##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043481_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3146857
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473448904732564	31.0	31.0	33.0	30.0	34.0
2	30.48067262033197	31.0	31.0	34.0	26.0	34.0
3	31.47598635718115	31.0	31.0	34.0	28.0	34.0
4	35.09505071250457	37.0	35.0	37.0	32.0	37.0
5	35.42581153195077	37.0	35.0	37.0	33.0	37.0
6	35.72024848920685	37.0	35.0	37.0	35.0	37.0
7	33.59789974568275	37.0	35.0	37.0	31.0	37.0
8	34.645403969738695	37.0	35.0	37.0	31.0	37.0
9	36.676956722215216	39.0	37.0	39.0	32.0	39.0
10	36.67115315376581	39.0	35.0	39.0	32.0	39.0
11	36.45178983347512	39.0	35.0	39.0	32.0	39.0
12	36.420851980245686	39.0	35.0	39.0	32.0	39.0
13	36.40263157811111	39.0	35.0	39.0	32.0	39.0
14	37.60067298895374	40.0	36.0	41.0	33.0	41.0
15	37.70854315909493	40.0	36.0	41.0	33.0	41.0
16	37.522874093103056	40.0	35.0	41.0	32.0	41.0
17	37.48495816619567	40.0	36.0	41.0	32.0	41.0
18	37.33154286959973	39.0	35.0	41.0	32.0	41.0
19	37.19500663678076	39.0	35.0	41.0	32.0	41.0
20	36.931314006324406	38.0	35.0	41.0	32.0	41.0
21	36.85910989917877	38.0	35.0	41.0	32.0	41.0
22	36.81877505078877	38.0	35.0	40.0	32.0	41.0
23	36.76559850034495	38.0	35.0	40.0	32.0	41.0
24	36.647174307571014	38.0	35.0	40.0	31.0	41.0
25	36.54540991217586	38.0	35.0	40.0	31.0	41.0
26	36.260558392071836	38.0	35.0	40.0	31.0	41.0
27	36.14368940183809	38.0	35.0	40.0	30.0	41.0
28	35.96671154742653	37.0	35.0	40.0	30.0	41.0
29	35.90064340387885	37.0	35.0	40.0	30.0	41.0
30	35.73934468582462	37.0	34.0	40.0	30.0	41.0
31	35.56434785565407	37.0	34.0	40.0	29.0	41.0
32	35.49789996812693	37.0	34.0	40.0	28.0	41.0
33	35.179186089485476	37.0	34.0	40.0	26.0	41.0
34	35.10801952551387	37.0	34.0	40.0	25.0	41.0
35	35.01355956117485	37.0	34.0	40.0	25.0	41.0
36	34.94366315342578	37.0	34.0	40.0	25.0	41.0
37	34.83800121835851	37.0	34.0	40.0	25.0	41.0
38	34.72792726202684	37.0	34.0	40.0	24.0	41.0
39	34.599569348082866	37.0	34.0	40.0	24.0	41.0
40	34.521961754220165	36.0	34.0	40.0	23.0	41.0
41	34.424533749070896	36.0	34.0	40.0	23.0	41.0
42	34.310318200032604	36.0	33.0	40.0	23.0	41.0
43	34.12970338340764	35.0	33.0	40.0	23.0	41.0
44	33.88714104263397	35.0	33.0	40.0	22.0	41.0
45	33.66085208193445	35.0	33.0	40.0	22.0	41.0
46	33.66435462431245	35.0	33.0	40.0	22.0	41.0
47	33.642085102691354	35.0	33.0	40.0	22.0	41.0
48	33.54865060598559	35.0	33.0	39.0	22.0	41.0
49	33.50574906962725	35.0	33.0	39.0	22.0	41.0
50	33.44418891611535	35.0	33.0	39.0	22.0	41.0
51	33.02843630962576	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	3.0
11	11.0
12	27.0
13	37.0
14	68.0
15	174.0
16	412.0
17	798.0
18	1762.0
19	3206.0
20	5453.0
21	8446.0
22	12968.0
23	19357.0
24	28915.0
25	42407.0
26	55842.0
27	66259.0
28	72918.0
29	79786.0
30	89202.0
31	104204.0
32	125638.0
33	165276.0
34	294083.0
35	315369.0
36	314195.0
37	345192.0
38	435704.0
39	558858.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.517370188731167	18.076353644286982	24.044085892685942	27.362190274295912
2	31.267737936614214	21.152692988591472	27.810129281375033	19.769439793419274
3	27.447449947677953	21.130607460078423	32.31249465736765	19.109447934875973
4	23.31891789172498	24.698516646927395	29.60086842204778	22.381697039299848
5	21.21427824651708	34.2353656362523	27.77949554110657	16.77086057612405
6	82.81351837722528	2.2352143742152886	13.42097845564638	1.5302887929130558
7	78.25366707162098	7.647376413990213	12.552747074303028	1.5462094400857747
8	77.63450960752269	3.8670965982883874	15.242224225632114	3.2561695685568175
9	43.11965875792894	20.287321603746214	21.342787422498066	15.250232215826776
10	30.345706843367843	18.288819606356437	29.669381227046543	21.696092323229177
11	28.074392957798842	15.63957307243386	36.78877050974989	19.497263460017408
12	23.400777347048184	18.96346100251775	36.83059001409978	20.805171636334286
13	18.865744455499566	23.063679093139598	38.704999941211184	19.36557651014965
14	19.081324635978056	19.743413825286627	40.08809424768904	21.08716729104627
15	24.065154533555226	16.099873619932524	38.904214586172806	20.930757260339444
16	23.707845637726784	17.02806959451923	34.60490896154481	24.65917580620918
17	23.63516994893635	20.56480481953899	34.90095037683631	20.899074854688347
18	24.60451809535673	24.434411859197922	31.46104192214645	19.5000281232989
19	27.060397088269344	21.21249869314049	30.180653267689	21.546450950901168
20	25.80276129484117	23.292605923942524	33.50422342038421	17.400409360832096
21	22.798557417766364	19.551094949659294	34.30880399077556	23.341543641798783
22	23.897368072333762	21.779254665845954	32.981924504354666	21.341452757465625
23	24.46046960506944	21.182119174782965	34.595756972750905	19.761654247396688
24	27.203110913524192	18.817346959204055	31.951531321569426	22.028010805702326
25	21.078555523813126	20.970542989401807	32.34309661989725	25.607804866887818
26	18.362130849924228	21.49033146406081	35.39185288686458	24.75568479915039
27	20.516280212288006	22.57681235594754	35.17538292969779	21.731524502066666
28	22.70344664533533	23.877348096847108	31.940917556787614	21.47828770102995
29	22.6513311535923	21.039405349528117	35.52369872542667	20.785564771452915
30	27.25846773463173	21.174238295543777	32.237944081984026	19.329349887840472
31	27.38700868835158	21.4266806531088	30.596655647206084	20.58965501133353
32	27.62791572670763	20.441920303337586	29.457042375932556	22.473121594022228
33	21.989464408455802	22.428187871263294	33.13080321094984	22.451544509331057
34	21.742170044587343	24.58850211496741	31.35064605732005	22.318681783125193
35	25.400582231731534	26.577184791047063	28.188729262244838	19.833503714976565
36	23.00616138578906	25.39263779701461	28.497322884389092	23.103877932807244
37	23.182813836154615	29.069099739835654	27.705866520150106	20.04221990385963
38	22.66750602267596	24.62377540511056	28.067560743942288	24.641157828271194
39	22.538202403223277	21.874778548882265	32.563221017033825	23.023798030860636
40	19.840621928482925	22.390721917138276	32.86564975783774	24.903006396541056
41	20.221033240468188	26.713002846967626	28.059838753397436	25.006125159166746
42	19.708140535143478	21.52639919767565	29.25757350905999	29.507886758120883
43	20.540590182521797	20.780702777406155	33.07754372060758	25.60116331946447
44	23.70972052431998	23.436813302924158	29.434893291941773	23.418572880814096
45	24.052189216097204	24.026322136658894	30.46258536692325	21.45890328032065
46	21.602792881913604	24.03601434701354	30.00320637385175	24.3579863972211
47	20.443286746108896	26.44193873442613	31.651231689269643	21.463542830195333
48	22.874537991399038	24.88762597092909	30.461536701540616	21.77629933613126
49	20.670116246146552	22.294912034452153	33.366466922392725	23.668504797008573
50	20.08909206868949	23.147000324450715	34.61653961397038	22.147367992889414
51	19.373902277732988	21.108712597998576	32.37757546656871	27.139809657699733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1581.0
1	13873.0
2	26165.0
3	89090.0
4	152015.0
5	91500.0
6	30985.0
7	27801.5
8	24618.0
9	24780.5
10	24943.0
11	25159.5
12	25376.0
13	24310.0
14	23244.0
15	21526.5
16	19809.0
17	18678.0
18	17547.0
19	15937.0
20	14327.0
21	13954.0
22	13581.0
23	13834.0
24	14087.0
25	15021.0
26	16861.0
27	17767.0
28	20546.0
29	23325.0
30	27080.0
31	30835.0
32	35668.0
33	40501.0
34	46947.5
35	53394.0
36	57802.5
37	62211.0
38	65884.5
39	69558.0
40	77294.5
41	85031.0
42	97111.0
43	109191.0
44	118380.0
45	127569.0
46	138774.0
47	149979.0
48	179536.5
49	209094.0
50	209331.5
51	209569.0
52	191021.0
53	172473.0
54	175908.0
55	179343.0
56	182020.0
57	184697.0
58	186861.0
59	189025.0
60	190565.5
61	192106.0
62	184771.0
63	177436.0
64	164069.0
65	150702.0
66	126348.5
67	101995.0
68	83391.5
69	64788.0
70	55782.5
71	46777.0
72	44817.0
73	42857.0
74	35043.0
75	20453.5
76	13678.0
77	10169.0
78	6660.0
79	4722.0
80	2784.0
81	2017.5
82	1251.0
83	834.0
84	417.0
85	292.0
86	167.0
87	128.0
88	89.0
89	72.5
90	56.0
91	50.5
92	45.0
93	30.0
94	15.0
95	12.5
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3146857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.303666503466157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.40755872562742	18.812874921151206
2	9.462884122290934	4.599655597382078
3	3.1085721048874344	2.266490992174861
4	1.5274974582853973	1.4849515524424202
5	0.9390776552452722	1.1411515076969032
6	0.6208390326490856	0.9053198881102746
7	0.4237072446019066	0.7208347697535109
8	0.3624249712508551	0.7046604507047274
9	0.30746105753868924	0.6725187904700991
>10	4.127554465918995	25.373863687582965
>50	1.3748517613827655	23.081413336995617
>100	0.32706310432900754	11.645145136552665
>500	0.005884645755681421	0.9332295553058759
>1k	0.0035027653307627507	1.4275626741256746
>5k	5.604424529220401E-4	0.9528435166229665
>10k+	5.604424529220401E-4	5.277483622928212
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101694	3.2316053764120833	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	29171	0.926988420509734	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12813	0.40716816811186524	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11305	0.35924733789937074	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9429	0.2996322997835618	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	6603	0.20982840974343606	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6599	0.20970129878796528	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5351	0.1700426806810732	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4534	0.1440802680261607	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04404394607063492	0.0
2	0.0	0.0	0.0	0.14598693235822283	0.0
3	0.0	0.0	0.0	0.2265117226489796	0.0
4	0.0	0.0	0.0	0.28237698757839963	0.0
5	0.0	0.0	0.0	0.6219856828575305	0.0
6	0.0	0.0	0.0	0.724087557839457	0.0
7	0.0	0.0	0.0	1.0146949797845914	0.0
8	0.0	0.0	0.0	1.3367305854698832	0.0
9	0.0	0.0	0.0	1.6127202475358746	0.0
10	0.0	0.0	0.0	1.8130153356190002	0.0
11	3.1777738867701964E-5	0.0	0.0	2.0332668437110426	0.0
12	3.1777738867701964E-5	0.0	0.0	2.21735528497164	0.0
13	6.355547773540393E-5	0.0	0.0	2.3063647315400733	0.0
14	6.355547773540393E-5	0.0	0.0	2.372811983512438	0.0
15	6.355547773540393E-5	0.0	0.0	2.5861359445313212	0.0
16	6.355547773540393E-5	0.0	0.0	2.8235474316119227	0.0
17	6.355547773540393E-5	0.0	0.0	3.1385283792685845	0.0
18	6.355547773540393E-5	0.0	0.0	3.3083168380387162	0.0
19	6.355547773540393E-5	0.0	0.0	3.5093428141157985	0.0
20	6.355547773540393E-5	0.0	0.0	3.7417016407164354	0.0
21	6.355547773540393E-5	0.0	0.0	3.9699929167420063	0.0
22	6.355547773540393E-5	0.0	0.0	4.203050853597732	0.0
23	9.53332166031059E-5	0.0	0.0	4.411290376397783	0.0
24	3.495551275447216E-4	0.0	0.0	4.59483859609763	0.0
25	3.495551275447216E-4	0.0	0.0	4.772507934106952	0.0
26	3.495551275447216E-4	0.0	0.0	4.967972805882186	0.0
27	3.495551275447216E-4	0.0	0.0	5.174178553394705	0.0
28	3.495551275447216E-4	0.0	0.0	5.3954469491305135	0.0
29	3.813328664124236E-4	0.0	0.0	5.611853350819564	0.0
30	3.813328664124236E-4	0.0	0.0	5.832676858211225	0.0
31	3.813328664124236E-4	0.0	0.0	6.0489561489448045	0.0
32	3.813328664124236E-4	0.0	0.0	6.3135376027572905	0.0
33	3.813328664124236E-4	0.0	0.0	6.556287749967666	0.0
34	3.813328664124236E-4	0.0	0.0	6.844988507580738	0.0
35	3.813328664124236E-4	0.0	0.0	7.082558883355678	0.0
36	3.813328664124236E-4	0.0	0.0	7.367636978737833	0.0
37	3.813328664124236E-4	0.0	0.0	7.705339009684901	0.0
38	3.813328664124236E-4	0.0	0.0	8.111458512414133	0.0
39	3.813328664124236E-4	0.0	0.0	8.538869100184725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAT	35	1.2128294E-7	45.000004	40
ACTACGT	25	3.8923565E-5	45.000004	41
CGTTTTT	27455	0.0	43.942814	1
CGTTTTA	1390	0.0	42.733814	1
TCACGAC	690	0.0	42.717392	24
CGCACTT	9685	0.0	40.818275	34
CCGCACT	9685	0.0	40.702118	33
CCCGCAC	9790	0.0	40.633297	32
ACCCGCA	9750	0.0	40.569233	31
CACGACC	1100	0.0	40.5	26
CTTACTG	9705	0.0	40.386402	38
ATGACGG	340	0.0	40.36765	1
GCACTTA	9800	0.0	40.362244	35
CGCATAC	45	1.9301297E-8	40.000004	42
ATCGGTA	45	1.9301297E-8	40.000004	26
CGGGTAC	560	0.0	39.776787	5
AGACACG	1120	0.0	39.776787	23
ACGGGCT	1745	0.0	39.71347	4
GGTACCT	1810	0.0	39.654694	7
TAGGGTA	960	0.0	39.60938	4
>>END_MODULE