##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043479_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3497497
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.604830826159393	31.0	31.0	33.0	30.0	34.0
2	30.621119045992035	31.0	31.0	34.0	26.0	34.0
3	31.569857243623083	31.0	31.0	34.0	28.0	34.0
4	35.19496885915842	37.0	35.0	37.0	33.0	37.0
5	35.53088451541202	37.0	35.0	37.0	33.0	37.0
6	35.837503506078775	37.0	35.0	37.0	35.0	37.0
7	33.7661742097277	37.0	35.0	37.0	32.0	37.0
8	34.80235780044986	37.0	35.0	37.0	32.0	37.0
9	36.91435046263085	39.0	37.0	39.0	32.0	39.0
10	36.9327095920311	39.0	37.0	39.0	33.0	39.0
11	36.79729646658739	39.0	35.0	39.0	33.0	39.0
12	36.808993974834	39.0	35.0	39.0	33.0	39.0
13	36.81820999417584	39.0	35.0	39.0	33.0	39.0
14	37.996162112505026	40.0	36.0	41.0	33.0	41.0
15	38.09262652691339	40.0	37.0	41.0	33.0	41.0
16	37.94418036670224	40.0	36.0	41.0	33.0	41.0
17	37.83801930351906	40.0	36.0	41.0	33.0	41.0
18	37.583259113588944	39.0	36.0	41.0	33.0	41.0
19	37.30739240090842	38.0	36.0	41.0	33.0	41.0
20	36.9000616726762	38.0	35.0	41.0	32.0	41.0
21	36.84495998138097	38.0	35.0	40.0	32.0	41.0
22	36.813497481198695	38.0	35.0	40.0	32.0	41.0
23	36.75983224574603	38.0	35.0	40.0	32.0	41.0
24	36.63221412341454	38.0	35.0	40.0	32.0	41.0
25	36.53622519190152	38.0	35.0	40.0	32.0	41.0
26	36.27212946858854	37.0	35.0	40.0	31.0	41.0
27	36.13675494217722	37.0	35.0	40.0	31.0	41.0
28	35.96761598365917	37.0	35.0	40.0	31.0	41.0
29	35.852200588020516	37.0	35.0	40.0	30.0	41.0
30	35.66740185910095	37.0	35.0	40.0	30.0	41.0
31	35.3500932238112	37.0	34.0	40.0	29.0	41.0
32	35.138423564051664	37.0	34.0	40.0	26.0	41.0
33	34.65988476902196	37.0	34.0	40.0	24.0	41.0
34	34.48529677080495	37.0	34.0	40.0	23.0	41.0
35	34.3011939681435	37.0	34.0	40.0	22.0	41.0
36	34.16046275379221	36.0	34.0	40.0	21.0	41.0
37	34.012717952295596	36.0	34.0	40.0	21.0	41.0
38	33.86786722047224	36.0	33.0	40.0	20.0	41.0
39	33.731187760847256	36.0	33.0	40.0	18.0	41.0
40	33.61324541522123	35.0	33.0	40.0	18.0	41.0
41	33.5012985000416	35.0	33.0	40.0	18.0	41.0
42	33.382675953689166	35.0	33.0	40.0	18.0	41.0
43	33.19176656906353	35.0	33.0	40.0	18.0	41.0
44	32.96547330848318	35.0	33.0	40.0	17.0	41.0
45	32.74173558976605	35.0	32.0	40.0	17.0	41.0
46	32.733658956676734	35.0	32.0	39.0	18.0	41.0
47	32.70444863855494	35.0	32.0	39.0	18.0	41.0
48	32.595853548980884	35.0	32.0	39.0	18.0	41.0
49	32.53787494313791	35.0	32.0	39.0	17.0	41.0
50	32.45773334473196	35.0	32.0	39.0	17.0	41.0
51	32.05061534005605	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	13.0
12	15.0
13	38.0
14	68.0
15	163.0
16	395.0
17	863.0
18	1744.0
19	3478.0
20	5958.0
21	9631.0
22	15125.0
23	23693.0
24	37596.0
25	58203.0
26	77975.0
27	92923.0
28	98086.0
29	98495.0
30	103416.0
31	115549.0
32	135978.0
33	177424.0
34	306146.0
35	338606.0
36	377606.0
37	374059.0
38	459107.0
39	584815.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.022239046952723	15.960757078562182	22.005880205186738	35.011123669298364
2	36.24208970014842	18.24281764930749	25.78512576279551	19.72996688774858
3	24.225124424695718	19.871039203178732	37.54450682874067	18.359329543384884
4	21.759675562266388	22.33085546606616	35.33910107714174	20.570367894525713
5	19.861375149142372	30.66661672619019	33.85060802053583	15.621400104131611
6	75.7080849533252	1.9749838241462394	20.78223369455356	1.5346975279750061
7	71.32938212670375	7.328583841530099	19.61465585245677	1.7273781793093745
8	69.77372675373275	3.733527148129076	23.047282099169777	3.445463998968405
9	40.067654096629674	18.10646299339213	28.06464165659041	13.76124125338778
10	26.10263854407881	16.458312902055383	36.326521509525236	21.112527044340567
11	23.705038203034913	14.08481551235069	43.03097329318653	19.17917299142787
12	22.050454939632544	15.2963104757488	43.04992970687323	19.60330487774543
13	18.57039477088901	18.396756308868888	44.355863636194684	18.67698528404742
14	17.27815635009837	17.433753338458903	45.96718739144022	19.320902920002506
15	21.6256654401705	15.581886131710762	43.441695589731744	19.350752838386995
16	22.390526710959293	16.12029974578963	40.39703250638957	21.092141036861502
17	21.321733799914625	18.728765171206724	40.9676405726724	18.981860456206253
18	23.074244238093698	22.076273403522574	37.17967449293023	17.669807865453492
19	24.345038751998928	19.561760882139428	36.76509229314564	19.328108072716002
20	23.58555275386941	21.300404260532606	39.10333589993073	16.010707085667264
21	21.82069062532434	17.809336219587895	39.34722460090745	21.022748554180318
22	22.832814438439833	19.406135301903046	38.310054304549794	19.450995955107324
23	22.697374722551587	18.51927249687419	39.13252820517072	19.650824575403494
24	25.18752696571291	17.175311372675946	36.914913722585034	20.722247939026108
25	19.44979509632174	18.412596208088242	38.273227968458585	23.864380727131433
26	17.71295300610694	19.175713374450357	40.45836208008184	22.652971539360863
27	19.352268207806897	19.805335072481835	39.955917045818765	20.8864796738925
28	22.10106255988211	21.099489148954238	36.949109606098304	19.850338685065346
29	20.618859715962586	18.806420706007753	40.464766660271614	20.109952917758044
30	25.148813565815782	18.522331827589845	36.95256922307581	19.376285383518557
31	25.453803105478002	19.690224180320957	35.93792932488577	18.918043389315272
32	25.774489584980344	18.802160516506518	35.59019493083196	19.833154967681175
33	20.892398192192875	20.8033916826805	38.80475094045828	19.49945918466835
34	21.548667518513952	23.421778489016575	35.59219636214127	19.437357630328204
35	24.8936882576311	24.990643308629	32.3484194553991	17.767248978340795
36	23.945352919530738	23.793244140023564	32.148991121364794	20.112411819080904
37	22.371227194762426	27.52122446423828	31.462128487887192	18.645419853112095
38	22.642049442787226	23.025380722270814	32.183730250519154	22.148839584422802
39	21.80733821930369	21.109524897376613	36.594570345592864	20.48856653772684
40	19.927022096087573	21.59621580804787	36.70610725327284	21.770654842591718
41	20.353269781217826	25.013802728065244	31.9242589772057	22.708668513511235
42	19.315270320460602	20.83055396473535	33.75285239701421	26.101323317789838
43	20.502405005636888	20.333855897517566	37.011096792935064	22.152642303910483
44	23.27390130713479	21.98337839889498	34.225647656023725	20.51707263794651
45	22.97854722963308	22.972514343829314	34.079028516679216	19.969909909858394
46	20.864921399503704	23.5784619686593	33.99991479620997	21.55670183562702
47	20.758759764483	24.402222503693356	34.72563378896394	20.11338394285971
48	22.420262261840396	23.269183647619997	34.28594792218549	20.02460616835411
49	20.465206975159663	21.894114562499983	36.364834623160505	21.27584383917985
50	20.742319435870854	21.271726609057847	37.44663683771566	20.53931711735564
51	20.296543499536952	19.790038418903578	36.4982443158636	23.41517376569587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1634.0
1	21933.5
2	42233.0
3	167752.0
4	293271.0
5	176146.5
6	59022.0
7	51890.0
8	44758.0
9	43571.0
10	42384.0
11	41642.0
12	40900.0
13	38946.0
14	36992.0
15	34718.5
16	32445.0
17	30455.0
18	28465.0
19	26490.0
20	24515.0
21	22824.0
22	21133.0
23	20511.0
24	19889.0
25	20257.0
26	21747.5
27	22870.0
28	24558.5
29	26247.0
30	30274.0
31	34301.0
32	38546.0
33	42791.0
34	49888.5
35	56986.0
36	61347.0
37	65708.0
38	67036.0
39	68364.0
40	72042.0
41	75720.0
42	84980.5
43	94241.0
44	104583.0
45	114925.0
46	130671.5
47	146418.0
48	171750.0
49	197082.0
50	199012.0
51	200942.0
52	183389.5
53	165837.0
54	167533.5
55	169230.0
56	179171.0
57	189112.0
58	193353.5
59	197595.0
60	198810.0
61	200025.0
62	198443.0
63	196861.0
64	184043.5
65	171226.0
66	145059.0
67	118892.0
68	96682.5
69	74473.0
70	63967.0
71	53461.0
72	49859.0
73	46257.0
74	38467.0
75	22635.0
76	14593.0
77	10958.0
78	7323.0
79	5617.0
80	3911.0
81	2793.0
82	1675.0
83	1260.5
84	846.0
85	598.0
86	350.0
87	271.0
88	192.0
89	122.5
90	53.0
91	40.0
92	27.0
93	20.0
94	13.0
95	10.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3497497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.831211070510097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47924158798179	21.00678704077145
2	8.517867620719839	4.741251432458351
3	2.914571854863019	2.4334819341858247
4	1.6608303109321678	1.8489167574341634
5	1.2042301451644148	1.6757591673770924
6	0.8975197488753676	1.4987436942540946
7	0.8100626896054149	1.5781517986327387
8	0.6548877311089902	1.4581054937585418
9	0.6003396616479301	1.5037361853589943
>10	6.794045112759893	40.4079877128461
>50	0.3844034523939938	6.866032077808207
>100	0.0708754623244781	3.6441186057585444
>500	0.006804380215844298	1.2800790216966613
>1k	0.0034561931254992943	1.6154816687309443
>5k	4.320241406874118E-4	0.8264528031574082
>10k+	4.320241406874118E-4	7.614914605770825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	194902	5.572613786373512	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	27693	0.7917948178368703	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19875	0.5682635324633588	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10859	0.3104791798248862	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8742	0.24995017865633626	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8359	0.23899949020685365	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5362	0.1533096382927562	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5031	0.1438457273873287	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4546	0.1299786676014304	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4422	0.126433274996376	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05012155836016443	0.0
2	0.0	0.0	0.0	0.15362414892707557	0.0
3	0.0	0.0	0.0	0.25369571439232114	0.0
4	0.0	0.0	0.0	0.32506103650696483	0.0
5	0.0	0.0	0.0	0.7035316970965236	0.0
6	0.0	0.0	0.0	0.9392145297051006	0.0
7	0.0	0.0	0.0	1.4574422794358366	0.0
8	0.0	0.0	0.0	2.0489224150871324	0.0
9	0.0	0.0	0.0	2.623104465850864	0.0
10	0.0	0.0	0.0	2.9493091773917177	0.0
11	0.0	0.0	0.0	3.232311564527432	0.0
12	0.0	0.0	0.0	3.4400029506815875	0.0
13	0.0	0.0	0.0	3.553912984056884	0.0
14	0.0	0.0	0.0	3.6360860352417745	0.0
15	0.0	0.0	0.0	3.811039723550871	0.0
16	0.0	0.0	0.0	3.9919405220361877	0.0
17	0.0	0.0	0.0	4.265621957645711	0.0
18	0.0	0.0	0.0	4.404063820497916	0.0
19	0.0	0.0	0.0	4.586765907161607	0.0
20	0.0	0.0	0.0	4.785679587430669	0.0
21	0.0	0.0	0.0	5.016330249890136	0.0
22	0.0	0.0	0.0	5.217445504599432	0.0
23	0.0	0.0	0.0	5.404865250777913	0.0
24	0.0	0.0	0.0	5.556945438409239	0.0
25	0.0	0.0	0.0	5.715973451871438	0.0
26	0.0	0.0	0.0	5.883464660584412	0.0
27	0.0	0.0	0.0	6.05953343205155	0.0
28	0.0	0.0	0.0	6.230741584624662	0.0
29	0.0	0.0	0.0	6.4145015706947	0.0
30	0.0	0.0	0.0	6.595917022945266	0.0
31	0.0	0.0	0.0	6.786167364832622	0.0
32	0.0	0.0	0.0	7.013701512824743	0.0
33	0.0	0.0	0.0	7.216560871960719	0.0
34	0.0	0.0	0.0	7.463251576770474	0.0
35	0.0	0.0	0.0	7.6818078757465695	0.0
36	0.0	0.0	0.0	7.939163350247334	0.0
37	0.0	0.0	0.0	8.265167918657257	0.0
38	0.0	0.0	0.0	8.624939492442738	0.0
39	0.0	0.0	0.0	9.015561700267362	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	51385	0.0	44.097984	1
CGTTTTA	2740	0.0	42.37226	1
TATACGG	145	0.0	41.896553	1
TCACGAC	710	0.0	40.56338	24
GCACTTA	8685	0.0	40.46632	35
CGCACTT	8695	0.0	40.445656	34
ACTACGG	195	0.0	40.384617	1
CACTTAC	8715	0.0	40.35284	36
CGGTCTA	720	0.0	40.3125	30
GCATAGG	470	0.0	40.212765	1
CTTACTG	8655	0.0	40.16465	38
ACCCGCA	8775	0.0	40.102566	31
CCCGCAC	8850	0.0	40.04237	32
CCGCACT	8820	0.0	39.974487	33
ACTTACT	8735	0.0	39.87407	37
ACGGGCT	1920	0.0	39.374996	4
CACGGGC	1230	0.0	39.146343	3
AATGCGG	420	0.0	39.107143	1
CAACGCA	9500	0.0	38.842106	16
AACGCAA	9505	0.0	38.798	17
>>END_MODULE