##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043484_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	37875
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46693069306931	31.0	31.0	33.0	30.0	34.0
2	30.569742574257425	31.0	31.0	34.0	26.0	34.0
3	31.543102310231024	31.0	31.0	34.0	28.0	34.0
4	35.16520132013201	37.0	35.0	37.0	32.0	37.0
5	35.493993399339935	37.0	35.0	37.0	33.0	37.0
6	35.60596699669967	37.0	35.0	37.0	35.0	37.0
7	33.63630363036304	37.0	35.0	37.0	31.0	37.0
8	34.64467326732673	37.0	35.0	37.0	31.0	37.0
9	36.72794719471947	39.0	37.0	39.0	32.0	39.0
10	36.68491089108911	39.0	35.0	39.0	32.0	39.0
11	36.408580858085806	38.0	35.0	39.0	32.0	39.0
12	36.39300330033003	39.0	35.0	39.0	32.0	39.0
13	36.419696369636966	39.0	35.0	39.0	32.0	39.0
14	37.626851485148514	40.0	35.0	41.0	33.0	41.0
15	37.68340594059406	40.0	35.0	41.0	33.0	41.0
16	37.439762376237624	40.0	35.0	41.0	32.0	41.0
17	37.41433663366337	40.0	35.0	41.0	32.0	41.0
18	37.278864686468644	39.0	35.0	41.0	32.0	41.0
19	37.17135313531353	39.0	35.0	41.0	32.0	41.0
20	37.03725412541254	39.0	35.0	41.0	32.0	41.0
21	36.93140594059406	38.0	35.0	41.0	32.0	41.0
22	36.84852805280528	38.0	35.0	40.0	32.0	41.0
23	36.81164356435644	38.0	35.0	40.0	32.0	41.0
24	36.68858085808581	38.0	35.0	40.0	31.0	41.0
25	36.55672607260726	38.0	35.0	40.0	31.0	41.0
26	36.29185478547855	38.0	35.0	40.0	31.0	41.0
27	36.15574917491749	38.0	35.0	40.0	30.0	41.0
28	35.94759075907591	37.0	35.0	40.0	30.0	41.0
29	35.81829702970297	38.0	34.0	40.0	30.0	41.0
30	35.634957095709574	37.0	34.0	40.0	29.0	41.0
31	35.555405940594056	38.0	34.0	40.0	29.0	41.0
32	35.53174917491749	38.0	34.0	40.0	29.0	41.0
33	35.276514851485146	37.0	34.0	40.0	27.0	41.0
34	35.14484488448845	37.0	34.0	40.0	26.0	41.0
35	35.06526732673267	37.0	34.0	40.0	26.0	41.0
36	34.9720396039604	37.0	34.0	40.0	26.0	41.0
37	34.862468646864684	37.0	34.0	40.0	25.0	41.0
38	34.715458745874585	37.0	34.0	40.0	24.0	41.0
39	34.59680528052805	36.0	34.0	40.0	24.0	41.0
40	34.47081188118812	36.0	34.0	40.0	24.0	41.0
41	34.39738613861386	36.0	33.0	40.0	24.0	41.0
42	34.2579801980198	35.0	33.0	40.0	23.0	41.0
43	34.078204620462046	35.0	33.0	40.0	23.0	41.0
44	33.88607260726073	35.0	33.0	40.0	23.0	41.0
45	33.61454785478548	35.0	33.0	40.0	22.0	41.0
46	33.604726072607264	35.0	33.0	39.0	23.0	41.0
47	33.5486204620462	35.0	33.0	39.0	23.0	41.0
48	33.45285808580858	35.0	33.0	39.0	23.0	41.0
49	33.403036303630365	35.0	33.0	39.0	23.0	41.0
50	33.34574257425743	35.0	33.0	39.0	23.0	41.0
51	32.88113531353135	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	3.0
15	3.0
16	3.0
17	6.0
18	31.0
19	33.0
20	76.0
21	103.0
22	184.0
23	228.0
24	350.0
25	469.0
26	679.0
27	740.0
28	841.0
29	974.0
30	1076.0
31	1183.0
32	1448.0
33	2121.0
34	3799.0
35	3703.0
36	3628.0
37	4413.0
38	5577.0
39	6201.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.29438943894389	18.518811881188117	23.931353135313532	27.25544554455446
2	30.363036303630363	21.05082508250825	26.062046204620458	22.52409240924092
3	27.97623762376238	22.822442244224423	28.6019801980198	20.5993399339934
4	24.712871287128714	25.555115511551158	26.55313531353135	23.17887788778878
5	22.48712871287129	35.6013201320132	24.293069306930693	17.61848184818482
6	86.46600660066007	1.9564356435643564	10.307590759075907	1.26996699669967
7	82.21782178217822	7.007260726072608	9.526072607260726	1.2488448844884488
8	82.05148514851484	3.326732673267327	11.580198019801982	3.0415841584158416
9	47.255445544554455	17.951155115511554	18.138613861386137	16.654785478547854
10	32.3009900990099	18.114851485148513	26.405280528052806	23.17887788778878
11	29.87194719471947	14.571617161716171	34.735313531353135	20.82112211221122
12	25.09042904290429	16.07392739273927	35.24752475247524	23.588118811881188
13	19.973597359735972	20.921452145214523	38.2970297029703	20.80792079207921
14	20.895049504950496	18.727392739273927	37.5023102310231	22.875247524752478
15	26.67722772277228	14.059405940594061	36.34323432343234	22.920132013201318
16	24.76831683168317	16.314191419141913	33.560396039603965	25.35709570957096
17	25.919471947194722	20.615181518151815	30.735313531353135	22.730033003300328
18	26.130693069306933	26.01980198019802	27.26864686468647	20.580858085808583
19	29.14587458745875	21.57095709570957	26.78283828382838	22.500330033003298
20	29.09306930693069	23.918151815181517	29.21188118811881	17.77689768976898
21	23.68052805280528	18.801320132013203	32.121452145214526	25.396699669967
22	26.851485148514854	20.691749174917494	30.73267326732673	21.724092409240924
23	29.169636963696373	20.57029702970297	30.088448844884493	20.17161716171617
24	30.172937293729372	17.47326732673267	27.300330033003302	25.053465346534654
25	23.21848184818482	20.26138613861386	29.68976897689769	26.830363036303627
26	21.005940594059407	18.92013201320132	32.6970297029703	27.376897689768974
27	23.71221122112211	22.14125412541254	31.149834983498348	22.996699669966997
28	25.499669966996702	24.451485148514852	28.034323432343232	22.014521452145214
29	25.533993399339934	19.44818481848185	33.932673267326734	21.085148514851486
30	31.339933993399338	19.107590759075908	29.029702970297027	20.522772277227723
31	31.08910891089109	20.93729372937294	26.7036303630363	21.269966996699672
32	31.693729372937295	18.954455445544554	25.82970297029703	23.52211221122112
33	26.069966996699666	21.594719471947194	29.71089108910891	22.624422442244224
34	24.937293729372936	23.765016501650166	28.374917491749173	22.922772277227725
35	27.184158415841587	27.033663366336636	24.46996699669967	21.312211221122112
36	27.165676567656767	24.92145214521452	23.801980198019802	24.11089108910891
37	26.69042904290429	29.67656765676568	22.56633663366337	21.066666666666666
38	27.036303630363033	23.91023102310231	22.471287128712873	26.582178217821784
39	24.947854785478548	21.174917491749177	29.04026402640264	24.836963696369637
40	22.603300330033	23.865346534653465	28.26666666666667	25.264686468646865
41	21.357095709570956	28.195379537953798	23.696369636963695	26.751155115511548
42	21.306930693069308	21.098349834983498	25.54719471947195	32.04752475247525
43	23.052145214521452	19.786138613861386	30.18085808580858	26.980858085808578
44	27.332013201320134	22.075247524752477	27.141914191419144	23.45082508250825
45	27.84422442244224	22.822442244224423	27.01254125412541	22.320792079207923
46	23.894389438943893	24.227062706270626	26.627062706270628	25.251485148514853
47	22.196699669966996	26.489768976897686	28.425082508250828	22.888448844884486
48	26.31815181518152	24.792079207920793	26.9016501650165	21.98811881188119
49	23.437623762376237	21.10891089108911	30.0963696369637	25.35709570957096
50	21.724092409240924	23.16831683168317	32.38019801980198	22.727392739273927
51	21.90891089108911	20.332673267326733	30.233663366336632	27.524752475247528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	89.5
2	171.0
3	695.5
4	1220.0
5	767.5
6	315.0
7	274.5
8	234.0
9	226.5
10	219.0
11	229.5
12	240.0
13	224.5
14	209.0
15	209.0
16	209.0
17	186.0
18	163.0
19	164.0
20	165.0
21	167.5
22	170.0
23	162.0
24	154.0
25	157.0
26	161.0
27	162.0
28	193.0
29	224.0
30	276.0
31	328.0
32	336.5
33	345.0
34	427.0
35	509.0
36	527.0
37	545.0
38	608.5
39	672.0
40	719.0
41	766.0
42	893.5
43	1021.0
44	1141.0
45	1261.0
46	1374.0
47	1487.0
48	1763.5
49	2040.0
50	2117.5
51	2195.0
52	1962.5
53	1730.0
54	1851.5
55	1973.0
56	2247.5
57	2522.0
58	2655.0
59	2788.0
60	2814.0
61	2840.0
62	2899.0
63	2958.0
64	2669.0
65	2380.0
66	1995.5
67	1611.0
68	1342.0
69	1073.0
70	954.0
71	835.0
72	822.0
73	809.0
74	699.0
75	445.5
76	302.0
77	219.5
78	137.0
79	106.0
80	75.0
81	56.0
82	37.0
83	26.0
84	15.0
85	10.0
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	37875.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.21122112211222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45747445002598	63.603960396039604
2	12.191235059760956	18.58217821782178
3	3.0452104624978347	6.962376237623763
4	0.6894162480512732	2.1016501650165016
5	0.22518621167503897	0.8580858085808581
6	0.11432530746578902	0.5227722772277228
7	0.03810843582192967	0.20330033003300332
8	0.04157283907846873	0.25346534653465347
9	0.017322016282695307	0.1188118811881188
>10	0.14896934003117965	1.871947194719472
>50	0.017322016282695307	0.9161716171617161
>100	0.010393209769617183	2.0858085808580857
>500	0.0034644032565390613	1.9194719471947195
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	727	1.9194719471947195	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	474	1.2514851485148515	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	190	0.5016501650165017	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	126	0.3326732673267327	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	91	0.24026402640264025	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	76	0.20066006600660066	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.17953795379537954	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	56	0.14785478547854786	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	56	0.14785478547854786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013201320132013201	0.0
2	0.0	0.0	0.0	0.044884488448844885	0.0
3	0.0	0.0	0.0	0.07392739273927393	0.0
4	0.0	0.0	0.0	0.16897689768976898	0.0
5	0.0	0.0	0.0	0.35115511551155115	0.0
6	0.0	0.0	0.0	0.38811881188118813	0.0
7	0.0	0.0	0.0	0.5386138613861386	0.0
8	0.0	0.0	0.0	0.6706270627062706	0.0
9	0.0	0.0	0.0	0.8158415841584158	0.0
10	0.0	0.0	0.0	0.8897689768976897	0.0
11	0.0	0.0	0.0	0.9795379537953796	0.0
12	0.0	0.0	0.0	1.0402640264026404	0.0
13	0.0	0.0	0.0	1.0534653465346535	0.0
14	0.0	0.0	0.0	1.0772277227722773	0.0
15	0.0	0.0	0.0	1.1511551155115511	0.0
16	0.0	0.0	0.0	1.2462046204620463	0.0
17	0.0	0.0	0.0	1.3465346534653466	0.0
18	0.0	0.0	0.0	1.4046204620462046	0.0
19	0.0	0.0	0.0	1.4732673267326732	0.0
20	0.0	0.0	0.0	1.5419141914191419	0.0
21	0.0	0.0	0.0	1.6237623762376239	0.0
22	0.0	0.0	0.0	1.6976897689768977	0.0
23	0.0	0.0	0.0	1.768976897689769	0.0
24	0.0	0.0	0.0	1.813861386138614	0.0
25	0.0	0.0	0.0	1.871947194719472	0.0
26	0.0	0.0	0.0	1.937953795379538	0.0
27	0.0	0.0	0.0	2.01980198019802	0.0
28	0.0	0.0	0.0	2.0937293729372937	0.0
29	0.0	0.0	0.0	2.1755775577557754	0.0
30	0.0	0.0	0.0	2.2547854785478547	0.0
31	0.0	0.0	0.0	2.3155115511551156	0.0
32	0.0	0.0	0.0	2.378877887788779	0.0
33	0.0	0.0	0.0	2.4633663366336633	0.0
34	0.0	0.0	0.0	2.568976897689769	0.0
35	0.0	0.0	0.0	2.6745874587458744	0.0
36	0.0	0.0	0.0	2.798679867986799	0.0
37	0.0	0.0	0.0	2.9465346534653465	0.0
38	0.0	0.0	0.0	3.104950495049505	0.0
39	0.0	0.0	0.0	3.2607260726072607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCAGG	25	3.819533E-5	45.000004	1
AGAGGGC	25	3.819533E-5	45.000004	3
GAATGAT	25	3.819533E-5	45.000004	8
GAAGGGC	25	3.819533E-5	45.000004	3
ATGATAC	20	6.947081E-4	45.0	10
ATAGGGA	20	6.947081E-4	45.0	3
ATTGGGC	20	6.947081E-4	45.0	3
AGGGCGC	20	6.947081E-4	45.0	5
ATGCGGG	20	6.947081E-4	45.0	2
CAGAGGG	45	3.6379788E-10	45.0	2
GACGAGG	20	6.947081E-4	45.0	1
AACAGGG	20	6.947081E-4	45.0	2
TAGGGCA	20	6.947081E-4	45.0	4
GAGGGGT	20	6.947081E-4	45.0	4
TGCAGGG	45	3.6379788E-10	45.0	2
TGCGGGA	20	6.947081E-4	45.0	3
GGGCGCG	55	1.8189894E-12	44.999996	6
GAGGGCA	30	2.1101896E-6	44.999996	4
GCGAGGG	30	2.1101896E-6	44.999996	2
CGTTTTT	235	0.0	43.085106	1
>>END_MODULE