983 27.024900254854415 23.575364960440552 60-61 23.286215461090855 26.12298302957828 27.17548026137193 23.415321247958936 62-63 23.211894981225974 26.116413423297384 27.284790406406845 23.386901189069796 64-65 23.097925335701092 26.120696690828538 27.185435964282178 23.595942009188185 66-67 23.071473264596538 26.27162398930804 27.019690875424633 23.63721187067079 68-69 23.15363675460292 26.061599175297356 27.190153346765815 23.59461072333391 70-71 23.177513076989417 25.949597517557056 27.04813987531061 23.824749530142917 72-73 23.255190712486826 26.09667566345788 26.896691638888132 23.75144198516716 74-75 23.072312553504666 26.373496298157097 26.767846320991538 23.786344827346696 76-77 23.318195262590347 26.15852257368812 26.8419642138786 23.681317949842935 78-79 23.25840316313519 26.221585005785304 26.760611071783515 23.759400759295993 80-81 23.34994353611518 26.26282592627107 26.613735512860508 23.773495024753235 82-83 23.24841851922811 26.333702427512932 26.738239681232283 23.679639372026674 84-85 23.29492670113732 26.277991008611107 26.60725272957012 23.81982956068146 86-87 23.33492315875931 26.108425708171723 26.659606992839418 23.897044140229546 88-89 23.18995770562723 26.205349106562487 26.5488497979629 24.055843389847382 90-91 23.16810725301898 26.346957404062042 26.599728070393763 23.88520727252521 92-93 23.177513076989417 26.04377152124877 26.668868111825695 24.109847289936116 94-95 23.056873505548896 25.946420581590736 26.77408095779158 24.22262495506879 96-97 23.211436939961562 25.761854870453128 26.75018523235083 24.27652295723448 98-99 23.01056478659154 25.875363070479374 26.552072034109237 24.56200010881985 100-101 22.901934067028133 25.741793687554377 26.835708203794507 24.52056404162299 102-103 22.887433801869552 25.51828310022291 26.874834667473962 24.719448430433573 104-105 22.73687958042444 25.755270744329827 26.670139974297523 24.837709700948214 106-107 22.903355875263802 25.65247180184251 26.627130727662546 24.81704159523114 108-109 22.86888173934289 25.620430898775897 26.512438724035693 24.998248637845517 110-111 22.831794880701175 25.697890163455167 26.59971575531801 24.87059920052565 112-113 22.826483731429565 25.729291474295863 26.623289215781348 24.820935578493224 114-115 22.708788856295648 25.896930924746474 26.445165143327436 24.94911507563044 116-117 22.60239948216947 25.74909999154704 26.605077796163286 25.043422730120206 118-119 22.494816612427915 25.902320461435103 26.416216108257906 25.186646817879076 120-121 22.52421816539708 25.95026537580311 26.437740535267405 25.0877759235324 122-123 22.44151706128063 25.96390945843347 26.325144848734126 25.269428631551772 124-125 22.428050005354823 25.945994448352018 26.25084302266682 25.375112523626342 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 168.0 1 121.0 2 108.5 3 172.5 4 146.5 5 101.5 6 232.5 7 573.0 8 940.0 9 1117.5 10 951.0 11 698.5 12 676.5 13 793.0 14 940.0 15 1102.5 16 1257.5 17 1431.5 18 1602.0 19 1800.5 20 2080.5 21 2388.5 22 2799.5 23 3440.5 24 4344.0 25 5487.0 26 7158.5 27 9271.5 28 11497.5 29 13877.5 30 16556.0 31 19962.0 32 24320.0 33 26895.0 34 28089.5 35 30534.5 36 33852.0 37 36960.0 38 39408.5 39 41541.5 40 43620.0 41 46312.0 42 49195.0 43 52114.5 44 54906.5 45 58505.0 46 60835.5 47 61614.0 48 63169.5 49 64321.0 50 64386.0 51 64645.5 52 65973.0 53 67434.5 54 68350.5 55 65978.0 56 61495.5 57 56786.5 58 52454.0 59 47143.5 60 39607.5 61 33296.0 62 27700.5 63 21214.0 64 15593.5 65 11605.0 66 8837.0 67 7135.5 68 5271.5 69 3584.5 70 2609.5 71 1894.5 72 1388.0 73 1067.5 74 854.0 75 631.5 76 397.0 77 197.0 78 93.0 79 53.0 80 32.5 81 16.0 82 7.0 83 4.5 84 4.0 85 2.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1704624713411779 2 0.001967987784584057 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.498789398102513E-4 12-13 0.00593290435058429 14-15 0.0 16-17 6.077609334744882E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.945839239708437E-4 96-97 0.0053830254107740385 98-99 0.0015628138289343983 100-101 0.002894099683211849 102-103 0.004283267531153536 104-105 0.011171224777197737 106-107 0.016178017229154233 108-109 0.02465772930096495 110-111 0.01551237430201551 112-113 0.023818440392833515 114-115 0.027291360012687733 116-117 0.02587325116791393 118-119 0.015020377355869495 120-121 0.022313508557563352 122-123 0.01432579343189865 124-125 0.013775914492088398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1727653.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.00945496316149 #Duplication Level Percentage of deduplicated Percentage of total 1 87.49161540749077 68.25173231784898 2 8.485024619370325 13.238242918121715 3 1.9988658048927714 4.677912959525585 4 0.7895370507692206 2.4636542001491546 5 0.37651733190011055 1.4685955922855698 6 0.225011419229633 1.0531810904754662 7 0.13886438931378597 0.7582914726912496 8 0.08742316566508124 0.5455866803749728 9 0.07119970508341675 0.49988251683856605 >10 0.3149443186662468 4.271797605104438 >50 0.01477665141013031 0.7866327993401725 >100 0.00561032034582096 0.8258407539517192 >500 1.2196317253433333E-4 0.05870311139443505 >1k 4.8785269013733334E-4 1.0999459818979962 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4002 0.23164373864427637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3969 0.22973363285335655 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2313 0.13388105134538011 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1576398732847395E-4 2 0.0 0.0 0.0 0.0 1.7364598099271091E-4 3 0.0 0.0 0.0 0.0 1.7364598099271091E-4 4 0.0 0.0 0.0 0.0 1.7364598099271091E-4 5 0.0 0.0 0.0 0.0 1.7364598099271091E-4 6 0.0 0.0 0.0 0.0 2.8940996832118487E-4 7 0.0 0.0 0.0 0.0 2.8940996832118487E-4 8 0.0 0.0 0.0 0.0 2.8940996832118487E-4 9 0.0 0.0 0.0 5.7881993664236976E-5 2.8940996832118487E-4 10-11 0.0 0.0 0.0 5.7881993664236976E-5 3.4729196198542183E-4 12-13 0.0 0.0 0.0 5.7881993664236976E-5 5.498789398102513E-4 14-15 0.0 0.0 0.0 5.7881993664236976E-5 6.077609334744882E-4 16-17 0.0 0.0 0.0 5.7881993664236976E-5 6.945839239708437E-4 18-19 5.7881993664236976E-5 0.0 0.0 5.7881993664236976E-5 8.103479112993176E-4 20-21 1.1576398732847395E-4 0.0 0.0 8.682299049635547E-5 8.682299049635546E-4 22-23 1.1576398732847395E-4 0.0 0.0 2.315279746569479E-4 9.550528954599101E-4 24-25 1.7364598099271091E-4 0.0 0.0 2.8940996832118487E-4 9.839938922920285E-4 26-27 1.7364598099271091E-4 0.0 0.0 6.077609334744882E-4 0.0010997578796205026 28-29 1.7364598099271091E-4 0.0 0.0 0.0010418758859562656 0.0011576398732847395 30-31 1.7364598099271091E-4 0.0 0.0 0.003472919619854219 0.0011576398732847395 32-33 1.7364598099271091E-4 0.0 0.0 0.010331935869066299 0.0011576398732847395 34-35 2.604689714890664E-4 0.0 0.0 0.021676806627256746 0.0011576398732847395 36-37 2.8940996832118487E-4 0.0 0.0 0.042543265343214176 0.0011576398732847395 38-39 2.8940996832118487E-4 0.0 0.0 0.07649105462728917 0.001186580870116858 40-41 2.8940996832118487E-4 0.0 0.0 0.11243577269278032 0.0012155218669489764 42-43 3.4729196198542183E-4 0.0 0.0 0.1493644846505635 0.0013602268511095689 44-45 3.4729196198542183E-4 0.0 0.0 0.19650936849008452 0.0014470498416059244 46-47 4.919969461460143E-4 0.0 0.0 0.2573433438311976 0.0015049318352701613 48-49 5.788199366423697E-4 0.0 0.0 0.32347352159258835 0.0015628138289343983 50-51 5.788199366423697E-4 0.0 0.0 0.3938869668851326 0.0016785778162628723 52-53 5.788199366423697E-4 0.0 0.0 0.4661526359749325 0.0018522237972555832 54-55 6.367019303066067E-4 0.0 0.0 0.5462034332125723 0.0019101057909198201 56-57 6.945839239708437E-4 0.0 0.0 0.6442555304797897 0.0019101057909198201 58-59 6.945839239708437E-4 0.0 0.0 0.7515398057364528 0.0019101057909198201 60-61 6.945839239708437E-4 0.0 0.0 0.8717896475739052 0.0019101057909198201 62-63 6.945839239708437E-4 0.0 0.0 0.9944994741420876 0.001967987784584057 64-65 6.945839239708437E-4 0.0 0.0 1.119408816469511 0.0019969287814161755 66-67 6.945839239708437E-4 0.0 0.0 1.2685128321485855 0.0020837517719125313 68-69 6.945839239708437E-4 0.0 0.0 1.4429112790589316 0.0021705747624088867 70-71 6.945839239708437E-4 0.0 0.0 1.6388418276123735 0.0022863387497373605 72-73 6.945839239708437E-4 0.0 0.0 1.8413419824467065 0.0026046897148906635 74-75 6.945839239708437E-4 0.0 0.0 2.0450576591479885 0.0027783356958833746 76-77 6.945839239708437E-4 0.0 0.0 2.271347313378323 0.0028362176895476115 78-79 6.945839239708437E-4 0.0 0.0 2.5398329409898865 0.0029230406800439673 80-81 8.39288908131436E-4 0.0 0.0 2.838504028297349 0.0030098636705403227 82-83 8.682299049635546E-4 0.0 0.0 3.14452612880017 0.0032992736388615077 84-85 8.682299049635546E-4 0.0 0.0 3.4482908315500858 0.003328214635693626 86-87 8.682299049635546E-4 0.0 0.0 3.77853654640139 0.0034729196198542184 88-89 8.682299049635546E-4 0.0 0.0 4.1374049071196595 0.0036465656008469296 90-91 9.261118986277916E-4 0.0 0.0 4.54110287193088 0.0037044475945111665 92-93 9.261118986277916E-4 0.0 0.0 4.969719035014554 0.003878093575503877 94-95 9.261118986277916E-4 0.0 0.0 5.395035924459368 0.003907034572335996 96-97 9.261118986277916E-4 0.0 0.0 5.833231557494473 0.004283267531153536 98-99 9.261118986277916E-4 0.0 0.0 6.309976598309961 0.004688441486803195 100-101 0.0010129348891241472 0.0 0.0 6.846687384561599 0.0048620874677959055 102-103 0.0012155218669489764 0.0 0.0 7.405480151396143 0.004919969461460142 104-105 0.0013312858542774504 0.0 0.0 7.968064188815694 0.004977851455124379 106-107 0.0013891678479416873 0.0 0.0 8.520084762391521 0.004977851455124379 108-109 0.001418108844773806 0.0 0.0 9.112767436516476 0.004977851455124379 110-111 0.001475990838438043 0.0 0.0 9.746604208136704 0.0051225564392849724 112-113 0.0015628138289343983 0.0 0.0 10.42660186970416 0.005151497436117091 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2820 0.0 73.35618 1 GTATCAA 5030 0.0 50.489136 1 ATCAACG 6490 0.0 39.143417 3 TCAACGC 6530 0.0 38.722546 4 TATCAAC 6735 0.0 37.80892 2 CAACGCA 6710 0.0 37.683784 5 AACGCAG 6950 0.0 36.46914 6 GTGGTAT 1440 0.0 34.36148 1 TGGTATC 1450 0.0 33.235725 2 GTACATG 5955 0.0 31.734655 1 ACGCAGA 8235 0.0 30.781118 7 TACATGG 6145 0.0 30.30474 2 CGCAGAG 8350 0.0 30.214666 8 ACATGGG 6115 0.0 29.771486 3 GCAGAGT 9550 0.0 25.857298 9 GAGTACT 6290 0.0 25.067717 12-13 AGAGTAC 8410 0.0 24.550797 10-11 CAGAGTA 8960 0.0 24.438354 10-11 GTACTTT 6580 0.0 23.779646 14-15 CATGGGG 4225 0.0 23.516783 4 >>END_MODULE