FastQCFastQC Report
Thu 26 May 2016
SRR1043478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043478_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3184942
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1225713.8484531272469016No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC238860.7499665614004901No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133270.4184377611899997No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC87740.2754838235672738No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC67120.21074167127690238No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54400.17080373834123197No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA46370.1455913482882891No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC41940.13168214680204537No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC40720.1278516217877751No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT350000.044.1064261
TCACGAC6150.042.43902624
CGGTCTA6200.041.73387530
TATACGG1950.041.538461
ACTTACT73400.041.01498437
CGCACTT73800.041.006134
ATTGCGG6750.041.01
TTAACGG2200.040.9090921
CTTACTG72900.040.86419738
CACTTAC74000.040.77364736
ACCCGCA74450.040.7387531
CCGCACT74250.040.69696833
CCCGCAC75150.040.6586832
CGACGGT6450.040.4651227
GCACTTA74350.040.43039735
CACGACC10950.040.2739726
CGTTTTA19100.039.8167531
CACGACG6500.039.8076925
TACGGGA6350.039.685043
TTATGAC79350.039.55576726