FastQCFastQC Report
Thu 26 May 2016
SRR1043484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043484_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences37875
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7271.9194719471947195No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC4741.2514851485148515No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC1900.5016501650165017No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC1260.3326732673267327No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC910.24026402640264025No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC760.20066006600660066No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.17953795379537954No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG560.14785478547854786No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA560.14785478547854786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCAGG253.819533E-545.0000041
AGAGGGC253.819533E-545.0000043
GAATGAT253.819533E-545.0000048
GAAGGGC253.819533E-545.0000043
ATGATAC206.947081E-445.010
ATAGGGA206.947081E-445.03
ATTGGGC206.947081E-445.03
AGGGCGC206.947081E-445.05
ATGCGGG206.947081E-445.02
CAGAGGG453.6379788E-1045.02
GACGAGG206.947081E-445.01
AACAGGG206.947081E-445.02
TAGGGCA206.947081E-445.04
GAGGGGT206.947081E-445.04
TGCAGGG453.6379788E-1045.02
TGCGGGA206.947081E-445.03
GGGCGCG551.8189894E-1244.9999966
GAGGGCA302.1101896E-644.9999964
GCGAGGG302.1101896E-644.9999962
CGTTTTT2350.043.0851061