##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043483_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3999678
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.576455154639948	31.0	31.0	33.0	30.0	34.0
2	30.583572727604572	31.0	31.0	34.0	26.0	34.0
3	31.551979684364593	31.0	31.0	34.0	28.0	34.0
4	35.17373998606888	37.0	35.0	37.0	32.0	37.0
5	35.50558819985009	37.0	35.0	37.0	33.0	37.0
6	35.837056633058964	37.0	35.0	37.0	35.0	37.0
7	33.699367549087704	37.0	35.0	37.0	32.0	37.0
8	34.75653340093878	37.0	35.0	37.0	31.0	37.0
9	36.79428869023956	39.0	37.0	39.0	32.0	39.0
10	36.802119070585185	39.0	35.0	39.0	32.0	39.0
11	36.83647808648596	39.0	35.0	39.0	33.0	39.0
12	36.878144690647595	39.0	37.0	39.0	33.0	39.0
13	36.914411610134614	39.0	37.0	39.0	33.0	39.0
14	38.25383118341026	40.0	37.0	41.0	33.0	41.0
15	38.31770057489628	40.0	37.0	41.0	33.0	41.0
16	38.212049319970255	40.0	37.0	41.0	33.0	41.0
17	38.17824034834804	40.0	37.0	41.0	33.0	41.0
18	37.89320390291419	39.0	37.0	41.0	33.0	41.0
19	37.62389372344474	39.0	36.0	41.0	33.0	41.0
20	37.31654048150876	39.0	35.0	41.0	33.0	41.0
21	37.21817331295169	39.0	35.0	41.0	33.0	41.0
22	37.12460628080561	39.0	35.0	41.0	33.0	41.0
23	37.0501332857295	38.0	35.0	41.0	33.0	41.0
24	36.90402227379304	38.0	35.0	41.0	32.0	41.0
25	36.81387476691874	38.0	35.0	40.0	32.0	41.0
26	36.53534709544118	38.0	35.0	40.0	31.0	41.0
27	36.416047991863344	38.0	35.0	40.0	31.0	41.0
28	36.25693318312124	38.0	35.0	40.0	31.0	41.0
29	36.20051239124749	38.0	35.0	40.0	31.0	41.0
30	36.03118401031283	38.0	35.0	40.0	30.0	41.0
31	35.69826446028905	38.0	35.0	40.0	29.0	41.0
32	35.544486331149656	38.0	35.0	40.0	27.0	41.0
33	35.320434795001	38.0	35.0	40.0	26.0	41.0
34	35.11957462575737	38.0	35.0	40.0	24.0	41.0
35	34.89832281498661	38.0	35.0	40.0	23.0	41.0
36	34.74821498130599	38.0	34.0	40.0	23.0	41.0
37	34.61076241637452	38.0	34.0	40.0	22.0	41.0
38	34.46810518246719	38.0	34.0	40.0	21.0	41.0
39	34.33563076827684	37.0	34.0	40.0	21.0	41.0
40	34.21172929420818	37.0	34.0	40.0	20.0	41.0
41	34.09924173895999	37.0	33.0	40.0	20.0	41.0
42	33.98752699592317	37.0	33.0	40.0	20.0	41.0
43	33.81104053876337	36.0	33.0	40.0	18.0	41.0
44	33.608072699852336	36.0	33.0	40.0	18.0	41.0
45	33.399101877701156	36.0	33.0	40.0	18.0	41.0
46	33.40293218604098	36.0	33.0	40.0	18.0	41.0
47	33.35865812197882	35.0	33.0	40.0	18.0	41.0
48	33.27164136713005	35.0	33.0	40.0	18.0	41.0
49	33.22478659532092	35.0	33.0	40.0	18.0	41.0
50	33.14184566857632	35.0	33.0	40.0	18.0	41.0
51	32.737210595452936	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	8.0
12	20.0
13	27.0
14	97.0
15	155.0
16	411.0
17	966.0
18	2062.0
19	3781.0
20	6641.0
21	10721.0
22	16596.0
23	26277.0
24	41243.0
25	63319.0
26	84820.0
27	97642.0
28	99864.0
29	99504.0
30	104469.0
31	117690.0
32	140669.0
33	181782.0
34	291561.0
35	365851.0
36	413282.0
37	431246.0
38	575115.0
39	823422.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.437008679198676	16.760224198047943	23.18076605166716	32.622001071086224
2	34.41082007101572	19.412287689158976	26.369947780796355	19.80694445902895
3	24.131892617355696	20.638436394129727	36.943623961728925	18.286047026785656
4	22.269542698187202	23.374606655835795	34.11402117870488	20.241829467272115
5	20.59080756000858	31.566491102533757	32.0934835254238	15.74921781203387
6	77.85174206523625	1.9861348838581507	18.79136270469773	1.3707603462078697
7	73.46376383298856	7.43154823963329	17.60449216161901	1.5001957657591436
8	72.38640210536948	4.176386199089026	20.713692452242405	2.723519243299086
9	35.00441785563738	25.833079562904814	26.849136355476617	12.313366225981191
10	22.663524413715304	20.90590792558801	38.63863541015052	17.791932250546168
11	21.368295147759394	17.455530170178697	43.226279715517094	17.949894966544807
12	20.24685487181718	18.294347694989447	43.44232210692961	18.016475326263766
13	17.534936562393273	22.55046531245765	42.115465294956245	17.79913283019283
14	16.752848604312646	21.765202098768953	42.59020351138267	18.891745785535736
15	20.08066649365274	18.238593206753144	43.026663646423536	18.65407665317058
16	19.99575965865252	19.08861163323648	39.5158060223848	21.3998226857262
17	19.51929630335242	21.313965774244828	39.68786987352482	19.478868048877935
18	21.042518922773283	22.75198153451353	37.79849277866869	18.407006764044507
19	21.647167596991558	21.237534621537034	37.40428604502662	19.71101173644478
20	22.42833048060369	22.086577969526548	39.05866922287244	16.426422326997322
21	20.290258365798447	19.290902917684875	38.80509881045424	21.613739906062438
22	18.913422530513706	22.121930815430645	38.94638518400731	20.01826147004834
23	19.121089247684438	21.295589294938242	41.0436790161608	18.539642441216518
24	21.91621425524755	20.44009542768193	38.80964917675873	18.834041140311793
25	19.114613726404976	22.440406452719444	37.60147691889197	20.84350290198361
26	18.442759642151195	21.759176613717404	39.42454867616843	20.373515067962973
27	19.15776720025962	21.89976293091594	40.488984363241244	18.4534855055832
28	19.630030217432505	22.673475214754788	38.92988385565038	18.76661071216233
29	19.439589886985903	21.086897495248365	40.616644639893515	18.85686797787222
30	22.324022083777745	21.870585582139363	37.180117999498954	18.62527433458393
31	21.0825971490705	22.848614313452234	37.146190268316595	18.922598269160666
32	21.5201573726685	21.571011466423045	36.40623070157148	20.502600459336975
33	19.214396758939095	22.813061451446842	36.9741764212019	20.99836536841216
34	19.823820817575815	22.0570505925727	36.907746073558926	21.211382516292563
35	22.239565285005444	23.713758957596088	34.73044580088697	19.3162299565115
36	20.11024387463191	24.2748041217318	34.35134028289277	21.26361172074352
37	20.92400938275531	26.72550140286293	33.150543618761304	19.199945595620445
38	20.596933053110774	24.188847202199774	32.381006671037014	22.833213073652427
39	20.791348703570637	23.191066880883913	36.16496127938299	19.85262313616246
40	19.39928664257473	23.231945171586315	36.138584156024564	21.2301840298144
41	19.494494306791697	26.38239878310204	32.895423081558064	21.227683828548198
42	19.593177250768687	23.308276316243457	34.362116150350104	22.736430282637752
43	19.9306044136553	23.093108995274118	36.190513336323576	20.785773254747006
44	21.024367461580656	24.19707286436558	34.799451355834144	19.979108318219616
45	21.72467383624382	23.58599867289317	34.380417623618705	20.308909867244314
46	20.64131162558586	24.56292731564891	34.002212178080335	20.793548880684895
47	19.412787729412216	26.326719300903722	34.48947640285043	19.77101656683363
48	20.032062581037774	25.023364380832657	34.38604307646765	20.558529961661915
49	19.434689492504145	23.357230257035692	36.36675252357815	20.841327726882014
50	18.988778596677033	24.038660112139027	36.81513861865881	20.157422672525136
51	18.952850704481712	22.546990032697632	35.84696068033477	22.65319858248589
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1635.0
1	21320.0
2	41005.0
3	164191.0
4	287377.0
5	173124.0
6	58871.0
7	53414.0
8	47957.0
9	48169.5
10	48382.0
11	48046.0
12	47710.0
13	46404.5
14	45099.0
15	42482.5
16	39866.0
17	37532.0
18	35198.0
19	33108.0
20	31018.0
21	30212.5
22	29407.0
23	29776.5
24	30146.0
25	32511.0
26	39031.5
27	43187.0
28	47662.0
29	52137.0
30	57501.5
31	62866.0
32	70603.0
33	78340.0
34	85293.0
35	92246.0
36	99409.0
37	106572.0
38	112574.5
39	118577.0
40	127565.0
41	136553.0
42	149657.5
43	162762.0
44	174153.0
45	185544.0
46	202021.5
47	218499.0
48	243444.5
49	268390.0
50	258977.0
51	249564.0
52	219349.5
53	189135.0
54	183474.5
55	177814.0
56	176285.5
57	174757.0
58	174770.5
59	174784.0
60	168534.0
61	162284.0
62	154716.0
63	147148.0
64	135267.0
65	123386.0
66	104578.5
67	85771.0
68	74034.0
69	62297.0
70	55089.5
71	47882.0
72	44506.5
73	41131.0
74	35140.0
75	21762.5
76	14376.0
77	10980.5
78	7585.0
79	6043.5
80	4502.0
81	3265.0
82	2028.0
83	1503.5
84	979.0
85	725.0
86	471.0
87	374.0
88	277.0
89	166.0
90	55.0
91	53.0
92	51.0
93	36.5
94	22.0
95	15.5
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3999678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.653965068901314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86024617094161	20.486303166274336
2	9.054176165173407	4.826593904684217
3	3.1007844363218107	2.4794460015574313
4	1.5817278758262772	1.6863727820312424
5	1.0372574031600517	1.382351129564365
6	0.7405505427835933	1.1843164979465883
7	0.6101269907218312	1.1383612448805494
8	0.46706766615059725	0.9959364206713024
9	0.4039520172033946	0.9690230660446352
>10	5.044993058262497	31.923721777383328
>50	0.9432902574756702	16.76114457018702
>100	0.14658101946259988	6.020510593284471
>500	0.005368875396821057	0.9414244553851099
>1k	0.0032809794091343495	1.599825316569497
>5k	1.9884723691723327E-4	0.49910733828507375
>10k+	3.9769447383446654E-4	7.105561735250883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206739	5.168891095733207	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	28042	0.701106439068345	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21641	0.5410685560187595	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11709	0.2927485662595839	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9466	0.23666905185867462	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9368	0.23421885461779673	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4675	0.1168844091949402	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4658	0.11645937497968588	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4431	0.1107839181054075	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0508040897292232	0.0
2	0.0	0.0	0.0	0.15411240604868692	0.0
3	0.0	0.0	0.0	0.25757073444412276	0.0
4	0.0	0.0	0.0	0.3303515933032609	0.0
5	0.0	0.0	0.0	0.6900055454464085	0.0
6	0.0	0.0	0.0	0.8663947447769546	0.0
7	0.0	0.0	0.0	1.2577012449502185	0.0
8	0.0	0.0	0.0	1.6274810122214838	0.0
9	0.0	0.0	0.0	1.989435149529537	0.0
10	0.0	0.0	0.0	2.2080777502588957	0.0
11	0.0	0.0	0.0	2.4419715787120864	0.0
12	0.0	0.0	0.0	2.640112529058589	0.0
13	0.0	0.0	0.0	2.7386704629722693	0.0
14	0.0	0.0	0.0	2.8103262312616164	0.0
15	0.0	0.0	0.0	2.999966497303033	0.0
16	0.0	0.0	0.0	3.220259230868085	0.0
17	2.5002012662019293E-5	0.0	0.0	3.4972065251252724	0.0
18	2.5002012662019293E-5	0.0	0.0	3.6513939372119455	0.0
19	2.5002012662019293E-5	0.0	0.0	3.8340086376953346	0.0
20	2.5002012662019293E-5	0.0	0.0	4.030299439104848	0.0
21	2.5002012662019293E-5	0.0	0.0	4.23226569738864	0.0
22	7.500603798605788E-5	0.0	0.0	4.4521834007637615	0.0
23	7.500603798605788E-5	0.0	0.0	4.643798825805478	0.0
24	1.0000805064807717E-4	0.0	0.0	4.808062048994944	0.0
25	1.2501006331009647E-4	0.0	0.0	4.964349630145227	0.0
26	1.2501006331009647E-4	0.0	0.0	5.14268898646341	0.0
27	1.2501006331009647E-4	0.0	0.0	5.3322542464668405	0.0
28	1.5001207597211576E-4	0.0	0.0	5.531120255180542	0.0
29	1.7501408863413505E-4	0.0	0.0	5.741837217896041	0.0
30	1.7501408863413505E-4	0.0	0.0	5.9550793838904035	0.0
31	1.7501408863413505E-4	0.0	0.0	6.162746101061136	0.0
32	1.7501408863413505E-4	0.0	0.0	6.385314017778431	0.0
33	1.7501408863413505E-4	0.0	0.0	6.604156634609086	0.0
34	1.7501408863413505E-4	0.0	0.0	6.858802133571753	0.0
35	1.7501408863413505E-4	0.0	0.0	7.08424528174518	0.0
36	1.7501408863413505E-4	0.0	0.0	7.334240406352712	0.0
37	1.7501408863413505E-4	0.0	0.0	7.590586042176395	0.0
38	1.7501408863413505E-4	0.0	0.0	7.855757388469772	0.0
39	1.7501408863413505E-4	0.0	0.0	8.162107049617495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	52915	0.0	43.919964	1
CGACGGT	1160	0.0	42.86638	27
CGGTCTA	1205	0.0	41.265556	30
ACTACGG	240	0.0	41.249996	1
TCACGAC	1230	0.0	40.97561	24
CGTTTTA	2905	0.0	40.817554	1
CGCACTT	9225	0.0	40.78049	34
CACTTAC	9310	0.0	40.529	36
GCACTTA	9300	0.0	40.475807	35
ACTTACT	9270	0.0	40.26699	37
CCGCACT	9355	0.0	40.26189	33
CACGACG	1250	0.0	40.140003	25
CCCGCAC	9460	0.0	40.10042	32
TACGGGA	1320	0.0	40.056816	3
ACCCGCA	9445	0.0	39.925884	31
ACGGGAT	1510	0.0	39.63576	4
CTTACTG	9400	0.0	39.56649	38
TCAACGC	9940	0.0	39.295773	15
CTTATGA	9805	0.0	39.12545	25
CAACGCA	10000	0.0	39.037502	16
>>END_MODULE