FastQCFastQC Report
Thu 26 May 2016
SRR1043477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043477_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences561803
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432957.706438021868876No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47430.8442461147412882No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.3292969243667264No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.14506864505885517No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC6330.11267294763466909TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT5720.10181504904744189Illumina PCR Primer Index 8 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTGG453.8380676E-1045.0000041
GTCTAGG650.045.0000041
AATGCGG1300.045.0000041
GCGATAG453.8380676E-1045.0000048
ACGTAGG453.8380676E-1045.0000041
ATCGGTC453.8380676E-1045.00000413
ATCGCGG453.8380676E-1045.0000041
AGTACGG453.8380676E-1045.0000041
GCATAAC406.8048394E-945.08
TCGCCGT207.030205E-445.024
AACGTGT207.030205E-445.019
AGTCCGT253.8882514E-545.029
ACACGGC207.030205E-445.02
TCACCTA207.030205E-445.035
ACGCCAT207.030205E-445.042
CACGGCA207.030205E-445.03
CGTGCTA207.030205E-445.038
CGACGGT551.8189894E-1245.027
GCGGTGA207.030205E-445.023
ACGTTCT207.030205E-445.044