##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043478_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3184942
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.517600948463112	31.0	31.0	33.0	30.0	34.0
2	30.52679106872276	31.0	31.0	34.0	26.0	34.0
3	31.50341356294714	31.0	31.0	34.0	28.0	34.0
4	35.124310270014334	37.0	35.0	37.0	32.0	37.0
5	35.447400298027404	37.0	35.0	37.0	33.0	37.0
6	35.7763708098923	37.0	35.0	37.0	35.0	37.0
7	33.67612094662948	37.0	35.0	37.0	32.0	37.0
8	34.706531861490724	37.0	35.0	37.0	31.0	37.0
9	36.74471842815348	39.0	37.0	39.0	32.0	39.0
10	36.721359761025475	39.0	35.0	39.0	32.0	39.0
11	36.495846706156655	39.0	35.0	39.0	32.0	39.0
12	36.501154809098566	39.0	35.0	39.0	32.0	39.0
13	36.50924381040534	39.0	35.0	39.0	32.0	39.0
14	37.738748146748044	40.0	36.0	41.0	33.0	41.0
15	37.81669556305892	40.0	36.0	41.0	33.0	41.0
16	37.60446249884613	40.0	36.0	41.0	32.0	41.0
17	37.58066771702593	40.0	36.0	41.0	32.0	41.0
18	37.37215245991921	39.0	36.0	41.0	32.0	41.0
19	37.1868191006304	38.0	35.0	41.0	32.0	41.0
20	36.92786430647717	38.0	35.0	41.0	32.0	41.0
21	36.80151004319702	38.0	35.0	40.0	32.0	41.0
22	36.73693963657737	38.0	35.0	40.0	32.0	41.0
23	36.67963184258928	38.0	35.0	40.0	32.0	41.0
24	36.54887498736241	38.0	35.0	40.0	32.0	41.0
25	36.43712098995837	38.0	35.0	40.0	31.0	41.0
26	36.17713823360049	37.0	35.0	40.0	31.0	41.0
27	36.04018346330954	37.0	35.0	40.0	31.0	41.0
28	35.89096787319832	37.0	35.0	40.0	30.0	41.0
29	35.755132432552934	37.0	35.0	40.0	30.0	41.0
30	35.571566138410056	37.0	34.0	40.0	29.0	41.0
31	35.33523938583497	37.0	34.0	40.0	28.0	41.0
32	35.191543205496366	37.0	34.0	40.0	27.0	41.0
33	34.876535899240864	37.0	34.0	40.0	25.0	41.0
34	34.71963853658873	37.0	34.0	40.0	24.0	41.0
35	34.56491421193855	37.0	34.0	40.0	23.0	41.0
36	34.451317166843225	37.0	34.0	40.0	23.0	41.0
37	34.31040565259901	36.0	34.0	40.0	22.0	41.0
38	34.1845210995993	36.0	34.0	40.0	22.0	41.0
39	34.06026514768558	36.0	33.0	40.0	22.0	41.0
40	33.966664699074585	36.0	33.0	40.0	21.0	41.0
41	33.87074992260455	35.0	33.0	40.0	21.0	41.0
42	33.75489506559303	35.0	33.0	40.0	21.0	41.0
43	33.57674456866091	35.0	33.0	40.0	20.0	41.0
44	33.35053040212349	35.0	33.0	40.0	19.0	41.0
45	33.12958540532292	35.0	33.0	40.0	19.0	41.0
46	33.121771762248734	35.0	33.0	40.0	20.0	41.0
47	33.08471520046519	35.0	33.0	39.0	20.0	41.0
48	32.996924276800016	35.0	33.0	39.0	20.0	41.0
49	32.95449242089809	35.0	33.0	39.0	20.0	41.0
50	32.87250380069715	35.0	33.0	39.0	20.0	41.0
51	32.434108062250424	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	13.0
12	19.0
13	35.0
14	87.0
15	185.0
16	433.0
17	948.0
18	1835.0
19	3410.0
20	6021.0
21	9564.0
22	14730.0
23	22023.0
24	34025.0
25	50280.0
26	65785.0
27	77290.0
28	81885.0
29	86086.0
30	93590.0
31	106712.0
32	127669.0
33	167454.0
34	294078.0
35	315861.0
36	317020.0
37	340708.0
38	423227.0
39	543654.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.26304466454962	16.602876912672194	23.69999202497251	30.434086397805675
2	32.73343753198645	20.004854091534476	27.029032239833565	20.2326761366455
3	25.87852463247368	20.892499769226568	34.54621779611685	18.6827578021829
4	23.3960932412584	24.132652965108942	31.261040232443793	21.21021356118887
5	21.280984080714813	32.14658226115263	29.91809583973586	16.65433781839669
6	79.51259394990552	2.358535885425857	16.621872549013453	1.5069976156551674
7	75.14887241274724	7.646575667625973	15.464049266831234	1.740502652795561
8	73.82665053241158	3.9654725266582562	18.799494621880083	3.40838231905008
9	42.885961502595656	18.29138489806094	24.655739413778964	14.166914185564448
10	29.897467520601634	19.070268783544567	31.437809542528562	19.59445415332524
11	26.401830865365838	15.99087204727747	39.23176622996588	18.37553085739081
12	22.272587695474517	17.501982767661076	39.502760175852494	20.72266936101191
13	17.983373009618386	20.544204572642137	42.19718914818543	19.275233269554047
14	19.30267489957431	18.708284169696025	41.99172229823965	19.99731863249001
15	24.105054346358585	15.628699046952818	40.51266867654105	19.75357793014755
16	23.027766282714097	16.8920815512496	37.976704128364034	22.103448037672273
17	24.06411168555032	19.357777943836968	36.63580058914731	19.942309781465408
18	23.998992760307722	23.44001868793843	33.811102368583164	18.749886183170684
19	25.60062318246298	20.96716360925882	33.132754065851124	20.299459142427086
20	25.577420248155224	22.78958298141693	34.74898443990503	16.884012330522815
21	21.986334445022862	18.77676265376261	37.20614064557534	22.030762255639193
22	23.811328432354497	20.367749240017556	35.69725916515905	20.123663162468894
23	24.45884414849627	20.118608125359895	36.33683125155811	19.085716474585723
24	25.39996018765805	18.30234271142143	34.52819548990217	21.76950161101835
25	20.267590430218196	20.075593213314402	36.16423784169382	23.492578514773584
26	18.864330967408513	19.444341529610274	37.219076516935004	24.472250986046216
27	19.98607823941535	22.522074185338383	36.90663754630383	20.58521002894244
28	22.37996798685816	22.659973085852116	34.39120712402298	20.568851803266746
29	22.53048878127137	20.05936057862278	38.381860642988165	19.028289997117685
30	26.9303805218431	20.51626057868558	33.9081841992727	18.645174700198623
31	27.19481233881182	20.858244828320263	32.67189794978998	19.275044883077932
32	26.56557011085288	20.50759480078444	31.646824337774436	21.28001075058824
33	22.27472274220378	22.41858721446105	35.11300990724478	20.193680136090393
34	21.968186547824107	24.020594409568528	33.7480556945778	20.263163348029572
35	24.046748732002026	27.46542951174621	30.22067591811719	18.26714583813457
36	23.981723999997488	25.946846127810176	29.26781712194445	20.803612750247886
37	23.61791831687987	28.509435964610972	29.0094136722113	18.86323204629786
38	23.867310613505676	24.73143937942983	28.734840383278566	22.666409623785928
39	23.074674515265897	22.273309843632948	32.65403263230539	21.997983008795767
40	20.95272064609026	23.270439461691925	32.665492809602185	23.111347082615634
41	20.27336761548562	26.22845251185108	29.45058340151877	24.04759647114453
42	19.925449191853414	21.886332623953592	31.393632913880374	26.794585270312616
43	21.16889412742838	21.08094904083026	33.59307642022994	24.15708041151142
44	23.479611245667897	23.103654634841075	31.94839968828318	21.468334431207854
45	24.189231703434473	23.875254243248385	31.524875492238163	20.41063856107898
46	21.91076007035607	23.748344553841168	31.591846884495855	22.749048491306905
47	20.515475635035113	25.204415025454153	33.43728080448561	20.842828535025127
48	22.877465272523015	24.26241984940385	32.14199819023392	20.718116687839213
49	21.37065604334396	22.204234802392005	33.87320711020797	22.55190204405606
50	20.228186258964843	23.027075532301687	35.56570260934108	21.17903559939239
51	19.752792986497088	21.300984444928666	34.105770214967805	24.84045235360644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1450.0
1	15913.0
2	30376.0
3	109863.5
4	189351.0
5	115387.0
6	41423.0
7	36966.5
8	32510.0
9	32202.0
10	31894.0
11	31627.0
12	31360.0
13	30260.5
14	29161.0
15	26708.0
16	24255.0
17	22486.0
18	20717.0
19	19165.5
20	17614.0
21	17328.5
22	17043.0
23	16080.0
24	15117.0
25	16181.0
26	19549.0
27	21853.0
28	22900.0
29	23947.0
30	27626.5
31	31306.0
32	36773.0
33	42240.0
34	48487.0
35	54734.0
36	57554.5
37	60375.0
38	65246.5
39	70118.0
40	78969.0
41	87820.0
42	101764.0
43	115708.0
44	128640.5
45	141573.0
46	153796.0
47	166019.0
48	182185.5
49	198352.0
50	192086.0
51	185820.0
52	172420.0
53	159020.0
54	157357.5
55	155695.0
56	161451.0
57	167207.0
58	174584.5
59	181962.0
60	181381.0
61	180800.0
62	178530.5
63	176261.0
64	161500.0
65	146739.0
66	120559.0
67	94379.0
68	80002.0
69	65625.0
70	58296.5
71	50968.0
72	46927.5
73	42887.0
74	36931.5
75	24310.0
76	17644.0
77	12590.5
78	7537.0
79	5929.0
80	4321.0
81	3189.0
82	2057.0
83	1447.5
84	838.0
85	619.0
86	400.0
87	274.5
88	149.0
89	101.5
90	54.0
91	41.0
92	28.0
93	17.5
94	7.0
95	4.0
96	1.0
97	3.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3184942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.19579471239173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56137929272884	21.093433488550044
2	9.131603519836675	4.9668242944086405
3	3.025779129914088	2.4686540418654843
4	1.4633235695045232	1.5918498957619722
5	0.9089858590615691	1.236029640975274
6	0.6671162871776078	1.0885654557225117
7	0.49302416120631504	0.938572871349125
8	0.4042176700620854	0.8794416619303815
9	0.344887111275297	0.8441531169473502
>10	4.859914045465149	31.81517377155558
>50	0.964124621744728	17.48591584584713
>100	0.16591169367216824	7.046106091008718
>500	0.006652962923450726	1.2178440217135509
>1k	0.0023408573249178457	1.2725593843970788
>5k	3.696090513028177E-4	0.7011473848839724
>10k+	3.696090513028177E-4	5.353729033083277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122571	3.8484531272469016	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	23886	0.7499665614004901	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13327	0.4184377611899997	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8774	0.2754838235672738	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6712	0.21074167127690238	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5440	0.17080373834123197	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4637	0.1455913482882891	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4194	0.13168214680204537	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4072	0.1278516217877751	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05434949835821186	0.0
2	0.0	0.0	0.0	0.17366093322892537	0.0
3	0.0	0.0	0.0	0.27334877683800834	0.0
4	0.0	0.0	0.0	0.33887587277884496	0.0
5	0.0	0.0	0.0	0.7094948667825034	0.0
6	0.0	0.0	0.0	0.8484298929148474	0.0
7	0.0	0.0	0.0	1.2308230416754842	0.0
8	0.0	0.0	0.0	1.6442999589945437	0.0
9	0.0	0.0	0.0	2.046222505778755	0.0
10	0.0	0.0	0.0	2.2932285737071507	0.0
11	0.0	0.0	0.0	2.5486492375685335	0.0
12	0.0	0.0	0.0	2.7570674756400586	0.0
13	0.0	0.0	0.0	2.865797870102501	0.0
14	0.0	0.0	0.0	2.944480621625135	0.0
15	0.0	0.0	0.0	3.16621150400855	0.0
16	0.0	0.0	0.0	3.422008940822156	0.0
17	0.0	0.0	0.0	3.7465988391625342	0.0
18	0.0	0.0	0.0	3.9370575665114154	0.0
19	0.0	0.0	0.0	4.167203044827818	0.0
20	0.0	0.0	0.0	4.418447808468725	0.0
21	0.0	0.0	0.0	4.673428903885847	0.0
22	0.0	0.0	0.0	4.922507223051472	0.0
23	0.0	0.0	0.0	5.160062569428266	0.0
24	3.139774601860882E-5	0.0	0.0	5.357648585123371	0.0
25	3.139774601860882E-5	0.0	0.0	5.557966204722096	0.0
26	3.139774601860882E-5	0.0	0.0	5.779477302883381	0.0
27	3.139774601860882E-5	0.0	0.0	6.012291589611365	0.0
28	3.139774601860882E-5	0.0	0.0	6.251793596241313	0.0
29	3.139774601860882E-5	0.0	0.0	6.5101028527364075	0.0
30	3.139774601860882E-5	0.0	0.0	6.753843555078868	0.0
31	3.139774601860882E-5	0.0	0.0	7.002419510308194	0.0
32	3.139774601860882E-5	0.0	0.0	7.291404364663469	0.0
33	3.139774601860882E-5	0.0	0.0	7.573324726164558	0.0
34	3.139774601860882E-5	0.0	0.0	7.888275516477223	0.0
35	3.139774601860882E-5	0.0	0.0	8.169850502772107	0.0
36	3.139774601860882E-5	0.0	0.0	8.458395788683122	0.0
37	3.139774601860882E-5	0.0	0.0	8.83127542040012	0.0
38	3.139774601860882E-5	0.0	0.0	9.215835013636042	0.0
39	3.139774601860882E-5	0.0	0.0	9.691259683849816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35000	0.0	44.106426	1
TCACGAC	615	0.0	42.439026	24
CGGTCTA	620	0.0	41.733875	30
TATACGG	195	0.0	41.53846	1
ACTTACT	7340	0.0	41.014984	37
CGCACTT	7380	0.0	41.0061	34
ATTGCGG	675	0.0	41.0	1
TTAACGG	220	0.0	40.909092	1
CTTACTG	7290	0.0	40.864197	38
CACTTAC	7400	0.0	40.773647	36
ACCCGCA	7445	0.0	40.73875	31
CCGCACT	7425	0.0	40.696968	33
CCCGCAC	7515	0.0	40.65868	32
CGACGGT	645	0.0	40.46512	27
GCACTTA	7435	0.0	40.430397	35
CACGACC	1095	0.0	40.27397	26
CGTTTTA	1910	0.0	39.816753	1
CACGACG	650	0.0	39.80769	25
TACGGGA	635	0.0	39.68504	3
TTATGAC	7935	0.0	39.555767	26
>>END_MODULE